Nocardioides sp. J9

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Propionibacteriales; Nocardioidaceae; Nocardioides; unclassified Nocardioides

Average proteome isoelectric point is 5.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4501 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A562D393|A0A562D393_9ACTN Uncharacterized protein OS=Nocardioides sp. J9 OX=935844 GN=L615_010600000040 PE=4 SV=1
MM1 pKa = 7.51RR2 pKa = 11.84AMRR5 pKa = 11.84SRR7 pKa = 11.84LLAGVPALLLLASLSACNDD26 pKa = 3.79DD27 pKa = 5.69DD28 pKa = 5.56PEE30 pKa = 6.15ASDD33 pKa = 3.87DD34 pKa = 3.86TGTSSEE40 pKa = 4.42GGTSCEE46 pKa = 4.0YY47 pKa = 11.21VEE49 pKa = 4.49TGGAARR55 pKa = 11.84DD56 pKa = 3.9VEE58 pKa = 4.79LPPATTDD65 pKa = 3.69LSGEE69 pKa = 4.35VPAVLHH75 pKa = 5.83TSIGDD80 pKa = 3.6LKK82 pKa = 10.19ITLDD86 pKa = 3.84AEE88 pKa = 4.32KK89 pKa = 10.55APCTVASFASLAEE102 pKa = 3.74QGYY105 pKa = 10.53YY106 pKa = 10.72DD107 pKa = 3.49GTSCHH112 pKa = 6.21RR113 pKa = 11.84QGNDD117 pKa = 2.87PGFEE121 pKa = 4.1FLQCGDD127 pKa = 3.78PFYY130 pKa = 11.23DD131 pKa = 4.89PEE133 pKa = 6.37AEE135 pKa = 4.22QQDD138 pKa = 4.13PAVGTGNPGYY148 pKa = 10.18NIPDD152 pKa = 4.32EE153 pKa = 4.43IDD155 pKa = 3.23GTEE158 pKa = 4.0TYY160 pKa = 9.97EE161 pKa = 4.25AGTIAMANTGAPNSGGGQFFIVFGDD186 pKa = 3.77SQFPPSYY193 pKa = 9.68TVWAHH198 pKa = 6.51LDD200 pKa = 3.4AEE202 pKa = 4.32AVEE205 pKa = 4.48LVKK208 pKa = 10.75KK209 pKa = 10.39AAASGNDD216 pKa = 3.36GSSRR220 pKa = 11.84AGGGVPNTPVTFEE233 pKa = 4.84GIDD236 pKa = 3.26VGG238 pKa = 4.78

Molecular weight: 24.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A562C1B4|A0A562C1B4_9ACTN Methylase involved in ubiquinone/menaquinone biosynthesis OS=Nocardioides sp. J9 OX=935844 GN=L615_008200000200 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.99KK16 pKa = 9.77HH17 pKa = 5.81RR18 pKa = 11.84KK19 pKa = 8.51LLKK22 pKa = 8.15KK23 pKa = 9.24TRR25 pKa = 11.84VQRR28 pKa = 11.84RR29 pKa = 11.84KK30 pKa = 10.07LGKK33 pKa = 9.87

Molecular weight: 4.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4501

0

4501

1420892

23

2580

315.7

33.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.149 ± 0.054

0.731 ± 0.011

6.594 ± 0.028

5.986 ± 0.038

2.727 ± 0.023

9.271 ± 0.037

2.16 ± 0.021

3.354 ± 0.03

2.064 ± 0.03

10.095 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.775 ± 0.016

1.784 ± 0.022

5.752 ± 0.032

2.769 ± 0.02

7.685 ± 0.048

5.001 ± 0.025

6.048 ± 0.035

9.606 ± 0.035

1.504 ± 0.016

1.906 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski