Soybean vein necrosis virus
Average proteome isoelectric point is 6.63
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|F2Y3N6|F2Y3N6_9VIRU Non-structural protein OS=Soybean vein necrosis virus OX=980895 PE=4 SV=1
MM1 pKa = 7.91 RR2 pKa = 11.84 IKK4 pKa = 10.86 DD5 pKa = 3.61 FFVIMLLFGLNLVFLSIFINASIRR29 pKa = 11.84 GDD31 pKa = 3.46 HH32 pKa = 6.39 EE33 pKa = 4.65 VSQEE37 pKa = 3.89 TSFEE41 pKa = 4.11 EE42 pKa = 4.07 LSKK45 pKa = 11.17 EE46 pKa = 3.91 VMEE49 pKa = 4.29 AHH51 pKa = 6.03 EE52 pKa = 4.09 QGKK55 pKa = 9.67 IGKK58 pKa = 8.54 SLVSSRR64 pKa = 11.84 VEE66 pKa = 3.64 AFEE69 pKa = 4.45 SFNKK73 pKa = 10.15 EE74 pKa = 3.73 PIEE77 pKa = 3.94 TSRR80 pKa = 11.84 RR81 pKa = 11.84 PEE83 pKa = 3.67 MNDD86 pKa = 4.02 RR87 pKa = 11.84 ITQTKK92 pKa = 9.63 PGIDD96 pKa = 3.43 DD97 pKa = 3.44 QMIPKK102 pKa = 9.76 HH103 pKa = 6.25 GEE105 pKa = 4.16 GKK107 pKa = 10.16 RR108 pKa = 11.84 EE109 pKa = 3.94 APPPSKK115 pKa = 11.0 VDD117 pKa = 3.74 LSCANINPEE126 pKa = 3.68 NCKK129 pKa = 10.34 VVGKK133 pKa = 8.49 TPFNFYY139 pKa = 10.59 YY140 pKa = 10.31 QISDD144 pKa = 3.68 SEE146 pKa = 4.33 EE147 pKa = 4.25 TISCLSSKK155 pKa = 10.82 VEE157 pKa = 4.57 DD158 pKa = 4.35 LKK160 pKa = 11.51 ACNPQSMHH168 pKa = 5.85 VFTSAPVLPITTILNKK184 pKa = 10.04 RR185 pKa = 11.84 VLSVGSKK192 pKa = 10.64 YY193 pKa = 10.83 YY194 pKa = 9.78 IQNSIDD200 pKa = 3.44 SSSFPVQDD208 pKa = 3.37 SGNVQLGLVNIYY220 pKa = 8.46 SVRR223 pKa = 11.84 LTGEE227 pKa = 3.98 CNITKK232 pKa = 10.03 VSLTNPFFVTLTSSNKK248 pKa = 6.99 EE249 pKa = 3.8 TFYY252 pKa = 10.94 SIKK255 pKa = 10.48 KK256 pKa = 9.94 IGADD260 pKa = 3.53 DD261 pKa = 3.72 VNLIKK266 pKa = 10.76 FSGEE270 pKa = 3.81 KK271 pKa = 9.39 TVEE274 pKa = 4.07 LPSDD278 pKa = 3.87 SVDD281 pKa = 3.43 GNHH284 pKa = 6.49 VLLCGDD290 pKa = 4.17 KK291 pKa = 10.82 VSEE294 pKa = 4.15 IPKK297 pKa = 9.43 IDD299 pKa = 3.56 VNKK302 pKa = 10.28 RR303 pKa = 11.84 NCLVKK308 pKa = 10.93 YY309 pKa = 9.93 KK310 pKa = 10.54 GKK312 pKa = 10.46 RR313 pKa = 11.84 YY314 pKa = 8.06 QQSACVHH321 pKa = 5.66 FNILRR326 pKa = 11.84 YY327 pKa = 9.69 LLVSIILFVPVSWFLNKK344 pKa = 9.97 TKK346 pKa = 11.0 DD347 pKa = 5.21 SMFLWYY353 pKa = 10.0 DD354 pKa = 3.01 FLGIIFYY361 pKa = 9.9 PILYY365 pKa = 9.1 ILNWLWRR372 pKa = 11.84 YY373 pKa = 9.47 FPMKK377 pKa = 10.32 CSNCGNFSLITHH389 pKa = 6.32 SCYY392 pKa = 9.73 DD393 pKa = 3.3 TCVCNKK399 pKa = 9.9 SKK401 pKa = 10.93 AKK403 pKa = 10.35 SEE405 pKa = 4.03 HH406 pKa = 6.74 AEE408 pKa = 3.75 NCPIITGKK416 pKa = 10.48 AFSKK420 pKa = 11.07 SEE422 pKa = 3.97 DD423 pKa = 3.45 QDD425 pKa = 3.7 VDD427 pKa = 3.82 DD428 pKa = 5.73 LSVEE432 pKa = 4.24 EE433 pKa = 4.69 TEE435 pKa = 5.08 SLTGSKK441 pKa = 10.06 KK442 pKa = 9.79 PKK444 pKa = 9.23 KK445 pKa = 9.86 ASSKK449 pKa = 10.05 FRR451 pKa = 11.84 TFQLVVNTKK460 pKa = 9.66 ISTGFLMFVTKK471 pKa = 10.65 VLLGFLIFSQMPKK484 pKa = 9.75 TMASKK489 pKa = 8.98 TATQTISQTEE499 pKa = 4.24 TQVLCVSYY507 pKa = 10.81 CSAKK511 pKa = 10.06 PGCNKK516 pKa = 9.72 FLFKK520 pKa = 11.1 NEE522 pKa = 4.06 EE523 pKa = 3.96 VCIDD527 pKa = 3.85 NPKK530 pKa = 10.34 LKK532 pKa = 10.31 CNCLIRR538 pKa = 11.84 DD539 pKa = 3.55 GMINEE544 pKa = 4.01 EE545 pKa = 4.22 AYY547 pKa = 10.03 INGTRR552 pKa = 11.84 YY553 pKa = 9.72 SVSMVDD559 pKa = 2.89 NCLDD563 pKa = 3.54 RR564 pKa = 11.84 RR565 pKa = 11.84 CEE567 pKa = 3.76 FSSNKK572 pKa = 9.48 VEE574 pKa = 5.25 DD575 pKa = 4.26 LLACRR580 pKa = 11.84 LGCGQLKK587 pKa = 10.08 KK588 pKa = 10.74 LKK590 pKa = 10.26 SSKK593 pKa = 10.26 LQSKK597 pKa = 9.54 ALKK600 pKa = 10.08 QGIVPRR606 pKa = 11.84 GASISQIYY614 pKa = 10.31 SSLRR618 pKa = 11.84 LQDD621 pKa = 4.11 GYY623 pKa = 10.78 IDD625 pKa = 4.09 SEE627 pKa = 4.45 EE628 pKa = 4.29 SLHH631 pKa = 7.34 SLGDD635 pKa = 3.6 LPEE638 pKa = 4.31 NKK640 pKa = 9.71 VKK642 pKa = 10.56 IEE644 pKa = 4.67 DD645 pKa = 3.48 EE646 pKa = 4.84 DD647 pKa = 3.86 IPEE650 pKa = 4.44 GVLPRR655 pKa = 11.84 QSFVYY660 pKa = 10.59 NSIVDD665 pKa = 3.57 GKK667 pKa = 9.86 YY668 pKa = 9.85 RR669 pKa = 11.84 YY670 pKa = 9.25 LISMDD675 pKa = 4.97 AIQSTGYY682 pKa = 9.78 VYY684 pKa = 11.43 SMNDD688 pKa = 2.79 AKK690 pKa = 10.31 MAPPQEE696 pKa = 3.72 LVVFVKK702 pKa = 10.49 EE703 pKa = 4.04 AGVTYY708 pKa = 9.9 TMKK711 pKa = 10.01 TLYY714 pKa = 8.81 YY715 pKa = 8.02 TAPISATHH723 pKa = 4.55 THH725 pKa = 6.12 VYY727 pKa = 7.95 STCTGNCEE735 pKa = 4.35 TCRR738 pKa = 11.84 KK739 pKa = 7.05 EE740 pKa = 4.37 HH741 pKa = 7.07 PISGYY746 pKa = 8.75 QDD748 pKa = 3.24 YY749 pKa = 11.11 CIAPTSNWGCEE760 pKa = 4.03 EE761 pKa = 4.63 LGCLAIGEE769 pKa = 4.64 GATCGYY775 pKa = 9.0 CRR777 pKa = 11.84 NVYY780 pKa = 10.1 DD781 pKa = 4.27 LSKK784 pKa = 10.35 KK785 pKa = 9.69 YY786 pKa = 10.14 SVRR789 pKa = 11.84 QILTSHH795 pKa = 5.46 VTVKK799 pKa = 10.58 VCFKK803 pKa = 10.59 GFAGTGCQTITDD815 pKa = 4.06 SVPFQNSYY823 pKa = 10.06 YY824 pKa = 10.03 QLSIDD829 pKa = 4.63 ADD831 pKa = 3.84 LHH833 pKa = 7.16 NDD835 pKa = 3.45 DD836 pKa = 4.57 LTAGSRR842 pKa = 11.84 VAVDD846 pKa = 3.5 PNGMLFKK853 pKa = 11.33 GNIANLKK860 pKa = 10.15 DD861 pKa = 3.19 SSLAFGHH868 pKa = 6.23 PQLNEE873 pKa = 3.84 LGVLMFAKK881 pKa = 10.62 ANLEE885 pKa = 3.95 LSDD888 pKa = 4.14 FTWSCAVIGNKK899 pKa = 9.78 KK900 pKa = 10.19 VDD902 pKa = 3.8 VKK904 pKa = 11.15 KK905 pKa = 10.73 CGYY908 pKa = 8.68 DD909 pKa = 3.07 TFHH912 pKa = 7.33 QYY914 pKa = 10.06 IGLEE918 pKa = 4.29 PISDD922 pKa = 3.92 YY923 pKa = 10.98 FADD926 pKa = 5.01 EE927 pKa = 4.41 IDD929 pKa = 4.31 DD930 pKa = 3.46 GTMFVKK936 pKa = 10.42 KK937 pKa = 10.05 DD938 pKa = 3.65 FKK940 pKa = 10.53 IGKK943 pKa = 9.2 INLIVDD949 pKa = 4.46 LPSEE953 pKa = 4.52 LFQTIAKK960 pKa = 8.25 KK961 pKa = 10.48 PKK963 pKa = 7.99 VAMVASACKK972 pKa = 10.39 GCLSCSSGINCDD984 pKa = 4.28 LEE986 pKa = 4.43 FTSDD990 pKa = 3.84 SIFSCRR996 pKa = 11.84 ISWEE1000 pKa = 4.12 HH1001 pKa = 6.14 CTSEE1005 pKa = 4.19 PEE1007 pKa = 3.91 QIAMKK1012 pKa = 10.47 KK1013 pKa = 7.53 GTNVFKK1019 pKa = 11.07 LKK1021 pKa = 10.39 PCFALKK1027 pKa = 10.15 IQLLQKK1033 pKa = 10.34 IILVPSDD1040 pKa = 4.14 DD1041 pKa = 3.52 EE1042 pKa = 5.24 SMALEE1047 pKa = 4.21 FEE1049 pKa = 4.59 TKK1051 pKa = 10.15 NVEE1054 pKa = 3.68 ISDD1057 pKa = 4.01 PEE1059 pKa = 4.3 TIIDD1063 pKa = 4.07 HH1064 pKa = 6.9 NDD1066 pKa = 3.07 DD1067 pKa = 3.68 AFNEE1071 pKa = 4.19 EE1072 pKa = 4.09 VEE1074 pKa = 4.3 YY1075 pKa = 11.1 DD1076 pKa = 3.2 SDD1078 pKa = 3.9 TSFKK1082 pKa = 11.11 SVWDD1086 pKa = 4.14 YY1087 pKa = 11.46 IKK1089 pKa = 11.26 SPFNWVASFFGDD1101 pKa = 3.39 FFEE1104 pKa = 4.37 IVRR1107 pKa = 11.84 VLLVLTCCAVLFISISKK1124 pKa = 10.18 LYY1126 pKa = 8.94 EE1127 pKa = 3.77 ICHH1130 pKa = 6.35 EE1131 pKa = 4.42 YY1132 pKa = 10.83 YY1133 pKa = 10.69 SQEE1136 pKa = 3.98 QYY1138 pKa = 10.99 KK1139 pKa = 10.42 KK1140 pKa = 10.49 SSRR1143 pKa = 11.84 KK1144 pKa = 9.6 KK1145 pKa = 10.66 EE1146 pKa = 3.74 GDD1148 pKa = 4.18 LEE1150 pKa = 6.33 DD1151 pKa = 5.81 DD1152 pKa = 4.71 DD1153 pKa = 4.91 EE1154 pKa = 6.36 KK1155 pKa = 11.17 KK1156 pKa = 10.97 AQDD1159 pKa = 4.98 DD1160 pKa = 4.13 IIKK1163 pKa = 10.32 SLRR1166 pKa = 11.84 NQVTEE1171 pKa = 3.65 DD1172 pKa = 3.09 TTYY1175 pKa = 11.1 KK1176 pKa = 10.53 RR1177 pKa = 11.84 NSNKK1181 pKa = 8.24 STRR1184 pKa = 11.84 KK1185 pKa = 10.15 APFDD1189 pKa = 3.75 DD1190 pKa = 5.22 LIIRR1194 pKa = 11.84 VV1195 pKa = 3.71
Molecular weight: 134.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.539
IPC2_protein 5.677
IPC_protein 5.69
Toseland 5.83
ProMoST 5.995
Dawson 5.893
Bjellqvist 5.944
Wikipedia 5.868
Rodwell 5.868
Grimsley 5.868
Solomon 5.893
Lehninger 5.881
Nozaki 6.135
DTASelect 6.313
Thurlkill 6.262
EMBOSS 6.224
Sillero 6.237
Patrickios 3.846
IPC_peptide 5.906
IPC2_peptide 6.186
IPC2.peptide.svr19 6.272
Protein with the highest isoelectric point:
>tr|F2Y3N7|F2Y3N7_9VIRU Nucleocapsid protein OS=Soybean vein necrosis virus OX=980895 PE=3 SV=1
MM1 pKa = 7.51 PQTAGPSNAKK11 pKa = 8.57 PAKK14 pKa = 8.76 ITEE17 pKa = 4.3 SNLAKK22 pKa = 10.46 LLKK25 pKa = 10.25 FEE27 pKa = 4.68 EE28 pKa = 5.13 DD29 pKa = 3.57 IEE31 pKa = 4.35 FEE33 pKa = 4.3 KK34 pKa = 11.19 NSTGFKK40 pKa = 10.25 FSEE43 pKa = 5.09 FYY45 pKa = 8.4 KK46 pKa = 9.31 THH48 pKa = 6.22 MGRR51 pKa = 11.84 KK52 pKa = 8.72 FRR54 pKa = 11.84 YY55 pKa = 9.21 ASALTFLKK63 pKa = 10.41 NRR65 pKa = 11.84 KK66 pKa = 9.6 AIVNMCKK73 pKa = 10.24 KK74 pKa = 9.82 GTFNFDD80 pKa = 2.95 GQTVKK85 pKa = 10.99 LSVEE89 pKa = 4.25 SGDD92 pKa = 4.25 DD93 pKa = 3.3 NSFTFKK99 pKa = 10.92 RR100 pKa = 11.84 LDD102 pKa = 3.09 SFLRR106 pKa = 11.84 VKK108 pKa = 10.19 MLEE111 pKa = 3.95 HH112 pKa = 6.46 NFAVFDD118 pKa = 4.0 GTNEE122 pKa = 3.77 EE123 pKa = 4.71 AKK125 pKa = 10.55 QSLCNDD131 pKa = 4.09 LATIPLVQAYY141 pKa = 9.83 GLTVKK146 pKa = 10.8 DD147 pKa = 3.56 KK148 pKa = 10.61 MSAKK152 pKa = 9.6 LAIMIGGSLPLLASITGCEE171 pKa = 4.16 AYY173 pKa = 10.67 CFGLAIFQDD182 pKa = 3.99 LKK184 pKa = 11.05 KK185 pKa = 9.96 EE186 pKa = 3.77 QLGIVNFDD194 pKa = 3.66 TKK196 pKa = 11.15 AQAAKK201 pKa = 10.03 VASVLDD207 pKa = 3.64 AKK209 pKa = 10.71 GFKK212 pKa = 9.03 FTEE215 pKa = 4.54 EE216 pKa = 3.95 KK217 pKa = 10.84 NQTLRR222 pKa = 11.84 LIAEE226 pKa = 4.42 ILKK229 pKa = 11.01 DD230 pKa = 3.41 MAPQMRR236 pKa = 11.84 GVASLEE242 pKa = 4.21 KK243 pKa = 10.21 YY244 pKa = 10.27 NEE246 pKa = 4.03 QIGIISDD253 pKa = 3.93 IIGVHH258 pKa = 6.18 FEE260 pKa = 4.12 MPGKK264 pKa = 10.31 KK265 pKa = 9.66 DD266 pKa = 3.53 GKK268 pKa = 9.79 GKK270 pKa = 10.11 KK271 pKa = 9.74 SKK273 pKa = 10.26 EE274 pKa = 3.77 FSVV277 pKa = 3.57
Molecular weight: 30.75 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.575
IPC2_protein 8.361
IPC_protein 8.244
Toseland 9.209
ProMoST 8.843
Dawson 9.399
Bjellqvist 9.136
Wikipedia 9.487
Rodwell 9.911
Grimsley 9.399
Solomon 9.472
Lehninger 9.458
Nozaki 9.414
DTASelect 9.048
Thurlkill 9.297
EMBOSS 9.619
Sillero 9.414
Patrickios 5.715
IPC_peptide 9.472
IPC2_peptide 7.629
IPC2.peptide.svr19 7.846
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
5159
277
2931
1031.8
117.36
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.09 ± 0.677
2.384 ± 0.5
5.99 ± 0.328
6.978 ± 0.395
5.02 ± 0.508
5.117 ± 0.667
1.667 ± 0.209
7.269 ± 0.237
9.304 ± 0.545
9.033 ± 0.666
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.198 ± 0.451
5.582 ± 0.341
3.024 ± 0.422
2.811 ± 0.265
3.353 ± 0.391
9.343 ± 0.324
5.679 ± 0.5
5.408 ± 0.5
0.678 ± 0.165
4.071 ± 0.546
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here