Amino acid dipepetide frequency for Mycobacterium phage Galactic

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.719AlaAla: 12.719 ± 1.648
0.97AlaCys: 0.97 ± 0.25
7.33AlaAsp: 7.33 ± 0.618
7.222AlaGlu: 7.222 ± 0.624
2.641AlaPhe: 2.641 ± 0.403
9.755AlaGly: 9.755 ± 1.249
2.533AlaHis: 2.533 ± 0.379
4.096AlaIle: 4.096 ± 0.477
3.988AlaLys: 3.988 ± 0.45
7.707AlaLeu: 7.707 ± 0.804
2.21AlaMet: 2.21 ± 0.334
2.371AlaAsn: 2.371 ± 0.379
5.12AlaPro: 5.12 ± 0.548
3.288AlaGln: 3.288 ± 0.507
7.437AlaArg: 7.437 ± 0.575
5.066AlaSer: 5.066 ± 0.665
6.467AlaThr: 6.467 ± 0.495
6.791AlaVal: 6.791 ± 0.583
2.425AlaTrp: 2.425 ± 0.367
2.264AlaTyr: 2.264 ± 0.359
0.0AlaXaa: 0.0 ± 0.0
Cys
1.024CysAla: 1.024 ± 0.359
0.162CysCys: 0.162 ± 0.088
1.186CysAsp: 1.186 ± 0.309
0.862CysGlu: 0.862 ± 0.239
0.269CysPhe: 0.269 ± 0.124
1.617CysGly: 1.617 ± 0.316
0.323CysHis: 0.323 ± 0.147
0.162CysIle: 0.162 ± 0.091
0.701CysLys: 0.701 ± 0.205
0.593CysLeu: 0.593 ± 0.194
0.216CysMet: 0.216 ± 0.117
0.431CysAsn: 0.431 ± 0.164
1.24CysPro: 1.24 ± 0.311
0.323CysGln: 0.323 ± 0.146
0.701CysArg: 0.701 ± 0.191
0.593CysSer: 0.593 ± 0.215
0.755CysThr: 0.755 ± 0.206
0.755CysVal: 0.755 ± 0.19
0.216CysTrp: 0.216 ± 0.1
0.162CysTyr: 0.162 ± 0.108
0.0CysXaa: 0.0 ± 0.0
Asp
7.006AspAla: 7.006 ± 0.601
0.862AspCys: 0.862 ± 0.195
4.689AspAsp: 4.689 ± 0.466
3.719AspGlu: 3.719 ± 0.469
1.94AspPhe: 1.94 ± 0.304
6.467AspGly: 6.467 ± 0.59
1.617AspHis: 1.617 ± 0.295
2.425AspIle: 2.425 ± 0.329
1.617AspLys: 1.617 ± 0.294
5.659AspLeu: 5.659 ± 0.523
1.024AspMet: 1.024 ± 0.237
1.725AspAsn: 1.725 ± 0.336
4.743AspPro: 4.743 ± 0.564
2.102AspGln: 2.102 ± 0.3
5.228AspArg: 5.228 ± 0.569
3.449AspSer: 3.449 ± 0.457
4.365AspThr: 4.365 ± 0.486
4.419AspVal: 4.419 ± 0.522
1.509AspTrp: 1.509 ± 0.3
2.048AspTyr: 2.048 ± 0.326
0.0AspXaa: 0.0 ± 0.0
Glu
5.874GluAla: 5.874 ± 0.525
0.97GluCys: 0.97 ± 0.247
2.856GluAsp: 2.856 ± 0.366
2.479GluGlu: 2.479 ± 0.46
2.695GluPhe: 2.695 ± 0.379
3.126GluGly: 3.126 ± 0.391
1.186GluHis: 1.186 ± 0.303
2.102GluIle: 2.102 ± 0.336
1.994GluLys: 1.994 ± 0.315
5.497GluLeu: 5.497 ± 0.503
1.455GluMet: 1.455 ± 0.302
2.156GluAsn: 2.156 ± 0.371
2.91GluPro: 2.91 ± 0.483
3.449GluGln: 3.449 ± 0.422
5.389GluArg: 5.389 ± 0.607
2.856GluSer: 2.856 ± 0.473
4.204GluThr: 4.204 ± 0.585
4.204GluVal: 4.204 ± 0.415
1.293GluTrp: 1.293 ± 0.266
1.886GluTyr: 1.886 ± 0.388
0.0GluXaa: 0.0 ± 0.0
Phe
3.126PheAla: 3.126 ± 0.449
0.269PheCys: 0.269 ± 0.141
2.695PheAsp: 2.695 ± 0.444
1.725PheGlu: 1.725 ± 0.329
0.862PhePhe: 0.862 ± 0.304
3.018PheGly: 3.018 ± 0.624
0.539PheHis: 0.539 ± 0.198
1.401PheIle: 1.401 ± 0.316
0.808PheLys: 0.808 ± 0.244
1.832PheLeu: 1.832 ± 0.279
0.755PheMet: 0.755 ± 0.2
1.455PheAsn: 1.455 ± 0.301
1.671PhePro: 1.671 ± 0.357
1.024PheGln: 1.024 ± 0.345
1.671PheArg: 1.671 ± 0.334
1.617PheSer: 1.617 ± 0.25
2.641PheThr: 2.641 ± 0.379
2.21PheVal: 2.21 ± 0.314
0.701PheTrp: 0.701 ± 0.199
0.916PheTyr: 0.916 ± 0.282
0.0PheXaa: 0.0 ± 0.0
Gly
8.785GlyAla: 8.785 ± 1.121
0.916GlyCys: 0.916 ± 0.224
6.521GlyAsp: 6.521 ± 0.63
4.419GlyGlu: 4.419 ± 0.517
2.587GlyPhe: 2.587 ± 0.391
10.887GlyGly: 10.887 ± 1.883
1.832GlyHis: 1.832 ± 0.303
4.042GlyIle: 4.042 ± 0.559
2.802GlyLys: 2.802 ± 0.407
5.928GlyLeu: 5.928 ± 0.504
2.533GlyMet: 2.533 ± 0.444
3.126GlyAsn: 3.126 ± 0.411
4.473GlyPro: 4.473 ± 0.579
2.264GlyGln: 2.264 ± 0.542
5.174GlyArg: 5.174 ± 0.752
5.982GlySer: 5.982 ± 0.996
6.521GlyThr: 6.521 ± 0.69
5.443GlyVal: 5.443 ± 0.462
2.533GlyTrp: 2.533 ± 0.401
2.21GlyTyr: 2.21 ± 0.374
0.0GlyXaa: 0.0 ± 0.0
His
1.94HisAla: 1.94 ± 0.363
0.377HisCys: 0.377 ± 0.162
0.97HisAsp: 0.97 ± 0.218
1.401HisGlu: 1.401 ± 0.341
0.485HisPhe: 0.485 ± 0.154
2.102HisGly: 2.102 ± 0.305
0.862HisHis: 0.862 ± 0.244
1.563HisIle: 1.563 ± 0.322
0.862HisLys: 0.862 ± 0.234
1.347HisLeu: 1.347 ± 0.267
0.593HisMet: 0.593 ± 0.163
0.97HisAsn: 0.97 ± 0.201
1.455HisPro: 1.455 ± 0.254
0.97HisGln: 0.97 ± 0.224
2.156HisArg: 2.156 ± 0.396
0.916HisSer: 0.916 ± 0.203
1.778HisThr: 1.778 ± 0.357
1.617HisVal: 1.617 ± 0.336
0.431HisTrp: 0.431 ± 0.151
1.078HisTyr: 1.078 ± 0.18
0.0HisXaa: 0.0 ± 0.0
Ile
5.066IleAla: 5.066 ± 0.535
0.593IleCys: 0.593 ± 0.206
3.611IleAsp: 3.611 ± 0.437
3.665IleGlu: 3.665 ± 0.365
0.808IlePhe: 0.808 ± 0.265
4.258IleGly: 4.258 ± 0.384
1.563IleHis: 1.563 ± 0.28
1.347IleIle: 1.347 ± 0.247
1.024IleLys: 1.024 ± 0.243
2.048IleLeu: 2.048 ± 0.421
0.323IleMet: 0.323 ± 0.13
1.886IleAsn: 1.886 ± 0.274
2.91IlePro: 2.91 ± 0.309
1.347IleGln: 1.347 ± 0.269
2.964IleArg: 2.964 ± 0.449
1.778IleSer: 1.778 ± 0.414
3.503IleThr: 3.503 ± 0.432
3.126IleVal: 3.126 ± 0.378
0.916IleTrp: 0.916 ± 0.202
0.647IleTyr: 0.647 ± 0.195
0.0IleXaa: 0.0 ± 0.0
Lys
3.665LysAla: 3.665 ± 0.43
0.431LysCys: 0.431 ± 0.138
1.994LysAsp: 1.994 ± 0.355
1.347LysGlu: 1.347 ± 0.223
1.455LysPhe: 1.455 ± 0.21
2.533LysGly: 2.533 ± 0.36
1.401LysHis: 1.401 ± 0.285
1.132LysIle: 1.132 ± 0.306
1.347LysLys: 1.347 ± 0.314
2.856LysLeu: 2.856 ± 0.439
0.647LysMet: 0.647 ± 0.171
0.862LysAsn: 0.862 ± 0.259
2.264LysPro: 2.264 ± 0.362
1.455LysGln: 1.455 ± 0.243
2.264LysArg: 2.264 ± 0.349
1.832LysSer: 1.832 ± 0.283
2.21LysThr: 2.21 ± 0.358
2.21LysVal: 2.21 ± 0.401
0.755LysTrp: 0.755 ± 0.264
1.132LysTyr: 1.132 ± 0.246
0.0LysXaa: 0.0 ± 0.0
Leu
7.599LeuAla: 7.599 ± 0.737
0.593LeuCys: 0.593 ± 0.217
5.066LeuAsp: 5.066 ± 0.566
3.773LeuGlu: 3.773 ± 0.415
2.802LeuPhe: 2.802 ± 0.296
5.497LeuGly: 5.497 ± 0.574
0.862LeuHis: 0.862 ± 0.209
3.826LeuIle: 3.826 ± 0.499
1.778LeuLys: 1.778 ± 0.3
4.797LeuLeu: 4.797 ± 0.565
1.563LeuMet: 1.563 ± 0.316
2.587LeuAsn: 2.587 ± 0.341
5.497LeuPro: 5.497 ± 0.707
2.91LeuGln: 2.91 ± 0.368
5.066LeuArg: 5.066 ± 0.637
5.659LeuSer: 5.659 ± 0.525
6.09LeuThr: 6.09 ± 0.476
4.743LeuVal: 4.743 ± 0.597
1.293LeuTrp: 1.293 ± 0.215
2.048LeuTyr: 2.048 ± 0.365
0.0LeuXaa: 0.0 ± 0.0
Met
2.371MetAla: 2.371 ± 0.33
0.054MetCys: 0.054 ± 0.059
1.186MetAsp: 1.186 ± 0.296
1.078MetGlu: 1.078 ± 0.196
0.647MetPhe: 0.647 ± 0.191
1.671MetGly: 1.671 ± 0.264
0.216MetHis: 0.216 ± 0.092
0.916MetIle: 0.916 ± 0.248
1.024MetLys: 1.024 ± 0.258
1.886MetLeu: 1.886 ± 0.262
0.647MetMet: 0.647 ± 0.239
0.916MetAsn: 0.916 ± 0.217
1.132MetPro: 1.132 ± 0.253
0.377MetGln: 0.377 ± 0.121
1.347MetArg: 1.347 ± 0.282
2.533MetSer: 2.533 ± 0.351
1.94MetThr: 1.94 ± 0.276
1.24MetVal: 1.24 ± 0.326
0.216MetTrp: 0.216 ± 0.106
0.377MetTyr: 0.377 ± 0.143
0.0MetXaa: 0.0 ± 0.0
Asn
3.557AsnAla: 3.557 ± 0.395
0.162AsnCys: 0.162 ± 0.08
1.886AsnAsp: 1.886 ± 0.332
1.725AsnGlu: 1.725 ± 0.327
0.862AsnPhe: 0.862 ± 0.305
3.88AsnGly: 3.88 ± 0.639
1.078AsnHis: 1.078 ± 0.234
1.563AsnIle: 1.563 ± 0.377
1.293AsnLys: 1.293 ± 0.252
2.749AsnLeu: 2.749 ± 0.418
0.539AsnMet: 0.539 ± 0.147
1.886AsnAsn: 1.886 ± 0.38
2.317AsnPro: 2.317 ± 0.3
1.078AsnGln: 1.078 ± 0.302
2.156AsnArg: 2.156 ± 0.373
1.455AsnSer: 1.455 ± 0.282
2.371AsnThr: 2.371 ± 0.345
1.886AsnVal: 1.886 ± 0.333
0.647AsnTrp: 0.647 ± 0.151
0.862AsnTyr: 0.862 ± 0.194
0.0AsnXaa: 0.0 ± 0.0
Pro
5.443ProAla: 5.443 ± 0.655
0.647ProCys: 0.647 ± 0.201
4.15ProAsp: 4.15 ± 0.448
4.312ProGlu: 4.312 ± 0.497
1.886ProPhe: 1.886 ± 0.311
6.036ProGly: 6.036 ± 0.572
1.671ProHis: 1.671 ± 0.289
2.048ProIle: 2.048 ± 0.315
2.102ProLys: 2.102 ± 0.37
4.581ProLeu: 4.581 ± 0.519
1.455ProMet: 1.455 ± 0.283
2.048ProAsn: 2.048 ± 0.345
4.419ProPro: 4.419 ± 0.608
2.21ProGln: 2.21 ± 0.333
3.341ProArg: 3.341 ± 0.566
3.018ProSer: 3.018 ± 0.411
3.018ProThr: 3.018 ± 0.436
5.174ProVal: 5.174 ± 0.536
1.132ProTrp: 1.132 ± 0.296
1.347ProTyr: 1.347 ± 0.236
0.0ProXaa: 0.0 ± 0.0
Gln
4.635GlnAla: 4.635 ± 0.573
0.539GlnCys: 0.539 ± 0.189
1.563GlnAsp: 1.563 ± 0.249
1.617GlnGlu: 1.617 ± 0.338
1.186GlnPhe: 1.186 ± 0.226
2.479GlnGly: 2.479 ± 0.546
0.916GlnHis: 0.916 ± 0.212
1.832GlnIle: 1.832 ± 0.308
1.24GlnLys: 1.24 ± 0.222
3.072GlnLeu: 3.072 ± 0.44
0.539GlnMet: 0.539 ± 0.184
0.808GlnAsn: 0.808 ± 0.253
2.317GlnPro: 2.317 ± 0.353
1.293GlnGln: 1.293 ± 0.32
2.587GlnArg: 2.587 ± 0.395
2.102GlnSer: 2.102 ± 0.338
1.725GlnThr: 1.725 ± 0.317
2.317GlnVal: 2.317 ± 0.344
0.647GlnTrp: 0.647 ± 0.173
0.808GlnTyr: 0.808 ± 0.269
0.0GlnXaa: 0.0 ± 0.0
Arg
6.629ArgAla: 6.629 ± 0.674
1.24ArgCys: 1.24 ± 0.316
4.365ArgAsp: 4.365 ± 0.53
5.012ArgGlu: 5.012 ± 0.629
2.21ArgPhe: 2.21 ± 0.346
4.312ArgGly: 4.312 ± 0.486
1.563ArgHis: 1.563 ± 0.317
3.988ArgIle: 3.988 ± 0.528
2.102ArgLys: 2.102 ± 0.352
5.443ArgLeu: 5.443 ± 0.598
2.371ArgMet: 2.371 ± 0.438
2.264ArgAsn: 2.264 ± 0.32
3.503ArgPro: 3.503 ± 0.45
1.94ArgGln: 1.94 ± 0.302
5.874ArgArg: 5.874 ± 0.747
3.719ArgSer: 3.719 ± 0.429
3.449ArgThr: 3.449 ± 0.452
5.228ArgVal: 5.228 ± 0.571
2.048ArgTrp: 2.048 ± 0.335
2.102ArgTyr: 2.102 ± 0.338
0.0ArgXaa: 0.0 ± 0.0
Ser
5.767SerAla: 5.767 ± 0.845
0.539SerCys: 0.539 ± 0.207
3.503SerAsp: 3.503 ± 0.406
2.91SerGlu: 2.91 ± 0.414
2.048SerPhe: 2.048 ± 0.419
6.252SerGly: 6.252 ± 0.881
1.078SerHis: 1.078 ± 0.232
2.802SerIle: 2.802 ± 0.411
2.587SerLys: 2.587 ± 0.39
4.419SerLeu: 4.419 ± 0.471
1.293SerMet: 1.293 ± 0.23
2.425SerAsn: 2.425 ± 0.344
3.234SerPro: 3.234 ± 0.337
1.563SerGln: 1.563 ± 0.296
3.126SerArg: 3.126 ± 0.438
3.503SerSer: 3.503 ± 0.619
3.072SerThr: 3.072 ± 0.411
4.258SerVal: 4.258 ± 0.469
1.617SerTrp: 1.617 ± 0.259
1.509SerTyr: 1.509 ± 0.229
0.0SerXaa: 0.0 ± 0.0
Thr
5.982ThrAla: 5.982 ± 0.616
0.808ThrCys: 0.808 ± 0.218
4.204ThrAsp: 4.204 ± 0.6
3.826ThrGlu: 3.826 ± 0.42
2.048ThrPhe: 2.048 ± 0.317
6.252ThrGly: 6.252 ± 0.544
1.94ThrHis: 1.94 ± 0.306
3.341ThrIle: 3.341 ± 0.465
2.156ThrLys: 2.156 ± 0.327
4.689ThrLeu: 4.689 ± 0.47
1.347ThrMet: 1.347 ± 0.273
2.425ThrAsn: 2.425 ± 0.31
4.635ThrPro: 4.635 ± 0.494
2.102ThrGln: 2.102 ± 0.373
4.258ThrArg: 4.258 ± 0.414
3.719ThrSer: 3.719 ± 0.355
5.066ThrThr: 5.066 ± 0.671
6.144ThrVal: 6.144 ± 0.68
1.024ThrTrp: 1.024 ± 0.24
2.102ThrTyr: 2.102 ± 0.312
0.0ThrXaa: 0.0 ± 0.0
Val
6.683ValAla: 6.683 ± 0.534
1.509ValCys: 1.509 ± 0.279
5.497ValAsp: 5.497 ± 0.502
4.797ValGlu: 4.797 ± 0.521
1.994ValPhe: 1.994 ± 0.341
5.713ValGly: 5.713 ± 0.637
1.563ValHis: 1.563 ± 0.338
2.641ValIle: 2.641 ± 0.395
2.587ValLys: 2.587 ± 0.356
4.85ValLeu: 4.85 ± 0.564
1.347ValMet: 1.347 ± 0.242
2.317ValAsn: 2.317 ± 0.318
3.988ValPro: 3.988 ± 0.422
2.695ValGln: 2.695 ± 0.317
4.419ValArg: 4.419 ± 0.61
5.228ValSer: 5.228 ± 0.577
5.389ValThr: 5.389 ± 0.532
5.821ValVal: 5.821 ± 0.805
1.563ValTrp: 1.563 ± 0.293
1.347ValTyr: 1.347 ± 0.218
0.0ValXaa: 0.0 ± 0.0
Trp
2.156TrpAla: 2.156 ± 0.285
0.323TrpCys: 0.323 ± 0.111
1.455TrpAsp: 1.455 ± 0.284
1.024TrpGlu: 1.024 ± 0.298
0.755TrpPhe: 0.755 ± 0.202
0.862TrpGly: 0.862 ± 0.222
0.701TrpHis: 0.701 ± 0.22
0.916TrpIle: 0.916 ± 0.221
0.916TrpLys: 0.916 ± 0.194
1.832TrpLeu: 1.832 ± 0.327
0.755TrpMet: 0.755 ± 0.224
0.485TrpAsn: 0.485 ± 0.153
0.916TrpPro: 0.916 ± 0.23
1.024TrpGln: 1.024 ± 0.233
2.21TrpArg: 2.21 ± 0.4
1.347TrpSer: 1.347 ± 0.278
1.617TrpThr: 1.617 ± 0.301
1.832TrpVal: 1.832 ± 0.412
0.97TrpTrp: 0.97 ± 0.194
0.323TrpTyr: 0.323 ± 0.134
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.371TyrAla: 2.371 ± 0.362
0.323TyrCys: 0.323 ± 0.124
1.886TyrAsp: 1.886 ± 0.365
1.671TyrGlu: 1.671 ± 0.251
0.755TyrPhe: 0.755 ± 0.202
2.264TyrGly: 2.264 ± 0.398
0.431TyrHis: 0.431 ± 0.163
1.24TyrIle: 1.24 ± 0.209
0.862TyrLys: 0.862 ± 0.194
2.264TyrLeu: 2.264 ± 0.297
0.108TyrMet: 0.108 ± 0.074
0.808TyrAsn: 0.808 ± 0.21
1.293TyrPro: 1.293 ± 0.216
0.862TyrGln: 0.862 ± 0.24
1.994TyrArg: 1.994 ± 0.336
1.132TyrSer: 1.132 ± 0.22
1.886TyrThr: 1.886 ± 0.296
2.533TyrVal: 2.533 ± 0.335
0.485TyrTrp: 0.485 ± 0.155
0.647TyrTyr: 0.647 ± 0.171
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 109 proteins (18556 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski