Streptomyces sp. CdTB01

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8721 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0U3GCQ3|A0A0U3GCQ3_9ACTN Queuosine biosynthesis protein OS=Streptomyces sp. CdTB01 OX=1725411 GN=AS200_34280 PE=4 SV=1
MM1 pKa = 7.43GSFYY5 pKa = 11.34GNVLVARR12 pKa = 11.84ACDD15 pKa = 3.61EE16 pKa = 4.3VVPLLDD22 pKa = 4.73GPDD25 pKa = 3.39GTGIRR30 pKa = 11.84GFALPVGPGHH40 pKa = 5.78TAVYY44 pKa = 9.48PDD46 pKa = 5.16PEE48 pKa = 4.23TDD50 pKa = 3.77APDD53 pKa = 3.47LAGPLSRR60 pKa = 11.84LLGAPALGSYY70 pKa = 10.54VFDD73 pKa = 3.8SDD75 pKa = 3.94VLIMHH80 pKa = 7.16LYY82 pKa = 9.38VDD84 pKa = 4.0GEE86 pKa = 4.12LRR88 pKa = 11.84HH89 pKa = 6.89AYY91 pKa = 9.94DD92 pKa = 2.84SWPGYY97 pKa = 9.93FDD99 pKa = 4.29EE100 pKa = 5.35PAADD104 pKa = 3.79GDD106 pKa = 4.52GDD108 pKa = 4.09PEE110 pKa = 4.31DD111 pKa = 4.32AGVFAFPEE119 pKa = 4.02PAGADD124 pKa = 3.33PEE126 pKa = 4.75AFVPLAFGPVDD137 pKa = 3.9RR138 pKa = 11.84EE139 pKa = 4.01ALEE142 pKa = 4.15SVLRR146 pKa = 11.84GTPLDD151 pKa = 4.56PGDD154 pKa = 4.3GQDD157 pKa = 3.52GRR159 pKa = 11.84YY160 pKa = 9.53VFANTQHH167 pKa = 6.71YY168 pKa = 8.5DD169 pKa = 3.09TMLCLGLNACRR180 pKa = 11.84LSTGYY185 pKa = 10.45HH186 pKa = 5.32YY187 pKa = 11.16LSLGGLPHH195 pKa = 5.62EE196 pKa = 4.78TKK198 pKa = 10.74AEE200 pKa = 3.91EE201 pKa = 4.04VLAIGG206 pKa = 4.77

Molecular weight: 21.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0U3Q0Z3|A0A0U3Q0Z3_9ACTN Uncharacterized protein OS=Streptomyces sp. CdTB01 OX=1725411 GN=AS200_15115 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILANRR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.46GRR40 pKa = 11.84ASLSAA45 pKa = 3.83

Molecular weight: 5.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8721

0

8721

2818862

29

4643

323.2

34.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.613 ± 0.038

0.8 ± 0.007

5.958 ± 0.022

5.535 ± 0.026

2.735 ± 0.015

9.333 ± 0.023

2.39 ± 0.013

3.086 ± 0.016

2.172 ± 0.021

10.439 ± 0.03

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.734 ± 0.012

1.776 ± 0.015

6.002 ± 0.023

2.799 ± 0.016

8.062 ± 0.03

5.081 ± 0.02

6.232 ± 0.024

8.544 ± 0.025

1.565 ± 0.011

2.144 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski