Fonsecaea pedrosoi CBS 271.37
Average proteome isoelectric point is 6.42
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 12525 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0D2GPT8|A0A0D2GPT8_9EURO Unplaced genomic scaffold supercont1.4 whole genome shotgun sequence OS=Fonsecaea pedrosoi CBS 271.37 OX=1442368 GN=Z517_07151 PE=3 SV=1
MM1 pKa = 7.2 YY2 pKa = 10.32 LLAGTAIAALATSCAASPGYY22 pKa = 10.46 LKK24 pKa = 10.94 LGLQRR29 pKa = 11.84 RR30 pKa = 11.84 APPEE34 pKa = 3.5 GRR36 pKa = 11.84 LIRR39 pKa = 11.84 RR40 pKa = 11.84 QDD42 pKa = 3.09 SGGSVDD48 pKa = 5.78 ALVTQNTNKK57 pKa = 10.35 AEE59 pKa = 4.04 YY60 pKa = 9.86 LVNITVGTPPQDD72 pKa = 3.48 LSVTLDD78 pKa = 3.4 TGSSDD83 pKa = 3.13 LWIPASSAKK92 pKa = 10.27 LCQQGKK98 pKa = 9.66 CDD100 pKa = 5.14 LGAFDD105 pKa = 4.95 PSKK108 pKa = 10.81 SSTYY112 pKa = 11.0 NVIEE116 pKa = 4.12 EE117 pKa = 4.38 GGFNITYY124 pKa = 9.51 VQVGDD129 pKa = 3.94 TDD131 pKa = 3.88 AGDD134 pKa = 3.49 WSSDD138 pKa = 3.4 TITVGGSPSIEE149 pKa = 3.93 DD150 pKa = 3.37 QQLGVATALFDD161 pKa = 3.29 QHH163 pKa = 7.62 GVMGIGFDD171 pKa = 3.94 TIEE174 pKa = 4.84 ASNDD178 pKa = 3.48 SPNGVYY184 pKa = 10.33 PSVLDD189 pKa = 3.72 NLKK192 pKa = 9.08 STGIIDD198 pKa = 3.89 RR199 pKa = 11.84 KK200 pKa = 10.69 AFSLYY205 pKa = 10.96 LNDD208 pKa = 5.03 LEE210 pKa = 4.33 ATKK213 pKa = 10.72 GAVIFGGIDD222 pKa = 3.15 TTKK225 pKa = 10.69 YY226 pKa = 10.78 SGDD229 pKa = 3.62 LVALPLQPGPDD240 pKa = 3.71 GSVSEE245 pKa = 4.44 YY246 pKa = 10.56 FVTLTSVSFTDD257 pKa = 3.34 STGQTTQLSPEE268 pKa = 4.79 GYY270 pKa = 10.08 AQAALLDD277 pKa = 4.09 SGTTNTLLTNDD288 pKa = 3.47 VLAPLALGLGAVVDD302 pKa = 4.57 PSGEE306 pKa = 4.13 AYY308 pKa = 10.42 LVPCSLSSANGTIDD322 pKa = 3.39 YY323 pKa = 11.06 SFGGEE328 pKa = 4.12 DD329 pKa = 4.51 GITIQVPVSQVVGGQVYY346 pKa = 8.74 TSSEE350 pKa = 3.9 FDD352 pKa = 3.99 DD353 pKa = 4.61 PSGGCVLAFGSTAEE367 pKa = 4.25 GAGASIFGDD376 pKa = 3.4 SFLRR380 pKa = 11.84 SAYY383 pKa = 10.44 AVFDD387 pKa = 4.17 IDD389 pKa = 3.55 NQMAALAQAVEE400 pKa = 4.61 NEE402 pKa = 4.61 DD403 pKa = 3.64 STSSIVVFPTGTTIPSATVTATATGTQLTEE433 pKa = 4.27 VVSEE437 pKa = 4.13 TDD439 pKa = 3.16 IPTASIQSSTLVPGTPTFSLEE460 pKa = 4.23 AASSTATTTGGSSSSSSSAAANNAVNAAIPTAALLGLGLAAAGMVGLL507 pKa = 4.75
Molecular weight: 51.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.697
IPC2_protein 3.694
IPC_protein 3.719
Toseland 3.49
ProMoST 3.884
Dawson 3.719
Bjellqvist 3.872
Wikipedia 3.656
Rodwell 3.541
Grimsley 3.401
Solomon 3.706
Lehninger 3.668
Nozaki 3.834
DTASelect 4.088
Thurlkill 3.541
EMBOSS 3.668
Sillero 3.846
Patrickios 0.947
IPC_peptide 3.706
IPC2_peptide 3.821
IPC2.peptide.svr19 3.759
Protein with the highest isoelectric point:
>tr|A0A0D2DUE2|A0A0D2DUE2_9EURO Unplaced genomic scaffold supercont1.3 whole genome shotgun sequence OS=Fonsecaea pedrosoi CBS 271.37 OX=1442368 GN=Z517_04416 PE=4 SV=1
MM1 pKa = 7.84 ASTTFLQRR9 pKa = 11.84 APKK12 pKa = 10.27 SSQQEE17 pKa = 3.81 LSSPNTHH24 pKa = 6.31 KK25 pKa = 10.79 RR26 pKa = 11.84 LASLPPEE33 pKa = 3.78 IHH35 pKa = 7.1 HH36 pKa = 7.12 IIADD40 pKa = 3.73 HH41 pKa = 6.86 LPYY44 pKa = 10.1 PDD46 pKa = 4.87 LLSLKK51 pKa = 9.81 LTDD54 pKa = 3.57 TYY56 pKa = 11.06 FAALVGPKK64 pKa = 9.06 LTVKK68 pKa = 10.54 SRR70 pKa = 11.84 VSWVQSRR77 pKa = 11.84 YY78 pKa = 8.63 AQRR81 pKa = 11.84 LPVPTSTKK89 pKa = 10.34 LSFRR93 pKa = 11.84 TDD95 pKa = 3.2 EE96 pKa = 4.53 LFVANPEE103 pKa = 3.86 VNAILRR109 pKa = 11.84 RR110 pKa = 11.84 RR111 pKa = 11.84 RR112 pKa = 11.84 RR113 pKa = 11.84 HH114 pKa = 5.9 LEE116 pKa = 3.69 CADD119 pKa = 3.47 RR120 pKa = 11.84 RR121 pKa = 11.84 EE122 pKa = 4.19 PKK124 pKa = 10.33 AALGFSAFTEE134 pKa = 3.72 VDD136 pKa = 3.34 RR137 pKa = 11.84 NVRR140 pKa = 11.84 RR141 pKa = 11.84 LQGAQQLDD149 pKa = 3.76 TTACLVTGAPNCPAIPNLDD168 pKa = 3.56 TKK170 pKa = 8.13 VQRR173 pKa = 11.84 YY174 pKa = 8.55 RR175 pKa = 11.84 NSVVSKK181 pKa = 8.81 LTAPVLRR188 pKa = 11.84 TVAWLRR194 pKa = 11.84 GLQRR198 pKa = 11.84 SFRR201 pKa = 11.84 VSLSCAVAEE210 pKa = 4.49 LSHH213 pKa = 6.03 TPRR216 pKa = 11.84 VLWSWFLATLKK227 pKa = 10.61 LAFIVVVASVACTVCGWRR245 pKa = 11.84 PPPVAFTNPP254 pKa = 3.13
Molecular weight: 28.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.353
IPC2_protein 9.56
IPC_protein 10.306
Toseland 10.672
ProMoST 10.35
Dawson 10.76
Bjellqvist 10.482
Wikipedia 10.965
Rodwell 10.95
Grimsley 10.804
Solomon 10.877
Lehninger 10.847
Nozaki 10.687
DTASelect 10.467
Thurlkill 10.672
EMBOSS 11.082
Sillero 10.701
Patrickios 10.672
IPC_peptide 10.891
IPC2_peptide 9.692
IPC2.peptide.svr19 8.612
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
12525
0
12525
6141607
50
6165
490.3
54.28
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.629 ± 0.019
1.239 ± 0.008
5.737 ± 0.016
6.052 ± 0.021
3.74 ± 0.013
6.899 ± 0.02
2.468 ± 0.009
4.866 ± 0.014
4.668 ± 0.02
8.984 ± 0.025
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.146 ± 0.007
3.541 ± 0.01
6.051 ± 0.024
4.093 ± 0.014
6.188 ± 0.021
8.097 ± 0.026
6.06 ± 0.016
6.279 ± 0.016
1.492 ± 0.008
2.77 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here