Sodalis praecaptivus
Average proteome isoelectric point is 6.79
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4281 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|W0HPK6|W0HPK6_9GAMM Flagellar secretion chaperone FliS OS=Sodalis praecaptivus OX=1239307 GN=fliS2 PE=3 SV=1
MM1 pKa = 7.53 GFSQAVSGLNAASSNLDD18 pKa = 3.36 VIGNNIANAEE28 pKa = 4.12 TSGFKK33 pKa = 10.4 SGSVSFADD41 pKa = 3.57 VFSSSKK47 pKa = 10.56 VGLGVKK53 pKa = 9.34 VASVTQNFNDD63 pKa = 4.16 GTVSTTDD70 pKa = 2.89 NGLDD74 pKa = 3.37 VAISNNGFFRR84 pKa = 11.84 LVDD87 pKa = 3.74 GSGAVYY93 pKa = 10.16 YY94 pKa = 10.67 SRR96 pKa = 11.84 NGEE99 pKa = 3.83 FSLNADD105 pKa = 3.74 RR106 pKa = 11.84 QLVNSSGYY114 pKa = 10.47 VVTGYY119 pKa = 10.53 AASGNPATIQQGADD133 pKa = 3.44 PVALTIPSQGLSASATTSGSMVMSLTSSATSVGTTAFDD171 pKa = 4.63 PNDD174 pKa = 3.21 SSTYY178 pKa = 10.71 SFSQPLTTYY187 pKa = 11.0 DD188 pKa = 3.99 SLGNPHH194 pKa = 6.48 NVSLYY199 pKa = 9.62 FAKK202 pKa = 10.22 TEE204 pKa = 4.33 DD205 pKa = 4.03 NTWEE209 pKa = 4.26 VYY211 pKa = 10.62 SVDD214 pKa = 3.4 NSNGDD219 pKa = 3.37 GTVNDD224 pKa = 3.71 VGTLNFDD231 pKa = 3.76 TNGQLTGTDD240 pKa = 3.08 TFSINMAALNGAPAQTFNLSFEE262 pKa = 4.51 GTRR265 pKa = 11.84 QQYY268 pKa = 10.8 ASSSSVSSQTQDD280 pKa = 2.74 GYY282 pKa = 11.5 SAGDD286 pKa = 3.47 LTSYY290 pKa = 9.52 TINDD294 pKa = 4.22 DD295 pKa = 3.62 GTISGTYY302 pKa = 10.51 SNGKK306 pKa = 6.7 TQLLGQIVLANFANPQGLQSDD327 pKa = 4.63 GNNLWSATSASGQAIVGTAGSGTFGTLTSGALEE360 pKa = 4.15 SSNVDD365 pKa = 3.63 LSQEE369 pKa = 4.1 LVNMIVAQRR378 pKa = 11.84 NYY380 pKa = 10.23 QSNAQTIKK388 pKa = 9.16 TQDD391 pKa = 4.02 AILQTLVSLRR401 pKa = 3.71
Molecular weight: 41.3 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.72
IPC2_protein 3.821
IPC_protein 3.846
Toseland 3.605
ProMoST 4.024
Dawson 3.846
Bjellqvist 4.012
Wikipedia 3.821
Rodwell 3.668
Grimsley 3.516
Solomon 3.846
Lehninger 3.795
Nozaki 3.961
DTASelect 4.266
Thurlkill 3.668
EMBOSS 3.821
Sillero 3.973
Patrickios 1.914
IPC_peptide 3.834
IPC2_peptide 3.935
IPC2.peptide.svr19 3.86
Protein with the highest isoelectric point:
>tr|W0HRQ2|W0HRQ2_9GAMM 2Fe-2S ferredoxin OS=Sodalis praecaptivus OX=1239307 GN=yfaE PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.68 RR12 pKa = 11.84 NRR14 pKa = 11.84 THH16 pKa = 7.28 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.4 NGRR28 pKa = 11.84 QVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.36 GRR39 pKa = 11.84 TRR41 pKa = 11.84 LTVSSKK47 pKa = 11.13
Molecular weight: 5.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.676
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.398
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.177
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4281
0
4281
1400054
16
1965
327.0
35.92
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.129 ± 0.045
1.091 ± 0.012
5.195 ± 0.027
5.013 ± 0.036
3.639 ± 0.024
7.703 ± 0.035
2.371 ± 0.017
5.47 ± 0.028
3.341 ± 0.03
11.356 ± 0.054
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.574 ± 0.016
3.26 ± 0.025
4.912 ± 0.032
4.517 ± 0.032
6.531 ± 0.034
5.648 ± 0.025
5.228 ± 0.023
6.886 ± 0.031
1.4 ± 0.016
2.737 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here