Merida-like virus KE-2017a

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Rhabdoviridae; unclassified Rhabdoviridae

Average proteome isoelectric point is 7.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1U9YAZ4|A0A1U9YAZ4_9RHAB GDP polyribonucleotidyltransferase OS=Merida-like virus KE-2017a OX=1969830 PE=4 SV=1
MM1 pKa = 7.61SEE3 pKa = 4.06PGGEE7 pKa = 4.16SVQTTKK13 pKa = 10.7SEE15 pKa = 4.03EE16 pKa = 4.07EE17 pKa = 3.53IRR19 pKa = 11.84AEE21 pKa = 3.9SFYY24 pKa = 11.35SVGSDD29 pKa = 3.56LPQEE33 pKa = 4.43SVSCLSALLEE43 pKa = 3.99YY44 pKa = 10.67DD45 pKa = 3.67QEE47 pKa = 4.41GLEE50 pKa = 4.33KK51 pKa = 10.2TFSQIDD57 pKa = 3.85LEE59 pKa = 4.82DD60 pKa = 4.38PLTGEE65 pKa = 4.46GGGGSVQVAVPWGKK79 pKa = 9.77IPEE82 pKa = 4.27EE83 pKa = 4.06KK84 pKa = 9.6QAEE87 pKa = 4.27YY88 pKa = 11.06LKK90 pKa = 11.01LLSSYY95 pKa = 10.26NIPPAEE101 pKa = 4.2TSDD104 pKa = 3.75EE105 pKa = 4.13EE106 pKa = 5.44SGVDD110 pKa = 4.75DD111 pKa = 5.37DD112 pKa = 4.88KK113 pKa = 11.7QSSGTPSLPPTSPSLPSEE131 pKa = 4.1RR132 pKa = 11.84GNRR135 pKa = 11.84PLSPFLFSDD144 pKa = 4.03EE145 pKa = 4.67EE146 pKa = 4.11YY147 pKa = 10.94SEE149 pKa = 4.37YY150 pKa = 11.31VDD152 pKa = 3.36IFVPEE157 pKa = 4.07VLQDD161 pKa = 3.39VSLRR165 pKa = 11.84TPLMSLLRR173 pKa = 11.84DD174 pKa = 3.7VVRR177 pKa = 11.84VMVNSKK183 pKa = 10.66NEE185 pKa = 4.05PLYY188 pKa = 10.98SLQKK192 pKa = 9.19YY193 pKa = 8.31DD194 pKa = 3.75LRR196 pKa = 11.84EE197 pKa = 3.99GTVRR201 pKa = 11.84LARR204 pKa = 11.84VGHH207 pKa = 7.06LFTYY211 pKa = 10.17CPEE214 pKa = 4.21GTPIPVLEE222 pKa = 4.27QRR224 pKa = 11.84GSPKK228 pKa = 9.61PQKK231 pKa = 10.28KK232 pKa = 10.48GIFATTQKK240 pKa = 11.14DD241 pKa = 3.25LTAPSVRR248 pKa = 11.84EE249 pKa = 3.89TTSRR253 pKa = 11.84GVTADD258 pKa = 5.14PIPSGSKK265 pKa = 8.79TLADD269 pKa = 3.69SASGQINQVSQTFEE283 pKa = 4.19EE284 pKa = 4.64TWPSLEE290 pKa = 4.66HH291 pKa = 6.65SGSPLNLEE299 pKa = 3.97RR300 pKa = 11.84GVNVQRR306 pKa = 11.84AVSPSSSSHH315 pKa = 5.3YY316 pKa = 10.24SQTSSVEE323 pKa = 4.27SNSDD327 pKa = 3.32NEE329 pKa = 4.14EE330 pKa = 4.17EE331 pKa = 4.21IEE333 pKa = 4.5VMQEE337 pKa = 4.17TYY339 pKa = 11.46SFMCPIYY346 pKa = 10.19GSSEE350 pKa = 3.75MSVKK354 pKa = 10.01VFPGYY359 pKa = 9.73EE360 pKa = 3.95VEE362 pKa = 4.63PLLKK366 pKa = 10.64LGLCPPEE373 pKa = 3.86IFRR376 pKa = 11.84SILRR380 pKa = 11.84DD381 pKa = 3.37RR382 pKa = 11.84GLLRR386 pKa = 11.84ALEE389 pKa = 4.2AVLDD393 pKa = 3.7IGQTHH398 pKa = 5.3MTT400 pKa = 3.99

Molecular weight: 43.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1U9YAZ7|A0A1U9YAZ7_9RHAB Nucleocapsid protein OS=Merida-like virus KE-2017a OX=1969830 PE=4 SV=1
MM1 pKa = 7.41SFVSMLKK8 pKa = 10.52GKK10 pKa = 10.19SSPSKK15 pKa = 9.67RR16 pKa = 11.84SPSTGHH22 pKa = 6.3VPFQGRR28 pKa = 11.84LDD30 pKa = 3.51MSLTIFTDD38 pKa = 3.62TPYY41 pKa = 11.48SSMEE45 pKa = 3.83EE46 pKa = 4.0AFLHH50 pKa = 6.56LIEE53 pKa = 4.43IQDD56 pKa = 4.29YY57 pKa = 10.47ISEE60 pKa = 4.18TRR62 pKa = 11.84DD63 pKa = 3.34LKK65 pKa = 11.16GLYY68 pKa = 9.7LGLIAVSILFSKK80 pKa = 10.75CDD82 pKa = 3.46ARR84 pKa = 11.84GSGVMYY90 pKa = 10.82TMDD93 pKa = 4.41LKK95 pKa = 11.33DD96 pKa = 4.11LVSLISTDD104 pKa = 3.13SALASGPLRR113 pKa = 11.84TFSYY117 pKa = 10.92EE118 pKa = 3.7RR119 pKa = 11.84THH121 pKa = 7.18DD122 pKa = 3.89LRR124 pKa = 11.84GKK126 pKa = 7.42TALVRR131 pKa = 11.84FRR133 pKa = 11.84LDD135 pKa = 3.61FEE137 pKa = 4.5QSGLDD142 pKa = 3.82CEE144 pKa = 4.79SLLSVLMKK152 pKa = 10.38RR153 pKa = 11.84NKK155 pKa = 9.87NKK157 pKa = 10.43EE158 pKa = 4.14VMSSSTSYY166 pKa = 11.12LKK168 pKa = 10.58LWRR171 pKa = 11.84LKK173 pKa = 10.39MIDD176 pKa = 3.84YY177 pKa = 10.71KK178 pKa = 10.92RR179 pKa = 11.84GTLFSLSS186 pKa = 3.5

Molecular weight: 20.98 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

3708

186

2136

741.6

83.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.529 ± 0.373

1.618 ± 0.243

5.583 ± 0.371

6.041 ± 0.795

4.477 ± 0.593

7.012 ± 0.073

2.05 ± 0.231

5.987 ± 0.587

5.717 ± 0.537

10.14 ± 0.401

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.238 ± 0.283

3.155 ± 0.358

5.097 ± 0.622

3.371 ± 0.232

5.906 ± 0.486

9.709 ± 0.83

5.609 ± 0.264

5.636 ± 0.416

1.888 ± 0.292

3.236 ± 0.177
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski