Aspergillus fumigatus chrysovirus
Average proteome isoelectric point is 6.36
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|D8L7M2|D8L7M2_9VIRU Uncharacterized protein OS=Aspergillus fumigatus chrysovirus OX=607716 PE=4 SV=1
MM1 pKa = 7.07 SAEE4 pKa = 4.09 LRR6 pKa = 11.84 VVDD9 pKa = 4.36 YY10 pKa = 10.25 MRR12 pKa = 11.84 GLPQSYY18 pKa = 6.63 QHH20 pKa = 6.97 RR21 pKa = 11.84 EE22 pKa = 3.39 MYY24 pKa = 8.34 YY25 pKa = 7.44 TQFMEE30 pKa = 5.24 RR31 pKa = 11.84 YY32 pKa = 9.57 NSTQLQEE39 pKa = 3.92 VDD41 pKa = 3.36 GVEE44 pKa = 4.16 VEE46 pKa = 4.49 KK47 pKa = 11.27 GLVSDD52 pKa = 4.6 GEE54 pKa = 4.3 KK55 pKa = 10.56 EE56 pKa = 4.24 IIRR59 pKa = 11.84 HH60 pKa = 5.96 LNVQDD65 pKa = 3.55 KK66 pKa = 8.61 FTMSGFRR73 pKa = 11.84 RR74 pKa = 11.84 GFRR77 pKa = 11.84 LAHH80 pKa = 6.31 MISLGQSRR88 pKa = 11.84 SVKK91 pKa = 9.38 RR92 pKa = 11.84 SKK94 pKa = 9.47 VTSLNFQGAGEE105 pKa = 4.14 WTLFASSARR114 pKa = 11.84 ALYY117 pKa = 10.11 SSKK120 pKa = 10.61 EE121 pKa = 3.84 STMNLMDD128 pKa = 4.23 EE129 pKa = 4.28 EE130 pKa = 4.29 QKK132 pKa = 9.73 RR133 pKa = 11.84 TIMSKK138 pKa = 10.61 CPTLSGSDD146 pKa = 3.14 AGGVYY151 pKa = 8.95 MAAIHH156 pKa = 6.16 EE157 pKa = 4.53 RR158 pKa = 11.84 MSTPTDD164 pKa = 3.21 LRR166 pKa = 11.84 YY167 pKa = 10.31 LFTKK171 pKa = 9.52 MVLYY175 pKa = 10.65 LYY177 pKa = 9.94 DD178 pKa = 3.32 YY179 pKa = 9.99 TLAVVSKK186 pKa = 8.95 DD187 pKa = 2.7 TGMSITEE194 pKa = 4.22 DD195 pKa = 3.42 VAFDD199 pKa = 3.68 LRR201 pKa = 11.84 ANLPEE206 pKa = 4.18 AARR209 pKa = 11.84 LATVIDD215 pKa = 3.84 HH216 pKa = 7.44 DD217 pKa = 4.19 IVIDD221 pKa = 3.93 GDD223 pKa = 4.17 LFTPEE228 pKa = 4.28 QLSLLCLSGLEE239 pKa = 4.36 YY240 pKa = 10.39 PSVWYY245 pKa = 10.31 AGEE248 pKa = 3.98 GNIYY252 pKa = 10.37 NSCCMEE258 pKa = 4.89 KK259 pKa = 10.5 DD260 pKa = 3.33 DD261 pKa = 5.55 LVIVSSGKK269 pKa = 9.79 IEE271 pKa = 3.92 VDD273 pKa = 4.0 QSFLWGSPDD282 pKa = 3.63 RR283 pKa = 11.84 MYY285 pKa = 12.1 NMMWLIAQKK294 pKa = 10.7 LNAIGCLTYY303 pKa = 10.98 ALEE306 pKa = 4.21 NMRR309 pKa = 11.84 GKK311 pKa = 10.53 CKK313 pKa = 9.94 MMSDD317 pKa = 3.04 IVAKK321 pKa = 8.08 TEE323 pKa = 3.89 CRR325 pKa = 11.84 EE326 pKa = 4.08 VNSMVPHH333 pKa = 6.77 SYY335 pKa = 11.09 CMSTAFGQIRR345 pKa = 11.84 EE346 pKa = 4.27 RR347 pKa = 11.84 QVVTKK352 pKa = 9.91 MPGFFSTSLSLISDD366 pKa = 4.84 LLYY369 pKa = 11.15 GMCFKK374 pKa = 10.9 AVASCVSEE382 pKa = 3.92 TLGAMGKK389 pKa = 9.37 VVSSSTPTTNEE400 pKa = 4.08 TINSLMRR407 pKa = 11.84 DD408 pKa = 3.38 YY409 pKa = 11.35 GLQHH413 pKa = 6.41 TEE415 pKa = 3.08 SSYY418 pKa = 11.8 NYY420 pKa = 8.27 MLHH423 pKa = 6.27 NFEE426 pKa = 5.17 LFTRR430 pKa = 11.84 KK431 pKa = 7.48 PTRR434 pKa = 11.84 WDD436 pKa = 2.72 IGQHH440 pKa = 4.01 MKK442 pKa = 10.58 EE443 pKa = 4.21 YY444 pKa = 10.47 ALKK447 pKa = 10.24 LAEE450 pKa = 4.27 DD451 pKa = 3.63 VMMGVDD457 pKa = 3.61 IPIPALLLTIPALTAVNTAFGLARR481 pKa = 11.84 GWYY484 pKa = 8.07 GQGDD488 pKa = 4.1 ILQMTKK494 pKa = 9.08 EE495 pKa = 3.97 EE496 pKa = 4.36 RR497 pKa = 11.84 LNNTDD502 pKa = 3.7 ALCAIGWMCGLRR514 pKa = 11.84 DD515 pKa = 3.42 VRR517 pKa = 11.84 PQVFRR522 pKa = 11.84 NRR524 pKa = 11.84 LGRR527 pKa = 11.84 KK528 pKa = 7.91 QLLVNSEE535 pKa = 3.86 EE536 pKa = 4.21 RR537 pKa = 11.84 KK538 pKa = 10.35 LKK540 pKa = 11.07 AEE542 pKa = 3.93 ATTDD546 pKa = 3.18 CRR548 pKa = 11.84 VRR550 pKa = 11.84 DD551 pKa = 3.55 VEE553 pKa = 4.23 FWIDD557 pKa = 3.24 EE558 pKa = 4.39 SLGGRR563 pKa = 11.84 VDD565 pKa = 4.29 EE566 pKa = 5.52 IEE568 pKa = 3.97 EE569 pKa = 4.17 AGNNLFRR576 pKa = 11.84 TEE578 pKa = 4.42 FSGTKK583 pKa = 10.0 CAMVWNYY590 pKa = 10.3 EE591 pKa = 3.64 YY592 pKa = 11.24 GMWMEE597 pKa = 4.93 AKK599 pKa = 8.84 VQEE602 pKa = 4.12 YY603 pKa = 9.05 TRR605 pKa = 11.84 LKK607 pKa = 9.98 RR608 pKa = 11.84 ASLAGDD614 pKa = 3.96 LTQRR618 pKa = 11.84 EE619 pKa = 4.55 RR620 pKa = 11.84 ATMSKK625 pKa = 9.88 IGPTPINWGPPPSHH639 pKa = 6.52 NSKK642 pKa = 10.58 LSASLEE648 pKa = 3.88 RR649 pKa = 11.84 MRR651 pKa = 11.84 AISRR655 pKa = 11.84 GNAIIPSKK663 pKa = 9.98 EE664 pKa = 4.0 PKK666 pKa = 9.39 HH667 pKa = 6.38 VRR669 pKa = 11.84 VSSDD673 pKa = 2.66 SRR675 pKa = 11.84 PVVSRR680 pKa = 11.84 YY681 pKa = 9.12 VEE683 pKa = 4.01 EE684 pKa = 5.36 GEE686 pKa = 4.62 EE687 pKa = 4.0 KK688 pKa = 10.44 DD689 pKa = 5.16 DD690 pKa = 3.55 YY691 pKa = 11.55 VPYY694 pKa = 10.52 VKK696 pKa = 10.28 PQIEE700 pKa = 4.44 EE701 pKa = 4.29 GEE703 pKa = 4.37 KK704 pKa = 9.2 ITFSEE709 pKa = 4.15 IDD711 pKa = 3.42 VPGDD715 pKa = 3.73 GSCGIHH721 pKa = 6.63 AVVKK725 pKa = 10.35 DD726 pKa = 3.78 LSIHH730 pKa = 5.9 GRR732 pKa = 11.84 IAPADD737 pKa = 3.69 AQKK740 pKa = 10.27 ATTLFSEE747 pKa = 4.75 DD748 pKa = 3.21 MASKK752 pKa = 10.31 RR753 pKa = 11.84 FHH755 pKa = 7.29 DD756 pKa = 4.18 AAEE759 pKa = 4.34 LAAQCQMWGMGMDD772 pKa = 5.56 LIDD775 pKa = 4.18 KK776 pKa = 9.5 EE777 pKa = 4.68 SNRR780 pKa = 11.84 VIRR783 pKa = 11.84 YY784 pKa = 7.95 GDD786 pKa = 3.5 PDD788 pKa = 3.6 ADD790 pKa = 3.58 YY791 pKa = 10.87 RR792 pKa = 11.84 VTIVRR797 pKa = 11.84 DD798 pKa = 3.66 GNHH801 pKa = 6.23 FKK803 pKa = 10.65 AARR806 pKa = 11.84 IGEE809 pKa = 4.55 PGHH812 pKa = 5.74 EE813 pKa = 3.9 MVVRR817 pKa = 11.84 SVEE820 pKa = 4.33 EE821 pKa = 3.84 QQSPPEE827 pKa = 3.9 EE828 pKa = 4.68 FIASVKK834 pKa = 10.81 SLGSLFGGSPIIQQ847 pKa = 3.3
Molecular weight: 95.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.41
IPC2_protein 5.461
IPC_protein 5.461
Toseland 5.614
ProMoST 5.677
Dawson 5.563
Bjellqvist 5.639
Wikipedia 5.486
Rodwell 5.512
Grimsley 5.639
Solomon 5.563
Lehninger 5.537
Nozaki 5.753
DTASelect 5.906
Thurlkill 5.766
EMBOSS 5.715
Sillero 5.842
Patrickios 4.101
IPC_peptide 5.575
IPC2_peptide 5.842
IPC2.peptide.svr19 5.836
Protein with the highest isoelectric point:
>tr|D8L7M1|D8L7M1_9VIRU Uncharacterized protein OS=Aspergillus fumigatus chrysovirus OX=607716 PE=4 SV=1
MM1 pKa = 7.87 AMATYY6 pKa = 9.78 KK7 pKa = 10.92 GSGGGAYY14 pKa = 10.37 GPMDD18 pKa = 4.35 LRR20 pKa = 11.84 RR21 pKa = 11.84 QVTEE25 pKa = 3.53 QKK27 pKa = 10.15 RR28 pKa = 11.84 DD29 pKa = 3.24 VFRR32 pKa = 11.84 EE33 pKa = 3.8 LRR35 pKa = 11.84 RR36 pKa = 11.84 EE37 pKa = 3.83 AVALRR42 pKa = 11.84 KK43 pKa = 9.52 AVEE46 pKa = 4.49 LRR48 pKa = 11.84 NSMDD52 pKa = 2.65 VWDD55 pKa = 4.12 PSTRR59 pKa = 11.84 YY60 pKa = 8.81 GAKK63 pKa = 8.99 TVRR66 pKa = 11.84 EE67 pKa = 3.82 KK68 pKa = 10.97 SDD70 pKa = 3.11 RR71 pKa = 11.84 FGRR74 pKa = 11.84 DD75 pKa = 2.38 IGSLLAYY82 pKa = 9.47 FEE84 pKa = 4.97 NVHH87 pKa = 6.46 SPALDD92 pKa = 3.51 VIARR96 pKa = 11.84 DD97 pKa = 3.55 RR98 pKa = 11.84 FMLRR102 pKa = 11.84 YY103 pKa = 9.32 QIHH106 pKa = 6.54 GDD108 pKa = 3.2 IDD110 pKa = 3.85 RR111 pKa = 11.84 DD112 pKa = 3.7 AVIGANTMTVTVPVHH127 pKa = 5.16 WEE129 pKa = 3.85 KK130 pKa = 11.48 LDD132 pKa = 3.62 VTVEE136 pKa = 4.36 LYY138 pKa = 9.96 PGPLDD143 pKa = 4.15 KK144 pKa = 11.28 LMSQTKK150 pKa = 10.52 LEE152 pKa = 4.12 GFARR156 pKa = 11.84 SGIPDD161 pKa = 3.56 RR162 pKa = 11.84 DD163 pKa = 3.27 QVAKK167 pKa = 9.05 HH168 pKa = 5.67 TGWNKK173 pKa = 9.92 DD174 pKa = 3.3 DD175 pKa = 3.6 VRR177 pKa = 11.84 RR178 pKa = 11.84 FPDD181 pKa = 3.05 DD182 pKa = 3.97 RR183 pKa = 11.84 MYY185 pKa = 11.52 LLQLLLEE192 pKa = 4.22 QMQTGQSKK200 pKa = 8.36 LTRR203 pKa = 11.84 LVKK206 pKa = 10.65 GFLLMLEE213 pKa = 4.26 VAEE216 pKa = 5.01 RR217 pKa = 11.84 GPTDD221 pKa = 3.84 FDD223 pKa = 4.08 VGNHH227 pKa = 4.74 THH229 pKa = 5.22 VQYY232 pKa = 11.49 APAAVINSFEE242 pKa = 4.19 QPDD245 pKa = 3.19 RR246 pKa = 11.84 CYY248 pKa = 11.22 VYY250 pKa = 10.62 NSKK253 pKa = 9.79 PAQIAHH259 pKa = 6.28 TALLLAMCRR268 pKa = 11.84 AYY270 pKa = 9.85 PPPNFSSHH278 pKa = 4.49 ITIPADD284 pKa = 3.42 APQVCLVSQGQAPNPGGAVTLTAGLIYY311 pKa = 10.53 SSLVTYY317 pKa = 11.22 AMDD320 pKa = 4.09 TSCTGLLQQAQIIACSLQEE339 pKa = 3.54 NRR341 pKa = 11.84 YY342 pKa = 7.97 FSKK345 pKa = 10.97 VGLPQVASLYY355 pKa = 11.15 DD356 pKa = 3.45 LMIPAFVAHH365 pKa = 6.75 SSSLQNAMLSKK376 pKa = 10.9 DD377 pKa = 3.32 LAKK380 pKa = 10.66 SVGRR384 pKa = 11.84 VHH386 pKa = 6.89 QMLGFVSAKK395 pKa = 10.53 DD396 pKa = 3.67 VIQASTMQATQGFDD410 pKa = 2.86 VSATVRR416 pKa = 11.84 QYY418 pKa = 11.82 LNSNATLVHH427 pKa = 5.8 QMASKK432 pKa = 10.84 LSGIGIFDD440 pKa = 3.91 ATPKK444 pKa = 10.62 LRR446 pKa = 11.84 VFDD449 pKa = 4.2 YY450 pKa = 10.53 MDD452 pKa = 3.32 TRR454 pKa = 11.84 DD455 pKa = 4.31 YY456 pKa = 11.76 SDD458 pKa = 5.01 LLNLSLFEE466 pKa = 4.21 GLWLVRR472 pKa = 11.84 NATSSVHH479 pKa = 5.76 NGPVGFLLNGEE490 pKa = 4.44 RR491 pKa = 11.84 LRR493 pKa = 11.84 TADD496 pKa = 3.11 KK497 pKa = 10.18 TGYY500 pKa = 10.09 DD501 pKa = 3.51 VLVEE505 pKa = 4.08 EE506 pKa = 5.0 LVLAGVTVEE515 pKa = 3.95 HH516 pKa = 6.84 HH517 pKa = 6.48 KK518 pKa = 10.77 MPDD521 pKa = 3.23 GAFTTSWVGVLRR533 pKa = 11.84 HH534 pKa = 6.45 DD535 pKa = 4.57 PLRR538 pKa = 11.84 QSRR541 pKa = 11.84 RR542 pKa = 11.84 PRR544 pKa = 11.84 RR545 pKa = 11.84 RR546 pKa = 11.84 IEE548 pKa = 3.8 TTLVRR553 pKa = 11.84 EE554 pKa = 4.31 CDD556 pKa = 3.81 YY557 pKa = 11.14 KK558 pKa = 11.34 PEE560 pKa = 4.16 MNLKK564 pKa = 10.11 AGGRR568 pKa = 11.84 KK569 pKa = 7.8 VRR571 pKa = 11.84 KK572 pKa = 9.02 AVRR575 pKa = 11.84 RR576 pKa = 11.84 PTRR579 pKa = 11.84 EE580 pKa = 3.83 VPSAVPLSQILKK592 pKa = 7.98 TPSPQRR598 pKa = 11.84 QRR600 pKa = 11.84 SVRR603 pKa = 11.84 RR604 pKa = 11.84 EE605 pKa = 4.01 SPPQYY610 pKa = 10.49 QSDD613 pKa = 4.19 FSSSPVTEE621 pKa = 4.45 DD622 pKa = 3.11 QSGEE626 pKa = 4.09 ALSQQASEE634 pKa = 4.23 EE635 pKa = 4.22 DD636 pKa = 3.88 LFDD639 pKa = 4.24 EE640 pKa = 5.28 EE641 pKa = 5.06 PPLHH645 pKa = 4.98 QTQVARR651 pKa = 11.84 EE652 pKa = 4.09 AQRR655 pKa = 11.84 KK656 pKa = 7.8 VGRR659 pKa = 11.84 STLEE663 pKa = 4.16 RR664 pKa = 11.84 ILEE667 pKa = 4.19 AVEE670 pKa = 5.13 EE671 pKa = 4.15 VDD673 pKa = 3.6 KK674 pKa = 11.69 DD675 pKa = 3.69 KK676 pKa = 11.73 AEE678 pKa = 4.15 EE679 pKa = 3.77 QAKK682 pKa = 9.08 VNRR685 pKa = 11.84 RR686 pKa = 11.84 IEE688 pKa = 4.2 SFILKK693 pKa = 8.95 TSPQFNPRR701 pKa = 11.84 EE702 pKa = 3.87 VTMRR706 pKa = 11.84 ICRR709 pKa = 11.84 EE710 pKa = 3.63 GGLSVRR716 pKa = 11.84 EE717 pKa = 3.83 RR718 pKa = 11.84 EE719 pKa = 3.96 EE720 pKa = 3.35 WLEE723 pKa = 3.77 AVEE726 pKa = 4.88 RR727 pKa = 11.84 SIGTAKK733 pKa = 10.61 ASRR736 pKa = 11.84 ARR738 pKa = 11.84 DD739 pKa = 3.59 LRR741 pKa = 11.84 EE742 pKa = 3.87 LADD745 pKa = 3.42 MLVAHH750 pKa = 7.11 GLRR753 pKa = 11.84 GATRR757 pKa = 11.84 RR758 pKa = 11.84 LSGNHH763 pKa = 7.07 KK764 pKa = 8.29 YY765 pKa = 8.97 TKK767 pKa = 10.0 LVNSAIEE774 pKa = 4.3 SNNPSLANDD783 pKa = 4.83 LLTSMRR789 pKa = 11.84 VQGSAWEE796 pKa = 4.29 GDD798 pKa = 4.1 VIAAPQKK805 pKa = 10.09 GYY807 pKa = 11.11 KK808 pKa = 10.27 FIANSPAWSEE818 pKa = 3.83 ALQDD822 pKa = 3.4 QGINPDD828 pKa = 3.35 VLVANRR834 pKa = 11.84 PMRR837 pKa = 11.84 YY838 pKa = 9.48 VLADD842 pKa = 4.22 FLADD846 pKa = 3.32 STTFTAQAIGRR857 pKa = 11.84 LGAWLDD863 pKa = 3.75 GCSVNLLPHH872 pKa = 6.68 RR873 pKa = 11.84 LKK875 pKa = 10.93 EE876 pKa = 4.12 DD877 pKa = 3.62 VIPAQIPPEE886 pKa = 4.22 LDD888 pKa = 2.65 WYY890 pKa = 10.97 RR891 pKa = 11.84 NPTSMEE897 pKa = 3.2 ARR899 pKa = 11.84 KK900 pKa = 9.41 FNKK903 pKa = 9.58 EE904 pKa = 3.62 DD905 pKa = 3.8 SRR907 pKa = 11.84 WLYY910 pKa = 10.59 LAARR914 pKa = 11.84 LPGGVYY920 pKa = 9.54 TKK922 pKa = 11.03 EE923 pKa = 3.91 EE924 pKa = 4.33 LVPLCAEE931 pKa = 3.6 FRR933 pKa = 11.84 VPVEE937 pKa = 3.83 LRR939 pKa = 11.84 NQLRR943 pKa = 11.84 VKK945 pKa = 10.42 YY946 pKa = 10.2 GLFNRR951 pKa = 11.84 EE952 pKa = 3.59 EE953 pKa = 4.04
Molecular weight: 106.81 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.448
IPC2_protein 7.849
IPC_protein 7.717
Toseland 7.6
ProMoST 8.244
Dawson 8.478
Bjellqvist 8.682
Wikipedia 8.448
Rodwell 8.507
Grimsley 7.658
Solomon 8.551
Lehninger 8.551
Nozaki 8.785
DTASelect 8.478
Thurlkill 8.551
EMBOSS 8.653
Sillero 8.814
Patrickios 4.66
IPC_peptide 8.536
IPC2_peptide 7.512
IPC2.peptide.svr19 7.747
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
3805
847
1114
951.3
107.58
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.07 ± 0.497
1.445 ± 0.149
5.966 ± 0.087
6.965 ± 0.281
3.311 ± 0.755
6.833 ± 0.563
2.181 ± 0.135
4.494 ± 0.393
4.731 ± 0.146
9.382 ± 0.725
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.233 ± 0.423
3.601 ± 0.242
3.916 ± 0.434
3.89 ± 0.308
7.543 ± 0.51
7.306 ± 0.44
4.888 ± 0.22
7.937 ± 0.365
1.813 ± 0.234
3.495 ± 0.315
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here