Rift valley fever virus (RVFV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina; Ellioviricetes; Bunyavirales; Phenuiviridae; Phlebovirus; Rift Valley fever phlebovirus

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F5BUA8|F5BUA8_RVFV Non-structural protein NS-S OS=Rift valley fever virus OX=11588 PE=3 SV=1
MM1 pKa = 8.36DD2 pKa = 4.9YY3 pKa = 10.96FPVISVDD10 pKa = 3.68LQSGRR15 pKa = 11.84RR16 pKa = 11.84VVSVEE21 pKa = 3.92YY22 pKa = 10.3IKK24 pKa = 11.2GDD26 pKa = 3.49GPPRR30 pKa = 11.84IPYY33 pKa = 10.8SMVGPCCVFLMHH45 pKa = 6.78HH46 pKa = 6.51RR47 pKa = 11.84PSHH50 pKa = 4.79EE51 pKa = 3.6VRR53 pKa = 11.84LRR55 pKa = 11.84FSDD58 pKa = 4.43FYY60 pKa = 11.69NVGEE64 pKa = 3.95FPYY67 pKa = 10.48RR68 pKa = 11.84VGLGDD73 pKa = 3.64FASNVAPPPAKK84 pKa = 10.09PFQRR88 pKa = 11.84LIDD91 pKa = 4.95LIGHH95 pKa = 5.42MTLSDD100 pKa = 3.52FTRR103 pKa = 11.84FPNLKK108 pKa = 9.9EE109 pKa = 4.39AISWPLGEE117 pKa = 4.82PSLAFFDD124 pKa = 4.54LSSTRR129 pKa = 11.84VHH131 pKa = 6.71RR132 pKa = 11.84NDD134 pKa = 5.09DD135 pKa = 3.07IRR137 pKa = 11.84RR138 pKa = 11.84DD139 pKa = 3.87QIATLAMRR147 pKa = 11.84SCKK150 pKa = 8.73ITNDD154 pKa = 3.67LEE156 pKa = 4.43DD157 pKa = 4.02SFVGLHH163 pKa = 5.96RR164 pKa = 11.84MIVTEE169 pKa = 4.83AILRR173 pKa = 11.84GIDD176 pKa = 3.45LCLLPGFDD184 pKa = 4.37LMYY187 pKa = 10.48EE188 pKa = 4.1IAHH191 pKa = 4.96VQCVRR196 pKa = 11.84LLQAARR202 pKa = 11.84EE203 pKa = 4.46DD204 pKa = 3.27ISNAVVPNSALIALMEE220 pKa = 4.46EE221 pKa = 4.44SLMLRR226 pKa = 11.84SSLPSMMGRR235 pKa = 11.84NNWIPVVPPIPDD247 pKa = 3.03VEE249 pKa = 4.3MEE251 pKa = 4.31SGEE254 pKa = 4.21EE255 pKa = 3.89SDD257 pKa = 5.34DD258 pKa = 4.08DD259 pKa = 4.64GFVEE263 pKa = 4.86VDD265 pKa = 2.97

Molecular weight: 29.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B1NHK9|B1NHK9_RVFV Glycoprotein OS=Rift valley fever virus OX=11588 PE=4 SV=1
MM1 pKa = 8.23DD2 pKa = 5.44NYY4 pKa = 10.84QEE6 pKa = 4.74LAIQFAAQAVDD17 pKa = 3.4RR18 pKa = 11.84NEE20 pKa = 3.46IEE22 pKa = 3.67QWVRR26 pKa = 11.84EE27 pKa = 4.01FAYY30 pKa = 10.43QGFDD34 pKa = 2.88ARR36 pKa = 11.84RR37 pKa = 11.84VIEE40 pKa = 4.31LLKK43 pKa = 10.74QYY45 pKa = 11.03GGADD49 pKa = 3.24WEE51 pKa = 4.25KK52 pKa = 10.47DD53 pKa = 3.28AKK55 pKa = 10.96KK56 pKa = 10.12MIVLALTRR64 pKa = 11.84GNKK67 pKa = 7.85PRR69 pKa = 11.84RR70 pKa = 11.84MMMKK74 pKa = 9.48MSKK77 pKa = 9.66EE78 pKa = 3.74GKK80 pKa = 8.44ATVEE84 pKa = 3.81ALINKK89 pKa = 8.82YY90 pKa = 9.39KK91 pKa = 10.67LKK93 pKa = 10.42EE94 pKa = 4.19GNPSRR99 pKa = 11.84DD100 pKa = 3.48EE101 pKa = 4.06LTLSRR106 pKa = 11.84VAAALAGWTCQALVVLSEE124 pKa = 4.06WLPVTGTTMDD134 pKa = 4.23GLSPAYY140 pKa = 8.43PRR142 pKa = 11.84HH143 pKa = 5.28MMHH146 pKa = 7.14PSFAGMVDD154 pKa = 3.6PSLPEE159 pKa = 4.75DD160 pKa = 3.82YY161 pKa = 11.02LRR163 pKa = 11.84AILDD167 pKa = 3.42AHH169 pKa = 6.41SLYY172 pKa = 10.89LLQFSRR178 pKa = 11.84VINPNLRR185 pKa = 11.84GRR187 pKa = 11.84TKK189 pKa = 10.89EE190 pKa = 3.78EE191 pKa = 3.56VAATFTQPMNAAVNSNFISHH211 pKa = 5.92EE212 pKa = 3.81KK213 pKa = 10.03RR214 pKa = 11.84RR215 pKa = 11.84EE216 pKa = 3.62FLKK219 pKa = 11.11AFGLVDD225 pKa = 4.28SNGKK229 pKa = 9.42PSAAVMAAAQAYY241 pKa = 6.46KK242 pKa = 9.66TAAA245 pKa = 3.42

Molecular weight: 27.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

3799

245

2092

949.8

106.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.554 ± 1.275

2.869 ± 0.992

5.528 ± 0.598

6.528 ± 0.406

4.712 ± 0.333

6.133 ± 0.669

2.422 ± 0.136

5.712 ± 0.512

6.107 ± 0.757

9.239 ± 0.249

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.264 ± 0.599

3.79 ± 0.177

4.37 ± 0.617

3.053 ± 0.233

5.291 ± 0.582

8.923 ± 0.669

4.738 ± 0.492

6.581 ± 0.546

1.29 ± 0.119

2.895 ± 0.234
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski