Kocuria sp. HSID16901
Average proteome isoelectric point is 6.03
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2178 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A433H2H6|A0A433H2H6_9MICC ATP-sulfurylase large subunit OS=Kocuria sp. HSID16901 OX=2419505 GN=D8M21_03535 PE=4 SV=1
MM1 pKa = 7.44 ARR3 pKa = 11.84 ALLIVDD9 pKa = 4.02 VQNDD13 pKa = 3.76 FCRR16 pKa = 11.84 GGALATDD23 pKa = 3.98 RR24 pKa = 11.84 GDD26 pKa = 3.98 DD27 pKa = 3.49 VAAEE31 pKa = 4.15 ISAYY35 pKa = 10.95 LLTHH39 pKa = 6.3 HH40 pKa = 7.26 AAYY43 pKa = 10.31 DD44 pKa = 3.94 VVVTTQDD51 pKa = 2.65 WHH53 pKa = 6.83 IDD55 pKa = 3.48 PGTHH59 pKa = 6.48 FSEE62 pKa = 5.18 NPDD65 pKa = 3.64 FTNSWPVHH73 pKa = 5.38 CVANSSGAAFHH84 pKa = 7.55 PDD86 pKa = 3.07 LDD88 pKa = 3.8 MDD90 pKa = 4.11 YY91 pKa = 10.98 VDD93 pKa = 4.53 AMFRR97 pKa = 11.84 KK98 pKa = 10.15 GEE100 pKa = 3.99 YY101 pKa = 8.44 EE102 pKa = 3.52 AAYY105 pKa = 10.68 SGFEE109 pKa = 3.94 GLLAPEE115 pKa = 4.69 DD116 pKa = 3.66 SVMTGEE122 pKa = 4.74 HH123 pKa = 6.52 EE124 pKa = 4.47 PGHH127 pKa = 5.75 MPEE130 pKa = 4.54 DD131 pKa = 3.89 TLKK134 pKa = 11.09 VSLDD138 pKa = 3.44 DD139 pKa = 3.75 WLQEE143 pKa = 3.96 QDD145 pKa = 3.58 VTDD148 pKa = 3.63 VDD150 pKa = 4.33 VVGIATDD157 pKa = 3.55 YY158 pKa = 10.74 CVRR161 pKa = 11.84 ATALDD166 pKa = 3.89 AADD169 pKa = 3.97 AGYY172 pKa = 7.43 EE173 pKa = 4.08 TRR175 pKa = 11.84 VLVDD179 pKa = 3.65 LTSAVADD186 pKa = 4.13 EE187 pKa = 4.32 TAEE190 pKa = 4.04 TAVAEE195 pKa = 4.3 LEE197 pKa = 4.27 DD198 pKa = 4.63 AGVQVIEE205 pKa = 4.38 STSVGRR211 pKa = 4.29
Molecular weight: 22.76 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.745
IPC2_protein 3.884
IPC_protein 3.884
Toseland 3.668
ProMoST 4.05
Dawson 3.884
Bjellqvist 4.037
Wikipedia 3.808
Rodwell 3.719
Grimsley 3.579
Solomon 3.872
Lehninger 3.834
Nozaki 3.986
DTASelect 4.228
Thurlkill 3.719
EMBOSS 3.821
Sillero 4.012
Patrickios 1.914
IPC_peptide 3.872
IPC2_peptide 3.986
IPC2.peptide.svr19 3.901
Protein with the highest isoelectric point:
>tr|A0A433GWA0|A0A433GWA0_9MICC Ribonuclease P protein component OS=Kocuria sp. HSID16901 OX=2419505 GN=rnpA PE=3 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.0 RR4 pKa = 11.84 TFQPNNRR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 AKK15 pKa = 9.97 KK16 pKa = 9.33 HH17 pKa = 4.25 GFRR20 pKa = 11.84 LRR22 pKa = 11.84 MRR24 pKa = 11.84 TRR26 pKa = 11.84 AGRR29 pKa = 11.84 AILANRR35 pKa = 11.84 RR36 pKa = 11.84 AKK38 pKa = 10.48 GRR40 pKa = 11.84 ASVSAA45 pKa = 3.98
Molecular weight: 5.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.286
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.676
Grimsley 13.086
Solomon 13.539
Lehninger 13.437
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.398
IPC_peptide 13.539
IPC2_peptide 12.53
IPC2.peptide.svr19 9.215
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2178
0
2178
733174
29
2179
336.6
36.5
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.986 ± 0.057
0.651 ± 0.013
6.03 ± 0.046
6.43 ± 0.065
3.172 ± 0.035
8.651 ± 0.055
2.336 ± 0.022
4.767 ± 0.033
3.047 ± 0.04
9.378 ± 0.056
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.232 ± 0.023
2.663 ± 0.029
5.098 ± 0.034
3.34 ± 0.029
6.773 ± 0.061
6.693 ± 0.04
5.953 ± 0.035
8.204 ± 0.037
1.406 ± 0.023
2.19 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here