Amino acid dipepetide frequency for Chania multitudinisentens RB-25

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	10.571AlaAla: 10.571 ± 0.106	1.133AlaCys: 1.133 ± 0.031	4.824AlaAsp: 4.824 ± 0.062	6.063AlaGlu: 6.063 ± 0.092	3.538AlaPhe: 3.538 ± 0.05	7.714AlaGly: 7.714 ± 0.102	1.832AlaHis: 1.832 ± 0.039	5.964AlaIle: 5.964 ± 0.078	3.854AlaLys: 3.854 ± 0.059	12.022AlaLeu: 12.022 ± 0.125	2.845AlaMet: 2.845 ± 0.049	3.435AlaAsn: 3.435 ± 0.061	3.642AlaPro: 3.642 ± 0.059	4.823AlaGln: 4.823 ± 0.08	5.063AlaArg: 5.063 ± 0.081	5.409AlaSer: 5.409 ± 0.08	4.925AlaThr: 4.925 ± 0.075	6.778AlaVal: 6.778 ± 0.081	1.292AlaTrp: 1.292 ± 0.029	2.408AlaTyr: 2.408 ± 0.046	0.0AlaXaa: 0.0 ± 0.0
Cys	0.96CysAla: 0.96 ± 0.025	0.153CysCys: 0.153 ± 0.012	0.57CysAsp: 0.57 ± 0.022	0.558CysGlu: 0.558 ± 0.023	0.433CysPhe: 0.433 ± 0.019	1.035CysGly: 1.035 ± 0.033	0.336CysHis: 0.336 ± 0.017	0.566CysIle: 0.566 ± 0.019	0.323CysLys: 0.323 ± 0.016	1.089CysLeu: 1.089 ± 0.029	0.257CysMet: 0.257 ± 0.014	0.336CysAsn: 0.336 ± 0.015	0.481CysPro: 0.481 ± 0.022	0.527CysGln: 0.527 ± 0.02	0.61CysArg: 0.61 ± 0.021	0.662CysSer: 0.662 ± 0.024	0.52CysThr: 0.52 ± 0.02	0.716CysVal: 0.716 ± 0.021	0.197CysTrp: 0.197 ± 0.013	0.323CysTyr: 0.323 ± 0.014	0.0CysXaa: 0.0 ± 0.0
Asp	4.646AspAla: 4.646 ± 0.062	0.531AspCys: 0.531 ± 0.021	2.641AspAsp: 2.641 ± 0.062	3.195AspGlu: 3.195 ± 0.06	2.146AspPhe: 2.146 ± 0.036	3.681AspGly: 3.681 ± 0.063	0.97AspHis: 0.97 ± 0.024	3.349AspIle: 3.349 ± 0.054	2.202AspLys: 2.202 ± 0.039	4.662AspLeu: 4.662 ± 0.069	1.196AspMet: 1.196 ± 0.026	2.158AspAsn: 2.158 ± 0.058	2.055AspPro: 2.055 ± 0.039	1.645AspGln: 1.645 ± 0.036	2.615AspArg: 2.615 ± 0.045	2.834AspSer: 2.834 ± 0.044	2.415AspThr: 2.415 ± 0.045	3.475AspVal: 3.475 ± 0.057	0.825AspTrp: 0.825 ± 0.024	1.833AspTyr: 1.833 ± 0.036	0.0AspXaa: 0.0 ± 0.0
Glu	5.012GluAla: 5.012 ± 0.077	0.459GluCys: 0.459 ± 0.019	2.196GluAsp: 2.196 ± 0.046	3.021GluGlu: 3.021 ± 0.063	1.824GluPhe: 1.824 ± 0.039	3.382GluGly: 3.382 ± 0.056	1.487GluHis: 1.487 ± 0.032	3.271GluIle: 3.271 ± 0.054	2.826GluLys: 2.826 ± 0.051	6.202GluLeu: 6.202 ± 0.074	1.701GluMet: 1.701 ± 0.037	2.304GluAsn: 2.304 ± 0.033	2.161GluPro: 2.161 ± 0.039	4.018GluGln: 4.018 ± 0.066	3.669GluArg: 3.669 ± 0.061	2.785GluSer: 2.785 ± 0.052	2.726GluThr: 2.726 ± 0.05	3.635GluVal: 3.635 ± 0.06	0.725GluTrp: 0.725 ± 0.025	1.435GluTyr: 1.435 ± 0.035	0.0GluXaa: 0.0 ± 0.0
Phe	3.679PheAla: 3.679 ± 0.057	0.517PheCys: 0.517 ± 0.021	2.324PheAsp: 2.324 ± 0.05	1.744PheGlu: 1.744 ± 0.037	1.639PhePhe: 1.639 ± 0.042	3.148PheGly: 3.148 ± 0.05	0.798PheHis: 0.798 ± 0.025	2.589PheIle: 2.589 ± 0.049	1.303PheLys: 1.303 ± 0.031	3.258PheLeu: 3.258 ± 0.053	0.944PheMet: 0.944 ± 0.025	1.877PheAsn: 1.877 ± 0.04	1.46PhePro: 1.46 ± 0.033	1.179PheGln: 1.179 ± 0.029	1.841PheArg: 1.841 ± 0.034	3.206PheSer: 3.206 ± 0.049	2.484PheThr: 2.484 ± 0.058	2.344PheVal: 2.344 ± 0.048	0.554PheTrp: 0.554 ± 0.023	1.28PheTyr: 1.28 ± 0.029	0.0PheXaa: 0.0 ± 0.0
Gly	6.454GlyAla: 6.454 ± 0.121	0.99GlyCys: 0.99 ± 0.027	3.486GlyAsp: 3.486 ± 0.059	4.409GlyGlu: 4.409 ± 0.071	3.156GlyPhe: 3.156 ± 0.051	5.464GlyGly: 5.464 ± 0.126	1.631GlyHis: 1.631 ± 0.032	5.142GlyIle: 5.142 ± 0.069	3.812GlyLys: 3.812 ± 0.062	7.44GlyLeu: 7.44 ± 0.083	2.264GlyMet: 2.264 ± 0.045	3.047GlyAsn: 3.047 ± 0.096	2.053GlyPro: 2.053 ± 0.04	3.228GlyGln: 3.228 ± 0.052	3.751GlyArg: 3.751 ± 0.057	4.669GlySer: 4.669 ± 0.137	3.883GlyThr: 3.883 ± 0.11	5.719GlyVal: 5.719 ± 0.068	1.258GlyTrp: 1.258 ± 0.03	2.751GlyTyr: 2.751 ± 0.052	0.0GlyXaa: 0.0 ± 0.0
His	1.874HisAla: 1.874 ± 0.041	0.353HisCys: 0.353 ± 0.016	1.102HisAsp: 1.102 ± 0.029	1.066HisGlu: 1.066 ± 0.028	1.061HisPhe: 1.061 ± 0.029	1.715HisGly: 1.715 ± 0.034	0.801HisHis: 0.801 ± 0.03	1.298HisIle: 1.298 ± 0.029	0.71HisLys: 0.71 ± 0.022	2.36HisLeu: 2.36 ± 0.046	0.511HisMet: 0.511 ± 0.018	0.838HisAsn: 0.838 ± 0.023	1.344HisPro: 1.344 ± 0.029	1.384HisGln: 1.384 ± 0.033	1.263HisArg: 1.263 ± 0.03	1.349HisSer: 1.349 ± 0.029	1.072HisThr: 1.072 ± 0.027	1.169HisVal: 1.169 ± 0.033	0.436HisTrp: 0.436 ± 0.02	0.954HisTyr: 0.954 ± 0.026	0.0HisXaa: 0.0 ± 0.0
Ile	6.379IleAla: 6.379 ± 0.074	0.651IleCys: 0.651 ± 0.02	3.458IleAsp: 3.458 ± 0.051	3.447IleGlu: 3.447 ± 0.057	2.042IlePhe: 2.042 ± 0.048	4.865IleGly: 4.865 ± 0.071	1.175IleHis: 1.175 ± 0.033	3.276IleIle: 3.276 ± 0.07	2.395IleLys: 2.395 ± 0.048	5.083IleLeu: 5.083 ± 0.075	1.216IleMet: 1.216 ± 0.035	2.708IleAsn: 2.708 ± 0.047	2.859IlePro: 2.859 ± 0.047	2.057IleGln: 2.057 ± 0.036	2.938IleArg: 2.938 ± 0.049	4.001IleSer: 4.001 ± 0.062	3.751IleThr: 3.751 ± 0.069	3.832IleVal: 3.832 ± 0.067	0.631IleTrp: 0.631 ± 0.024	1.624IleTyr: 1.624 ± 0.035	0.0IleXaa: 0.0 ± 0.0
Lys	3.774LysAla: 3.774 ± 0.066	0.247LysCys: 0.247 ± 0.015	1.822LysAsp: 1.822 ± 0.044	2.211LysGlu: 2.211 ± 0.05	1.07LysPhe: 1.07 ± 0.031	2.72LysGly: 2.72 ± 0.056	0.877LysHis: 0.877 ± 0.027	2.314LysIle: 2.314 ± 0.044	2.086LysLys: 2.086 ± 0.05	4.275LysLeu: 4.275 ± 0.063	1.124LysMet: 1.124 ± 0.028	1.739LysAsn: 1.739 ± 0.038	2.077LysPro: 2.077 ± 0.043	2.217LysGln: 2.217 ± 0.045	2.422LysArg: 2.422 ± 0.047	2.133LysSer: 2.133 ± 0.042	2.429LysThr: 2.429 ± 0.045	2.92LysVal: 2.92 ± 0.051	0.405LysTrp: 0.405 ± 0.021	1.044LysTyr: 1.044 ± 0.031	0.0LysXaa: 0.0 ± 0.0
Leu	12.297LeuAla: 12.297 ± 0.106	1.317LeuCys: 1.317 ± 0.032	5.337LeuAsp: 5.337 ± 0.072	5.585LeuGlu: 5.585 ± 0.075	4.264LeuPhe: 4.264 ± 0.067	7.682LeuGly: 7.682 ± 0.098	2.326LeuHis: 2.326 ± 0.049	5.812LeuIle: 5.812 ± 0.086	4.371LeuLys: 4.371 ± 0.069	12.879LeuLeu: 12.879 ± 0.149	2.961LeuMet: 2.961 ± 0.05	4.546LeuAsn: 4.546 ± 0.082	6.061LeuPro: 6.061 ± 0.082	5.019LeuGln: 5.019 ± 0.077	6.115LeuArg: 6.115 ± 0.078	7.547LeuSer: 7.547 ± 0.096	6.322LeuThr: 6.322 ± 0.111	7.301LeuVal: 7.301 ± 0.086	1.335LeuTrp: 1.335 ± 0.035	2.684LeuTyr: 2.684 ± 0.047	0.0LeuXaa: 0.0 ± 0.0
Met	2.899MetAla: 2.899 ± 0.048	0.199MetCys: 0.199 ± 0.012	1.084MetAsp: 1.084 ± 0.027	1.153MetGlu: 1.153 ± 0.028	0.857MetPhe: 0.857 ± 0.026	1.791MetGly: 1.791 ± 0.035	0.509MetHis: 0.509 ± 0.018	1.461MetIle: 1.461 ± 0.036	1.268MetLys: 1.268 ± 0.03	3.165MetLeu: 3.165 ± 0.056	0.858MetMet: 0.858 ± 0.027	1.079MetAsn: 1.079 ± 0.027	1.323MetPro: 1.323 ± 0.031	1.289MetGln: 1.289 ± 0.032	1.413MetArg: 1.413 ± 0.035	1.717MetSer: 1.717 ± 0.038	1.578MetThr: 1.578 ± 0.033	1.857MetVal: 1.857 ± 0.038	0.243MetTrp: 0.243 ± 0.013	0.502MetTyr: 0.502 ± 0.02	0.0MetXaa: 0.0 ± 0.0
Asn	3.699AsnAla: 3.699 ± 0.071	0.325AsnCys: 0.325 ± 0.015	1.986AsnAsp: 1.986 ± 0.045	1.959AsnGlu: 1.959 ± 0.04	1.3AsnPhe: 1.3 ± 0.032	3.259AsnGly: 3.259 ± 0.085	0.954AsnHis: 0.954 ± 0.029	2.501AsnIle: 2.501 ± 0.039	1.51AsnLys: 1.51 ± 0.037	3.816AsnLeu: 3.816 ± 0.052	0.915AsnMet: 0.915 ± 0.026	1.804AsnAsn: 1.804 ± 0.052	2.102AsnPro: 2.102 ± 0.04	1.92AsnGln: 1.92 ± 0.037	2.081AsnArg: 2.081 ± 0.04	2.333AsnSer: 2.333 ± 0.061	2.26AsnThr: 2.26 ± 0.058	2.623AsnVal: 2.623 ± 0.054	0.561AsnTrp: 0.561 ± 0.02	1.25AsnTyr: 1.25 ± 0.032	0.0AsnXaa: 0.0 ± 0.0
Pro	4.649ProAla: 4.649 ± 0.063	0.388ProCys: 0.388 ± 0.016	2.432ProAsp: 2.432 ± 0.044	3.245ProGlu: 3.245 ± 0.053	1.849ProPhe: 1.849 ± 0.038	3.315ProGly: 3.315 ± 0.054	1.037ProHis: 1.037 ± 0.028	2.197ProIle: 2.197 ± 0.04	1.529ProLys: 1.529 ± 0.035	5.436ProLeu: 5.436 ± 0.076	1.083ProMet: 1.083 ± 0.031	1.467ProAsn: 1.467 ± 0.031	1.789ProPro: 1.789 ± 0.043	2.493ProGln: 2.493 ± 0.043	1.877ProArg: 1.877 ± 0.043	2.33ProSer: 2.33 ± 0.045	2.313ProThr: 2.313 ± 0.052	3.555ProVal: 3.555 ± 0.048	0.654ProTrp: 0.654 ± 0.024	1.366ProTyr: 1.366 ± 0.034	0.0ProXaa: 0.0 ± 0.0
Gln	5.363GlnAla: 5.363 ± 0.07	0.396GlnCys: 0.396 ± 0.019	2.067GlnAsp: 2.067 ± 0.042	2.419GlnGlu: 2.419 ± 0.044	1.574GlnPhe: 1.574 ± 0.038	3.64GlnGly: 3.64 ± 0.054	1.426GlnHis: 1.426 ± 0.035	2.529GlnIle: 2.529 ± 0.044	1.754GlnLys: 1.754 ± 0.036	5.844GlnLeu: 5.844 ± 0.079	1.273GlnMet: 1.273 ± 0.03	1.551GlnAsn: 1.551 ± 0.033	2.556GlnPro: 2.556 ± 0.05	4.444GlnGln: 4.444 ± 0.095	3.617GlnArg: 3.617 ± 0.067	2.393GlnSer: 2.393 ± 0.043	2.29GlnThr: 2.29 ± 0.044	3.452GlnVal: 3.452 ± 0.056	0.736GlnTrp: 0.736 ± 0.023	1.211GlnTyr: 1.211 ± 0.032	0.0GlnXaa: 0.0 ± 0.0
Arg	4.522ArgAla: 4.522 ± 0.067	0.62ArgCys: 0.62 ± 0.024	2.749ArgAsp: 2.749 ± 0.049	3.431ArgGlu: 3.431 ± 0.06	2.582ArgPhe: 2.582 ± 0.043	3.308ArgGly: 3.308 ± 0.054	1.484ArgHis: 1.484 ± 0.033	3.373ArgIle: 3.373 ± 0.056	2.103ArgLys: 2.103 ± 0.043	6.612ArgLeu: 6.612 ± 0.09	1.455ArgMet: 1.455 ± 0.032	2.052ArgAsn: 2.052 ± 0.04	2.122ArgPro: 2.122 ± 0.037	3.214ArgGln: 3.214 ± 0.055	3.251ArgArg: 3.251 ± 0.057	2.906ArgSer: 2.906 ± 0.047	2.382ArgThr: 2.382 ± 0.04	3.718ArgVal: 3.718 ± 0.048	0.927ArgTrp: 0.927 ± 0.029	2.141ArgTyr: 2.141 ± 0.04	0.0ArgXaa: 0.0 ± 0.0
Ser	5.713SerAla: 5.713 ± 0.078	0.583SerCys: 0.583 ± 0.02	2.939SerAsp: 2.939 ± 0.057	3.093SerGlu: 3.093 ± 0.047	2.369SerPhe: 2.369 ± 0.041	5.564SerGly: 5.564 ± 0.157	1.439SerHis: 1.439 ± 0.029	3.257SerIle: 3.257 ± 0.059	2.133SerLys: 2.133 ± 0.038	6.818SerLeu: 6.818 ± 0.079	1.492SerMet: 1.492 ± 0.033	2.192SerAsn: 2.192 ± 0.054	2.617SerPro: 2.617 ± 0.04	2.77SerGln: 2.77 ± 0.042	3.1SerArg: 3.1 ± 0.051	3.773SerSer: 3.773 ± 0.061	3.136SerThr: 3.136 ± 0.071	4.301SerVal: 4.301 ± 0.069	0.872SerTrp: 0.872 ± 0.023	1.813SerTyr: 1.813 ± 0.046	0.0SerXaa: 0.0 ± 0.0
Thr	5.173ThrAla: 5.173 ± 0.082	0.469ThrCys: 0.469 ± 0.018	2.497ThrAsp: 2.497 ± 0.075	2.679ThrGlu: 2.679 ± 0.046	2.031ThrPhe: 2.031 ± 0.046	4.266ThrGly: 4.266 ± 0.081	1.204ThrHis: 1.204 ± 0.03	2.888ThrIle: 2.888 ± 0.048	1.486ThrLys: 1.486 ± 0.038	7.651ThrLeu: 7.651 ± 0.149	1.073ThrMet: 1.073 ± 0.026	1.681ThrAsn: 1.681 ± 0.048	3.293ThrPro: 3.293 ± 0.074	2.405ThrGln: 2.405 ± 0.041	2.758ThrArg: 2.758 ± 0.045	2.958ThrSer: 2.958 ± 0.06	3.006ThrThr: 3.006 ± 0.085	4.01ThrVal: 4.01 ± 0.106	0.691ThrTrp: 0.691 ± 0.025	1.336ThrTyr: 1.336 ± 0.034	0.0ThrXaa: 0.0 ± 0.0
Val	6.951ValAla: 6.951 ± 0.084	0.764ValCys: 0.764 ± 0.026	3.503ValAsp: 3.503 ± 0.056	3.893ValGlu: 3.893 ± 0.063	2.605ValPhe: 2.605 ± 0.047	4.87ValGly: 4.87 ± 0.08	1.205ValHis: 1.205 ± 0.034	4.506ValIle: 4.506 ± 0.067	2.951ValLys: 2.951 ± 0.054	7.549ValLeu: 7.549 ± 0.075	2.122ValMet: 2.122 ± 0.038	2.826ValAsn: 2.826 ± 0.047	2.889ValPro: 2.889 ± 0.048	2.63ValGln: 2.63 ± 0.049	3.53ValArg: 3.53 ± 0.05	4.444ValSer: 4.444 ± 0.059	4.104ValThr: 4.104 ± 0.083	5.366ValVal: 5.366 ± 0.07	0.835ValTrp: 0.835 ± 0.026	1.794ValTyr: 1.794 ± 0.041	0.0ValXaa: 0.0 ± 0.0
Trp	1.02TrpAla: 1.02 ± 0.027	0.177TrpCys: 0.177 ± 0.011	0.588TrpAsp: 0.588 ± 0.022	0.552TrpGlu: 0.552 ± 0.021	0.605TrpPhe: 0.605 ± 0.024	0.908TrpGly: 0.908 ± 0.026	0.383TrpHis: 0.383 ± 0.016	0.64TrpIle: 0.64 ± 0.022	0.425TrpLys: 0.425 ± 0.018	2.208TrpLeu: 2.208 ± 0.053	0.373TrpMet: 0.373 ± 0.018	0.449TrpAsn: 0.449 ± 0.02	0.617TrpPro: 0.617 ± 0.019	1.279TrpGln: 1.279 ± 0.033	1.009TrpArg: 1.009 ± 0.03	0.75TrpSer: 0.75 ± 0.027	0.438TrpThr: 0.438 ± 0.021	0.875TrpVal: 0.875 ± 0.028	0.239TrpTrp: 0.239 ± 0.013	0.383TrpTyr: 0.383 ± 0.019	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.498TyrAla: 2.498 ± 0.041	0.381TyrCys: 0.381 ± 0.017	1.468TyrAsp: 1.468 ± 0.037	1.118TyrGlu: 1.118 ± 0.028	1.221TyrPhe: 1.221 ± 0.032	2.276TyrGly: 2.276 ± 0.045	0.787TyrHis: 0.787 ± 0.021	1.457TyrIle: 1.457 ± 0.034	0.864TyrLys: 0.864 ± 0.029	3.394TyrLeu: 3.394 ± 0.059	0.583TyrMet: 0.583 ± 0.02	1.071TyrAsn: 1.071 ± 0.032	1.505TyrPro: 1.505 ± 0.036	1.992TyrGln: 1.992 ± 0.036	2.026TyrArg: 2.026 ± 0.044	1.775TyrSer: 1.775 ± 0.041	1.52TyrThr: 1.52 ± 0.04	1.691TyrVal: 1.691 ± 0.037	0.505TyrTrp: 0.505 ± 0.019	0.995TyrTyr: 0.995 ± 0.029	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4521 proteins (1460264 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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