Elusimicrobium minutum (strain Pei191)

Taxonomy: cellular organisms; Bacteria; Elusimicrobia; Elusimicrobia; Elusimicrobiales; Elusimicrobiaceae; Elusimicrobium; Elusimicrobium minutum

Average proteome isoelectric point is 7.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1528 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B2KAP3|B2KAP3_ELUMP Glycoside hydrolase OS=Elusimicrobium minutum (strain Pei191) OX=445932 GN=Emin_0021 PE=4 SV=1
MM1 pKa = 7.15QKK3 pKa = 10.23YY4 pKa = 8.61VCNVCGYY11 pKa = 9.82IYY13 pKa = 10.52DD14 pKa = 4.32PAAGDD19 pKa = 3.82PDD21 pKa = 3.96SGITAGTPWEE31 pKa = 4.2QVPADD36 pKa = 4.27WVCPACGVGKK46 pKa = 9.99DD47 pKa = 3.72QFSPMM52 pKa = 4.05

Molecular weight: 5.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B2KEM7|B2KEM7_ELUMP Elongation factor G OS=Elusimicrobium minutum (strain Pei191) OX=445932 GN=fusA PE=3 SV=1
MM1 pKa = 7.12ATKK4 pKa = 10.36AWVAKK9 pKa = 8.15MAKK12 pKa = 7.89PQKK15 pKa = 10.1FAVRR19 pKa = 11.84YY20 pKa = 8.95HH21 pKa = 6.02NRR23 pKa = 11.84CQVCGRR29 pKa = 11.84PRR31 pKa = 11.84GYY33 pKa = 10.88YY34 pKa = 9.61RR35 pKa = 11.84DD36 pKa = 3.92FGLCRR41 pKa = 11.84ICLRR45 pKa = 11.84KK46 pKa = 8.66MAHH49 pKa = 5.98QGLIPGVRR57 pKa = 11.84KK58 pKa = 10.39SSWW61 pKa = 2.83

Molecular weight: 7.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1528

0

1528

493618

30

3965

323.0

35.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.378 ± 0.06

1.19 ± 0.039

4.881 ± 0.047

6.441 ± 0.06

4.95 ± 0.058

6.944 ± 0.056

1.502 ± 0.024

7.543 ± 0.053

8.841 ± 0.065

9.085 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.446 ± 0.024

4.985 ± 0.049

3.704 ± 0.039

2.883 ± 0.033

3.708 ± 0.041

6.148 ± 0.052

5.344 ± 0.047

6.636 ± 0.047

0.838 ± 0.02

3.551 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski