Capybara microvirus Cap1_SP_92
Average proteome isoelectric point is 6.63
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W7N6|A0A4P8W7N6_9VIRU Uncharacterized protein OS=Capybara microvirus Cap1_SP_92 OX=2584798 PE=4 SV=1
MM1 pKa = 8.0 PLLQSQISIIEE12 pKa = 3.91 NAFTVITSLTNGKK25 pKa = 10.07 DD26 pKa = 3.21 FVNFVDD32 pKa = 4.48 SLPSGKK38 pKa = 9.85 SCSIVSLNLGRR49 pKa = 11.84 FKK51 pKa = 11.17 LDD53 pKa = 3.3 YY54 pKa = 9.49 TPSAFSPFGVKK65 pKa = 9.8 HH66 pKa = 5.48 VFSDD70 pKa = 3.7 SMSDD74 pKa = 3.95 SSPLSCFSDD83 pKa = 3.83 LSSFRR88 pKa = 11.84 SVDD91 pKa = 3.32 VLPFNDD97 pKa = 3.39
Molecular weight: 10.55 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.633
IPC2_protein 4.685
IPC_protein 4.482
Toseland 4.266
ProMoST 4.469
Dawson 4.507
Bjellqvist 4.749
Wikipedia 4.495
Rodwell 4.317
Grimsley 4.177
Solomon 4.495
Lehninger 4.457
Nozaki 4.635
DTASelect 4.94
Thurlkill 4.342
EMBOSS 4.495
Sillero 4.622
Patrickios 4.024
IPC_peptide 4.482
IPC2_peptide 4.596
IPC2.peptide.svr19 4.621
Protein with the highest isoelectric point:
>tr|A0A4P8W538|A0A4P8W538_9VIRU Uncharacterized protein OS=Capybara microvirus Cap1_SP_92 OX=2584798 PE=4 SV=1
MM1 pKa = 7.09 VKK3 pKa = 9.86 IYY5 pKa = 10.55 SCSGYY10 pKa = 10.08 QYY12 pKa = 11.25 HH13 pKa = 6.61 SFNLLKK19 pKa = 10.62 RR20 pKa = 11.84 LIYY23 pKa = 10.7 NLFSTRR29 pKa = 11.84 DD30 pKa = 3.62 YY31 pKa = 10.81 TDD33 pKa = 3.86 CSPVAGYY40 pKa = 10.95 YY41 pKa = 10.66 DD42 pKa = 3.9 LVYY45 pKa = 10.6 RR46 pKa = 11.84 DD47 pKa = 4.86 DD48 pKa = 3.93 LCIGAFYY55 pKa = 9.85 YY56 pKa = 10.23 IPSSCKK62 pKa = 8.8 FGYY65 pKa = 9.72 VRR67 pKa = 11.84 SSRR70 pKa = 11.84 RR71 pKa = 11.84 FKK73 pKa = 10.9 YY74 pKa = 9.72 VLYY77 pKa = 9.22 HH78 pKa = 5.46 YY79 pKa = 10.42 RR80 pKa = 11.84 VISLL84 pKa = 3.68
Molecular weight: 10.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.858
IPC2_protein 8.829
IPC_protein 8.887
Toseland 8.697
ProMoST 8.887
Dawson 9.151
Bjellqvist 9.209
Wikipedia 9.37
Rodwell 9.165
Grimsley 9.136
Solomon 9.282
Lehninger 9.224
Nozaki 9.136
DTASelect 9.077
Thurlkill 9.033
EMBOSS 9.238
Sillero 9.268
Patrickios 0.362
IPC_peptide 9.253
IPC2_peptide 8.448
IPC2.peptide.svr19 8.109
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
1618
84
695
269.7
30.93
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.45 ± 0.795
1.36 ± 0.656
5.933 ± 0.491
4.265 ± 0.654
5.748 ± 0.836
4.635 ± 0.529
1.916 ± 0.305
5.315 ± 0.353
5.933 ± 0.841
9.085 ± 0.277
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.731 ± 0.225
6.489 ± 0.798
4.326 ± 0.504
4.141 ± 0.667
4.45 ± 0.727
11.063 ± 1.164
4.388 ± 0.258
6.922 ± 0.708
0.927 ± 0.195
6.922 ± 0.982
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here