Bacillus phage PBC5

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Saundersvirus; unclassified Saundersvirus

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 93 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A218KCK3|A0A218KCK3_9CAUD Uncharacterized protein OS=Bacillus phage PBC5 OX=1675030 GN=PBC5_069 PE=4 SV=1
MM1 pKa = 6.86LTVYY5 pKa = 8.95EE6 pKa = 4.56IPWGNSVEE14 pKa = 4.07WGAIFCPMLNKK25 pKa = 10.22EE26 pKa = 4.04VMTYY30 pKa = 8.31YY31 pKa = 11.16AKK33 pKa = 10.65GGKK36 pKa = 9.4PYY38 pKa = 9.58DD39 pKa = 3.41TYY41 pKa = 11.88TNIFVNEE48 pKa = 4.55DD49 pKa = 2.62GDD51 pKa = 3.78MYY53 pKa = 11.21YY54 pKa = 8.92YY55 pKa = 10.71TFDD58 pKa = 3.29QDD60 pKa = 2.86EE61 pKa = 4.93GGWHH65 pKa = 6.83EE66 pKa = 4.19EE67 pKa = 4.09PTWVGNVNEE76 pKa = 4.43KK77 pKa = 11.14EE78 pKa = 4.13EE79 pKa = 4.23GG80 pKa = 3.46

Molecular weight: 9.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A218KCN1|A0A218KCN1_9CAUD Putative ParB-like nuclease domain containing protein OS=Bacillus phage PBC5 OX=1675030 GN=PBC5_087 PE=4 SV=1
MM1 pKa = 7.63IGGFSPFDD9 pKa = 3.61NFHH12 pKa = 6.75KK13 pKa = 10.41PSAYY17 pKa = 9.9AKK19 pKa = 9.7KK20 pKa = 9.71RR21 pKa = 11.84YY22 pKa = 8.44WFIGFPLIGTFLIAILIVFYY42 pKa = 11.25VGDD45 pKa = 4.11ALHH48 pKa = 6.88YY49 pKa = 9.05ITTPKK54 pKa = 9.43RR55 pKa = 11.84WKK57 pKa = 9.57GRR59 pKa = 11.84KK60 pKa = 8.3RR61 pKa = 3.31

Molecular weight: 7.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

93

0

93

16910

37

1795

181.8

20.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.564 ± 0.482

0.828 ± 0.128

5.813 ± 0.275

7.771 ± 0.569

3.915 ± 0.154

6.842 ± 0.274

1.934 ± 0.2

6.458 ± 0.211

8.119 ± 0.345

7.12 ± 0.277

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.187 ± 0.221

5.559 ± 0.323

3.134 ± 0.169

4.264 ± 0.175

4.619 ± 0.303

4.672 ± 0.314

6.239 ± 0.347

6.552 ± 0.213

1.419 ± 0.194

3.992 ± 0.253
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski