Paenibacillus sp. JCM 10914

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus; unclassified Paenibacillus

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6080 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|V9G3T4|V9G3T4_9BACL Diaminopimelate epimerase OS=Paenibacillus sp. JCM 10914 OX=1236974 GN=dapF PE=3 SV=1
MM1 pKa = 7.62VNIQISPSTSSQFEE15 pKa = 3.89PNIAVNWLNPSVMVAVAVDD34 pKa = 4.12LRR36 pKa = 11.84LGPPNIGLYY45 pKa = 10.44KK46 pKa = 10.66SIDD49 pKa = 3.52AGATWSTTILPIPTGFAGIEE69 pKa = 4.13APHH72 pKa = 7.25IDD74 pKa = 4.1YY75 pKa = 10.66IFPNTFVVLAHH86 pKa = 6.84AFDD89 pKa = 4.36SDD91 pKa = 3.92GLSGSIVSYY100 pKa = 9.93TSIDD104 pKa = 3.65NASSFAPPVIVNQGFGQFVNDD125 pKa = 3.89DD126 pKa = 3.69QVVVVTDD133 pKa = 3.87KK134 pKa = 11.41AGSSPFFGNVYY145 pKa = 9.58TGYY148 pKa = 7.65THH150 pKa = 8.11DD151 pKa = 4.95YY152 pKa = 7.71NTQFIPGNTIFFNRR166 pKa = 11.84SLDD169 pKa = 3.43SGNTYY174 pKa = 10.07EE175 pKa = 5.31SPVLLSSVQEE185 pKa = 4.07FEE187 pKa = 4.43EE188 pKa = 4.89FPGIAITLTGIVVVGWINQPPGNTEE213 pKa = 3.85LNIRR217 pKa = 11.84TSPDD221 pKa = 2.79GGVTFTTEE229 pKa = 3.81TMVDD233 pKa = 3.64SVVIPPDD240 pKa = 4.14PLPGYY245 pKa = 7.47QFRR248 pKa = 11.84CLTYY252 pKa = 9.68PSLAADD258 pKa = 4.03ISNAPTSRR266 pKa = 11.84GNVYY270 pKa = 10.57AVWQDD275 pKa = 3.26FRR277 pKa = 11.84EE278 pKa = 5.26GYY280 pKa = 10.42SDD282 pKa = 3.46IFMATSTNSGIDD294 pKa = 2.85WGTPISITGSPVGSQSFFPVITVSPADD321 pKa = 3.31GAIFVTYY328 pKa = 7.63YY329 pKa = 9.45TNRR332 pKa = 11.84VNPLNIDD339 pKa = 3.43VFLATSRR346 pKa = 11.84DD347 pKa = 3.45GGLTFTNTRR356 pKa = 11.84ITTTSFDD363 pKa = 3.52VTGLLLIGDD372 pKa = 4.56YY373 pKa = 10.2IGNAIVPQTARR384 pKa = 11.84LVSVWTDD391 pKa = 2.74TRR393 pKa = 11.84TGTEE397 pKa = 4.28NIWFGDD403 pKa = 3.68NQQ405 pKa = 3.46

Molecular weight: 43.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|V9G8K7|V9G8K7_9BACL Heme efflux system ATPase HrtA OS=Paenibacillus sp. JCM 10914 OX=1236974 GN=JCM10914_2345 PE=4 SV=1
MM1 pKa = 7.9RR2 pKa = 11.84PTFKK6 pKa = 10.82PNVSKK11 pKa = 10.8RR12 pKa = 11.84KK13 pKa = 8.95KK14 pKa = 8.25VHH16 pKa = 5.49GFRR19 pKa = 11.84KK20 pKa = 10.01RR21 pKa = 11.84MSTKK25 pKa = 10.18NGRR28 pKa = 11.84KK29 pKa = 8.78VLANRR34 pKa = 11.84RR35 pKa = 11.84QKK37 pKa = 10.09GRR39 pKa = 11.84KK40 pKa = 8.54VLSAA44 pKa = 4.05

Molecular weight: 5.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6080

0

6080

1730134

37

3264

284.6

31.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.82 ± 0.03

0.738 ± 0.01

5.223 ± 0.026

6.801 ± 0.034

4.034 ± 0.026

7.383 ± 0.041

2.296 ± 0.016

6.872 ± 0.029

4.855 ± 0.023

10.016 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.056 ± 0.013

3.839 ± 0.022

4.074 ± 0.021

4.099 ± 0.023

5.142 ± 0.027

6.412 ± 0.024

5.441 ± 0.034

7.002 ± 0.031

1.338 ± 0.016

3.559 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski