Parafilimonas terrae
Average proteome isoelectric point is 6.8
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4249 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1I5Y0K7|A0A1I5Y0K7_9BACT Uncharacterized protein OS=Parafilimonas terrae OX=1465490 GN=SAMN05444277_11068 PE=4 SV=1
MM1 pKa = 7.33 KK2 pKa = 9.98 TRR4 pKa = 11.84 LLLFGLSALIVMHH17 pKa = 6.12 THH19 pKa = 6.04 AQSAGDD25 pKa = 3.8 YY26 pKa = 10.08 RR27 pKa = 11.84 SKK29 pKa = 9.75 QTGNWNDD36 pKa = 3.14 AANWEE41 pKa = 4.61 TFNGTNWVAATNYY54 pKa = 7.13 PTATDD59 pKa = 4.15 GEE61 pKa = 4.34 LSIIATTNITCNVTDD76 pKa = 5.69 NIDD79 pKa = 3.39 QLTIEE84 pKa = 4.69 AGGTLTIDD92 pKa = 3.09 NTEE95 pKa = 4.09 NFNLTAGPDD104 pKa = 3.56 DD105 pKa = 5.37 DD106 pKa = 5.34 VDD108 pKa = 4.2 LKK110 pKa = 11.0 IYY112 pKa = 8.0 GTLQWNKK119 pKa = 9.23 GWAGNCGTWEE129 pKa = 4.1 IYY131 pKa = 10.46 SGGIANINCGSYY143 pKa = 9.37 GTGCNTWNIKK153 pKa = 10.19 PGGTMNFIHH162 pKa = 5.57 VQCYY166 pKa = 10.45 INGTTINNEE175 pKa = 3.55 GTINFTPDD183 pKa = 2.86 WEE185 pKa = 4.32 NMYY188 pKa = 10.32 VRR190 pKa = 11.84 SNTPNSPGTIHH201 pKa = 6.62 NKK203 pKa = 9.83 PNGTVNMIKK212 pKa = 9.5 TGNNGRR218 pKa = 11.84 FGIASDD224 pKa = 4.04 GNNGSEE230 pKa = 4.22 NYY232 pKa = 10.24 VKK234 pKa = 10.61 FINEE238 pKa = 3.51 GTLNLNEE245 pKa = 4.24 GTEE248 pKa = 4.33 LYY250 pKa = 10.84 YY251 pKa = 10.8 GGPGQTSSGISFEE264 pKa = 4.21 NTGTINIANNAMLSNAGIFNFNGGNINGTGNALIRR299 pKa = 11.84 QIGYY303 pKa = 9.2 PGIWKK308 pKa = 10.7 SNVPCNLPVNITFEE322 pKa = 4.93 LKK324 pKa = 8.89 TQLGGPAEE332 pKa = 4.16 TTINGKK338 pKa = 9.31 LRR340 pKa = 11.84 FEE342 pKa = 4.5 GRR344 pKa = 11.84 LSNRR348 pKa = 11.84 DD349 pKa = 3.41 YY350 pKa = 11.78 SEE352 pKa = 6.11 DD353 pKa = 3.36 IAKK356 pKa = 10.23 LRR358 pKa = 11.84 LSPTGTAILVSGYY371 pKa = 10.76 LGGAVLTNEE380 pKa = 4.64 GVITMPEE387 pKa = 3.61 NWGSGLNWEE396 pKa = 5.23 GIGKK400 pKa = 9.29 FINKK404 pKa = 9.33 GLLKK408 pKa = 9.85 LTANQTLYY416 pKa = 10.1 LHH418 pKa = 6.78 NYY420 pKa = 5.33 EE421 pKa = 4.55 TEE423 pKa = 3.98 NGDD426 pKa = 4.09 FINEE430 pKa = 4.06 GVVEE434 pKa = 4.56 IGTGGNLLSNTNSGQTNDD452 pKa = 3.26 FFKK455 pKa = 10.37 NTSAGIIKK463 pKa = 10.51 GNGYY467 pKa = 8.56 VQFDD471 pKa = 3.54 AGFSNNGAITPGTSPGILQFNGAQPLSANSTLEE504 pKa = 3.87 IEE506 pKa = 4.7 VKK508 pKa = 10.24 DD509 pKa = 3.72 ASGAGTGHH517 pKa = 7.0 DD518 pKa = 3.3 QLTRR522 pKa = 11.84 SGDD525 pKa = 3.22 LTLAGKK531 pKa = 10.67 LEE533 pKa = 4.48 VISTGNVPDD542 pKa = 4.18 GSYY545 pKa = 10.11 MIISLSSGTISGSFSEE561 pKa = 5.39 LDD563 pKa = 3.31 MPLGYY568 pKa = 8.85 TLTVNSDD575 pKa = 3.52 NVVLNYY581 pKa = 10.69 ASDD584 pKa = 3.62 MDD586 pKa = 5.01 DD587 pKa = 4.02 DD588 pKa = 4.69 GVINEE593 pKa = 4.56 EE594 pKa = 4.2 DD595 pKa = 4.2 CAPTDD600 pKa = 3.49 ATKK603 pKa = 9.43 WQSATLYY610 pKa = 10.2 IDD612 pKa = 4.26 ADD614 pKa = 3.73 GDD616 pKa = 4.13 GYY618 pKa = 11.3 DD619 pKa = 3.78 AGQEE623 pKa = 4.4 TVCYY627 pKa = 9.72 GATVPDD633 pKa = 5.47 GYY635 pKa = 11.23 SEE637 pKa = 4.21 TSNGEE642 pKa = 3.88 DD643 pKa = 5.29 CNDD646 pKa = 3.51 DD647 pKa = 3.73 DD648 pKa = 6.45 ANVHH652 pKa = 6.17 SSQPYY657 pKa = 8.55 YY658 pKa = 11.12 ADD660 pKa = 3.32 NDD662 pKa = 3.73 GDD664 pKa = 4.35 GFGDD668 pKa = 3.89 AANTTSACSSTPPSGYY684 pKa = 8.51 VTNNTDD690 pKa = 5.24 CDD692 pKa = 4.46 DD693 pKa = 4.39 NDD695 pKa = 3.26 NTIYY699 pKa = 10.53 PGAPEE704 pKa = 5.08 LPDD707 pKa = 5.09 GKK709 pKa = 10.73 DD710 pKa = 3.2 NDD712 pKa = 4.51 CDD714 pKa = 4.24 GEE716 pKa = 4.24 TDD718 pKa = 3.51 EE719 pKa = 5.74 GAGTTWYY726 pKa = 10.35 EE727 pKa = 4.18 DD728 pKa = 3.46 ADD730 pKa = 3.85 GDD732 pKa = 4.64 GFGNPDD738 pKa = 2.9 VSQIAVSQPEE748 pKa = 4.99 GYY750 pKa = 10.35 VADD753 pKa = 4.33 NTDD756 pKa = 3.7 CDD758 pKa = 4.14 DD759 pKa = 3.91 TKK761 pKa = 11.46 LLYY764 pKa = 10.58 ADD766 pKa = 4.07 NDD768 pKa = 3.75 GDD770 pKa = 4.2 GFGAGSPVACGVDD783 pKa = 3.23 NDD785 pKa = 4.73 TDD787 pKa = 4.83 CDD789 pKa = 4.72 DD790 pKa = 4.96 NDD792 pKa = 3.68 DD793 pKa = 4.41 TKK795 pKa = 10.53 WQSGLLYY802 pKa = 10.05 IDD804 pKa = 4.7 FDD806 pKa = 4.82 SDD808 pKa = 4.11 NFDD811 pKa = 3.47 AGQAQVCYY819 pKa = 10.09 GAAIPLGYY827 pKa = 10.64 NPISLGQDD835 pKa = 3.47 CNDD838 pKa = 3.53 HH839 pKa = 7.0 DD840 pKa = 4.08 MHH842 pKa = 7.19 VHH844 pKa = 6.12 TEE846 pKa = 3.59 QTYY849 pKa = 8.77 YY850 pKa = 11.37 ADD852 pKa = 3.59 YY853 pKa = 11.19 DD854 pKa = 3.92 GDD856 pKa = 4.41 GFGDD860 pKa = 3.94 PNNSISVCEE869 pKa = 4.47 AVPPMYY875 pKa = 10.63 YY876 pKa = 8.86 VTDD879 pKa = 3.67 NTDD882 pKa = 3.89 CNDD885 pKa = 3.8 NDD887 pKa = 3.36 NTIYY891 pKa = 10.52 PGAPEE896 pKa = 5.08 LPDD899 pKa = 5.09 GKK901 pKa = 10.73 DD902 pKa = 3.2 NDD904 pKa = 4.51 CDD906 pKa = 4.25 GEE908 pKa = 4.32 TDD910 pKa = 3.56 EE911 pKa = 5.09 GTGTTWYY918 pKa = 10.25 EE919 pKa = 4.02 DD920 pKa = 3.53 ADD922 pKa = 3.85 GDD924 pKa = 4.64 GFGNPDD930 pKa = 2.9 VSQIAVSQPEE940 pKa = 4.99 GYY942 pKa = 10.35 VADD945 pKa = 4.33 NTDD948 pKa = 3.7 CDD950 pKa = 4.14 DD951 pKa = 3.91 TKK953 pKa = 11.46 LLYY956 pKa = 10.58 ADD958 pKa = 4.07 NDD960 pKa = 3.75 GDD962 pKa = 4.21 GFGAGSLVACGVDD975 pKa = 4.05 NNSDD979 pKa = 4.01 CNDD982 pKa = 3.56 NDD984 pKa = 3.32 ATIYY988 pKa = 10.44 PGAPEE993 pKa = 4.6 LCDD996 pKa = 4.68 GKK998 pKa = 10.87 DD999 pKa = 3.56 NNCDD1003 pKa = 3.01 GTTDD1007 pKa = 3.74 EE1008 pKa = 5.09 GCGITTSLRR1017 pKa = 11.84 IDD1019 pKa = 3.68 GLAMRR1024 pKa = 11.84 EE1025 pKa = 4.28 GNSGKK1030 pKa = 10.35 RR1031 pKa = 11.84 NLNFVITLNKK1041 pKa = 9.41 KK1042 pKa = 8.93 SNVPITVKK1050 pKa = 10.55 YY1051 pKa = 8.23 RR1052 pKa = 11.84 TTDD1055 pKa = 3.11 GSATAPADD1063 pKa = 3.42 YY1064 pKa = 10.63 VAADD1068 pKa = 3.52 KK1069 pKa = 10.84 TITIPANTRR1078 pKa = 11.84 LRR1080 pKa = 11.84 TINIAINGDD1089 pKa = 3.16 KK1090 pKa = 10.48 DD1091 pKa = 3.65 YY1092 pKa = 11.44 EE1093 pKa = 4.27 PNEE1096 pKa = 4.19 TVKK1099 pKa = 10.86 VEE1101 pKa = 4.83 LYY1103 pKa = 10.6 DD1104 pKa = 4.06 AVNATIAKK1112 pKa = 7.5 STAVGYY1118 pKa = 10.15 ILNDD1122 pKa = 3.43 DD1123 pKa = 4.31 RR1124 pKa = 11.84 KK1125 pKa = 9.26 QSAVNEE1131 pKa = 4.18 EE1132 pKa = 3.97 PFAAKK1137 pKa = 9.82 AASGGKK1143 pKa = 8.09 VTLSPNPASNIITLSGISIGKK1164 pKa = 9.02 HH1165 pKa = 3.53 ITVSDD1170 pKa = 3.19 IKK1172 pKa = 10.99 GRR1174 pKa = 11.84 TLITQISNASQQKK1187 pKa = 10.2 INISNLAAGTYY1198 pKa = 8.16 IVRR1201 pKa = 11.84 YY1202 pKa = 5.05 EE1203 pKa = 4.29 TEE1205 pKa = 4.34 DD1206 pKa = 3.24 GSYY1209 pKa = 11.38 NSLKK1213 pKa = 10.51 FIKK1216 pKa = 10.19 QQ1217 pKa = 3.1
Molecular weight: 129.06 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.747
IPC2_protein 3.821
IPC_protein 3.872
Toseland 3.643
ProMoST 4.037
Dawson 3.872
Bjellqvist 4.024
Wikipedia 3.808
Rodwell 3.694
Grimsley 3.541
Solomon 3.872
Lehninger 3.821
Nozaki 3.973
DTASelect 4.253
Thurlkill 3.694
EMBOSS 3.821
Sillero 3.999
Patrickios 0.68
IPC_peptide 3.859
IPC2_peptide 3.973
IPC2.peptide.svr19 3.865
Protein with the highest isoelectric point:
>tr|A0A1I5XBR7|A0A1I5XBR7_9BACT N-(5'-phosphoribosyl)anthranilate isomerase OS=Parafilimonas terrae OX=1465490 GN=trpF PE=3 SV=1
MM1 pKa = 7.38 KK2 pKa = 9.6 RR3 pKa = 11.84 TFQPHH8 pKa = 3.73 NRR10 pKa = 11.84 RR11 pKa = 11.84 RR12 pKa = 11.84 KK13 pKa = 8.48 SVHH16 pKa = 4.98 GFRR19 pKa = 11.84 KK20 pKa = 10.11 RR21 pKa = 11.84 MQTANGRR28 pKa = 11.84 KK29 pKa = 8.59 VLASRR34 pKa = 11.84 RR35 pKa = 11.84 TKK37 pKa = 10.38 GRR39 pKa = 11.84 HH40 pKa = 5.24 KK41 pKa = 10.35 LTVSDD46 pKa = 3.83 EE47 pKa = 4.56 RR48 pKa = 11.84 KK49 pKa = 10.22 LKK51 pKa = 10.73
Molecular weight: 6.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.328
IPC2_protein 10.701
IPC_protein 12.266
Toseland 12.442
ProMoST 12.925
Dawson 12.442
Bjellqvist 12.427
Wikipedia 12.91
Rodwell 12.223
Grimsley 12.486
Solomon 12.925
Lehninger 12.837
Nozaki 12.442
DTASelect 12.427
Thurlkill 12.442
EMBOSS 12.939
Sillero 12.442
Patrickios 11.959
IPC_peptide 12.939
IPC2_peptide 11.915
IPC2.peptide.svr19 9.077
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4249
0
4249
1483638
25
3665
349.2
39.21
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.731 ± 0.032
0.92 ± 0.013
5.431 ± 0.029
5.525 ± 0.039
4.992 ± 0.028
6.578 ± 0.036
1.903 ± 0.017
7.52 ± 0.036
7.244 ± 0.034
9.02 ± 0.045
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.299 ± 0.019
6.22 ± 0.04
3.78 ± 0.022
3.738 ± 0.023
3.592 ± 0.023
6.209 ± 0.032
5.687 ± 0.047
6.053 ± 0.028
1.273 ± 0.014
4.283 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here