Spodoptera frugiperda ascovirus 1a (SfAV-1a)
Average proteome isoelectric point is 7.14
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 123 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q0E514|Q0E514_SFAVA 28.8 kDa Fatty acid elongase OS=Spodoptera frugiperda ascovirus 1a OX=113370 GN=ORF087 PE=4 SV=1
MM1 pKa = 7.59 AMYY4 pKa = 10.33 DD5 pKa = 3.16 ITTSLDD11 pKa = 2.89 ACLYY15 pKa = 11.04 DD16 pKa = 3.74 MSDD19 pKa = 3.18 VSALCSRR26 pKa = 11.84 LNRR29 pKa = 11.84 LLSHH33 pKa = 6.91 RR34 pKa = 11.84 FGVVCEE40 pKa = 3.96 NGKK43 pKa = 10.27 VYY45 pKa = 11.09 SDD47 pKa = 3.53 IDD49 pKa = 3.68 TLAVLVRR56 pKa = 11.84 RR57 pKa = 11.84 SLVPRR62 pKa = 11.84 EE63 pKa = 4.28 VKK65 pKa = 10.54 DD66 pKa = 3.43 FAYY69 pKa = 10.19 GGRR72 pKa = 11.84 LEE74 pKa = 4.69 TYY76 pKa = 9.64 NEE78 pKa = 3.52 YY79 pKa = 10.8 DD80 pKa = 3.66 EE81 pKa = 5.8 RR82 pKa = 11.84 EE83 pKa = 4.03 KK84 pKa = 11.23 SCIRR88 pKa = 11.84 NLFSRR93 pKa = 11.84 CIGDD97 pKa = 4.06 DD98 pKa = 3.63 GSDD101 pKa = 3.46 VEE103 pKa = 4.78 LQRR106 pKa = 11.84 NMNVDD111 pKa = 3.54 SLDD114 pKa = 3.86 TIIVPGLYY122 pKa = 10.15 CAFFINSWYY131 pKa = 10.69 RR132 pKa = 11.84 FDD134 pKa = 5.33 NVFDD138 pKa = 4.39 RR139 pKa = 11.84 NAIEE143 pKa = 4.32 VNLTAARR150 pKa = 11.84 TNMVNPVLITHH161 pKa = 6.48 CVVCAEE167 pKa = 4.58 SEE169 pKa = 4.17 LDD171 pKa = 4.68 PLLLWVYY178 pKa = 11.03 SRR180 pKa = 11.84 ACEE183 pKa = 3.84 FRR185 pKa = 11.84 RR186 pKa = 11.84 NLFMMRR192 pKa = 11.84 RR193 pKa = 11.84 EE194 pKa = 4.11 LVGEE198 pKa = 4.1 SDD200 pKa = 4.24 DD201 pKa = 4.33 ADD203 pKa = 3.74 SAFSDD208 pKa = 3.88 SFEE211 pKa = 4.48 TDD213 pKa = 3.64 DD214 pKa = 4.9 EE215 pKa = 6.28 DD216 pKa = 5.49 DD217 pKa = 3.61 VV218 pKa = 4.21
Molecular weight: 25.0 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.278
IPC2_protein 4.329
IPC_protein 4.291
Toseland 4.101
ProMoST 4.431
Dawson 4.266
Bjellqvist 4.418
Wikipedia 4.177
Rodwell 4.126
Grimsley 4.012
Solomon 4.266
Lehninger 4.228
Nozaki 4.38
DTASelect 4.609
Thurlkill 4.126
EMBOSS 4.19
Sillero 4.406
Patrickios 3.35
IPC_peptide 4.266
IPC2_peptide 4.393
IPC2.peptide.svr19 4.348
Protein with the highest isoelectric point:
>tr|Q0E4Z0|Q0E4Z0_SFAVA 9.3 kDa OS=Spodoptera frugiperda ascovirus 1a OX=113370 GN=ORF111 PE=4 SV=1
MM1 pKa = 7.4 NVTKK5 pKa = 10.89 LLRR8 pKa = 11.84 SYY10 pKa = 10.93 NAAFTVATSTLLAPFTVMARR30 pKa = 11.84 GMNVFGAEE38 pKa = 4.04 SAIQFWLNVLLCCLRR53 pKa = 11.84 SYY55 pKa = 11.73 GMTAGIRR62 pKa = 11.84 LLYY65 pKa = 10.53 DD66 pKa = 3.44 PAVLTSKK73 pKa = 10.69 RR74 pKa = 11.84 IVVVSNHH81 pKa = 4.48 VNYY84 pKa = 10.66 YY85 pKa = 10.33 DD86 pKa = 3.82 WLVLWSCMLKK96 pKa = 10.47 LKK98 pKa = 10.37 RR99 pKa = 11.84 KK100 pKa = 9.71 NVVFCAKK107 pKa = 9.67 RR108 pKa = 11.84 HH109 pKa = 5.12 RR110 pKa = 11.84 NKK112 pKa = 10.82 GGFGHH117 pKa = 6.83 MLNTCMKK124 pKa = 9.02 ASGFIVLVTEE134 pKa = 4.06 HH135 pKa = 6.07 QRR137 pKa = 11.84 RR138 pKa = 11.84 QRR140 pKa = 11.84 DD141 pKa = 3.38 SGPRR145 pKa = 11.84 GGEE148 pKa = 3.26 IATIGRR154 pKa = 11.84 VLRR157 pKa = 11.84 LSISRR162 pKa = 11.84 SRR164 pKa = 11.84 LLNSATTSAVQLIEE178 pKa = 4.22 SKK180 pKa = 10.53 KK181 pKa = 9.85 AARR184 pKa = 11.84 STDD187 pKa = 3.06 SVLRR191 pKa = 11.84 ANTSSQTTPKK201 pKa = 9.1 VTDD204 pKa = 3.58 VPVSSTPTLLPPRR217 pKa = 11.84 TRR219 pKa = 11.84 GFQILMSALGEE230 pKa = 4.26 SCDD233 pKa = 3.61 AVVDD237 pKa = 3.93 VTLSYY242 pKa = 11.06 SYY244 pKa = 10.97 DD245 pKa = 3.63 VNVTLPKK252 pKa = 9.48 SHH254 pKa = 5.84 QVPRR258 pKa = 11.84 SVRR261 pKa = 11.84 VYY263 pKa = 9.15 MRR265 pKa = 11.84 KK266 pKa = 10.09 LNVTFNTSEE275 pKa = 3.92 TDD277 pKa = 3.96 GYY279 pKa = 11.45 ARR281 pKa = 11.84 WLRR284 pKa = 11.84 EE285 pKa = 3.51 WFVQKK290 pKa = 10.87 SLFIDD295 pKa = 3.88 DD296 pKa = 4.52 LRR298 pKa = 11.84 SGFDD302 pKa = 3.17 VIVPEE307 pKa = 4.01 NRR309 pKa = 11.84 RR310 pKa = 11.84 TLTIEE315 pKa = 3.9 PSPLLRR321 pKa = 11.84 RR322 pKa = 11.84 MYY324 pKa = 8.39 TTVPSACLATTLLAMFTLVLKK345 pKa = 10.64 GKK347 pKa = 10.48 SITFRR352 pKa = 11.84 RR353 pKa = 11.84 KK354 pKa = 9.25 AA355 pKa = 3.17
Molecular weight: 39.77 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.343
IPC2_protein 9.706
IPC_protein 10.409
Toseland 10.584
ProMoST 10.262
Dawson 10.701
Bjellqvist 10.409
Wikipedia 10.906
Rodwell 10.965
Grimsley 10.76
Solomon 10.789
Lehninger 10.76
Nozaki 10.584
DTASelect 10.394
Thurlkill 10.599
EMBOSS 10.979
Sillero 10.628
Patrickios 10.657
IPC_peptide 10.789
IPC2_peptide 9.487
IPC2.peptide.svr19 8.541
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
123
0
123
37551
65
1157
305.3
34.59
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.255 ± 0.169
2.812 ± 0.128
6.618 ± 0.147
4.903 ± 0.178
3.417 ± 0.127
4.961 ± 0.17
2.498 ± 0.129
4.876 ± 0.125
4.495 ± 0.15
8.263 ± 0.189
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.807 ± 0.095
5.057 ± 0.14
3.784 ± 0.152
2.546 ± 0.112
8.271 ± 0.29
8.122 ± 0.304
6.873 ± 0.149
8.503 ± 0.172
0.956 ± 0.064
3.981 ± 0.157
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here