Enterococcus phage vB_EfaS_IME198

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Saphexavirus; unclassified Saphexavirus

Average proteome isoelectric point is 5.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 95 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0S2MXZ2|A0A0S2MXZ2_9CAUD Tail fibers protein OS=Enterococcus phage vB_EfaS_IME198 OX=1747287 PE=4 SV=1
MM1 pKa = 7.82AIYY4 pKa = 9.78FSIKK8 pKa = 8.49VWEE11 pKa = 4.32PVPEE15 pKa = 4.45EE16 pKa = 3.67YY17 pKa = 10.03WNPNEE22 pKa = 4.18YY23 pKa = 10.33YY24 pKa = 10.84LPVDD28 pKa = 3.46YY29 pKa = 10.05TINPSDD35 pKa = 3.95EE36 pKa = 4.19NLLDD40 pKa = 3.84VMEE43 pKa = 5.65DD44 pKa = 3.63LLDD47 pKa = 4.63DD48 pKa = 4.0GCEE51 pKa = 3.86IVSVEE56 pKa = 4.25MFSTLHH62 pKa = 6.6KK63 pKa = 10.61EE64 pKa = 4.43RR65 pKa = 11.84IDD67 pKa = 5.42LPWQDD72 pKa = 3.51YY73 pKa = 8.93VEE75 pKa = 4.66RR76 pKa = 11.84SLL78 pKa = 4.87

Molecular weight: 9.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0S2MXX5|A0A0S2MXX5_9CAUD BIG2 domain-containing protein OS=Enterococcus phage vB_EfaS_IME198 OX=1747287 PE=4 SV=1
MM1 pKa = 6.9NTEE4 pKa = 3.81KK5 pKa = 10.36MKK7 pKa = 10.92SEE9 pKa = 4.16KK10 pKa = 10.04AWNRR14 pKa = 11.84AIVKK18 pKa = 10.21AEE20 pKa = 3.61QEE22 pKa = 4.03AAKK25 pKa = 10.69KK26 pKa = 10.45KK27 pKa = 8.64MEE29 pKa = 4.46RR30 pKa = 11.84LAKK33 pKa = 9.42MRR35 pKa = 11.84AKK37 pKa = 10.47AKK39 pKa = 10.39RR40 pKa = 11.84KK41 pKa = 9.4

Molecular weight: 4.85 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

95

0

95

16630

38

1330

175.1

19.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.246 ± 0.446

0.679 ± 0.09

6.218 ± 0.211

9.206 ± 0.378

4.095 ± 0.241

6.031 ± 0.372

1.461 ± 0.103

6.212 ± 0.186

7.625 ± 0.314

8.611 ± 0.343

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.814 ± 0.168

5.063 ± 0.216

3.001 ± 0.204

3.488 ± 0.265

4.155 ± 0.182

5.863 ± 0.172

5.911 ± 0.308

6.705 ± 0.252

1.305 ± 0.107

4.311 ± 0.249
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski