Proteome-pI 2.0 - Proteome Isoelectric Point Database

Baja California bark scorpion polyomavirus 1

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cossaviricota; Papovaviricetes; Sepolyvirales; Polyomaviridae; unclassified Polyomaviridae

Average proteome isoelectric point is 7.62

Get precalculated fractions of proteins

Acidic

pI < 6.8

6.8-7.4

pI > 7.4

Basic

All

Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions

Protein with the lowest isoelectric point:

>tr|A0A161K1S9|A0A161K1S9_9POLY VP1e OS=Baja California bark scorpion polyomavirus 1 OX=1648839 PE=4 SV=1

MM1 pKa = 7.35GGVVSTVGAVVLFLIEE17 pKa = 4.1EE18 pKa = 4.19FAEE21 pKa = 4.29TGAIAGSAAAVGAGEE36 pKa = 4.59LSVEE40 pKa = 4.32SAAAGALGDD49 pKa = 5.0AIALAAEE56 pKa = 4.07EE57 pKa = 4.61EE58 pKa = 4.4IVIFEE63 pKa = 4.29STSAAVSGGTYY74 pKa = 10.19GSLYY78 pKa = 10.41SGSALTSGLDD88 pKa = 3.62LLADD92 pKa = 3.74TAIGEE97 pKa = 4.52SASAAALDD105 pKa = 3.95SEE107 pKa = 4.55NALGAFFANQSINIGAATSVSVGSAIGLTAWIVANGYY144 pKa = 10.16DD145 pKa = 3.7YY146 pKa = 11.53SLEE149 pKa = 4.11QVDD152 pKa = 4.62KK153 pKa = 10.95LVPTIDD159 pKa = 3.52QVGSGLFMNLYY170 pKa = 9.52FRR172 pKa = 11.84KK173 pKa = 9.81KK174 pKa = 9.41KK175 pKa = 9.95KK176 pKa = 9.52KK177 pKa = 8.24RR178 pKa = 11.84HH179 pKa = 5.66AGSEE183 pKa = 3.84EE184 pKa = 3.8GMLPKK189 pKa = 10.33LYY191 pKa = 10.06KK192 pKa = 9.77RR193 pKa = 11.84KK194 pKa = 9.84KK195 pKa = 7.28VQSYY199 pKa = 10.0

Molecular weight: 20.14 kDa
Isoelectric point according different methods:

Protein with the highest isoelectric point:

>tr|A0A160ST56|A0A160ST56_9POLY VP1 OS=Baja California bark scorpion polyomavirus 1 OX=1648839 PE=4 SV=1

MM1 pKa = 7.42SALLIQRR8 pKa = 11.84SWPGYY13 pKa = 6.21TEE15 pKa = 4.53KK16 pKa = 11.0NADD19 pKa = 3.02NFIRR23 pKa = 11.84TRR25 pKa = 11.84AVPKK29 pKa = 10.28KK30 pKa = 9.48KK31 pKa = 9.81PNYY34 pKa = 9.87SIPLSPNIKK43 pKa = 10.0NLNRR47 pKa = 11.84TQDD50 pKa = 3.18AAFSAKK56 pKa = 9.57KK57 pKa = 10.67SLMTGSNLRR66 pKa = 11.84VRR68 pKa = 11.84LRR70 pKa = 11.84LKK72 pKa = 10.52KK73 pKa = 9.69ILKK76 pKa = 9.6FRR78 pKa = 11.84IFII81 pKa = 4.35

Molecular weight: 9.38 kDa
Isoelectric point according different methods:

Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try ESI

ChTry ESI

ArgC ESI

LysN ESI

TryLysC ESI

Try MALDI

ChTry MALDI

ArgC MALDI

LysN MALDI

TryLysC MALDI

Try LTQ

ChTry LTQ

ArgC LTQ

LysN LTQ

TryLysC LTQ

Try MSlow

ChTry MSlow

ArgC MSlow

LysN MSlow

TryLysC MSlow

Try MShigh

ChTry MShigh

ArgC MShigh

LysN MShigh

TryLysC MShigh

General Statistics

Number of major isoforms	Number of additional isoforms	Number of all proteins	Number of amino acids	Min. Seq. Length	Max. Seq. Length	Avg. Seq. Length	Avg. Mol. Weight
6	0	6	1862	81	725	310.3	35.38

Amino acid frequency

Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu
6.337 ± 1.671	3.222 ± 0.803	5.156 ± 1.003	5.854 ± 0.661	4.78 ± 0.69	5.263 ± 1.234	2.256 ± 0.614	6.606 ± 0.556	8.324 ± 0.808	7.68 ± 0.998

Met	Asn	Gln	Pro	Arg	Ser	Thr	Val	Trp	Tyr
2.417 ± 0.239	6.23 ± 0.818	4.834 ± 1.054	3.008 ± 0.499	5.424 ± 0.803	6.767 ± 1.169	5.263 ± 0.737	5.371 ± 0.579	1.343 ± 0.465	3.867 ± 0.272

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here

See this proteome in:

Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski