Thermanaerovibrio acidaminovorans (strain ATCC 49978 / DSM 6589 / Su883) (Selenomonas acidaminovorans)
Average proteome isoelectric point is 6.6
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1737 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|D1B5I5|D1B5I5_THEAS Uncharacterized protein OS=Thermanaerovibrio acidaminovorans (strain ATCC 49978 / DSM 6589 / Su883) OX=525903 GN=Taci_1044 PE=4 SV=1
MM1 pKa = 7.84 SSRR4 pKa = 11.84 EE5 pKa = 3.81 KK6 pKa = 10.19 IAYY9 pKa = 9.53 LKK11 pKa = 11.08 GLIDD15 pKa = 3.9 GLKK18 pKa = 10.21 ISDD21 pKa = 3.9 PDD23 pKa = 3.44 MSKK26 pKa = 10.35 VLSAVVEE33 pKa = 4.34 SLDD36 pKa = 4.1 ALAEE40 pKa = 4.25 DD41 pKa = 5.46 LEE43 pKa = 4.43 NQALVVDD50 pKa = 4.55 EE51 pKa = 3.95 QRR53 pKa = 11.84 EE54 pKa = 4.09 VLEE57 pKa = 5.13 EE58 pKa = 3.64 ISSYY62 pKa = 11.18 LDD64 pKa = 3.27 QLDD67 pKa = 3.92 EE68 pKa = 5.72 DD69 pKa = 4.02 ISSLEE74 pKa = 4.02 DD75 pKa = 3.44 RR76 pKa = 11.84 VEE78 pKa = 4.28 PCCGHH83 pKa = 6.74 HH84 pKa = 6.01 GHH86 pKa = 7.28 HH87 pKa = 7.46 DD88 pKa = 3.7 GDD90 pKa = 4.34 DD91 pKa = 4.63 DD92 pKa = 4.6 EE93 pKa = 7.28 DD94 pKa = 4.69 EE95 pKa = 5.29 DD96 pKa = 4.57 DD97 pKa = 3.89 EE98 pKa = 5.56 EE99 pKa = 4.75 YY100 pKa = 11.6 VSVCCPHH107 pKa = 6.59 CGKK110 pKa = 10.41 DD111 pKa = 3.52 FFYY114 pKa = 11.17 DD115 pKa = 3.24 PDD117 pKa = 5.51 AYY119 pKa = 10.82 DD120 pKa = 3.52 EE121 pKa = 6.03 DD122 pKa = 4.85 EE123 pKa = 5.86 DD124 pKa = 5.25 LLCPHH129 pKa = 7.03 CGEE132 pKa = 4.27 PFDD135 pKa = 5.51 RR136 pKa = 11.84 SEE138 pKa = 3.78
Molecular weight: 15.56 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.719
IPC2_protein 3.808
IPC_protein 3.808
Toseland 3.605
ProMoST 3.948
Dawson 3.795
Bjellqvist 3.973
Wikipedia 3.719
Rodwell 3.643
Grimsley 3.516
Solomon 3.795
Lehninger 3.745
Nozaki 3.91
DTASelect 4.126
Thurlkill 3.643
EMBOSS 3.732
Sillero 3.935
Patrickios 0.896
IPC_peptide 3.795
IPC2_peptide 3.91
IPC2.peptide.svr19 3.844
Protein with the highest isoelectric point:
>tr|D1B9L7|D1B9L7_THEAS S-adenosylmethionine decarboxylase proenzyme OS=Thermanaerovibrio acidaminovorans (strain ATCC 49978 / DSM 6589 / Su883) OX=525903 GN=Taci_0737 PE=3 SV=1
MM1 pKa = 7.28 KK2 pKa = 9.44 RR3 pKa = 11.84 TYY5 pKa = 10.04 QPHH8 pKa = 4.86 NTPRR12 pKa = 11.84 KK13 pKa = 8.19 RR14 pKa = 11.84 SMGFLVRR21 pKa = 11.84 SASASGRR28 pKa = 11.84 RR29 pKa = 11.84 VLRR32 pKa = 11.84 NRR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 KK37 pKa = 8.69 GRR39 pKa = 11.84 KK40 pKa = 8.69 RR41 pKa = 11.84 LAVV44 pKa = 3.41
Molecular weight: 5.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.447
IPC2_protein 11.155
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.34
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.076
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.1
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1737
0
1737
566890
31
1651
326.4
35.8
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.862 ± 0.064
1.266 ± 0.021
5.386 ± 0.042
6.779 ± 0.055
3.545 ± 0.039
9.575 ± 0.065
1.814 ± 0.027
4.701 ± 0.041
3.579 ± 0.048
10.971 ± 0.061
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.187 ± 0.027
2.237 ± 0.032
4.957 ± 0.039
2.685 ± 0.03
7.947 ± 0.057
6.271 ± 0.05
3.87 ± 0.038
8.843 ± 0.056
1.265 ± 0.02
2.26 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here