Wenzhou tombus-like virus 14

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 8.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KGU9|A0A1L3KGU9_9VIRU RNA-directed RNA polymerase OS=Wenzhou tombus-like virus 14 OX=1923667 PE=4 SV=1
MM1 pKa = 7.76EE2 pKa = 4.1EE3 pKa = 3.4TRR5 pKa = 11.84AKK7 pKa = 10.06IRR9 pKa = 11.84SRR11 pKa = 11.84YY12 pKa = 7.88HH13 pKa = 6.01RR14 pKa = 11.84AYY16 pKa = 9.71LTLKK20 pKa = 10.36SGKK23 pKa = 9.27KK24 pKa = 8.07WLFDD28 pKa = 3.28KK29 pKa = 11.12SLAKK33 pKa = 10.54VKK35 pKa = 10.86SFVKK39 pKa = 10.12FEE41 pKa = 4.08KK42 pKa = 10.11MSMEE46 pKa = 4.62AYY48 pKa = 10.23DD49 pKa = 3.62EE50 pKa = 4.4RR51 pKa = 11.84KK52 pKa = 8.11PARR55 pKa = 11.84LIQHH59 pKa = 6.81RR60 pKa = 11.84SYY62 pKa = 11.28EE63 pKa = 4.05YY64 pKa = 10.71LYY66 pKa = 11.04LLKK69 pKa = 10.52KK70 pKa = 11.01YY71 pKa = 7.46MFPLSKK77 pKa = 10.45ALKK80 pKa = 9.66NCTTKK85 pKa = 10.87LDD87 pKa = 3.45TGQPINTIFASGLNSKK103 pKa = 9.63QLGEE107 pKa = 5.23RR108 pKa = 11.84IHH110 pKa = 8.08QMFTQFPNCVALCLDD125 pKa = 3.31HH126 pKa = 7.11SKK128 pKa = 10.56WDD130 pKa = 3.31GHH132 pKa = 5.53FNKK135 pKa = 10.35EE136 pKa = 4.51LMEE139 pKa = 4.4LAHH142 pKa = 6.47NFWKK146 pKa = 9.45TLINHH151 pKa = 6.94LKK153 pKa = 10.29RR154 pKa = 11.84FVKK157 pKa = 9.89MVITLLVMQKK167 pKa = 10.67KK168 pKa = 10.19NYY170 pKa = 7.9ATTQHH175 pKa = 6.42LLNYY179 pKa = 9.24IFDD182 pKa = 5.69AIRR185 pKa = 11.84CSGEE189 pKa = 3.29WTTAIEE195 pKa = 4.02NSKK198 pKa = 10.73VNYY201 pKa = 8.72TILKK205 pKa = 10.25AVFPSAWILVNGDD218 pKa = 3.59DD219 pKa = 4.79SIVFIDD225 pKa = 3.29RR226 pKa = 11.84SEE228 pKa = 4.03FNKK231 pKa = 10.75LNIADD236 pKa = 3.82ILQEE240 pKa = 3.88FKK242 pKa = 10.97IFGQEE247 pKa = 3.6TKK249 pKa = 10.28LDD251 pKa = 3.65RR252 pKa = 11.84VATCMEE258 pKa = 4.99EE259 pKa = 4.94ISFCQCSPINFGEE272 pKa = 4.36YY273 pKa = 8.96YY274 pKa = 10.97KK275 pKa = 10.03MVRR278 pKa = 11.84DD279 pKa = 5.07PIRR282 pKa = 11.84AMSRR286 pKa = 11.84AAYY289 pKa = 9.1TSIEE293 pKa = 4.13MTKK296 pKa = 10.84DD297 pKa = 3.08LLPRR301 pKa = 11.84YY302 pKa = 9.79LSGLGLCEE310 pKa = 4.47LSANSGVPILQSFALHH326 pKa = 7.27LITKK330 pKa = 9.28GDD332 pKa = 3.68SSSPTTAVDD341 pKa = 4.25RR342 pKa = 11.84YY343 pKa = 9.46PYY345 pKa = 10.87AFEE348 pKa = 4.23EE349 pKa = 4.36DD350 pKa = 3.59LRR352 pKa = 11.84VIDD355 pKa = 3.63VSYY358 pKa = 8.81QTRR361 pKa = 11.84HH362 pKa = 4.83SFEE365 pKa = 4.37LAFGMSIPEE374 pKa = 3.71QLEE377 pKa = 4.02YY378 pKa = 11.22EE379 pKa = 4.4RR380 pKa = 11.84MFQHH384 pKa = 6.09NVSSSYY390 pKa = 11.07LEE392 pKa = 4.12KK393 pKa = 10.67FKK395 pKa = 10.91HH396 pKa = 4.5FHH398 pKa = 4.48QAEE401 pKa = 4.18RR402 pKa = 11.84VGWW405 pKa = 3.71

Molecular weight: 47.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KGU9|A0A1L3KGU9_9VIRU RNA-directed RNA polymerase OS=Wenzhou tombus-like virus 14 OX=1923667 PE=4 SV=1
MM1 pKa = 7.74EE2 pKa = 4.65PTLNQKK8 pKa = 7.67EE9 pKa = 4.54TVNLSASCINSGANSGGDD27 pKa = 3.43EE28 pKa = 4.19PPALKK33 pKa = 10.45RR34 pKa = 11.84PFDD37 pKa = 3.97GSGKK41 pKa = 8.77TIINEE46 pKa = 3.84STMLVNPARR55 pKa = 11.84VMYY58 pKa = 10.11NLQPNLVVHH67 pKa = 6.72PPNVQQQPVNQNQNQQPIVQQPIAQANVPPQPQAQLPAQNAQPLIPGYY115 pKa = 10.33HH116 pKa = 6.62LVPVPQPNVALHH128 pKa = 6.61NILAPTQGPQGFPNQPPPPTVVRR151 pKa = 11.84TFKK154 pKa = 10.8RR155 pKa = 11.84SWLEE159 pKa = 3.6RR160 pKa = 11.84LGEE163 pKa = 4.02LGKK166 pKa = 10.65RR167 pKa = 11.84KK168 pKa = 9.71SNKK171 pKa = 8.89YY172 pKa = 8.03PQVSAMTTVDD182 pKa = 3.49NLSGNVNRR190 pKa = 11.84LHH192 pKa = 5.54KK193 pKa = 9.48TKK195 pKa = 10.15IKK197 pKa = 10.76SRR199 pKa = 11.84FFYY202 pKa = 10.62RR203 pKa = 11.84LRR205 pKa = 11.84KK206 pKa = 9.34AATNDD211 pKa = 2.93NDD213 pKa = 4.16EE214 pKa = 4.47IMDD217 pKa = 4.3TVHH220 pKa = 7.15HH221 pKa = 6.52DD222 pKa = 3.21HH223 pKa = 7.81DD224 pKa = 4.12YY225 pKa = 11.58TMTSKK230 pKa = 9.44TFKK233 pKa = 10.88DD234 pKa = 2.72IVLRR238 pKa = 11.84NKK240 pKa = 10.32YY241 pKa = 9.9DD242 pKa = 3.34VLSNVPIEE250 pKa = 4.26EE251 pKa = 4.1AVTAVAEE258 pKa = 4.33DD259 pKa = 3.74EE260 pKa = 4.98NIPEE264 pKa = 4.09DD265 pKa = 3.57RR266 pKa = 11.84KK267 pKa = 10.83KK268 pKa = 11.13DD269 pKa = 3.68FVVALQRR276 pKa = 11.84QDD278 pKa = 3.78PNHH281 pKa = 6.73LNRR284 pKa = 11.84AVGLVRR290 pKa = 11.84LPTTSHH296 pKa = 6.0NVTTITDD303 pKa = 3.74QIDD306 pKa = 3.64IEE308 pKa = 4.74CDD310 pKa = 3.03TSEE313 pKa = 4.64LEE315 pKa = 5.13KK316 pKa = 10.63MNQRR320 pKa = 11.84SNEE323 pKa = 4.02IKK325 pKa = 9.99AHH327 pKa = 4.72TVNFTFPVKK336 pKa = 10.2RR337 pKa = 11.84KK338 pKa = 9.52KK339 pKa = 10.54LNKK342 pKa = 9.53KK343 pKa = 10.28RR344 pKa = 11.84IDD346 pKa = 3.32GTKK349 pKa = 9.37TIKK352 pKa = 10.89AFDD355 pKa = 3.43QLLWYY360 pKa = 10.43LKK362 pKa = 9.28TKK364 pKa = 10.22HH365 pKa = 6.23AFAAKK370 pKa = 8.84TLSLVTTLRR379 pKa = 11.84NDD381 pKa = 2.83ARR383 pKa = 11.84VWMATNNFKK392 pKa = 9.99MDD394 pKa = 3.43NEE396 pKa = 3.97EE397 pKa = 4.88DD398 pKa = 3.9YY399 pKa = 12.04NMMTNAVLAAMVTDD413 pKa = 4.37NKK415 pKa = 10.73DD416 pKa = 3.22LQLRR420 pKa = 11.84EE421 pKa = 3.88VLKK424 pKa = 10.79VRR426 pKa = 11.84KK427 pKa = 8.1EE428 pKa = 3.61HH429 pKa = 7.24NIRR432 pKa = 11.84HH433 pKa = 4.14VHH435 pKa = 5.2NRR437 pKa = 11.84LMLRR441 pKa = 11.84GIITKK446 pKa = 10.0KK447 pKa = 10.15FNPNVFGRR455 pKa = 11.84KK456 pKa = 5.65VWRR459 pKa = 11.84GADD462 pKa = 2.99TSFFKK467 pKa = 10.06KK468 pKa = 8.84TAISLANNVAPLL480 pKa = 3.52

Molecular weight: 54.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1373

405

488

457.7

51.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.919 ± 0.31

1.092 ± 0.354

4.516 ± 0.298

4.297 ± 0.923

4.151 ± 0.72

4.006 ± 0.395

2.331 ± 0.544

5.754 ± 0.51

7.21 ± 0.531

8.084 ± 0.826

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.768 ± 0.264

7.138 ± 1.226

6.045 ± 1.098

4.443 ± 0.596

4.443 ± 0.755

7.502 ± 1.642

8.23 ± 1.321

6.482 ± 0.891

1.238 ± 0.174

3.35 ± 0.74
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski