Amino acid dipepetide frequency for Roseburia intestinalis CAG:13

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	7.818AlaAla: 7.818 ± 0.142	1.062AlaCys: 1.062 ± 0.04	5.061AlaAsp: 5.061 ± 0.084	5.866AlaGlu: 5.866 ± 0.094	3.079AlaPhe: 3.079 ± 0.063	6.598AlaGly: 6.598 ± 0.092	1.094AlaHis: 1.094 ± 0.037	4.779AlaIle: 4.779 ± 0.079	5.301AlaLys: 5.301 ± 0.091	6.476AlaLeu: 6.476 ± 0.092	2.504AlaMet: 2.504 ± 0.051	2.583AlaAsn: 2.583 ± 0.055	1.756AlaPro: 1.756 ± 0.048	2.376AlaGln: 2.376 ± 0.05	2.562AlaArg: 2.562 ± 0.052	3.981AlaSer: 3.981 ± 0.07	3.285AlaThr: 3.285 ± 0.076	6.594AlaVal: 6.594 ± 0.102	0.628AlaTrp: 0.628 ± 0.023	2.893AlaTyr: 2.893 ± 0.053	0.002AlaXaa: 0.002 ± 0.001
Cys	1.03CysAla: 1.03 ± 0.033	0.272CysCys: 0.272 ± 0.018	0.848CysAsp: 0.848 ± 0.032	0.993CysGlu: 0.993 ± 0.033	0.616CysPhe: 0.616 ± 0.024	1.509CysGly: 1.509 ± 0.04	0.299CysHis: 0.299 ± 0.016	1.146CysIle: 1.146 ± 0.035	0.836CysLys: 0.836 ± 0.031	1.141CysLeu: 1.141 ± 0.039	0.53CysMet: 0.53 ± 0.026	0.579CysAsn: 0.579 ± 0.023	0.571CysPro: 0.571 ± 0.026	0.364CysGln: 0.364 ± 0.019	0.684CysArg: 0.684 ± 0.029	0.849CysSer: 0.849 ± 0.031	0.671CysThr: 0.671 ± 0.03	1.059CysVal: 1.059 ± 0.035	0.12CysTrp: 0.12 ± 0.011	0.646CysTyr: 0.646 ± 0.026	0.0CysXaa: 0.0 ± 0.0
Asp	4.833AspAla: 4.833 ± 0.089	0.819AspCys: 0.819 ± 0.03	3.102AspAsp: 3.102 ± 0.074	5.015AspGlu: 5.015 ± 0.088	2.663AspPhe: 2.663 ± 0.057	4.689AspGly: 4.689 ± 0.09	0.975AspHis: 0.975 ± 0.03	4.554AspIle: 4.554 ± 0.071	3.324AspLys: 3.324 ± 0.066	4.487AspLeu: 4.487 ± 0.076	1.95AspMet: 1.95 ± 0.043	2.27AspAsn: 2.27 ± 0.058	1.671AspPro: 1.671 ± 0.044	1.459AspGln: 1.459 ± 0.038	2.201AspArg: 2.201 ± 0.049	3.058AspSer: 3.058 ± 0.062	3.497AspThr: 3.497 ± 0.063	4.045AspVal: 4.045 ± 0.071	0.594AspTrp: 0.594 ± 0.029	2.891AspTyr: 2.891 ± 0.051	0.0AspXaa: 0.0 ± 0.0
Glu	5.333GluAla: 5.333 ± 0.084	0.883GluCys: 0.883 ± 0.033	4.414GluAsp: 4.414 ± 0.082	7.181GluGlu: 7.181 ± 0.117	2.511GluPhe: 2.511 ± 0.054	4.217GluGly: 4.217 ± 0.067	1.586GluHis: 1.586 ± 0.045	5.608GluIle: 5.608 ± 0.088	7.398GluLys: 7.398 ± 0.107	6.803GluLeu: 6.803 ± 0.103	2.636GluMet: 2.636 ± 0.053	4.549GluAsn: 4.549 ± 0.071	1.849GluPro: 1.849 ± 0.046	3.356GluGln: 3.356 ± 0.065	3.378GluArg: 3.378 ± 0.072	3.552GluSer: 3.552 ± 0.068	4.181GluThr: 4.181 ± 0.082	4.564GluVal: 4.564 ± 0.082	0.675GluTrp: 0.675 ± 0.031	3.122GluTyr: 3.122 ± 0.065	0.001GluXaa: 0.001 ± 0.001
