Amino acid dipepetide frequency for Sphaerobacter thermophilus (strain DSM 20745 / S 6022)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	17.308AlaAla: 17.308 ± 0.192	0.928AlaCys: 0.928 ± 0.033	6.27AlaAsp: 6.27 ± 0.072	8.053AlaGlu: 8.053 ± 0.102	3.558AlaPhe: 3.558 ± 0.062	10.657AlaGly: 10.657 ± 0.129	2.083AlaHis: 2.083 ± 0.049	6.213AlaIle: 6.213 ± 0.098	1.93AlaLys: 1.93 ± 0.044	13.696AlaLeu: 13.696 ± 0.143	2.745AlaMet: 2.745 ± 0.053	2.191AlaAsn: 2.191 ± 0.048	5.626AlaPro: 5.626 ± 0.083	3.53AlaGln: 3.53 ± 0.067	10.178AlaArg: 10.178 ± 0.109	5.148AlaSer: 5.148 ± 0.073	6.647AlaThr: 6.647 ± 0.088	10.276AlaVal: 10.276 ± 0.109	1.645AlaTrp: 1.645 ± 0.045	2.675AlaTyr: 2.675 ± 0.049	0.0AlaXaa: 0.0 ± 0.0
Cys	0.706CysAla: 0.706 ± 0.022	0.094CysCys: 0.094 ± 0.009	0.447CysAsp: 0.447 ± 0.019	0.331CysGlu: 0.331 ± 0.016	0.217CysPhe: 0.217 ± 0.014	0.845CysGly: 0.845 ± 0.031	0.188CysHis: 0.188 ± 0.014	0.247CysIle: 0.247 ± 0.017	0.087CysLys: 0.087 ± 0.009	0.639CysLeu: 0.639 ± 0.025	0.094CysMet: 0.094 ± 0.009	0.145CysAsn: 0.145 ± 0.013	0.494CysPro: 0.494 ± 0.023	0.229CysGln: 0.229 ± 0.013	0.567CysArg: 0.567 ± 0.023	0.301CysSer: 0.301 ± 0.017	0.36CysThr: 0.36 ± 0.021	0.49CysVal: 0.49 ± 0.023	0.098CysTrp: 0.098 ± 0.008	0.21CysTyr: 0.21 ± 0.013	0.0CysXaa: 0.0 ± 0.0
Asp	6.489AspAla: 6.489 ± 0.092	0.334AspCys: 0.334 ± 0.017	3.24AspAsp: 3.24 ± 0.068	4.02AspGlu: 4.02 ± 0.059	1.668AspPhe: 1.668 ± 0.039	5.245AspGly: 5.245 ± 0.074	1.105AspHis: 1.105 ± 0.032	2.5AspIle: 2.5 ± 0.05	0.799AspLys: 0.799 ± 0.029	6.624AspLeu: 6.624 ± 0.085	0.92AspMet: 0.92 ± 0.029	0.963AspAsn: 0.963 ± 0.032	4.576AspPro: 4.576 ± 0.065	1.751AspGln: 1.751 ± 0.043	4.622AspArg: 4.622 ± 0.061	1.812AspSer: 1.812 ± 0.038	2.599AspThr: 2.599 ± 0.053	4.607AspVal: 4.607 ± 0.062	0.828AspTrp: 0.828 ± 0.027	1.47AspTyr: 1.47 ± 0.04	0.0AspXaa: 0.0 ± 0.0
Glu	8.044GluAla: 8.044 ± 0.1	0.288GluCys: 0.288 ± 0.014	2.767GluAsp: 2.767 ± 0.06	4.626GluGlu: 4.626 ± 0.078	1.755GluPhe: 1.755 ± 0.04	4.362GluGly: 4.362 ± 0.07	1.577GluHis: 1.577 ± 0.044	3.649GluIle: 3.649 ± 0.066	1.137GluLys: 1.137 ± 0.04	5.784GluLeu: 5.784 ± 0.09	1.523GluMet: 1.523 ± 0.041	1.153GluAsn: 1.153 ± 0.036	3.908GluPro: 3.908 ± 0.066	2.852GluGln: 2.852 ± 0.059	7.249GluArg: 7.249 ± 0.099	2.54GluSer: 2.54 ± 0.048	3.274GluThr: 3.274 ± 0.052	5.26GluVal: 5.26 ± 0.072	0.861GluTrp: 0.861 ± 0.029	1.36GluTyr: 1.36 ± 0.037	0.0GluXaa: 0.0 ± 0.0
Phe	3.556PheAla: 3.556 ± 0.055	0.239PheCys: 0.239 ± 0.013	2.12PheAsp: 2.12 ± 0.046	1.769PheGlu: 1.769 ± 0.04	1.12PhePhe: 1.12 ± 0.035	3.18PheGly: 3.18 ± 0.053	0.677PheHis: 0.677 ± 0.026	1.429PheIle: 1.429 ± 0.036	0.523PheLys: 0.523 ± 0.025	3.125PheLeu: 3.125 ± 0.062	0.502PheMet: 0.502 ± 0.021	0.789PheAsn: 0.789 ± 0.025	1.712PhePro: 1.712 ± 0.038	0.942PheGln: 0.942 ± 0.031	2.214PheArg: 2.214 ± 0.045	1.516PheSer: 1.516 ± 0.034	1.905PheThr: 1.905 ± 0.048	2.593PheVal: 2.593 ± 0.059	0.564PheTrp: 0.564 ± 0.021	0.875PheTyr: 0.875 ± 0.027	0.0PheXaa: 0.0 ± 0.0
Gly	9.019GlyAla: 9.019 ± 0.108	0.683GlyCys: 0.683 ± 0.025	4.597GlyAsp: 4.597 ± 0.059	5.142GlyGlu: 5.142 ± 0.071	2.986GlyPhe: 2.986 ± 0.06	7.154GlyGly: 7.154 ± 0.109	1.91GlyHis: 1.91 ± 0.046	4.712GlyIle: 4.712 ± 0.077	1.95GlyLys: 1.95 ± 0.044	8.613GlyLeu: 8.613 ± 0.098	2.107GlyMet: 2.107 ± 0.048	1.842GlyAsn: 1.842 ± 0.038	4.254GlyPro: 4.254 ± 0.06	2.868GlyGln: 2.868 ± 0.05	6.835GlyArg: 6.835 ± 0.083	4.376GlySer: 4.376 ± 0.065	5.347GlyThr: 5.347 ± 0.081	7.268GlyVal: 7.268 ± 0.087	1.505GlyTrp: 1.505 ± 0.042	2.532GlyTyr: 2.532 ± 0.048	0.0GlyXaa: 0.0 ± 0.0
His	2.172HisAla: 2.172 ± 0.046	0.175HisCys: 0.175 ± 0.013	1.226HisAsp: 1.226 ± 0.03	1.127HisGlu: 1.127 ± 0.031	0.69HisPhe: 0.69 ± 0.024	1.945HisGly: 1.945 ± 0.038	0.564HisHis: 0.564 ± 0.026	0.858HisIle: 0.858 ± 0.029	0.271HisLys: 0.271 ± 0.017	2.363HisLeu: 2.363 ± 0.048	0.33HisMet: 0.33 ± 0.018	0.445HisAsn: 0.445 ± 0.02	1.64HisPro: 1.64 ± 0.04	0.635HisGln: 0.635 ± 0.022	1.725HisArg: 1.725 ± 0.04	0.785HisSer: 0.785 ± 0.026	1.018HisThr: 1.018 ± 0.031	1.603HisVal: 1.603 ± 0.039	0.314HisTrp: 0.314 ± 0.016	0.608HisTyr: 0.608 ± 0.024	0.0HisXaa: 0.0 ± 0.0
Ile	6.558IleAla: 6.558 ± 0.084	0.376IleCys: 0.376 ± 0.018	3.459IleAsp: 3.459 ± 0.056	3.432IleGlu: 3.432 ± 0.062	1.5IlePhe: 1.5 ± 0.043	4.56IleGly: 4.56 ± 0.076	0.996IleHis: 0.996 ± 0.033	2.184IleIle: 2.184 ± 0.046	0.899IleLys: 0.899 ± 0.032	4.509IleLeu: 4.509 ± 0.065	0.724IleMet: 0.724 ± 0.025	1.186IleAsn: 1.186 ± 0.039	2.983IlePro: 2.983 ± 0.053	1.448IleGln: 1.448 ± 0.038	3.445IleArg: 3.445 ± 0.06	2.221IleSer: 2.221 ± 0.052	2.977IleThr: 2.977 ± 0.058	4.453IleVal: 4.453 ± 0.065	0.608IleTrp: 0.608 ± 0.022	1.208IleTyr: 1.208 ± 0.034	0.0IleXaa: 0.0 ± 0.0
Lys	1.808LysAla: 1.808 ± 0.048	0.077LysCys: 0.077 ± 0.008	0.844LysAsp: 0.844 ± 0.03	1.088LysGlu: 1.088 ± 0.035	0.431LysPhe: 0.431 ± 0.02	1.283LysGly: 1.283 ± 0.04	0.368LysHis: 0.368 ± 0.019	0.932LysIle: 0.932 ± 0.029	0.403LysLys: 0.403 ± 0.023	1.62LysLeu: 1.62 ± 0.046	0.34LysMet: 0.34 ± 0.018	0.376LysAsn: 0.376 ± 0.02	1.168LysPro: 1.168 ± 0.035	0.59LysGln: 0.59 ± 0.027	1.485LysArg: 1.485 ± 0.038	0.773LysSer: 0.773 ± 0.028	1.069LysThr: 1.069 ± 0.034	1.416LysVal: 1.416 ± 0.041	0.203LysTrp: 0.203 ± 0.014	0.427LysTyr: 0.427 ± 0.021	0.0LysXaa: 0.0 ± 0.0
Leu	14.587LeuAla: 14.587 ± 0.163	0.657LeuCys: 0.657 ± 0.027	6.139LeuAsp: 6.139 ± 0.092	5.841LeuGlu: 5.841 ± 0.091	3.018LeuPhe: 3.018 ± 0.062	9.14LeuGly: 9.14 ± 0.097	1.987LeuHis: 1.987 ± 0.038	5.076LeuIle: 5.076 ± 0.072	1.728LeuLys: 1.728 ± 0.043	11.541LeuLeu: 11.541 ± 0.143	1.927LeuMet: 1.927 ± 0.04	2.042LeuAsn: 2.042 ± 0.046	6.147LeuPro: 6.147 ± 0.073	2.832LeuGln: 2.832 ± 0.054	8.965LeuArg: 8.965 ± 0.094	5.189LeuSer: 5.189 ± 0.075	6.135LeuThr: 6.135 ± 0.081	8.86LeuVal: 8.86 ± 0.101	1.324LeuTrp: 1.324 ± 0.036	2.326LeuTyr: 2.326 ± 0.051	0.0LeuXaa: 0.0 ± 0.0
Met	2.545MetAla: 2.545 ± 0.047	0.104MetCys: 0.104 ± 0.01	0.968MetAsp: 0.968 ± 0.027	1.054MetGlu: 1.054 ± 0.032	0.483MetPhe: 0.483 ± 0.019	1.452MetGly: 1.452 ± 0.041	0.387MetHis: 0.387 ± 0.017	1.075MetIle: 1.075 ± 0.032	0.393MetLys: 0.393 ± 0.018	2.14MetLeu: 2.14 ± 0.045	0.451MetMet: 0.451 ± 0.02	0.44MetAsn: 0.44 ± 0.019	1.252MetPro: 1.252 ± 0.028	0.658MetGln: 0.658 ± 0.024	1.72MetArg: 1.72 ± 0.044	1.169MetSer: 1.169 ± 0.033	1.452MetThr: 1.452 ± 0.034	1.62MetVal: 1.62 ± 0.037	0.166MetTrp: 0.166 ± 0.011	0.367MetTyr: 0.367 ± 0.019	0.0MetXaa: 0.0 ± 0.0
Asn	2.306AsnAla: 2.306 ± 0.047	0.166AsnCys: 0.166 ± 0.011	0.998AsnAsp: 0.998 ± 0.032	1.073AsnGlu: 1.073 ± 0.031	0.633AsnPhe: 0.633 ± 0.027	1.935AsnGly: 1.935 ± 0.044	0.397AsnHis: 0.397 ± 0.018	1.01AsnIle: 1.01 ± 0.03	0.335AsnLys: 0.335 ± 0.018	2.241AsnLeu: 2.241 ± 0.046	0.357AsnMet: 0.357 ± 0.017	0.468AsnAsn: 0.468 ± 0.027	1.835AsnPro: 1.835 ± 0.048	0.66AsnGln: 0.66 ± 0.029	1.581AsnArg: 1.581 ± 0.037	0.831AsnSer: 0.831 ± 0.03	1.109AsnThr: 1.109 ± 0.031	1.728AsnVal: 1.728 ± 0.042	0.326AsnTrp: 0.326 ± 0.017	0.579AsnTyr: 0.579 ± 0.023	0.0AsnXaa: 0.0 ± 0.0
Pro	6.994ProAla: 6.994 ± 0.095	0.299ProCys: 0.299 ± 0.016	4.55ProAsp: 4.55 ± 0.074	5.095ProGlu: 5.095 ± 0.071	1.853ProPhe: 1.853 ± 0.037	5.703ProGly: 5.703 ± 0.084	1.171ProHis: 1.171 ± 0.035	2.638ProIle: 2.638 ± 0.048	0.937ProLys: 0.937 ± 0.032	5.474ProLeu: 5.474 ± 0.079	1.063ProMet: 1.063 ± 0.028	1.449ProAsn: 1.449 ± 0.036	3.625ProPro: 3.625 ± 0.066	1.464ProGln: 1.464 ± 0.035	3.978ProArg: 3.978 ± 0.061	3.046ProSer: 3.046 ± 0.053	3.527ProThr: 3.527 ± 0.075	5.186ProVal: 5.186 ± 0.077	0.882ProTrp: 0.882 ± 0.033	1.429ProTyr: 1.429 ± 0.035	0.0ProXaa: 0.0 ± 0.0
Gln	4.063GlnAla: 4.063 ± 0.066	0.164GlnCys: 0.164 ± 0.012	1.364GlnAsp: 1.364 ± 0.035	2.08GlnGlu: 2.08 ± 0.047	0.923GlnPhe: 0.923 ± 0.03	2.27GlnGly: 2.27 ± 0.047	0.715GlnHis: 0.715 ± 0.024	1.789GlnIle: 1.789 ± 0.038	0.458GlnLys: 0.458 ± 0.021	2.746GlnLeu: 2.746 ± 0.047	0.74GlnMet: 0.74 ± 0.025	0.579GlnAsn: 0.579 ± 0.023	2.231GlnPro: 2.231 ± 0.046	1.389GlnGln: 1.389 ± 0.04	3.156GlnArg: 3.156 ± 0.05	1.324GlnSer: 1.324 ± 0.034	1.629GlnThr: 1.629 ± 0.042	2.923GlnVal: 2.923 ± 0.056	0.461GlnTrp: 0.461 ± 0.018	0.714GlnTyr: 0.714 ± 0.024	0.0GlnXaa: 0.0 ± 0.0
Arg	9.431ArgAla: 9.431 ± 0.108	0.579ArgCys: 0.579 ± 0.024	4.592ArgAsp: 4.592 ± 0.071	5.87ArgGlu: 5.87 ± 0.092	2.975ArgPhe: 2.975 ± 0.052	5.977ArgGly: 5.977 ± 0.073	1.856ArgHis: 1.856 ± 0.043	4.115ArgIle: 4.115 ± 0.053	1.316ArgLys: 1.316 ± 0.036	9.503ArgLeu: 9.503 ± 0.11	1.757ArgMet: 1.757 ± 0.042	1.601ArgAsn: 1.601 ± 0.039	4.525ArgPro: 4.525 ± 0.067	3.029ArgGln: 3.029 ± 0.056	8.147ArgArg: 8.147 ± 0.105	3.614ArgSer: 3.614 ± 0.071	3.969ArgThr: 3.969 ± 0.059	6.778ArgVal: 6.778 ± 0.087	1.476ArgTrp: 1.476 ± 0.041	2.441ArgTyr: 2.441 ± 0.054	0.0ArgXaa: 0.0 ± 0.0
Ser	4.825SerAla: 4.825 ± 0.059	0.275SerCys: 0.275 ± 0.014	2.529SerAsp: 2.529 ± 0.048	2.502SerGlu: 2.502 ± 0.05	1.579SerPhe: 1.579 ± 0.043	4.709SerGly: 4.709 ± 0.068	0.889SerHis: 0.889 ± 0.026	2.156SerIle: 2.156 ± 0.049	0.726SerLys: 0.726 ± 0.029	4.863SerLeu: 4.863 ± 0.076	0.988SerMet: 0.988 ± 0.028	0.994SerAsn: 0.994 ± 0.035	3.033SerPro: 3.033 ± 0.058	1.392SerGln: 1.392 ± 0.037	3.542SerArg: 3.542 ± 0.067	2.195SerSer: 2.195 ± 0.05	2.545SerThr: 2.545 ± 0.051	3.528SerVal: 3.528 ± 0.059	0.719SerTrp: 0.719 ± 0.024	1.125SerTyr: 1.125 ± 0.035	0.0SerXaa: 0.0 ± 0.0
Thr	6.33ThrAla: 6.33 ± 0.088	0.404ThrCys: 0.404 ± 0.023	2.755ThrAsp: 2.755 ± 0.057	2.917ThrGlu: 2.917 ± 0.055	1.937ThrPhe: 1.937 ± 0.046	5.384ThrGly: 5.384 ± 0.081	1.031ThrHis: 1.031 ± 0.033	3.129ThrIle: 3.129 ± 0.053	0.815ThrLys: 0.815 ± 0.029	6.286ThrLeu: 6.286 ± 0.082	1.038ThrMet: 1.038 ± 0.03	1.157ThrAsn: 1.157 ± 0.037	4.183ThrPro: 4.183 ± 0.079	1.455ThrGln: 1.455 ± 0.038	3.846ThrArg: 3.846 ± 0.065	2.582ThrSer: 2.582 ± 0.043	3.267ThrThr: 3.267 ± 0.067	5.437ThrVal: 5.437 ± 0.094	0.85ThrTrp: 0.85 ± 0.031	1.387ThrTyr: 1.387 ± 0.038	0.0ThrXaa: 0.0 ± 0.0
Val	10.706ValAla: 10.706 ± 0.108	0.589ValCys: 0.589 ± 0.021	4.981ValAsp: 4.981 ± 0.071	5.536ValGlu: 5.536 ± 0.063	2.595ValPhe: 2.595 ± 0.056	6.633ValGly: 6.633 ± 0.092	1.559ValHis: 1.559 ± 0.041	4.363ValIle: 4.363 ± 0.067	1.406ValLys: 1.406 ± 0.043	9.063ValLeu: 9.063 ± 0.107	1.606ValMet: 1.606 ± 0.041	1.817ValAsn: 1.817 ± 0.039	5.002ValPro: 5.002 ± 0.072	2.476ValGln: 2.476 ± 0.048	6.67ValArg: 6.67 ± 0.082	3.863ValSer: 3.863 ± 0.051	5.272ValThr: 5.272 ± 0.083	8.315ValVal: 8.315 ± 0.102	1.065ValTrp: 1.065 ± 0.033	1.934ValTyr: 1.934 ± 0.041	0.0ValXaa: 0.0 ± 0.0
Trp	1.298TrpAla: 1.298 ± 0.037	0.128TrpCys: 0.128 ± 0.01	0.801TrpAsp: 0.801 ± 0.029	0.946TrpGlu: 0.946 ± 0.029	0.505TrpPhe: 0.505 ± 0.02	1.025TrpGly: 1.025 ± 0.03	0.388TrpHis: 0.388 ± 0.021	0.689TrpIle: 0.689 ± 0.027	0.253TrpLys: 0.253 ± 0.017	1.873TrpLeu: 1.873 ± 0.044	0.319TrpMet: 0.319 ± 0.017	0.359TrpAsn: 0.359 ± 0.018	0.705TrpPro: 0.705 ± 0.025	0.576TrpGln: 0.576 ± 0.025	1.284TrpArg: 1.284 ± 0.037	0.798TrpSer: 0.798 ± 0.029	0.8TrpThr: 0.8 ± 0.024	1.19TrpVal: 1.19 ± 0.035	0.323TrpTrp: 0.323 ± 0.017	0.399TrpTyr: 0.399 ± 0.019	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.613TyrAla: 2.613 ± 0.048	0.232TyrCys: 0.232 ± 0.014	1.524TyrAsp: 1.524 ± 0.039	1.47TyrGlu: 1.47 ± 0.039	0.923TyrPhe: 0.923 ± 0.033	2.24TyrGly: 2.24 ± 0.044	0.645TyrHis: 0.645 ± 0.027	0.961TyrIle: 0.961 ± 0.029	0.338TyrLys: 0.338 ± 0.02	2.757TyrLeu: 2.757 ± 0.053	0.366TyrMet: 0.366 ± 0.019	0.569TyrAsn: 0.569 ± 0.026	1.464TyrPro: 1.464 ± 0.036	0.944TyrGln: 0.944 ± 0.03	2.342TyrArg: 2.342 ± 0.048	1.028TyrSer: 1.028 ± 0.033	1.29TyrThr: 1.29 ± 0.037	1.92TyrVal: 1.92 ± 0.049	0.44TyrTrp: 0.44 ± 0.02	0.697TyrTyr: 0.697 ± 0.025	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3471 proteins (1135642 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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