Papaya cytorhabdovirus
Average proteome isoelectric point is 6.8
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A386GWS9|A0A386GWS9_9RHAB Large structural protein OS=Papaya cytorhabdovirus OX=2364291 GN=L PE=4 SV=1
MM1 pKa = 7.77 PKK3 pKa = 9.59 TYY5 pKa = 10.44 KK6 pKa = 10.82 DD7 pKa = 3.13 NSAYY11 pKa = 10.27 AKK13 pKa = 10.34 EE14 pKa = 4.16 IFTPITKK21 pKa = 9.95 EE22 pKa = 3.52 ISHH25 pKa = 6.35 GRR27 pKa = 11.84 IQKK30 pKa = 7.46 QTWTDD35 pKa = 3.36 QVFEE39 pKa = 4.15 EE40 pKa = 4.18 MQVYY44 pKa = 10.2 KK45 pKa = 10.49 INKK48 pKa = 6.49 YY49 pKa = 7.1 TAGDD53 pKa = 3.64 LEE55 pKa = 4.72 RR56 pKa = 11.84 YY57 pKa = 8.46 GMYY60 pKa = 9.77 MVDD63 pKa = 4.25 SISKK67 pKa = 10.2 KK68 pKa = 8.57 EE69 pKa = 3.9 VSMDD73 pKa = 2.84 TCYY76 pKa = 10.96 AILILACHH84 pKa = 6.24 LHH86 pKa = 6.7 KK87 pKa = 10.44 IAPSKK92 pKa = 11.08 DD93 pKa = 3.1 FTLFEE98 pKa = 4.84 LPLKK102 pKa = 9.79 GTLPLPKK109 pKa = 9.34 MEE111 pKa = 6.05 VFAAWNKK118 pKa = 8.69 EE119 pKa = 3.64 QAEE122 pKa = 4.32 EE123 pKa = 4.45 GGEE126 pKa = 3.94 EE127 pKa = 4.01 DD128 pKa = 3.83 GKK130 pKa = 11.31 GSEE133 pKa = 4.22 EE134 pKa = 4.44 SEE136 pKa = 4.02 DD137 pKa = 3.78 DD138 pKa = 3.98 SEE140 pKa = 5.01 HH141 pKa = 6.71 EE142 pKa = 4.78 EE143 pKa = 4.34 ISVDD147 pKa = 3.35 PSKK150 pKa = 11.15 GGKK153 pKa = 9.15 EE154 pKa = 3.88 KK155 pKa = 10.57 TEE157 pKa = 4.51 DD158 pKa = 3.8 PQSATAIDD166 pKa = 3.76 KK167 pKa = 10.31 EE168 pKa = 4.19 RR169 pKa = 11.84 AANCVRR175 pKa = 11.84 FFCFCAAFYY184 pKa = 11.06 LRR186 pKa = 11.84 LCIKK190 pKa = 10.21 DD191 pKa = 3.43 IQDD194 pKa = 3.23 LSTPYY199 pKa = 10.16 TRR201 pKa = 11.84 MKK203 pKa = 10.18 EE204 pKa = 3.85 RR205 pKa = 11.84 YY206 pKa = 6.74 TRR208 pKa = 11.84 FYY210 pKa = 10.68 QGVIPTSGEE219 pKa = 4.21 DD220 pKa = 3.38 IPPSEE225 pKa = 4.48 SVKK228 pKa = 10.54 SLKK231 pKa = 9.89 TFFAGDD237 pKa = 4.26 RR238 pKa = 11.84 IISRR242 pKa = 11.84 TWVKK246 pKa = 10.66 LFVPWHH252 pKa = 6.91 DD253 pKa = 3.57 KK254 pKa = 11.71 AEE256 pKa = 4.25 VGSPQDD262 pKa = 3.09 GMVIYY267 pKa = 10.41 LAVLPFAFSGMHH279 pKa = 6.44 ALKK282 pKa = 11.03 LMDD285 pKa = 5.13 DD286 pKa = 4.56 VIEE289 pKa = 4.17 MTHH292 pKa = 6.94 LKK294 pKa = 9.39 DD295 pKa = 3.08 TDD297 pKa = 4.94 LIYY300 pKa = 11.16 QMMHH304 pKa = 6.54 PRR306 pKa = 11.84 HH307 pKa = 5.89 NSALASISEE316 pKa = 4.77 IYY318 pKa = 9.88 AKK320 pKa = 10.17 HH321 pKa = 5.49 YY322 pKa = 10.2 PEE324 pKa = 5.43 EE325 pKa = 4.86 GSQKK329 pKa = 8.74 NTYY332 pKa = 8.66 VKK334 pKa = 9.43 YY335 pKa = 10.57 ARR337 pKa = 11.84 VVGPQFFPALQTKK350 pKa = 8.68 KK351 pKa = 10.68 CKK353 pKa = 10.58 GLVCVLANLVVLHH366 pKa = 5.96 QKK368 pKa = 6.68 TTEE371 pKa = 3.93 EE372 pKa = 4.09 TGDD375 pKa = 3.54 PRR377 pKa = 11.84 SIAGIDD383 pKa = 4.15 DD384 pKa = 3.74 ISDD387 pKa = 3.33 EE388 pKa = 4.09 LMKK391 pKa = 10.63 FGKK394 pKa = 10.31 RR395 pKa = 11.84 MAKK398 pKa = 9.95 IMMKK402 pKa = 10.06 NAPKK406 pKa = 8.91 TASYY410 pKa = 9.27 MYY412 pKa = 10.8 SSTAKK417 pKa = 9.83 EE418 pKa = 3.98 AEE420 pKa = 4.22 EE421 pKa = 5.29 GGDD424 pKa = 3.65 STDD427 pKa = 3.66 EE428 pKa = 3.88 EE429 pKa = 4.78 SEE431 pKa = 4.04 EE432 pKa = 4.26 DD433 pKa = 4.22 EE434 pKa = 4.68 EE435 pKa = 7.05 DD436 pKa = 4.13 IDD438 pKa = 5.02 PEE440 pKa = 4.14 LLKK443 pKa = 10.83 VGKK446 pKa = 10.08 RR447 pKa = 11.84 IYY449 pKa = 10.62 KK450 pKa = 9.47 PP451 pKa = 3.2
Molecular weight: 51.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.154
IPC2_protein 5.207
IPC_protein 5.169
Toseland 5.169
ProMoST 5.334
Dawson 5.181
Bjellqvist 5.308
Wikipedia 5.054
Rodwell 5.118
Grimsley 5.105
Solomon 5.181
Lehninger 5.143
Nozaki 5.321
DTASelect 5.461
Thurlkill 5.181
EMBOSS 5.13
Sillero 5.397
Patrickios 4.05
IPC_peptide 5.194
IPC2_peptide 5.397
IPC2.peptide.svr19 5.367
Protein with the highest isoelectric point:
>tr|A0A386GV82|A0A386GV82_9RHAB Nucleocapsid protein OS=Papaya cytorhabdovirus OX=2364291 GN=N PE=4 SV=1
MM1 pKa = 7.54 EE2 pKa = 5.76 KK3 pKa = 10.58 SNSAKK8 pKa = 10.58 GPISSLVSAHH18 pKa = 5.46 VSSSKK23 pKa = 10.21 RR24 pKa = 11.84 IKK26 pKa = 10.95 VKK28 pKa = 10.14 VQQHH32 pKa = 4.1 QEE34 pKa = 4.0 EE35 pKa = 4.7 YY36 pKa = 10.6 SIRR39 pKa = 11.84 LPFSTKK45 pKa = 10.47 KK46 pKa = 9.55 LTSEE50 pKa = 4.32 YY51 pKa = 10.47 ATVTLLEE58 pKa = 4.29 IFYY61 pKa = 10.55 KK62 pKa = 10.88 GYY64 pKa = 10.31 IFNAQGSTIEE74 pKa = 3.87 VVIRR78 pKa = 11.84 DD79 pKa = 3.96 KK80 pKa = 11.31 RR81 pKa = 11.84 SVSQTEE87 pKa = 3.95 QKK89 pKa = 10.17 RR90 pKa = 11.84 LAVIIPASKK99 pKa = 10.1 SCYY102 pKa = 8.49 LTVKK106 pKa = 9.82 GFSSAPIEE114 pKa = 4.19 EE115 pKa = 4.61 GCPYY119 pKa = 9.92 EE120 pKa = 4.89 VSIKK124 pKa = 9.82 TVVRR128 pKa = 11.84 NIRR131 pKa = 11.84 FGITLGRR138 pKa = 11.84 LDD140 pKa = 3.7 IRR142 pKa = 11.84 PHH144 pKa = 6.27 LFYY147 pKa = 11.11 SNHH150 pKa = 5.94 FEE152 pKa = 4.68 DD153 pKa = 5.47 GDD155 pKa = 4.0 YY156 pKa = 10.72 LTVNKK161 pKa = 10.04 VEE163 pKa = 4.2 GPEE166 pKa = 4.08 LSLKK170 pKa = 10.58 RR171 pKa = 11.84 GSLTNKK177 pKa = 7.13 EE178 pKa = 3.94 APYY181 pKa = 9.34 TVSLIPTEE189 pKa = 4.02
Molecular weight: 21.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.776
IPC2_protein 8.756
IPC_protein 8.682
Toseland 9.326
ProMoST 9.121
Dawson 9.619
Bjellqvist 9.355
Wikipedia 9.794
Rodwell 9.867
Grimsley 9.692
Solomon 9.677
Lehninger 9.633
Nozaki 9.414
DTASelect 9.311
Thurlkill 9.458
EMBOSS 9.765
Sillero 9.56
Patrickios 4.8
IPC_peptide 9.663
IPC2_peptide 7.951
IPC2.peptide.svr19 7.752
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8
0
8
3985
26
2113
498.1
56.49
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.868 ± 0.557
1.807 ± 0.379
5.445 ± 0.527
7.202 ± 1.142
4.768 ± 0.7
5.32 ± 0.186
1.907 ± 0.18
6.349 ± 0.22
7.955 ± 1.183
9.26 ± 0.879
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.061 ± 0.297
3.614 ± 0.4
4.567 ± 0.258
2.886 ± 0.321
4.592 ± 0.463
9.184 ± 0.55
5.621 ± 0.244
6.851 ± 0.394
1.305 ± 0.29
3.438 ± 0.282
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here