Phe	3.076PheAla: 3.076 ± 0.057	0.802PheCys: 0.802 ± 0.032	2.544PheAsp: 2.544 ± 0.057	2.739PheGlu: 2.739 ± 0.055	1.983PhePhe: 1.983 ± 0.054	3.007PheGly: 3.007 ± 0.063	0.886PheHis: 0.886 ± 0.031	2.727PheIle: 2.727 ± 0.059	1.918PheLys: 1.918 ± 0.045	3.987PheLeu: 3.987 ± 0.082	1.202PheMet: 1.202 ± 0.038	1.376PheAsn: 1.376 ± 0.037	1.317PhePro: 1.317 ± 0.036	1.184PheGln: 1.184 ± 0.031	1.573PheArg: 1.573 ± 0.042	2.863PheSer: 2.863 ± 0.068	2.311PheThr: 2.311 ± 0.049	2.971PheVal: 2.971 ± 0.057	0.462PheTrp: 0.462 ± 0.024	1.869PheTyr: 1.869 ± 0.053	0.0PheXaa: 0.0 ± 0.0
Gly	5.04GlyAla: 5.04 ± 0.084	1.286GlyCys: 1.286 ± 0.042	3.562GlyAsp: 3.562 ± 0.066	4.837GlyGlu: 4.837 ± 0.081	3.039GlyPhe: 3.039 ± 0.052	4.658GlyGly: 4.658 ± 0.09	1.222GlyHis: 1.222 ± 0.034	6.129GlyIle: 6.129 ± 0.081	5.618GlyLys: 5.618 ± 0.093	5.38GlyLeu: 5.38 ± 0.087	2.678GlyMet: 2.678 ± 0.064	3.281GlyAsn: 3.281 ± 0.065	1.126GlyPro: 1.126 ± 0.044	2.038GlyGln: 2.038 ± 0.05	2.845GlyArg: 2.845 ± 0.059	3.957GlySer: 3.957 ± 0.063	4.493GlyThr: 4.493 ± 0.088	5.138GlyVal: 5.138 ± 0.084	0.7GlyTrp: 0.7 ± 0.028	3.392GlyTyr: 3.392 ± 0.052	0.005GlyXaa: 0.005 ± 0.002
His	1.3HisAla: 1.3 ± 0.039	0.272HisCys: 0.272 ± 0.017	0.966HisAsp: 0.966 ± 0.04	1.109HisGlu: 1.109 ± 0.035	0.962HisPhe: 0.962 ± 0.027	1.239HisGly: 1.239 ± 0.041	0.416HisHis: 0.416 ± 0.025	1.373HisIle: 1.373 ± 0.04	0.968HisLys: 0.968 ± 0.036	1.548HisLeu: 1.548 ± 0.045	0.551HisMet: 0.551 ± 0.024	0.705HisAsn: 0.705 ± 0.026	0.807HisPro: 0.807 ± 0.032	0.563HisGln: 0.563 ± 0.025	0.765HisArg: 0.765 ± 0.028	0.888HisSer: 0.888 ± 0.033	1.003HisThr: 1.003 ± 0.03	1.15HisVal: 1.15 ± 0.037	0.18HisTrp: 0.18 ± 0.013	0.797HisTyr: 0.797 ± 0.03	0.0HisXaa: 0.0 ± 0.0
Ile	5.824IleAla: 5.824 ± 0.096	1.364IleCys: 1.364 ± 0.037	3.953IleAsp: 3.953 ± 0.074	4.719IleGlu: 4.719 ± 0.081	2.964IlePhe: 2.964 ± 0.073	5.219IleGly: 5.219 ± 0.09	1.332IleHis: 1.332 ± 0.038	4.8IleIle: 4.8 ± 0.091	4.005IleLys: 4.005 ± 0.07	7.014IleLeu: 7.014 ± 0.103	1.98IleMet: 1.98 ± 0.046	2.858IleAsn: 2.858 ± 0.062	3.051IlePro: 3.051 ± 0.056	2.1IleGln: 2.1 ± 0.05	3.641IleArg: 3.641 ± 0.062	4.864IleSer: 4.864 ± 0.075	4.387IleThr: 4.387 ± 0.086	4.953IleVal: 4.953 ± 0.081	0.659IleTrp: 0.659 ± 0.024	2.883IleTyr: 2.883 ± 0.059	0.0IleXaa: 0.0 ± 0.0
Lys	4.726LysAla: 4.726 ± 0.07	0.683LysCys: 0.683 ± 0.03	4.03LysAsp: 4.03 ± 0.063	6.903LysGlu: 6.903 ± 0.096	1.829LysPhe: 1.829 ± 0.047	3.83LysGly: 3.83 ± 0.068	1.059LysHis: 1.059 ± 0.033	5.285LysIle: 5.285 ± 0.075	6.795LysLys: 6.795 ± 0.109	5.316LysLeu: 5.316 ± 0.075	2.365LysMet: 2.365 ± 0.052	4.153LysAsn: 4.153 ± 0.077	1.974LysPro: 1.974 ± 0.045	2.522LysGln: 2.522 ± 0.059	2.989LysArg: 2.989 ± 0.062	3.206LysSer: 3.206 ± 0.058	3.935LysThr: 3.935 ± 0.079	4.439LysVal: 4.439 ± 0.094	0.586LysTrp: 0.586 ± 0.028	2.963LysTyr: 2.963 ± 0.066	0.003LysXaa: 0.003 ± 0.002
Leu	6.735LeuAla: 6.735 ± 0.09	1.483LeuCys: 1.483 ± 0.042	4.929LeuAsp: 4.929 ± 0.083	5.987LeuGlu: 5.987 ± 0.079	3.803LeuPhe: 3.803 ± 0.086	5.54LeuGly: 5.54 ± 0.089	1.487LeuHis: 1.487 ± 0.041	5.764LeuIle: 5.764 ± 0.095	6.204LeuLys: 6.204 ± 0.086	8.251LeuLeu: 8.251 ± 0.13	2.616LeuMet: 2.616 ± 0.062	3.772LeuAsn: 3.772 ± 0.062	3.312LeuPro: 3.312 ± 0.065	2.603LeuGln: 2.603 ± 0.058	3.322LeuArg: 3.322 ± 0.058	5.926LeuSer: 5.926 ± 0.081	5.074LeuThr: 5.074 ± 0.082	5.371LeuVal: 5.371 ± 0.082	0.74LeuTrp: 0.74 ± 0.03	3.435LeuTyr: 3.435 ± 0.067	0.0LeuXaa: 0.0 ± 0.0
Met	2.459MetAla: 2.459 ± 0.05	0.333MetCys: 0.333 ± 0.017	2.007MetAsp: 2.007 ± 0.047	2.753MetGlu: 2.753 ± 0.057	1.122MetPhe: 1.122 ± 0.036	2.136MetGly: 2.136 ± 0.054	0.562MetHis: 0.562 ± 0.03	2.292MetIle: 2.292 ± 0.052	2.506MetLys: 2.506 ± 0.046	2.858MetLeu: 2.858 ± 0.066	1.023MetMet: 1.023 ± 0.034	1.585MetAsn: 1.585 ± 0.044	1.23MetPro: 1.23 ± 0.037	1.356MetGln: 1.356 ± 0.037	1.277MetArg: 1.277 ± 0.039	1.841MetSer: 1.841 ± 0.043	1.848MetThr: 1.848 ± 0.045	1.95MetVal: 1.95 ± 0.049	0.192MetTrp: 0.192 ± 0.015	0.983MetTyr: 0.983 ± 0.032	0.0MetXaa: 0.0 ± 0.0
Asn	3.332AsnAla: 3.332 ± 0.062	0.617AsnCys: 0.617 ± 0.026	2.258AsnAsp: 2.258 ± 0.054	3.115AsnGlu: 3.115 ± 0.061	1.649AsnPhe: 1.649 ± 0.042	3.682AsnGly: 3.682 ± 0.068	0.813AsnHis: 0.813 ± 0.031	3.402AsnIle: 3.402 ± 0.066	2.483AsnLys: 2.483 ± 0.051	3.615AsnLeu: 3.615 ± 0.059	1.426AsnMet: 1.426 ± 0.042	1.848AsnAsn: 1.848 ± 0.055	1.792AsnPro: 1.792 ± 0.049	1.547AsnGln: 1.547 ± 0.045	1.983AsnArg: 1.983 ± 0.047	2.372AsnSer: 2.372 ± 0.058	2.641AsnThr: 2.641 ± 0.077	3.119AsnVal: 3.119 ± 0.059	0.444AsnTrp: 0.444 ± 0.023	2.054AsnTyr: 2.054 ± 0.05	0.0AsnXaa: 0.0 ± 0.0
Pro	2.283ProAla: 2.283 ± 0.055	0.422ProCys: 0.422 ± 0.022	2.304ProAsp: 2.304 ± 0.046	3.144ProGlu: 3.144 ± 0.065	1.388ProPhe: 1.388 ± 0.039	2.168ProGly: 2.168 ± 0.051	0.522ProHis: 0.522 ± 0.022	1.817ProIle: 1.817 ± 0.05	1.871ProLys: 1.871 ± 0.047	2.452ProLeu: 2.452 ± 0.058	0.89ProMet: 0.89 ± 0.037	1.092ProAsn: 1.092 ± 0.034	0.599ProPro: 0.599 ± 0.028	1.007ProGln: 1.007 ± 0.036	0.811ProArg: 0.811 ± 0.026	1.566ProSer: 1.566 ± 0.043	1.409ProThr: 1.409 ± 0.041	3.006ProVal: 3.006 ± 0.059	0.331ProTrp: 0.331 ± 0.019	1.446ProTyr: 1.446 ± 0.039	0.001ProXaa: 0.001 ± 0.001
Gln	2.472GlnAla: 2.472 ± 0.053	0.356GlnCys: 0.356 ± 0.02	1.528GlnAsp: 1.528 ± 0.044	2.67GlnGlu: 2.67 ± 0.056	1.231GlnPhe: 1.231 ± 0.034	1.864GlnGly: 1.864 ± 0.046	0.454GlnHis: 0.454 ± 0.024	2.748GlnIle: 2.748 ± 0.063	2.962GlnLys: 2.962 ± 0.064	2.655GlnLeu: 2.655 ± 0.061	1.319GlnMet: 1.319 ± 0.042	1.781GlnAsn: 1.781 ± 0.049	0.879GlnPro: 0.879 ± 0.034	1.252GlnGln: 1.252 ± 0.04	1.213GlnArg: 1.213 ± 0.034	1.605GlnSer: 1.605 ± 0.042	2.005GlnThr: 2.005 ± 0.052	2.074GlnVal: 2.074 ± 0.051	0.272GlnTrp: 0.272 ± 0.019	1.377GlnTyr: 1.377 ± 0.038	0.001GlnXaa: 0.001 ± 0.001
Arg	2.57ArgAla: 2.57 ± 0.048	0.515ArgCys: 0.515 ± 0.024	2.134ArgAsp: 2.134 ± 0.053	3.685ArgGlu: 3.685 ± 0.081	1.713ArgPhe: 1.713 ± 0.041	2.337ArgGly: 2.337 ± 0.058	0.785ArgHis: 0.785 ± 0.029	3.334ArgIle: 3.334 ± 0.059	3.204ArgLys: 3.204 ± 0.06	3.511ArgLeu: 3.511 ± 0.074	1.535ArgMet: 1.535 ± 0.035	1.836ArgAsn: 1.836 ± 0.049	1.115ArgPro: 1.115 ± 0.041	1.644ArgGln: 1.644 ± 0.04	1.966ArgArg: 1.966 ± 0.053	2.055ArgSer: 2.055 ± 0.046	2.037ArgThr: 2.037 ± 0.047	2.553ArgVal: 2.553 ± 0.053	0.325ArgTrp: 0.325 ± 0.02	1.817ArgTyr: 1.817 ± 0.045	0.001ArgXaa: 0.001 ± 0.001
Ser	4.258SerAla: 4.258 ± 0.073	0.834SerCys: 0.834 ± 0.031	3.659SerAsp: 3.659 ± 0.07	3.941SerGlu: 3.941 ± 0.071	2.588SerPhe: 2.588 ± 0.051	4.991SerGly: 4.991 ± 0.086	0.986SerHis: 0.986 ± 0.034	3.971SerIle: 3.971 ± 0.073	3.194SerLys: 3.194 ± 0.061	4.714SerLeu: 4.714 ± 0.072	1.901SerMet: 1.901 ± 0.044	2.255SerAsn: 2.255 ± 0.06	1.435SerPro: 1.435 ± 0.039	1.721SerGln: 1.721 ± 0.045	2.275SerArg: 2.275 ± 0.049	3.429SerSer: 3.429 ± 0.08	2.817SerThr: 2.817 ± 0.07	4.344SerVal: 4.344 ± 0.068	0.53SerTrp: 0.53 ± 0.023	2.731SerTyr: 2.731 ± 0.059	0.001SerXaa: 0.001 ± 0.001
Thr	4.727ThrAla: 4.727 ± 0.088	0.684ThrCys: 0.684 ± 0.029	3.677ThrAsp: 3.677 ± 0.065	4.501ThrGlu: 4.501 ± 0.104	2.247ThrPhe: 2.247 ± 0.05	4.888ThrGly: 4.888 ± 0.08	0.867ThrHis: 0.867 ± 0.03	3.959ThrIle: 3.959 ± 0.081	3.304ThrLys: 3.304 ± 0.071	4.776ThrLeu: 4.776 ± 0.077	1.558ThrMet: 1.558 ± 0.042	2.069ThrAsn: 2.069 ± 0.051	1.969ThrPro: 1.969 ± 0.049	1.543ThrGln: 1.543 ± 0.048	1.748ThrArg: 1.748 ± 0.042	3.06ThrSer: 3.06 ± 0.075	2.951ThrThr: 2.951 ± 0.076	4.77ThrVal: 4.77 ± 0.091	0.526ThrTrp: 0.526 ± 0.025	2.3ThrTyr: 2.3 ± 0.058	0.001ThrXaa: 0.001 ± 0.001
Val	4.973ValAla: 4.973 ± 0.101	1.307ValCys: 1.307 ± 0.042	3.823ValAsp: 3.823 ± 0.074	4.641ValGlu: 4.641 ± 0.073	3.05ValPhe: 3.05 ± 0.069	4.209ValGly: 4.209 ± 0.087	1.081ValHis: 1.081 ± 0.037	5.407ValIle: 5.407 ± 0.079	4.648ValLys: 4.648 ± 0.09	6.661ValLeu: 6.661 ± 0.103	2.167ValMet: 2.167 ± 0.043	3.09ValAsn: 3.09 ± 0.063	2.428ValPro: 2.428 ± 0.053	1.894ValGln: 1.894 ± 0.05	2.902ValArg: 2.902 ± 0.053	4.753ValSer: 4.753 ± 0.084	4.753ValThr: 4.753 ± 0.084	4.935ValVal: 4.935 ± 0.083	0.683ValTrp: 0.683 ± 0.028	2.935ValTyr: 2.935 ± 0.057	0.001ValXaa: 0.001 ± 0.001
Trp	0.518TrpAla: 0.518 ± 0.028	0.174TrpCys: 0.174 ± 0.014	0.591TrpAsp: 0.591 ± 0.027	0.72TrpGlu: 0.72 ± 0.029	0.416TrpPhe: 0.416 ± 0.022	0.603TrpGly: 0.603 ± 0.03	0.168TrpHis: 0.168 ± 0.012	0.656TrpIle: 0.656 ± 0.027	0.773TrpLys: 0.773 ± 0.029	0.784TrpLeu: 0.784 ± 0.033	0.295TrpMet: 0.295 ± 0.017	0.613TrpAsn: 0.613 ± 0.029	0.178TrpPro: 0.178 ± 0.013	0.344TrpGln: 0.344 ± 0.017	0.36TrpArg: 0.36 ± 0.021	0.493TrpSer: 0.493 ± 0.024	0.428TrpThr: 0.428 ± 0.021	0.479TrpVal: 0.479 ± 0.028	0.131TrpTrp: 0.131 ± 0.014	0.436TrpTyr: 0.436 ± 0.024	0.002TrpXaa: 0.002 ± 0.002
Tyr	2.988TyrAla: 2.988 ± 0.056	0.597TyrCys: 0.597 ± 0.027	2.709TyrAsp: 2.709 ± 0.057	3.281TyrGlu: 3.281 ± 0.072	1.941TyrPhe: 1.941 ± 0.047	3.04TyrGly: 3.04 ± 0.067	0.968TyrHis: 0.968 ± 0.033	2.816TyrIle: 2.816 ± 0.053	2.277TyrLys: 2.277 ± 0.056	3.923TyrLeu: 3.923 ± 0.079	1.183TyrMet: 1.183 ± 0.044	1.899TyrAsn: 1.899 ± 0.05	1.465TyrPro: 1.465 ± 0.044	1.846TyrGln: 1.846 ± 0.042	2.174TyrArg: 2.174 ± 0.056	2.255TyrSer: 2.255 ± 0.049	2.396TyrThr: 2.396 ± 0.053	2.823TyrVal: 2.823 ± 0.057	0.388TyrTrp: 0.388 ± 0.021	2.108TyrTyr: 2.108 ± 0.065	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.001XaaAla: 0.001 ± 0.001	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.001XaaGlu: 0.001 ± 0.001	0.003XaaPhe: 0.003 ± 0.002	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.002XaaIle: 0.002 ± 0.001	0.0XaaLys: 0.0 ± 0.0	0.001XaaLeu: 0.001 ± 0.001	0.002XaaMet: 0.002 ± 0.001	0.0XaaAsn: 0.0 ± 0.0	0.004XaaPro: 0.004 ± 0.002	0.001XaaGln: 0.001 ± 0.001	0.002XaaArg: 0.002 ± 0.002	0.0XaaSer: 0.0 ± 0.0	0.002XaaThr: 0.002 ± 0.001	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.02XaaXaa: 0.02 ± 0.007

Statistics based on 2856 proteins (948991 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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