Marinithermus hydrothermalis (strain DSM 14884 / JCM 11576 / T1)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Deinococcus-Thermus; Deinococci; Thermales; Thermaceae; Marinithermus; Marinithermus hydrothermalis

Average proteome isoelectric point is 6.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2194 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F2NQ84|F2NQ84_MARHT Transposase IS4 family protein OS=Marinithermus hydrothermalis (strain DSM 14884 / JCM 11576 / T1) OX=869210 GN=Marky_0645 PE=4 SV=1
MM1 pKa = 7.62RR2 pKa = 11.84RR3 pKa = 11.84ILLISVLGLLLAACNQNATSGSKK26 pKa = 9.65PAPSPKK32 pKa = 9.3NAKK35 pKa = 8.3VTVSVTFPEE44 pKa = 4.79SPSEE48 pKa = 3.97APYY51 pKa = 10.85SGLSSIWEE59 pKa = 4.25LFSNDD64 pKa = 3.93PKK66 pKa = 10.97DD67 pKa = 3.69IQPTGAPSSSQKK79 pKa = 11.11AVITVYY85 pKa = 10.53EE86 pKa = 4.45GSTPVTSATVDD97 pKa = 3.33RR98 pKa = 11.84YY99 pKa = 11.29NPTATVLLPAGKK111 pKa = 9.72SYY113 pKa = 10.89RR114 pKa = 11.84FEE116 pKa = 5.63ASVKK120 pKa = 10.18DD121 pKa = 3.88YY122 pKa = 11.58SDD124 pKa = 4.93VEE126 pKa = 4.65VAWGEE131 pKa = 3.86ATQAISGDD139 pKa = 3.64TSVTLSVQTILRR151 pKa = 11.84SALLIPVGGLTANNSEE167 pKa = 4.25VDD169 pKa = 3.62VFIYY173 pKa = 10.17PLTGITGASVPATDD187 pKa = 3.95YY188 pKa = 10.88EE189 pKa = 4.36VTYY192 pKa = 9.07TVQGGTILDD201 pKa = 3.55QNKK204 pKa = 9.84RR205 pKa = 11.84GVRR208 pKa = 11.84IAWDD212 pKa = 3.83GTSDD216 pKa = 3.78PVTVTAQVSGLGEE229 pKa = 4.03DD230 pKa = 4.16HH231 pKa = 7.05NPVTIEE237 pKa = 3.61RR238 pKa = 11.84VYY240 pKa = 11.06NITPATGQGAPLSITGLLPNWPSPFQAKK268 pKa = 7.46VTALDD273 pKa = 3.54VFGAPIYY280 pKa = 6.85EE281 pKa = 4.45TPVGTDD287 pKa = 3.18GSFSLQLPGGDD298 pKa = 3.84ALTPIAIDD306 pKa = 3.64LSEE309 pKa = 4.72LFAVLPQAGCTVFWTTEE326 pKa = 3.71PAPLLNATLVPGQFWIKK343 pKa = 10.77DD344 pKa = 3.56QFTDD348 pKa = 3.34SHH350 pKa = 7.32LGTALLTVSGYY361 pKa = 8.78TLDD364 pKa = 4.02AAFLYY369 pKa = 10.64VDD371 pKa = 4.49RR372 pKa = 11.84SVQAEE377 pKa = 4.16GNATCIAPDD386 pKa = 3.59DD387 pKa = 4.77RR388 pKa = 11.84IITVTVNLNLEE399 pKa = 4.49AGWNLVVSTGDD410 pKa = 3.24PYY412 pKa = 11.65SYY414 pKa = 10.11LTVTGTPLNGSFQAPYY430 pKa = 5.92TTPYY434 pKa = 10.36HH435 pKa = 6.27VIDD438 pKa = 4.29WTLQWHH444 pKa = 4.91TTITDD449 pKa = 3.85LSISAVPQSWAPSEE463 pKa = 4.43GGSFYY468 pKa = 10.82STFTVTNNGPTSLSSVQVALDD489 pKa = 3.69VPSGVTNFYY498 pKa = 11.28VDD500 pKa = 4.13VPNGWFDD507 pKa = 3.49SYY509 pKa = 12.02SNIVYY514 pKa = 10.12LYY516 pKa = 10.84DD517 pKa = 3.69SLAPGDD523 pKa = 3.76NTSFTVNGTTTSGTAGQTLTLSATIQTDD551 pKa = 3.62YY552 pKa = 10.96LTDD555 pKa = 3.55TNPNDD560 pKa = 3.12NTAIVTITPQGGSNANVSVGMDD582 pKa = 4.6LDD584 pKa = 4.52PPAVWFLSPSPGEE597 pKa = 4.33VIPQGSTYY605 pKa = 9.68TVQIEE610 pKa = 4.38VWDD613 pKa = 4.12SSSGEE618 pKa = 4.06SSTPDD623 pKa = 3.01VTEE626 pKa = 3.95IEE628 pKa = 4.92LYY630 pKa = 10.82DD631 pKa = 3.64GAQRR635 pKa = 11.84LGALSSGEE643 pKa = 3.84IVFNPTTNAYY653 pKa = 9.67EE654 pKa = 4.26FVWDD658 pKa = 4.38LTTASPKK665 pKa = 9.9RR666 pKa = 11.84HH667 pKa = 5.76YY668 pKa = 9.93LTVFAYY674 pKa = 7.95DD675 pKa = 3.27TAGNVGQAEE684 pKa = 4.26ISVQVQQ690 pKa = 3.02

Molecular weight: 73.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F2NMZ8|F2NMZ8_MARHT Peptidase M23 OS=Marinithermus hydrothermalis (strain DSM 14884 / JCM 11576 / T1) OX=869210 GN=Marky_2009 PE=4 SV=1
MM1 pKa = 7.61KK2 pKa = 8.93PTEE5 pKa = 4.94DD6 pKa = 3.87SPSSIVPCPHH16 pKa = 6.68CGATRR21 pKa = 11.84EE22 pKa = 4.07EE23 pKa = 4.22SGGYY27 pKa = 9.02RR28 pKa = 11.84RR29 pKa = 11.84RR30 pKa = 11.84KK31 pKa = 9.3LRR33 pKa = 11.84TFRR36 pKa = 11.84GIQEE40 pKa = 3.99VRR42 pKa = 11.84VKK44 pKa = 10.61RR45 pKa = 11.84IRR47 pKa = 11.84CAQCKK52 pKa = 7.23RR53 pKa = 11.84QKK55 pKa = 10.13RR56 pKa = 11.84ALYY59 pKa = 10.15PEE61 pKa = 4.55DD62 pKa = 3.9CPRR65 pKa = 11.84SRR67 pKa = 11.84WYY69 pKa = 10.42AISIQEE75 pKa = 4.24HH76 pKa = 5.34FLILASHH83 pKa = 7.28RR84 pKa = 11.84APEE87 pKa = 4.41SVQNDD92 pKa = 3.53LAKK95 pKa = 11.0NLGFPLTRR103 pKa = 11.84PTRR106 pKa = 11.84LRR108 pKa = 11.84WLRR111 pKa = 11.84SAGARR116 pKa = 11.84AKK118 pKa = 10.55RR119 pKa = 11.84LLQRR123 pKa = 11.84EE124 pKa = 3.91NRR126 pKa = 11.84LLRR129 pKa = 11.84GRR131 pKa = 11.84VYY133 pKa = 10.08WGSVDD138 pKa = 2.75EE139 pKa = 4.4WAFGRR144 pKa = 11.84GPKK147 pKa = 10.32GYY149 pKa = 10.35GYY151 pKa = 10.84LYY153 pKa = 10.21FEE155 pKa = 5.12AWTGCPLWGDD165 pKa = 3.73LGHH168 pKa = 6.52RR169 pKa = 11.84RR170 pKa = 11.84RR171 pKa = 11.84YY172 pKa = 9.06EE173 pKa = 3.84RR174 pKa = 11.84VRR176 pKa = 11.84EE177 pKa = 4.01LLWRR181 pKa = 11.84LPPRR185 pKa = 11.84LGVVSDD191 pKa = 4.46GAQEE195 pKa = 3.93VGEE198 pKa = 3.97ALGWLGRR205 pKa = 11.84RR206 pKa = 11.84LLWARR211 pKa = 11.84CAFHH215 pKa = 7.32LMRR218 pKa = 11.84EE219 pKa = 4.15VRR221 pKa = 11.84AKK223 pKa = 10.43VDD225 pKa = 3.74RR226 pKa = 11.84KK227 pKa = 10.6AVGGDD232 pKa = 3.2TGGSEE237 pKa = 4.33GAAGAWAWGEE247 pKa = 4.05GGVLLEE253 pKa = 4.63GASAPVGG260 pKa = 3.49

Molecular weight: 29.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2194

0

2194

688454

29

2681

313.8

34.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.313 ± 0.072

0.481 ± 0.014

4.004 ± 0.034

7.698 ± 0.064

3.51 ± 0.037

8.661 ± 0.058

2.083 ± 0.027

3.655 ± 0.043

2.744 ± 0.041

12.341 ± 0.071

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.656 ± 0.019

1.904 ± 0.027

6.143 ± 0.045

2.939 ± 0.03

8.498 ± 0.052

3.516 ± 0.028

4.817 ± 0.04

8.578 ± 0.05

1.569 ± 0.027

2.889 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski