Amino acid dipepetide frequency for Clostridium sp. CAG:75

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.834AlaAla: 6.834 ± 0.142	1.025AlaCys: 1.025 ± 0.04	4.61AlaAsp: 4.61 ± 0.081	5.164AlaGlu: 5.164 ± 0.102	2.836AlaPhe: 2.836 ± 0.065	5.694AlaGly: 5.694 ± 0.111	1.171AlaHis: 1.171 ± 0.043	4.914AlaIle: 4.914 ± 0.09	5.991AlaLys: 5.991 ± 0.106	6.678AlaLeu: 6.678 ± 0.096	2.406AlaMet: 2.406 ± 0.067	2.895AlaAsn: 2.895 ± 0.07	1.98AlaPro: 1.98 ± 0.061	2.62AlaGln: 2.62 ± 0.064	2.699AlaArg: 2.699 ± 0.067	4.302AlaSer: 4.302 ± 0.095	3.665AlaThr: 3.665 ± 0.073	6.131AlaVal: 6.131 ± 0.111	0.605AlaTrp: 0.605 ± 0.027	2.804AlaTyr: 2.804 ± 0.065	0.025AlaXaa: 0.025 ± 0.006
Cys	0.981CysAla: 0.981 ± 0.038	0.297CysCys: 0.297 ± 0.021	0.895CysAsp: 0.895 ± 0.044	0.934CysGlu: 0.934 ± 0.041	0.694CysPhe: 0.694 ± 0.033	1.371CysGly: 1.371 ± 0.05	0.316CysHis: 0.316 ± 0.023	1.106CysIle: 1.106 ± 0.049	0.955CysLys: 0.955 ± 0.044	1.212CysLeu: 1.212 ± 0.045	0.466CysMet: 0.466 ± 0.026	0.579CysAsn: 0.579 ± 0.026	0.561CysPro: 0.561 ± 0.033	0.432CysGln: 0.432 ± 0.029	0.674CysArg: 0.674 ± 0.035	0.872CysSer: 0.872 ± 0.032	0.788CysThr: 0.788 ± 0.044	1.029CysVal: 1.029 ± 0.036	0.095CysTrp: 0.095 ± 0.012	0.662CysTyr: 0.662 ± 0.028	0.003CysXaa: 0.003 ± 0.002
Asp	4.544AspAla: 4.544 ± 0.088	0.843AspCys: 0.843 ± 0.037	3.247AspAsp: 3.247 ± 0.079	4.379AspGlu: 4.379 ± 0.084	2.354AspPhe: 2.354 ± 0.068	4.412AspGly: 4.412 ± 0.088	1.097AspHis: 1.097 ± 0.041	4.442AspIle: 4.442 ± 0.1	4.076AspLys: 4.076 ± 0.084	4.532AspLeu: 4.532 ± 0.09	1.891AspMet: 1.891 ± 0.044	2.24AspAsn: 2.24 ± 0.055	1.898AspPro: 1.898 ± 0.052	1.83AspGln: 1.83 ± 0.053	2.546AspArg: 2.546 ± 0.066	3.089AspSer: 3.089 ± 0.074	3.211AspThr: 3.211 ± 0.072	4.083AspVal: 4.083 ± 0.083	0.553AspTrp: 0.553 ± 0.032	2.784AspTyr: 2.784 ± 0.065	0.004AspXaa: 0.004 ± 0.002
Glu	4.805GluAla: 4.805 ± 0.102	0.849GluCys: 0.849 ± 0.038	4.214GluAsp: 4.214 ± 0.094	6.593GluGlu: 6.593 ± 0.115	2.241GluPhe: 2.241 ± 0.067	3.945GluGly: 3.945 ± 0.083	1.502GluHis: 1.502 ± 0.055	5.259GluIle: 5.259 ± 0.105	5.457GluLys: 5.457 ± 0.092	6.086GluLeu: 6.086 ± 0.097	2.432GluMet: 2.432 ± 0.066	3.361GluAsn: 3.361 ± 0.068	1.751GluPro: 1.751 ± 0.053	3.966GluGln: 3.966 ± 0.095	3.37GluArg: 3.37 ± 0.081	3.325GluSer: 3.325 ± 0.068	3.626GluThr: 3.626 ± 0.071	4.417GluVal: 4.417 ± 0.099	0.648GluTrp: 0.648 ± 0.033	3.133GluTyr: 3.133 ± 0.066	0.006GluXaa: 0.006 ± 0.003
Phe	3.188PheAla: 3.188 ± 0.067	0.743PheCys: 0.743 ± 0.033	2.491PheAsp: 2.491 ± 0.061	2.429PheGlu: 2.429 ± 0.066	1.482PhePhe: 1.482 ± 0.052	2.852PheGly: 2.852 ± 0.063	0.813PheHis: 0.813 ± 0.038	2.49PheIle: 2.49 ± 0.067	1.706PheLys: 1.706 ± 0.05	3.473PheLeu: 3.473 ± 0.096	1.194PheMet: 1.194 ± 0.047	1.318PheAsn: 1.318 ± 0.04	1.235PhePro: 1.235 ± 0.045	1.348PheGln: 1.348 ± 0.044	1.823PheArg: 1.823 ± 0.049	2.478PheSer: 2.478 ± 0.051	2.14PheThr: 2.14 ± 0.056	2.94PheVal: 2.94 ± 0.073	0.361PheTrp: 0.361 ± 0.028	1.679PheTyr: 1.679 ± 0.058	0.001PheXaa: 0.001 ± 0.002
Gly	4.824GlyAla: 4.824 ± 0.09	1.15GlyCys: 1.15 ± 0.048	3.646GlyAsp: 3.646 ± 0.075	4.438GlyGlu: 4.438 ± 0.071	2.777GlyPhe: 2.777 ± 0.072	4.352GlyGly: 4.352 ± 0.112	1.274GlyHis: 1.274 ± 0.043	5.662GlyIle: 5.662 ± 0.098	5.757GlyLys: 5.757 ± 0.09	5.313GlyLeu: 5.313 ± 0.093	2.459GlyMet: 2.459 ± 0.067	3.054GlyAsn: 3.054 ± 0.082	1.146GlyPro: 1.146 ± 0.046	2.298GlyGln: 2.298 ± 0.061	2.978GlyArg: 2.978 ± 0.065	3.972GlySer: 3.972 ± 0.086	4.173GlyThr: 4.173 ± 0.083	4.945GlyVal: 4.945 ± 0.092	0.59GlyTrp: 0.59 ± 0.034	3.194GlyTyr: 3.194 ± 0.07	0.022GlyXaa: 0.022 ± 0.005
His	1.198HisAla: 1.198 ± 0.044	0.338HisCys: 0.338 ± 0.021	0.909HisAsp: 0.909 ± 0.04	1.019HisGlu: 1.019 ± 0.035	0.882HisPhe: 0.882 ± 0.034	1.427HisGly: 1.427 ± 0.049	0.465HisHis: 0.465 ± 0.029	1.52HisIle: 1.52 ± 0.043	1.207HisLys: 1.207 ± 0.042	1.592HisLeu: 1.592 ± 0.046	0.599HisMet: 0.599 ± 0.031	0.766HisAsn: 0.766 ± 0.033	0.863HisPro: 0.863 ± 0.045	0.671HisGln: 0.671 ± 0.033	0.892HisArg: 0.892 ± 0.035	1.072HisSer: 1.072 ± 0.039	1.069HisThr: 1.069 ± 0.042	1.24HisVal: 1.24 ± 0.043	0.204HisTrp: 0.204 ± 0.02	0.888HisTyr: 0.888 ± 0.037	0.0HisXaa: 0.0 ± 0.0
Ile	6.007IleAla: 6.007 ± 0.118	1.404IleCys: 1.404 ± 0.048	4.227IleAsp: 4.227 ± 0.075	4.378IleGlu: 4.378 ± 0.08	2.644IlePhe: 2.644 ± 0.066	5.104IleGly: 5.104 ± 0.093	1.383IleHis: 1.383 ± 0.041	4.747IleIle: 4.747 ± 0.097	4.069IleLys: 4.069 ± 0.096	6.462IleLeu: 6.462 ± 0.123	2.064IleMet: 2.064 ± 0.057	2.801IleAsn: 2.801 ± 0.064	3.064IlePro: 3.064 ± 0.069	2.407IleGln: 2.407 ± 0.057	3.686IleArg: 3.686 ± 0.075	4.673IleSer: 4.673 ± 0.093	4.516IleThr: 4.516 ± 0.088	5.275IleVal: 5.275 ± 0.097	0.608IleTrp: 0.608 ± 0.033	2.709IleTyr: 2.709 ± 0.066	0.009IleXaa: 0.009 ± 0.003
Lys	5.294LysAla: 5.294 ± 0.115	0.73LysCys: 0.73 ± 0.035	4.22LysAsp: 4.22 ± 0.08	6.122LysGlu: 6.122 ± 0.107	1.882LysPhe: 1.882 ± 0.057	4.174LysGly: 4.174 ± 0.089	1.205LysHis: 1.205 ± 0.043	4.991LysIle: 4.991 ± 0.096	7.02LysLys: 7.02 ± 0.154	5.812LysLeu: 5.812 ± 0.095	2.422LysMet: 2.422 ± 0.051	3.526LysAsn: 3.526 ± 0.082	1.986LysPro: 1.986 ± 0.058	3.256LysGln: 3.256 ± 0.084	3.272LysArg: 3.272 ± 0.065	3.467LysSer: 3.467 ± 0.078	3.908LysThr: 3.908 ± 0.1	4.737LysVal: 4.737 ± 0.096	0.688LysTrp: 0.688 ± 0.042	3.195LysTyr: 3.195 ± 0.067	0.001LysXaa: 0.001 ± 0.002
Leu	6.502LeuAla: 6.502 ± 0.111	1.554LeuCys: 1.554 ± 0.048	4.952LeuAsp: 4.952 ± 0.098	5.892LeuGlu: 5.892 ± 0.109	3.753LeuPhe: 3.753 ± 0.1	5.414LeuGly: 5.414 ± 0.099	1.759LeuHis: 1.759 ± 0.051	5.415LeuIle: 5.415 ± 0.107	5.29LeuLys: 5.29 ± 0.101	8.492LeuLeu: 8.492 ± 0.16	2.38LeuMet: 2.38 ± 0.056	3.198LeuAsn: 3.198 ± 0.07	3.18LeuPro: 3.18 ± 0.075	4.025LeuGln: 4.025 ± 0.084	4.033LeuArg: 4.033 ± 0.088	5.998LeuSer: 5.998 ± 0.116	5.023LeuThr: 5.023 ± 0.093	5.568LeuVal: 5.568 ± 0.095	0.712LeuTrp: 0.712 ± 0.035	3.359LeuTyr: 3.359 ± 0.074	0.007LeuXaa: 0.007 ± 0.003
Met	2.295MetAla: 2.295 ± 0.057	0.362MetCys: 0.362 ± 0.022	1.951MetAsp: 1.951 ± 0.055	2.256MetGlu: 2.256 ± 0.062	1.019MetPhe: 1.019 ± 0.041	1.948MetGly: 1.948 ± 0.053	0.582MetHis: 0.582 ± 0.029	2.433MetIle: 2.433 ± 0.067	2.457MetLys: 2.457 ± 0.06	2.814MetLeu: 2.814 ± 0.061	0.977MetMet: 0.977 ± 0.038	1.533MetAsn: 1.533 ± 0.048	1.111MetPro: 1.111 ± 0.039	1.373MetGln: 1.373 ± 0.044	1.462MetArg: 1.462 ± 0.046	1.847MetSer: 1.847 ± 0.056	1.934MetThr: 1.934 ± 0.05	1.995MetVal: 1.995 ± 0.054	0.191MetTrp: 0.191 ± 0.017	1.061MetTyr: 1.061 ± 0.045	0.003MetXaa: 0.003 ± 0.002
Asn	3.214AsnAla: 3.214 ± 0.071	0.673AsnCys: 0.673 ± 0.034	2.298AsnAsp: 2.298 ± 0.06	2.602AsnGlu: 2.602 ± 0.071	1.429AsnPhe: 1.429 ± 0.043	3.421AsnGly: 3.421 ± 0.077	0.853AsnHis: 0.853 ± 0.029	3.292AsnIle: 3.292 ± 0.073	2.751AsnLys: 2.751 ± 0.077	3.392AsnLeu: 3.392 ± 0.067	1.41AsnMet: 1.41 ± 0.04	1.898AsnAsn: 1.898 ± 0.066	1.716AsnPro: 1.716 ± 0.06	1.267AsnGln: 1.267 ± 0.043	2.016AsnArg: 2.016 ± 0.054	2.195AsnSer: 2.195 ± 0.069	2.536AsnThr: 2.536 ± 0.069	3.025AsnVal: 3.025 ± 0.078	0.348AsnTrp: 0.348 ± 0.022	1.839AsnTyr: 1.839 ± 0.063	0.003AsnXaa: 0.003 ± 0.002
Pro	2.237ProAla: 2.237 ± 0.063	0.419ProCys: 0.419 ± 0.028	2.074ProAsp: 2.074 ± 0.053	2.846ProGlu: 2.846 ± 0.068	1.404ProPhe: 1.404 ± 0.049	2.096ProGly: 2.096 ± 0.06	0.537ProHis: 0.537 ± 0.028	2.136ProIle: 2.136 ± 0.056	2.156ProLys: 2.156 ± 0.061	2.507ProLeu: 2.507 ± 0.061	0.928ProMet: 0.928 ± 0.037	1.293ProAsn: 1.293 ± 0.043	0.624ProPro: 0.624 ± 0.032	1.106ProGln: 1.106 ± 0.043	0.932ProArg: 0.932 ± 0.04	1.869ProSer: 1.869 ± 0.076	1.715ProThr: 1.715 ± 0.049	2.643ProVal: 2.643 ± 0.058	0.305ProTrp: 0.305 ± 0.023	1.377ProTyr: 1.377 ± 0.047	0.009ProXaa: 0.009 ± 0.004
Gln	2.749GlnAla: 2.749 ± 0.072	0.463GlnCys: 0.463 ± 0.029	1.921GlnAsp: 1.921 ± 0.061	3.027GlnGlu: 3.027 ± 0.078	1.384GlnPhe: 1.384 ± 0.044	2.142GlnGly: 2.142 ± 0.058	0.616GlnHis: 0.616 ± 0.032	3.169GlnIle: 3.169 ± 0.075	3.36GlnLys: 3.36 ± 0.079	3.165GlnLeu: 3.165 ± 0.073	1.534GlnMet: 1.534 ± 0.05	1.92GlnAsn: 1.92 ± 0.06	1.026GlnPro: 1.026 ± 0.038	1.655GlnGln: 1.655 ± 0.069	1.578GlnArg: 1.578 ± 0.053	2.14GlnSer: 2.14 ± 0.048	2.208GlnThr: 2.208 ± 0.061	2.269GlnVal: 2.269 ± 0.054	0.385GlnTrp: 0.385 ± 0.027	1.78GlnTyr: 1.78 ± 0.057	0.003GlnXaa: 0.003 ± 0.003
Arg	2.708ArgAla: 2.708 ± 0.064	0.535ArgCys: 0.535 ± 0.027	2.328ArgAsp: 2.328 ± 0.058	3.532ArgGlu: 3.532 ± 0.095	1.814ArgPhe: 1.814 ± 0.059	2.592ArgGly: 2.592 ± 0.068	0.873ArgHis: 0.873 ± 0.036	3.556ArgIle: 3.556 ± 0.072	3.602ArgLys: 3.602 ± 0.074	3.721ArgLeu: 3.721 ± 0.084	1.599ArgMet: 1.599 ± 0.057	1.937ArgAsn: 1.937 ± 0.052	1.282ArgPro: 1.282 ± 0.041	1.966ArgGln: 1.966 ± 0.059	2.293ArgArg: 2.293 ± 0.057	2.403ArgSer: 2.403 ± 0.061	2.299ArgThr: 2.299 ± 0.05	2.833ArgVal: 2.833 ± 0.064	0.397ArgTrp: 0.397 ± 0.028	2.008ArgTyr: 2.008 ± 0.064	0.009ArgXaa: 0.009 ± 0.003
Ser	4.489SerAla: 4.489 ± 0.093	0.766SerCys: 0.766 ± 0.03	3.514SerAsp: 3.514 ± 0.08	3.872SerGlu: 3.872 ± 0.079	2.467SerPhe: 2.467 ± 0.062	4.779SerGly: 4.779 ± 0.095	1.013SerHis: 1.013 ± 0.04	4.069SerIle: 4.069 ± 0.087	4.073SerLys: 4.073 ± 0.091	5.108SerLeu: 5.108 ± 0.098	1.746SerMet: 1.746 ± 0.048	2.448SerAsn: 2.448 ± 0.069	1.565SerPro: 1.565 ± 0.048	1.832SerGln: 1.832 ± 0.057	2.53SerArg: 2.53 ± 0.059	3.9SerSer: 3.9 ± 0.115	3.158SerThr: 3.158 ± 0.074	4.375SerVal: 4.375 ± 0.093	0.43SerTrp: 0.43 ± 0.027	2.598SerTyr: 2.598 ± 0.067	0.013SerXaa: 0.013 ± 0.005
Thr	4.58ThrAla: 4.58 ± 0.098	0.684ThrCys: 0.684 ± 0.036	3.288ThrAsp: 3.288 ± 0.083	3.64ThrGlu: 3.64 ± 0.076	2.117ThrPhe: 2.117 ± 0.054	4.383ThrGly: 4.383 ± 0.084	0.941ThrHis: 0.941 ± 0.04	4.301ThrIle: 4.301 ± 0.087	4.157ThrLys: 4.157 ± 0.091	4.936ThrLeu: 4.936 ± 0.093	1.599ThrMet: 1.599 ± 0.042	2.252ThrAsn: 2.252 ± 0.063	2.205ThrPro: 2.205 ± 0.055	1.751ThrGln: 1.751 ± 0.047	1.902ThrArg: 1.902 ± 0.049	3.245ThrSer: 3.245 ± 0.069	3.239ThrThr: 3.239 ± 0.083	4.741ThrVal: 4.741 ± 0.12	0.45ThrTrp: 0.45 ± 0.026	2.148ThrTyr: 2.148 ± 0.062	0.014ThrXaa: 0.014 ± 0.004
Val	5.19ValAla: 5.19 ± 0.107	1.248ValCys: 1.248 ± 0.041	4.002ValAsp: 4.002 ± 0.083	4.33ValGlu: 4.33 ± 0.093	2.773ValPhe: 2.773 ± 0.07	4.339ValGly: 4.339 ± 0.097	1.221ValHis: 1.221 ± 0.041	5.29ValIle: 5.29 ± 0.101	4.763ValLys: 4.763 ± 0.103	6.681ValLeu: 6.681 ± 0.12	2.006ValMet: 2.006 ± 0.059	2.872ValAsn: 2.872 ± 0.067	2.367ValPro: 2.367 ± 0.062	2.478ValGln: 2.478 ± 0.059	2.939ValArg: 2.939 ± 0.072	4.903ValSer: 4.903 ± 0.098	4.561ValThr: 4.561 ± 0.103	5.047ValVal: 5.047 ± 0.109	0.652ValTrp: 0.652 ± 0.033	2.894ValTyr: 2.894 ± 0.067	0.007ValXaa: 0.007 ± 0.003
Trp	0.416TrpAla: 0.416 ± 0.025	0.13TrpCys: 0.13 ± 0.014	0.515TrpAsp: 0.515 ± 0.031	0.58TrpGlu: 0.58 ± 0.03	0.369TrpPhe: 0.369 ± 0.025	0.625TrpGly: 0.625 ± 0.032	0.192TrpHis: 0.192 ± 0.017	0.598TrpIle: 0.598 ± 0.028	0.738TrpLys: 0.738 ± 0.04	0.781TrpLeu: 0.781 ± 0.036	0.302TrpMet: 0.302 ± 0.025	0.524TrpAsn: 0.524 ± 0.031	0.185TrpPro: 0.185 ± 0.016	0.424TrpGln: 0.424 ± 0.024	0.369TrpArg: 0.369 ± 0.026	0.507TrpSer: 0.507 ± 0.031	0.449TrpThr: 0.449 ± 0.028	0.456TrpVal: 0.456 ± 0.025	0.118TrpTrp: 0.118 ± 0.013	0.398TrpTyr: 0.398 ± 0.025	0.003TrpXaa: 0.003 ± 0.002
Tyr	2.983TyrAla: 2.983 ± 0.075	0.707TyrCys: 0.707 ± 0.036	2.739TyrAsp: 2.739 ± 0.066	2.954TyrGlu: 2.954 ± 0.071	1.856TyrPhe: 1.856 ± 0.055	2.941TyrGly: 2.941 ± 0.065	1.006TyrHis: 1.006 ± 0.035	2.833TyrIle: 2.833 ± 0.06	2.324TyrLys: 2.324 ± 0.066	3.741TyrLeu: 3.741 ± 0.079	1.181TyrMet: 1.181 ± 0.047	1.755TyrAsn: 1.755 ± 0.062	1.459TyrPro: 1.459 ± 0.044	1.79TyrGln: 1.79 ± 0.051	2.279TyrArg: 2.279 ± 0.062	2.467TyrSer: 2.467 ± 0.063	2.347TyrThr: 2.347 ± 0.067	2.806TyrVal: 2.806 ± 0.069	0.338TyrTrp: 0.338 ± 0.023	2.143TyrTyr: 2.143 ± 0.059	0.004TyrXaa: 0.004 ± 0.003
Xaa	0.012XaaAla: 0.012 ± 0.004	0.001XaaCys: 0.001 ± 0.002	0.012XaaAsp: 0.012 ± 0.004	0.001XaaGlu: 0.001 ± 0.001	0.006XaaPhe: 0.006 ± 0.003	0.013XaaGly: 0.013 ± 0.004	0.001XaaHis: 0.001 ± 0.001	0.013XaaIle: 0.013 ± 0.004	0.003XaaLys: 0.003 ± 0.002	0.007XaaLeu: 0.007 ± 0.004	0.007XaaMet: 0.007 ± 0.004	0.007XaaAsn: 0.007 ± 0.003	0.007XaaPro: 0.007 ± 0.003	0.001XaaGln: 0.001 ± 0.002	0.014XaaArg: 0.014 ± 0.004	0.019XaaSer: 0.019 ± 0.005	0.009XaaThr: 0.009 ± 0.004	0.009XaaVal: 0.009 ± 0.003	0.001XaaTrp: 0.001 ± 0.002	0.001XaaTyr: 0.001 ± 0.002	0.167XaaXaa: 0.167 ± 0.02

Statistics based on 2286 proteins (692856 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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