Roseburia intestinalis CAG:13

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Roseburia; environmental samples

Average proteome isoelectric point is 5.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2856 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R6AYL2|R6AYL2_9FIRM Uncharacterized protein OS=Roseburia intestinalis CAG:13 OX=1263104 GN=BN484_02606 PE=4 SV=1
MM1 pKa = 7.24KK2 pKa = 10.32KK3 pKa = 10.18KK4 pKa = 10.69VVVLMLCTMMAVTALGCGGKK24 pKa = 10.39KK25 pKa = 10.15NDD27 pKa = 3.74TAEE30 pKa = 4.12GTEE33 pKa = 3.94AAEE36 pKa = 4.36SVNGTEE42 pKa = 4.17TAEE45 pKa = 4.05GTEE48 pKa = 4.24TVEE51 pKa = 4.31YY52 pKa = 10.11EE53 pKa = 4.32SPSYY57 pKa = 11.19DD58 pKa = 4.56LDD60 pKa = 4.16PSDD63 pKa = 4.86YY64 pKa = 10.44VTLCDD69 pKa = 3.73YY70 pKa = 11.43SKK72 pKa = 11.38VPVTITGDD80 pKa = 3.66YY81 pKa = 11.21DD82 pKa = 5.15VDD84 pKa = 3.78DD85 pKa = 4.82QDD87 pKa = 3.85AKK89 pKa = 11.42DD90 pKa = 3.69YY91 pKa = 10.5FEE93 pKa = 5.98QMFSYY98 pKa = 10.73YY99 pKa = 10.96GPFYY103 pKa = 11.03VADD106 pKa = 3.83DD107 pKa = 3.9TKK109 pKa = 9.42TTVGDD114 pKa = 3.66GDD116 pKa = 4.22IVNVDD121 pKa = 3.56YY122 pKa = 11.22VGKK125 pKa = 10.27LDD127 pKa = 5.47GVAFDD132 pKa = 4.43NGSAEE137 pKa = 4.13DD138 pKa = 3.7QNIDD142 pKa = 3.39VDD144 pKa = 4.49NNCSAGSQSSSFIDD158 pKa = 4.73GFTDD162 pKa = 3.53DD163 pKa = 5.28LKK165 pKa = 11.05GASVGDD171 pKa = 4.12VIDD174 pKa = 4.36SDD176 pKa = 4.13VTFPDD181 pKa = 3.2NYY183 pKa = 11.1GNADD187 pKa = 3.7LAGKK191 pKa = 9.3QVVFTFTVNSIQKK204 pKa = 10.05EE205 pKa = 4.15MTLDD209 pKa = 4.28DD210 pKa = 4.13VDD212 pKa = 5.87DD213 pKa = 5.83DD214 pKa = 4.22FAQKK218 pKa = 10.09QFQADD223 pKa = 3.93TVDD226 pKa = 5.17DD227 pKa = 4.25MYY229 pKa = 11.9AQIKK233 pKa = 10.37SFLEE237 pKa = 4.19SQASSNKK244 pKa = 10.38DD245 pKa = 2.81SDD247 pKa = 3.72TYY249 pKa = 10.96TAVQEE254 pKa = 4.18YY255 pKa = 10.58LLEE258 pKa = 4.26NCKK261 pKa = 10.17VDD263 pKa = 3.37IPEE266 pKa = 5.19DD267 pKa = 3.69YY268 pKa = 10.21LTARR272 pKa = 11.84VNDD275 pKa = 3.59YY276 pKa = 10.49EE277 pKa = 4.14KK278 pKa = 10.95QYY280 pKa = 9.39VQNYY284 pKa = 9.16CDD286 pKa = 3.88GDD288 pKa = 3.61ASKK291 pKa = 11.44LEE293 pKa = 4.98DD294 pKa = 3.81YY295 pKa = 11.18VSSQYY300 pKa = 11.91NMTLDD305 pKa = 3.79EE306 pKa = 5.39VRR308 pKa = 11.84QEE310 pKa = 4.01WKK312 pKa = 10.68SGMEE316 pKa = 4.17KK317 pKa = 10.2NISMEE322 pKa = 4.63FITGAIAAKK331 pKa = 10.17EE332 pKa = 4.06GTEE335 pKa = 3.79LDD337 pKa = 3.41EE338 pKa = 6.79DD339 pKa = 4.33GFSSYY344 pKa = 10.62VQTLMSNNSLSSEE357 pKa = 3.81DD358 pKa = 4.87DD359 pKa = 3.43LYY361 pKa = 11.86KK362 pKa = 11.02NYY364 pKa = 10.75GYY366 pKa = 11.26GDD368 pKa = 3.36AAYY371 pKa = 10.06GEE373 pKa = 4.59KK374 pKa = 10.55YY375 pKa = 10.12LRR377 pKa = 11.84QVYY380 pKa = 9.92VDD382 pKa = 4.1NLALQSVKK390 pKa = 11.01DD391 pKa = 3.89NADD394 pKa = 3.4VTVEE398 pKa = 4.23APAEE402 pKa = 4.25TEE404 pKa = 4.08STEE407 pKa = 4.24GTEE410 pKa = 4.11SAEE413 pKa = 3.92PQEE416 pKa = 4.81AVDD419 pKa = 3.58AAEE422 pKa = 4.37TEE424 pKa = 4.46TANN427 pKa = 4.27

Molecular weight: 46.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R6B584|R6B584_9FIRM ABC transporter permease/ATP-binding protein OS=Roseburia intestinalis CAG:13 OX=1263104 GN=BN484_02646 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 8.95KK9 pKa = 7.58RR10 pKa = 11.84QRR12 pKa = 11.84SKK14 pKa = 9.0VHH16 pKa = 5.93GFRR19 pKa = 11.84SRR21 pKa = 11.84MSTAGGRR28 pKa = 11.84KK29 pKa = 8.62VLAARR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.61GRR39 pKa = 11.84KK40 pKa = 8.87KK41 pKa = 10.59LSAA44 pKa = 3.95

Molecular weight: 5.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2856

0

2856

948991

29

2374

332.3

37.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.63 ± 0.049

1.476 ± 0.02

5.81 ± 0.04

7.78 ± 0.053

4.08 ± 0.035

7.042 ± 0.038

1.756 ± 0.019

7.257 ± 0.039

6.959 ± 0.041

8.635 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.231 ± 0.024

4.419 ± 0.031

3.053 ± 0.022

3.272 ± 0.029

4.067 ± 0.034

5.657 ± 0.037

5.612 ± 0.042

7.034 ± 0.038

0.916 ± 0.016

4.308 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski