Brevibacillus fluminis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Brevibacillus

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5987 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3M8DSU6|A0A3M8DSU6_9BACL Uncharacterized protein OS=Brevibacillus fluminis OX=511487 GN=EDM56_06640 PE=4 SV=1
MM1 pKa = 7.47PAVPTFVTFFPDD13 pKa = 2.95EE14 pKa = 3.74RR15 pKa = 11.84AAQHH19 pKa = 6.07FVSQLALDD27 pKa = 4.01TSISSITMLNEE38 pKa = 3.74SAGTQDD44 pKa = 4.29PFDD47 pKa = 3.84EE48 pKa = 4.66TEE50 pKa = 4.14SYY52 pKa = 11.58ANSLQQHH59 pKa = 5.94GFSLEE64 pKa = 3.88QCSNCEE70 pKa = 4.11SKK72 pKa = 10.49IGEE75 pKa = 4.25GNIALLIEE83 pKa = 4.97GNDD86 pKa = 3.41ATDD89 pKa = 3.4ILLALQEE96 pKa = 4.44YY97 pKa = 9.88GVQSYY102 pKa = 11.32HH103 pKa = 5.53MVV105 pKa = 3.03

Molecular weight: 11.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3M8DR85|A0A3M8DR85_9BACL TIGR01777 family protein OS=Brevibacillus fluminis OX=511487 GN=EDM56_09190 PE=3 SV=1
MM1 pKa = 7.47KK2 pKa = 9.96MSRR5 pKa = 11.84RR6 pKa = 11.84NLLSVLITSGIVAAIGYY23 pKa = 8.41MLMPKK28 pKa = 9.77RR29 pKa = 11.84RR30 pKa = 11.84NGFSLNINRR39 pKa = 11.84LPLSRR44 pKa = 11.84KK45 pKa = 9.72NMMKK49 pKa = 10.34AGAMLFRR56 pKa = 11.84SLRR59 pKa = 11.84TAVARR64 pKa = 4.15

Molecular weight: 7.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5987

0

5987

1876288

25

2086

313.4

34.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.65 ± 0.037

0.814 ± 0.01

4.897 ± 0.026

6.71 ± 0.032

4.181 ± 0.024

7.348 ± 0.032

2.171 ± 0.015

6.766 ± 0.03

5.479 ± 0.03

10.167 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.84 ± 0.016

3.513 ± 0.02

4.095 ± 0.022

4.166 ± 0.026

4.907 ± 0.027

5.879 ± 0.027

5.659 ± 0.03

7.442 ± 0.023

1.141 ± 0.011

3.175 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski