Erysipelothrix rhusiopathiae ATCC 19414

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Erysipelotrichia; Erysipelotrichales; Erysipelotrichaceae; Erysipelothrix; Erysipelothrix rhusiopathiae

Average proteome isoelectric point is 6.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1644 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E7FU42|E7FU42_ERYRH Uncharacterized protein OS=Erysipelothrix rhusiopathiae ATCC 19414 OX=525280 GN=HMPREF0357_10148 PE=4 SV=1
MM1 pKa = 7.59EE2 pKa = 5.07EE3 pKa = 4.13KK4 pKa = 10.72SLIVINDD11 pKa = 3.78DD12 pKa = 3.45GTEE15 pKa = 3.79QEE17 pKa = 3.97MDD19 pKa = 3.05ILFTFDD25 pKa = 5.52DD26 pKa = 3.7DD27 pKa = 4.43TFNKK31 pKa = 10.2KK32 pKa = 9.72YY33 pKa = 10.55VLYY36 pKa = 10.53VSPEE40 pKa = 3.95DD41 pKa = 3.49TTGEE45 pKa = 4.36VFVSSYY51 pKa = 11.56VEE53 pKa = 4.87DD54 pKa = 3.76GTLNAVTDD62 pKa = 4.13PKK64 pKa = 9.41EE65 pKa = 3.47WAMIEE70 pKa = 4.16EE71 pKa = 4.36VFNAFVIQHH80 pKa = 6.67DD81 pKa = 3.95EE82 pKa = 4.29SNEE85 pKa = 3.83EE86 pKa = 4.07GACACGKK93 pKa = 9.82DD94 pKa = 3.73HH95 pKa = 7.25EE96 pKa = 4.85SSEE99 pKa = 4.49DD100 pKa = 3.62HH101 pKa = 6.71EE102 pKa = 4.92CSCGCGHH109 pKa = 7.01DD110 pKa = 3.9HH111 pKa = 6.67SHH113 pKa = 6.72

Molecular weight: 12.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E7FX53|E7FX53_ERYRH Uncharacterized protein OS=Erysipelothrix rhusiopathiae ATCC 19414 OX=525280 GN=HMPREF0357_10808 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.14QPSKK9 pKa = 9.95RR10 pKa = 11.84KK11 pKa = 7.81HH12 pKa = 5.11QKK14 pKa = 8.48VHH16 pKa = 5.53GFRR19 pKa = 11.84ARR21 pKa = 11.84MKK23 pKa = 8.28TVGGRR28 pKa = 11.84RR29 pKa = 11.84VLSRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84SKK37 pKa = 10.63GRR39 pKa = 11.84KK40 pKa = 8.44VLSAA44 pKa = 4.05

Molecular weight: 5.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1644

0

1644

526108

41

2167

320.0

36.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.237 ± 0.059

0.807 ± 0.02

6.01 ± 0.06

6.917 ± 0.066

4.597 ± 0.054

6.29 ± 0.059

2.139 ± 0.027

8.24 ± 0.06

6.62 ± 0.06

9.582 ± 0.074

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.942 ± 0.033

5.0 ± 0.05

3.165 ± 0.035

3.503 ± 0.033

4.009 ± 0.044

6.315 ± 0.046

5.568 ± 0.05

7.317 ± 0.054

0.733 ± 0.02

4.005 ± 0.044
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski