Nostoc phage N1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 89 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A191SAU8|A0A191SAU8_9CAUD Uncharacterized protein OS=Nostoc phage N1 OX=1775255 PE=4 SV=1
MM1 pKa = 7.77SLEE4 pKa = 4.08LCYY7 pKa = 10.79QRR9 pKa = 11.84ITQTDD14 pKa = 3.6RR15 pKa = 11.84VNGQLVDD22 pKa = 3.63TVSYY26 pKa = 10.36IKK28 pKa = 10.8VFAGMLTRR36 pKa = 11.84VEE38 pKa = 4.4YY39 pKa = 11.13NFTDD43 pKa = 4.5FGTPDD48 pKa = 3.24DD49 pKa = 4.21WQPGEE54 pKa = 4.27NDD56 pKa = 3.18LYY58 pKa = 9.7MYY60 pKa = 11.0SMPGDD65 pKa = 3.67DD66 pKa = 3.63NVYY69 pKa = 10.72NVPCYY74 pKa = 9.95ISQRR78 pKa = 11.84SVTYY82 pKa = 9.87EE83 pKa = 3.63NGVLVSTNTKK93 pKa = 9.52YY94 pKa = 10.9INGAADD100 pKa = 3.23RR101 pKa = 11.84VFITEE106 pKa = 4.35FVIQSEE112 pKa = 4.5NEE114 pKa = 3.77MMPAGWSPTPGVDD127 pKa = 3.23KK128 pKa = 10.74ILYY131 pKa = 9.07EE132 pKa = 4.16DD133 pKa = 4.01VYY135 pKa = 11.57DD136 pKa = 4.09VNEE139 pKa = 3.94EE140 pKa = 4.02RR141 pKa = 5.12

Molecular weight: 16.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A191SAT6|A0A191SAT6_9CAUD Uncharacterized protein OS=Nostoc phage N1 OX=1775255 PE=4 SV=1
MM1 pKa = 7.14KK2 pKa = 10.0TPGKK6 pKa = 9.02TGFVKK11 pKa = 9.81TARR14 pKa = 11.84KK15 pKa = 9.74DD16 pKa = 3.18GKK18 pKa = 7.48TTVNRR23 pKa = 11.84LSKK26 pKa = 10.43IMKK29 pKa = 9.75LSNLFASLSACAIAVSSFASPVMAQRR55 pKa = 11.84PYY57 pKa = 10.27IVSVPGKK64 pKa = 7.35TAEE67 pKa = 4.24GDD69 pKa = 3.58TLKK72 pKa = 11.0YY73 pKa = 10.83DD74 pKa = 3.53VTSLDD79 pKa = 3.28NHH81 pKa = 6.24RR82 pKa = 11.84ADD84 pKa = 3.69YY85 pKa = 11.74VNFTYY90 pKa = 10.82YY91 pKa = 10.58VISRR95 pKa = 11.84TGDD98 pKa = 2.98LRR100 pKa = 11.84QNRR103 pKa = 11.84GWTACGVRR111 pKa = 11.84DD112 pKa = 3.53GWLVTRR118 pKa = 11.84SDD120 pKa = 5.3GSTAWINVGSTASVNMLNSVCSRR143 pKa = 11.84VFPHH147 pKa = 6.12WRR149 pKa = 11.84IKK151 pKa = 10.58

Molecular weight: 16.6 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

89

0

89

18376

49

912

206.5

23.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.676 ± 0.305

1.034 ± 0.113

5.937 ± 0.214

5.861 ± 0.339

4.849 ± 0.212

6.079 ± 0.293

1.594 ± 0.157

8.185 ± 0.245

6.987 ± 0.377

8.631 ± 0.225

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.073 ± 0.138

6.236 ± 0.195

3.82 ± 0.184

3.973 ± 0.176

3.945 ± 0.186

7.221 ± 0.272

6.313 ± 0.327

6.274 ± 0.254

1.137 ± 0.09

4.174 ± 0.177
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski