Amphiamblys sp. WSBS2006

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Fungi incertae sedis; Microsporidia; Microsporidia incertae sedis; Amphiacanthidae; Amphiamblys; unclassified Amphiamblys

Average proteome isoelectric point is 6.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3608 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1J5WU15|A0A1J5WU15_9MICR CMGC/CDK protein kinase (Fragment) OS=Amphiamblys sp. WSBS2006 OX=1866961 GN=A8A55_2827 PE=4 SV=1
DDD2 pKa = 3.72VGEEE6 pKa = 4.14EE7 pKa = 3.91SEEE10 pKa = 4.29VQTTIEEE17 pKa = 4.09DDD19 pKa = 3.44KKK21 pKa = 9.22EE22 pKa = 3.79VLNITNIYYY31 pKa = 8.01TGTIYYY37 pKa = 9.0TNVITSQTTYYY48 pKa = 11.2VVTTSSVEEE57 pKa = 3.93TDDD60 pKa = 3.92VATTTSLIEEE70 pKa = 3.76DDD72 pKa = 3.45TIVSTSVVQGTTNVTATVITNIYYY96 pKa = 9.5HH97 pKa = 6.06TTLTEEE103 pKa = 4.39RR104 pKa = 11.84QSTYYY109 pKa = 10.85YY110 pKa = 8.89TEEE113 pKa = 4.14VYYY116 pKa = 9.41TITEEE121 pKa = 4.37PDDD124 pKa = 3.09EE125 pKa = 4.35EE126 pKa = 4.45

Molecular weight: 13.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1J5WH42|A0A1J5WH42_9MICR Uncharacterized protein OS=Amphiamblys sp. WSBS2006 OX=1866961 GN=A8A55_2056 PE=4 SV=1
MM1 pKa = 6.75TQVVWVTRR9 pKa = 11.84QEE11 pKa = 4.14MKK13 pKa = 10.46KK14 pKa = 9.84QALNRR19 pKa = 11.84PQHH22 pKa = 5.22QALNRR27 pKa = 11.84PQQQALNRR35 pKa = 11.84TLNRR39 pKa = 11.84PQHH42 pKa = 5.2QALNRR47 pKa = 11.84PQQQALNRR55 pKa = 11.84TLNRR59 pKa = 11.84TQQQALNRR67 pKa = 11.84PQQQAINRR75 pKa = 11.84TLNRR79 pKa = 11.84TQQQALNRR87 pKa = 11.84PQQQAINRR95 pKa = 11.84TQNQAINRR103 pKa = 11.84TQHH106 pKa = 4.46QAINRR111 pKa = 11.84TQNQAINRR119 pKa = 11.84TQHH122 pKa = 5.05QAINQSWQQVHH133 pKa = 5.34SS134 pKa = 4.05

Molecular weight: 15.89 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3608

0

3608

1182352

51

4326

327.7

37.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.636 ± 0.042

2.102 ± 0.019

4.615 ± 0.022

9.643 ± 0.059

4.415 ± 0.034

6.388 ± 0.038

2.115 ± 0.016

5.507 ± 0.036

7.838 ± 0.054

9.708 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.122 ± 0.015

3.367 ± 0.023

3.506 ± 0.028

2.928 ± 0.025

6.479 ± 0.037

6.514 ± 0.03

5.657 ± 0.042

6.97 ± 0.035

0.996 ± 0.013

2.489 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski