Longjawed orbweaver circular virus 2
Average proteome isoelectric point is 8.23
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A346BPC3|A0A346BPC3_9VIRU Putative capsid protein OS=Longjawed orbweaver circular virus 2 OX=2293295 PE=4 SV=1
MM1 pKa = 7.11 SQVKK5 pKa = 9.68 KK6 pKa = 7.77 WTLTINNPGLEE17 pKa = 4.11 DD18 pKa = 5.72 HH19 pKa = 6.44 LTLWAKK25 pKa = 9.96 DD26 pKa = 3.33 VKK28 pKa = 11.12 NYY30 pKa = 9.53 GFQYY34 pKa = 10.58 EE35 pKa = 4.46 KK36 pKa = 11.14 VNTYY40 pKa = 10.26 HH41 pKa = 5.91 LQGWILFNDD50 pKa = 4.53 RR51 pKa = 11.84 KK52 pKa = 10.39 RR53 pKa = 11.84 LSQVIYY59 pKa = 10.0 ILLVKK64 pKa = 10.24 KK65 pKa = 7.25 MHH67 pKa = 6.92 PAAHH71 pKa = 5.99 WEE73 pKa = 4.18 VMKK76 pKa = 11.05 GSIKK80 pKa = 10.53 QNEE83 pKa = 4.57 DD84 pKa = 3.45 YY85 pKa = 10.7 CSKK88 pKa = 10.71 SEE90 pKa = 4.21 SSVSVYY96 pKa = 8.1 YY97 pKa = 8.8 TNCNSSKK104 pKa = 10.31 ISASEE109 pKa = 3.78 KK110 pKa = 10.06 LKK112 pKa = 11.11 NDD114 pKa = 3.25 IEE116 pKa = 4.97 NDD118 pKa = 3.59 DD119 pKa = 3.88 SLKK122 pKa = 11.03 DD123 pKa = 3.22 IFSNNFVLSCRR134 pKa = 11.84 YY135 pKa = 9.92 HH136 pKa = 7.33 NFITKK141 pKa = 9.06 YY142 pKa = 9.0 QQILQKK148 pKa = 10.49 PRR150 pKa = 11.84 DD151 pKa = 3.82 HH152 pKa = 6.27 EE153 pKa = 4.75 TIGVTITGPTGTGKK167 pKa = 8.68 TGQIRR172 pKa = 11.84 YY173 pKa = 9.87 NYY175 pKa = 9.45 DD176 pKa = 3.01 INDD179 pKa = 4.27 LYY181 pKa = 10.96 FKK183 pKa = 10.63 PHH185 pKa = 5.87 GPWWDD190 pKa = 3.63 GYY192 pKa = 7.54 TNQPVVVFDD201 pKa = 5.16 EE202 pKa = 5.32 FYY204 pKa = 10.04 SWYY207 pKa = 9.76 PYY209 pKa = 11.18 GEE211 pKa = 4.4 LLRR214 pKa = 11.84 VLDD217 pKa = 4.96 RR218 pKa = 11.84 YY219 pKa = 9.03 PLKK222 pKa = 11.15 VPIKK226 pKa = 10.3 GGFVEE231 pKa = 5.14 FNSEE235 pKa = 3.49 IVYY238 pKa = 7.51 FTSNQNWTSWFPNIEE253 pKa = 4.15 CKK255 pKa = 10.39 DD256 pKa = 3.51 ALEE259 pKa = 4.34 RR260 pKa = 11.84 RR261 pKa = 11.84 LKK263 pKa = 10.43 IQINLNNLDD272 pKa = 5.33 KK273 pKa = 11.18 DD274 pKa = 4.36 EE275 pKa = 4.74 CWHH278 pKa = 6.32 GPGVSTSLSRR288 pKa = 11.84 SKK290 pKa = 11.2
Molecular weight: 34.0 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.242
IPC2_protein 7.556
IPC_protein 7.512
Toseland 7.307
ProMoST 8.156
Dawson 8.2
Bjellqvist 8.404
Wikipedia 8.141
Rodwell 8.214
Grimsley 7.351
Solomon 8.317
Lehninger 8.317
Nozaki 8.507
DTASelect 8.244
Thurlkill 8.287
EMBOSS 8.404
Sillero 8.565
Patrickios 4.012
IPC_peptide 8.302
IPC2_peptide 7.351
IPC2.peptide.svr19 7.612
Protein with the highest isoelectric point:
>tr|A0A346BPC3|A0A346BPC3_9VIRU Putative capsid protein OS=Longjawed orbweaver circular virus 2 OX=2293295 PE=4 SV=1
MM1 pKa = 7.56 ALLRR5 pKa = 11.84 RR6 pKa = 11.84 RR7 pKa = 11.84 KK8 pKa = 8.08 YY9 pKa = 9.93 NRR11 pKa = 11.84 RR12 pKa = 11.84 IRR14 pKa = 11.84 TSWNTGAKK22 pKa = 8.09 TRR24 pKa = 11.84 RR25 pKa = 11.84 TYY27 pKa = 8.97 STNYY31 pKa = 9.07 KK32 pKa = 9.09 YY33 pKa = 10.43 RR34 pKa = 11.84 PRR36 pKa = 11.84 RR37 pKa = 11.84 RR38 pKa = 11.84 RR39 pKa = 11.84 TYY41 pKa = 10.18 RR42 pKa = 11.84 YY43 pKa = 6.92 TKK45 pKa = 10.3 SKK47 pKa = 9.91 PFTYY51 pKa = 9.85 GKK53 pKa = 9.96 RR54 pKa = 11.84 YY55 pKa = 8.71 RR56 pKa = 11.84 KK57 pKa = 9.49 RR58 pKa = 11.84 RR59 pKa = 11.84 IINSLSLPAKK69 pKa = 10.21 KK70 pKa = 10.56 VITKK74 pKa = 8.48 TKK76 pKa = 9.64 HH77 pKa = 4.6 IHH79 pKa = 6.88 DD80 pKa = 3.87 ICLLVDD86 pKa = 4.14 RR87 pKa = 11.84 NGSAASSIDD96 pKa = 3.38 LKK98 pKa = 10.97 AIKK101 pKa = 8.55 WWFPSDD107 pKa = 3.78 DD108 pKa = 4.0 GNKK111 pKa = 9.38 KK112 pKa = 8.55 RR113 pKa = 11.84 QKK115 pKa = 10.64 AIWDD119 pKa = 3.63 KK120 pKa = 11.37 YY121 pKa = 7.72 EE122 pKa = 3.69 SHH124 pKa = 7.27 RR125 pKa = 11.84 IKK127 pKa = 10.64 SWTIYY132 pKa = 10.14 IKK134 pKa = 11.11 YY135 pKa = 9.19 KK136 pKa = 10.37 GCSATSIVKK145 pKa = 8.64 TAGATNDD152 pKa = 3.13 QMIPVSVPNFGNEE165 pKa = 4.09 NIDD168 pKa = 3.19 IGVWNRR174 pKa = 11.84 DD175 pKa = 3.63 EE176 pKa = 4.38 IDD178 pKa = 3.65 EE179 pKa = 4.4 VPASGEE185 pKa = 4.17 AYY187 pKa = 10.47 LDD189 pKa = 3.42 AYY191 pKa = 10.08 KK192 pKa = 10.89 YY193 pKa = 10.86 VLNNRR198 pKa = 11.84 KK199 pKa = 8.42 KK200 pKa = 7.81 TLKK203 pKa = 9.9 FHH205 pKa = 6.33 RR206 pKa = 11.84 TFPGMKK212 pKa = 9.15 WFGQGYY218 pKa = 7.86 DD219 pKa = 3.84 TISKK223 pKa = 10.33 SEE225 pKa = 3.83 NSFNFYY231 pKa = 9.34 GTGPAAYY238 pKa = 8.39 TGSIAPIWWKK248 pKa = 10.34 EE249 pKa = 3.65 GKK251 pKa = 9.57 QEE253 pKa = 4.45 FKK255 pKa = 10.37 LTEE258 pKa = 3.66 PTFRR262 pKa = 11.84 FQIDD266 pKa = 3.87 TPNEE270 pKa = 3.5 PDD272 pKa = 3.07 IKK274 pKa = 10.89 QKK276 pKa = 10.98 VLLSKK281 pKa = 10.77 NYY283 pKa = 10.67 SEE285 pKa = 4.74 DD286 pKa = 3.86 CKK288 pKa = 10.57 ITTIWKK294 pKa = 10.35 ANIKK298 pKa = 10.0 LYY300 pKa = 10.65 AYY302 pKa = 7.45 ATWEE306 pKa = 4.13 HH307 pKa = 6.36 GGPRR311 pKa = 11.84 YY312 pKa = 9.98 SVSS315 pKa = 2.96
Molecular weight: 36.95 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.214
IPC2_protein 9.633
IPC_protein 9.721
Toseland 10.233
ProMoST 9.897
Dawson 10.409
Bjellqvist 10.072
Wikipedia 10.584
Rodwell 10.906
Grimsley 10.482
Solomon 10.438
Lehninger 10.394
Nozaki 10.218
DTASelect 10.072
Thurlkill 10.262
EMBOSS 10.628
Sillero 10.321
Patrickios 10.526
IPC_peptide 10.438
IPC2_peptide 8.712
IPC2.peptide.svr19 8.654
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
605
290
315
302.5
35.47
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.967 ± 1.641
1.322 ± 0.294
5.289 ± 0.419
4.463 ± 0.519
3.802 ± 0.246
5.62 ± 0.075
2.149 ± 0.446
7.273 ± 0.527
10.083 ± 1.069
6.116 ± 1.328
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
0.992 ± 0.031
6.777 ± 0.844
4.298 ± 0.117
2.975 ± 0.851
5.95 ± 1.83
7.603 ± 0.24
6.777 ± 0.921
4.628 ± 1.155
3.471 ± 0.017
6.446 ± 0.175
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here