Tomato leaf curl Cameroon virus - [Cameroon:Buea:Okra:2008]
Average proteome isoelectric point is 7.61
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|C0MHQ4|C0MHQ4_9GEMI Uncharacterized protein C4 OS=Tomato leaf curl Cameroon virus - [Cameroon:Buea:Okra:2008] OX=693895 GN=C4 PE=3 SV=1
MM1 pKa = 7.6 APPRR5 pKa = 11.84 KK6 pKa = 9.35 FRR8 pKa = 11.84 INSKK12 pKa = 10.68 NYY14 pKa = 8.18 FLTYY18 pKa = 9.21 PKK20 pKa = 10.44 CSLEE24 pKa = 4.03 KK25 pKa = 10.99 EE26 pKa = 4.25 EE27 pKa = 6.15 ALSQLQNLDD36 pKa = 3.17 TPTNKK41 pKa = 10.07 KK42 pKa = 9.7 YY43 pKa = 10.84 IKK45 pKa = 9.66 ICRR48 pKa = 11.84 EE49 pKa = 3.53 LHH51 pKa = 6.26 EE52 pKa = 4.93 NGEE55 pKa = 4.25 PHH57 pKa = 6.94 LHH59 pKa = 6.18 VLLQFEE65 pKa = 4.96 GKK67 pKa = 10.14 YY68 pKa = 10.06 NCQNQRR74 pKa = 11.84 FFDD77 pKa = 4.04 LVSPTRR83 pKa = 11.84 SAHH86 pKa = 4.86 FHH88 pKa = 6.55 PNIQGAKK95 pKa = 9.25 SSSDD99 pKa = 3.11 VKK101 pKa = 11.24 SYY103 pKa = 10.79 IDD105 pKa = 3.7 KK106 pKa = 11.33 DD107 pKa = 3.49 GDD109 pKa = 3.67 TLEE112 pKa = 4.14 WGEE115 pKa = 3.84 FQIDD119 pKa = 3.21 GRR121 pKa = 11.84 SARR124 pKa = 11.84 GGCQNANDD132 pKa = 4.15 AAAEE136 pKa = 3.91 ALNAGSAEE144 pKa = 3.76 AAMAIIKK151 pKa = 9.57 EE152 pKa = 4.1 KK153 pKa = 10.64 LPKK156 pKa = 10.32 EE157 pKa = 4.83 FIFQYY162 pKa = 11.09 HH163 pKa = 5.35 NLKK166 pKa = 10.92 SNLDD170 pKa = 4.04 RR171 pKa = 11.84 IFQEE175 pKa = 4.24 PPAPYY180 pKa = 9.99 VSPFLSSSFNQVPEE194 pKa = 4.2 EE195 pKa = 3.99 LEE197 pKa = 3.93 EE198 pKa = 4.22 WVSEE202 pKa = 4.09 NVMSAAARR210 pKa = 11.84 PLRR213 pKa = 11.84 PISIVIEE220 pKa = 4.23 GDD222 pKa = 3.09 SRR224 pKa = 11.84 TGKK227 pKa = 7.65 TVWARR232 pKa = 11.84 SLGPHH237 pKa = 6.71 NYY239 pKa = 10.18 LCGHH243 pKa = 7.38 LDD245 pKa = 4.18 LSPKK249 pKa = 10.08 VYY251 pKa = 10.98 SNNAWYY257 pKa = 10.49 NVIDD261 pKa = 5.17 DD262 pKa = 4.41 VDD264 pKa = 3.61 PHH266 pKa = 5.85 YY267 pKa = 11.0 LKK269 pKa = 10.7 HH270 pKa = 6.2 FKK272 pKa = 10.7 EE273 pKa = 4.46 FMGAQRR279 pKa = 11.84 DD280 pKa = 3.81 WQSNTKK286 pKa = 9.26 YY287 pKa = 10.63 GKK289 pKa = 9.49 PIQIKK294 pKa = 10.42 GGIPTIFLCNPGPTSSYY311 pKa = 10.69 IEE313 pKa = 4.09 YY314 pKa = 10.67 LDD316 pKa = 3.84 EE317 pKa = 4.59 EE318 pKa = 4.54 KK319 pKa = 11.06 NAALKK324 pKa = 10.42 AWAIKK329 pKa = 10.28 NATFFTLNEE338 pKa = 4.11 PLYY341 pKa = 10.87 SSADD345 pKa = 3.54 QSPAPHH351 pKa = 6.69 RR352 pKa = 11.84 EE353 pKa = 4.17 EE354 pKa = 4.44 EE355 pKa = 4.23 SSEE358 pKa = 3.97 AA359 pKa = 3.83
Molecular weight: 40.61 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.646
IPC2_protein 5.639
IPC_protein 5.664
Toseland 6.046
ProMoST 5.995
Dawson 5.893
Bjellqvist 5.919
Wikipedia 5.893
Rodwell 5.881
Grimsley 6.198
Solomon 5.893
Lehninger 5.893
Nozaki 6.148
DTASelect 6.338
Thurlkill 6.338
EMBOSS 6.287
Sillero 6.249
Patrickios 4.037
IPC_peptide 5.919
IPC2_peptide 6.237
IPC2.peptide.svr19 6.262
Protein with the highest isoelectric point:
>tr|C0MHQ1|C0MHQ1_9GEMI Replication enhancer OS=Tomato leaf curl Cameroon virus - [Cameroon:Buea:Okra:2008] OX=693895 GN=C3 PE=3 SV=1
MM1 pKa = 7.7 SKK3 pKa = 10.44 RR4 pKa = 11.84 PGDD7 pKa = 3.95 IIISTPVSKK16 pKa = 10.56 VRR18 pKa = 11.84 RR19 pKa = 11.84 RR20 pKa = 11.84 LNFDD24 pKa = 3.04 SPYY27 pKa = 8.82 TSRR30 pKa = 11.84 AVVPTVRR37 pKa = 11.84 GISKK41 pKa = 9.84 RR42 pKa = 11.84 RR43 pKa = 11.84 SWTYY47 pKa = 9.79 RR48 pKa = 11.84 PMYY51 pKa = 9.54 RR52 pKa = 11.84 KK53 pKa = 8.92 PRR55 pKa = 11.84 MYY57 pKa = 10.84 RR58 pKa = 11.84 MYY60 pKa = 10.6 KK61 pKa = 10.21 SPDD64 pKa = 3.18 VPRR67 pKa = 11.84 GCEE70 pKa = 4.4 GPCKK74 pKa = 9.92 IQSFEE79 pKa = 3.75 QRR81 pKa = 11.84 DD82 pKa = 3.89 DD83 pKa = 3.73 VKK85 pKa = 10.8 HH86 pKa = 6.08 VGIVRR91 pKa = 11.84 CVSDD95 pKa = 3.32 VTRR98 pKa = 11.84 GSGLTHH104 pKa = 6.46 RR105 pKa = 11.84 VGKK108 pKa = 9.86 RR109 pKa = 11.84 FCIKK113 pKa = 10.24 SICILGKK120 pKa = 9.74 VWMDD124 pKa = 3.5 EE125 pKa = 4.15 NIKK128 pKa = 10.08 KK129 pKa = 9.99 QNHH132 pKa = 5.07 TNNVMFFLVRR142 pKa = 11.84 DD143 pKa = 3.66 RR144 pKa = 11.84 RR145 pKa = 11.84 PRR147 pKa = 3.65
Molecular weight: 17.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.303
IPC2_protein 9.897
IPC_protein 10.935
Toseland 11.096
ProMoST 11.272
Dawson 11.14
Bjellqvist 10.95
Wikipedia 11.433
Rodwell 11.257
Grimsley 11.184
Solomon 11.403
Lehninger 11.345
Nozaki 11.082
DTASelect 10.935
Thurlkill 11.082
EMBOSS 11.52
Sillero 11.096
Patrickios 10.965
IPC_peptide 11.403
IPC2_peptide 10.248
IPC2.peptide.svr19 8.741
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
2
9
1274
85
359
141.6
16.17
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.73 ± 1.024
2.355 ± 0.396
4.474 ± 0.328
5.181 ± 0.924
4.239 ± 0.4
4.71 ± 0.565
4.16 ± 0.521
6.358 ± 0.859
5.573 ± 0.668
7.692 ± 0.815
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.884 ± 0.465
5.887 ± 0.61
5.573 ± 0.735
4.474 ± 0.557
6.515 ± 1.216
8.006 ± 0.82
6.436 ± 1.227
5.887 ± 0.896
1.334 ± 0.179
3.532 ± 0.574
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here