Tai Forest hepadnavirus
Average proteome isoelectric point is 8.89
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A482KMS0|A0A482KMS0_9HEPA Core protein OS=Tai Forest hepadnavirus OX=2557875 PE=4 SV=1
MM1 pKa = 7.44 GNIASEE7 pKa = 4.2 SLAPLFGLQVGYY19 pKa = 9.03 FLWTKK24 pKa = 9.88 ILSIAQSLDD33 pKa = 3.36 SWWTSLSFPGGIPRR47 pKa = 11.84 CAGLNSPFPTCDD59 pKa = 3.59 HH60 pKa = 6.91 SPTSCPVTCTGYY72 pKa = 10.69 RR73 pKa = 11.84 WMCLRR78 pKa = 11.84 RR79 pKa = 11.84 FIIYY83 pKa = 10.41 LLVLVLCLIFLLVLLDD99 pKa = 4.11 CKK101 pKa = 10.96 GLIPVCPIGPSAGTRR116 pKa = 11.84 GVSCRR121 pKa = 11.84 TCTQPVDD128 pKa = 3.44 EE129 pKa = 5.93 GIWIPSCCCSKK140 pKa = 10.88 SLEE143 pKa = 4.35 GNCTCLAIPSSWALGRR159 pKa = 11.84 YY160 pKa = 8.53 LWGLASARR168 pKa = 11.84 FSWLSSLQPWLQWFAGLSPIVWLLLIWMMWYY199 pKa = 9.19 WGPGLLSILTPFIPLLLLFFWISVSLL225 pKa = 3.76
Molecular weight: 25.02 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.439
IPC2_protein 7.293
IPC_protein 7.117
Toseland 6.491
ProMoST 7.732
Dawson 7.819
Bjellqvist 8.375
Wikipedia 7.702
Rodwell 7.834
Grimsley 6.44
Solomon 7.849
Lehninger 7.878
Nozaki 8.77
DTASelect 7.98
Thurlkill 8.024
EMBOSS 8.024
Sillero 8.434
Patrickios 0.21
IPC_peptide 7.849
IPC2_peptide 8.302
IPC2.peptide.svr19 8.364
Protein with the highest isoelectric point:
>tr|A0A482KIF7|A0A482KIF7_9HEPA Surface protein OS=Tai Forest hepadnavirus OX=2557875 PE=4 SV=1
MM1 pKa = 7.74 AARR4 pKa = 11.84 LRR6 pKa = 11.84 CRR8 pKa = 11.84 LDD10 pKa = 3.05 PSRR13 pKa = 11.84 NILHH17 pKa = 6.72 LRR19 pKa = 11.84 PVGPQSSGRR28 pKa = 11.84 SISRR32 pKa = 11.84 SSRR35 pKa = 11.84 LSPLPAAAALRR46 pKa = 11.84 SFHH49 pKa = 6.84 RR50 pKa = 11.84 SHH52 pKa = 6.65 VPLRR56 pKa = 11.84 SLPSCAWSPAGPCVLRR72 pKa = 11.84 FTCADD77 pKa = 3.31 LRR79 pKa = 11.84 RR80 pKa = 11.84 CMGAPMNFARR90 pKa = 11.84 WHH92 pKa = 5.19 GRR94 pKa = 11.84 RR95 pKa = 11.84 MRR97 pKa = 11.84 GLTVGTLNRR106 pKa = 11.84 WQDD109 pKa = 3.73 FYY111 pKa = 11.59 HH112 pKa = 6.55 GLFMQLWEE120 pKa = 4.2 DD121 pKa = 3.66 QGWSEE126 pKa = 4.4 RR127 pKa = 11.84 LCTYY131 pKa = 10.89 VLGGCRR137 pKa = 11.84 HH138 pKa = 6.08 KK139 pKa = 10.88 LVPTLL144 pKa = 3.79
Molecular weight: 16.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.146
IPC2_protein 9.94
IPC_protein 11.389
Toseland 11.506
ProMoST 12.003
Dawson 11.52
Bjellqvist 11.506
Wikipedia 11.989
Rodwell 11.096
Grimsley 11.55
Solomon 12.003
Lehninger 11.901
Nozaki 11.52
DTASelect 11.506
Thurlkill 11.506
EMBOSS 12.003
Sillero 11.506
Patrickios 10.847
IPC_peptide 12.003
IPC2_peptide 11.008
IPC2.peptide.svr19 9.677
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
1408
144
819
352.0
39.69
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.895 ± 0.486
3.338 ± 1.209
3.409 ± 0.658
2.841 ± 0.65
4.901 ± 0.393
6.463 ± 0.659
2.983 ± 0.682
3.693 ± 1.14
3.125 ± 1.125
13.636 ± 1.025
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.634 ± 0.396
3.196 ± 0.69
8.097 ± 0.269
2.983 ± 0.294
7.599 ± 1.6
8.949 ± 0.708
6.25 ± 0.771
5.185 ± 0.366
3.338 ± 0.981
2.486 ± 0.451
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here