Pacific flying fox faeces associated gemycircularvirus-6
Average proteome isoelectric point is 6.57
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A140CTM8|A0A140CTM8_9VIRU RepA OS=Pacific flying fox faeces associated gemycircularvirus-6 OX=1795998 PE=4 SV=1
MM1 pKa = 6.77 PAKK4 pKa = 10.47 YY5 pKa = 10.07 KK6 pKa = 11.05 LEE8 pKa = 4.3 DD9 pKa = 3.33 EE10 pKa = 4.52 QYY12 pKa = 11.72 FMLTYY17 pKa = 8.22 PTIPDD22 pKa = 3.84 GFDD25 pKa = 3.0 TDD27 pKa = 4.31 ALISVLEE34 pKa = 4.16 RR35 pKa = 11.84 LGCDD39 pKa = 3.23 YY40 pKa = 11.01 RR41 pKa = 11.84 IGRR44 pKa = 11.84 EE45 pKa = 3.75 LHH47 pKa = 6.17 QDD49 pKa = 3.92 GKK51 pKa = 9.48 PHH53 pKa = 5.62 IHH55 pKa = 6.38 VMCCFDD61 pKa = 5.54 DD62 pKa = 5.09 PYY64 pKa = 10.88 TDD66 pKa = 3.37 RR67 pKa = 11.84 DD68 pKa = 3.28 ARR70 pKa = 11.84 ATFKK74 pKa = 10.66 IGTRR78 pKa = 11.84 VPNIRR83 pKa = 11.84 VRR85 pKa = 11.84 RR86 pKa = 11.84 AKK88 pKa = 9.84 PQRR91 pKa = 11.84 GWDD94 pKa = 3.67 YY95 pKa = 10.06 VAKK98 pKa = 10.38 HH99 pKa = 5.99 AGQKK103 pKa = 8.71 EE104 pKa = 3.79 GHH106 pKa = 6.31 YY107 pKa = 10.53 IIGEE111 pKa = 3.99 KK112 pKa = 8.88 GTRR115 pKa = 11.84 PGGDD119 pKa = 3.12 GDD121 pKa = 3.85 GSEE124 pKa = 4.88 RR125 pKa = 11.84 PSNDD129 pKa = 1.97 AWFEE133 pKa = 4.12 IILARR138 pKa = 11.84 TRR140 pKa = 11.84 EE141 pKa = 4.08 EE142 pKa = 4.09 FFDD145 pKa = 3.55 AAARR149 pKa = 11.84 LAPRR153 pKa = 11.84 QLACNFSSLTAYY165 pKa = 10.43 ADD167 pKa = 3.15 WKK169 pKa = 9.31 YY170 pKa = 11.08 RR171 pKa = 11.84 PVNVEE176 pKa = 2.96 WSTPDD181 pKa = 3.34 GEE183 pKa = 4.38 FSVPFEE189 pKa = 4.07 LRR191 pKa = 11.84 DD192 pKa = 3.56 WVDD195 pKa = 3.66 DD196 pKa = 3.92 NVRR199 pKa = 11.84 GCSGMCSPFPTGGRR213 pKa = 11.84 SLPRR217 pKa = 11.84 EE218 pKa = 4.05
Molecular weight: 24.86 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.527
IPC2_protein 5.575
IPC_protein 5.563
Toseland 5.779
ProMoST 5.652
Dawson 5.741
Bjellqvist 5.792
Wikipedia 5.715
Rodwell 5.69
Grimsley 5.855
Solomon 5.741
Lehninger 5.715
Nozaki 5.957
DTASelect 6.148
Thurlkill 6.071
EMBOSS 6.033
Sillero 6.046
Patrickios 4.126
IPC_peptide 5.753
IPC2_peptide 6.059
IPC2.peptide.svr19 6.065
Protein with the highest isoelectric point:
>tr|A0A140CTM7|A0A140CTM7_9VIRU Replication-associated protein OS=Pacific flying fox faeces associated gemycircularvirus-6 OX=1795998 PE=4 SV=1
MM1 pKa = 7.87 PYY3 pKa = 10.37 ARR5 pKa = 11.84 RR6 pKa = 11.84 YY7 pKa = 3.96 TTYY10 pKa = 9.75 RR11 pKa = 11.84 RR12 pKa = 11.84 SYY14 pKa = 9.54 PRR16 pKa = 11.84 RR17 pKa = 11.84 PYY19 pKa = 10.21 QSRR22 pKa = 11.84 RR23 pKa = 11.84 SYY25 pKa = 10.42 GVRR28 pKa = 11.84 RR29 pKa = 11.84 PYY31 pKa = 10.2 YY32 pKa = 8.02 RR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 YY36 pKa = 8.34 FTMPYY41 pKa = 7.56 YY42 pKa = 10.33 SRR44 pKa = 11.84 RR45 pKa = 11.84 RR46 pKa = 11.84 RR47 pKa = 11.84 YY48 pKa = 7.95 GHH50 pKa = 6.17 RR51 pKa = 11.84 RR52 pKa = 11.84 QYY54 pKa = 9.88 GRR56 pKa = 11.84 KK57 pKa = 8.13 RR58 pKa = 11.84 VLNITSRR65 pKa = 11.84 KK66 pKa = 9.15 KK67 pKa = 10.21 QDD69 pKa = 2.9 TMLPFTNTTASAVSGGTTFTNQAAVLVGGNTYY101 pKa = 10.06 VFPWCCTARR110 pKa = 11.84 DD111 pKa = 3.77 NTINGSPATVFATAARR127 pKa = 11.84 TATEE131 pKa = 4.25 CYY133 pKa = 9.36 MRR135 pKa = 11.84 GLKK138 pKa = 9.86 EE139 pKa = 4.23 RR140 pKa = 11.84 IQIQTNTGMPWQWRR154 pKa = 11.84 RR155 pKa = 11.84 ICFTLKK161 pKa = 10.73 GDD163 pKa = 4.02 VLTQYY168 pKa = 11.36 NEE170 pKa = 4.01 TAYY173 pKa = 9.8 KK174 pKa = 10.17 IYY176 pKa = 10.76 DD177 pKa = 3.67 EE178 pKa = 5.09 TSDD181 pKa = 2.97 GWSRR185 pKa = 11.84 IVSNTGVASNLATAVQNLVFAGQQGKK211 pKa = 9.72 DD212 pKa = 2.76 WTAVFNAKK220 pKa = 9.82 VDD222 pKa = 3.87 DD223 pKa = 3.95 RR224 pKa = 11.84 RR225 pKa = 11.84 LTLKK229 pKa = 10.29 YY230 pKa = 10.64 DD231 pKa = 3.02 KK232 pKa = 10.28 TVYY235 pKa = 9.75 IRR237 pKa = 11.84 SGNASGVMRR246 pKa = 11.84 DD247 pKa = 3.24 YY248 pKa = 11.31 HH249 pKa = 6.47 RR250 pKa = 11.84 WHH252 pKa = 7.21 PMNANLVYY260 pKa = 10.79 DD261 pKa = 4.6 DD262 pKa = 5.69 DD263 pKa = 4.33 EE264 pKa = 7.31 AGTSMVPSYY273 pKa = 9.96 FSPEE277 pKa = 3.19 GRR279 pKa = 11.84 AAMGDD284 pKa = 4.17 YY285 pKa = 10.67 YY286 pKa = 11.48 VLDD289 pKa = 4.93 IIAGGSGATSSDD301 pKa = 2.93 QLTFNPQTTLYY312 pKa = 8.18 WHH314 pKa = 6.53 EE315 pKa = 4.1 RR316 pKa = 3.51
Molecular weight: 36.45 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.567
IPC2_protein 9.794
IPC_protein 10.423
Toseland 9.809
ProMoST 9.882
Dawson 10.189
Bjellqvist 9.999
Wikipedia 10.467
Rodwell 10.248
Grimsley 10.321
Solomon 10.218
Lehninger 10.145
Nozaki 9.853
DTASelect 9.984
Thurlkill 9.984
EMBOSS 10.277
Sillero 10.101
Patrickios 4.584
IPC_peptide 10.189
IPC2_peptide 9.077
IPC2.peptide.svr19 8.484
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
871
218
337
290.3
33.42
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.348 ± 0.265
2.067 ± 0.344
7.692 ± 1.107
4.937 ± 1.147
4.592 ± 0.618
8.266 ± 0.419
1.837 ± 0.243
4.133 ± 0.41
4.363 ± 0.607
5.281 ± 0.373
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.181 ± 0.281
3.559 ± 0.645
5.281 ± 0.682
2.87 ± 0.527
9.874 ± 0.683
5.052 ± 0.549
6.774 ± 1.695
5.396 ± 0.301
2.641 ± 0.278
5.855 ± 1.003
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here