Mycobacterium phage Anthony

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fromanvirus; unclassified Fromanvirus

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 89 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5J6THW1|A0A5J6THW1_9CAUD Uncharacterized protein OS=Mycobacterium phage Anthony OX=2599857 GN=91 PE=4 SV=1
MM1 pKa = 7.49TPQFSLDD8 pKa = 3.66GGKK11 pKa = 8.41TNYY14 pKa = 10.25DD15 pKa = 3.06AVSYY19 pKa = 9.45GRR21 pKa = 11.84PWNGWATPVVTRR33 pKa = 11.84EE34 pKa = 3.85TLEE37 pKa = 3.93RR38 pKa = 11.84LAATEE43 pKa = 4.24GNNGQYY49 pKa = 10.94VSLSFDD55 pKa = 3.35GDD57 pKa = 3.65TATLIEE63 pKa = 5.62LADD66 pKa = 3.5EE67 pKa = 4.17WRR69 pKa = 11.84NEE71 pKa = 3.67CRR73 pKa = 11.84FVLFPDD79 pKa = 3.28EE80 pKa = 4.1SGYY83 pKa = 11.44YY84 pKa = 9.94DD85 pKa = 4.51LSYY88 pKa = 11.03LGWTFYY94 pKa = 10.85KK95 pKa = 10.81VEE97 pKa = 4.18DD98 pKa = 3.63

Molecular weight: 11.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5J6TNU6|A0A5J6TNU6_9CAUD Minor tail protein OS=Mycobacterium phage Anthony OX=2599857 GN=30 PE=4 SV=1
MM1 pKa = 6.96AQVYY5 pKa = 10.13RR6 pKa = 11.84NAGRR10 pKa = 11.84AAARR14 pKa = 11.84HH15 pKa = 4.99VEE17 pKa = 3.75TRR19 pKa = 11.84RR20 pKa = 11.84RR21 pKa = 11.84VRR23 pKa = 11.84RR24 pKa = 11.84VARR27 pKa = 11.84GVEE30 pKa = 3.63HH31 pKa = 7.47RR32 pKa = 11.84ARR34 pKa = 11.84INLLRR39 pKa = 11.84ANKK42 pKa = 9.37SEE44 pKa = 4.73RR45 pKa = 11.84ITATGYY51 pKa = 10.06FPAFIDD57 pKa = 3.67SAEE60 pKa = 4.14KK61 pKa = 10.62GEE63 pKa = 4.74SVFIYY68 pKa = 10.51LNAPNPVALEE78 pKa = 4.14FGHH81 pKa = 6.13MPSGVFAGTDD91 pKa = 3.24TKK93 pKa = 11.22APEE96 pKa = 4.06GLYY99 pKa = 10.2ILIRR103 pKa = 11.84AAYY106 pKa = 9.39ASEE109 pKa = 4.11KK110 pKa = 10.76

Molecular weight: 12.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

89

0

89

16398

37

960

184.2

20.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.861 ± 0.353

0.89 ± 0.129

6.598 ± 0.172

6.543 ± 0.216

3.476 ± 0.163

8.123 ± 0.354

1.769 ± 0.205

5.226 ± 0.163

4.952 ± 0.251

8.123 ± 0.215

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.427 ± 0.104

3.464 ± 0.16

5.184 ± 0.233

3.391 ± 0.199

6.281 ± 0.339

5.86 ± 0.19

6.068 ± 0.238

6.861 ± 0.199

1.878 ± 0.128

3.025 ± 0.191
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski