Nanhai ghost shark arterivirus

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Nidovirales; Nanidovirineae; Nanghoshaviridae; Chimanivirinae; Chimshavirus; Nangarvirus 1

Average proteome isoelectric point is 7.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2P1GMX9|A0A2P1GMX9_9NIDO Uncharacterized protein OS=Nanhai ghost shark arterivirus OX=2116441 PE=4 SV=1
MM1 pKa = 7.77CCFLLQLVVGCLIYY15 pKa = 10.86LSGVRR20 pKa = 11.84LGVGIPLLEE29 pKa = 4.38SQPLMLTPRR38 pKa = 11.84SFPDD42 pKa = 3.47SSWTAPIQRR51 pKa = 11.84PLTNYY56 pKa = 10.49SDD58 pKa = 4.14LEE60 pKa = 4.53DD61 pKa = 3.59YY62 pKa = 11.02FSFRR66 pKa = 11.84RR67 pKa = 11.84NCTDD71 pKa = 4.85LLDD74 pKa = 4.33HH75 pKa = 6.73MANSHH80 pKa = 5.58MCNEE84 pKa = 4.15TLACLSTLWLGPYY97 pKa = 8.14VTEE100 pKa = 4.5GTCEE104 pKa = 3.77INNLQGASVLAKK116 pKa = 9.97IFRR119 pKa = 11.84RR120 pKa = 11.84VQLAIRR126 pKa = 11.84NEE128 pKa = 4.19NQTPNCTNPGNLMPDD143 pKa = 2.99HH144 pKa = 6.14QTYY147 pKa = 10.19LWLVHH152 pKa = 6.3YY153 pKa = 8.01MPQHH157 pKa = 6.11HH158 pKa = 6.7RR159 pKa = 11.84RR160 pKa = 11.84ADD162 pKa = 3.42LASWLTHH169 pKa = 6.17CRR171 pKa = 11.84ILYY174 pKa = 8.33QRR176 pKa = 11.84EE177 pKa = 4.03YY178 pKa = 8.56THH180 pKa = 7.11PGGFQVQRR188 pKa = 11.84RR189 pKa = 11.84HH190 pKa = 5.7LKK192 pKa = 9.52IFGPFSSTRR201 pKa = 11.84VSGKK205 pKa = 9.32EE206 pKa = 3.65LSGLGLDD213 pKa = 4.72GDD215 pKa = 4.79LFDD218 pKa = 6.69DD219 pKa = 5.33PFDD222 pKa = 5.28GDD224 pKa = 4.94DD225 pKa = 4.09DD226 pKa = 5.76LFDD229 pKa = 5.54FEE231 pKa = 5.17MDD233 pKa = 4.32LDD235 pKa = 4.08PEE237 pKa = 4.26IWSSLVTEE245 pKa = 4.48PPGLEE250 pKa = 4.08LEE252 pKa = 4.67EE253 pKa = 5.36GIQPFPTNLSLTGRR267 pKa = 11.84DD268 pKa = 3.41DD269 pKa = 4.03LEE271 pKa = 3.86IRR273 pKa = 11.84KK274 pKa = 8.78FLDD277 pKa = 3.05NLQRR281 pKa = 11.84PQVYY285 pKa = 8.78TSAWTPKK292 pKa = 9.74HH293 pKa = 6.09PEE295 pKa = 3.53INEE298 pKa = 3.95QAFSAIRR305 pKa = 3.4

Molecular weight: 34.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2P1GND3|A0A2P1GND3_9NIDO Uncharacterized protein OS=Nanhai ghost shark arterivirus OX=2116441 PE=4 SV=1
MM1 pKa = 7.57TSFPSWTPEE10 pKa = 3.88ANSPNANNRR19 pKa = 11.84PFPPPPPVQVVYY31 pKa = 11.1VRR33 pKa = 11.84GGGGNWRR40 pKa = 11.84GGRR43 pKa = 11.84GRR45 pKa = 11.84SRR47 pKa = 11.84GRR49 pKa = 11.84GRR51 pKa = 11.84GYY53 pKa = 10.7GYY55 pKa = 10.45NQAPPQQQSPCRR67 pKa = 11.84TIPGFHH73 pKa = 6.62NFNGKK78 pKa = 8.69WYY80 pKa = 8.8PNDD83 pKa = 3.23SGYY86 pKa = 8.93TPKK89 pKa = 10.57QLGEE93 pKa = 4.38SLSNMHH99 pKa = 6.82KK100 pKa = 10.75ASTSEE105 pKa = 4.14HH106 pKa = 5.37NN107 pKa = 3.51

Molecular weight: 11.78 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

4251

107

3399

850.2

93.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.187 ± 0.857

2.117 ± 0.339

3.67 ± 0.617

4.846 ± 0.367

3.411 ± 0.728

7.551 ± 0.642

2.376 ± 0.325

4.54 ± 0.428

4.093 ± 0.639

12.021 ± 1.23

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.423 ± 0.174

2.893 ± 1.047

7.01 ± 0.64

3.693 ± 0.353

4.869 ± 0.601

7.222 ± 0.39

7.575 ± 0.46

8.516 ± 1.133

2.047 ± 0.542

2.94 ± 0.388
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski