Longjawed orbweaver circular virus 1
Average proteome isoelectric point is 7.95
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A346BPA7|A0A346BPA7_9VIRU Putative capsid protein OS=Longjawed orbweaver circular virus 1 OX=2293294 PE=4 SV=1
MM1 pKa = 7.4 SRR3 pKa = 11.84 QKK5 pKa = 10.11 GWCYY9 pKa = 10.06 TRR11 pKa = 11.84 FDD13 pKa = 3.7 IEE15 pKa = 4.7 NIPVYY20 pKa = 10.45 NEE22 pKa = 4.06 HH23 pKa = 6.86 EE24 pKa = 4.12 EE25 pKa = 4.35 SYY27 pKa = 10.73 HH28 pKa = 6.31 IFGRR32 pKa = 11.84 EE33 pKa = 3.65 RR34 pKa = 11.84 CPSTGRR40 pKa = 11.84 IHH42 pKa = 5.79 YY43 pKa = 10.22 QGFIYY48 pKa = 9.91 FKK50 pKa = 9.58 QRR52 pKa = 11.84 KK53 pKa = 8.91 RR54 pKa = 11.84 LTQLKK59 pKa = 8.72 KK60 pKa = 10.55 VGYY63 pKa = 7.81 TGHH66 pKa = 6.56 FEE68 pKa = 4.24 AMRR71 pKa = 11.84 GSPQQASEE79 pKa = 4.29 YY80 pKa = 9.66 CKK82 pKa = 10.35 KK83 pKa = 10.84 DD84 pKa = 2.79 SDD86 pKa = 4.03 YY87 pKa = 11.71 TEE89 pKa = 5.42 FGQLPVVSSGGSVFADD105 pKa = 3.46 ALAKK109 pKa = 10.59 AKK111 pKa = 10.82 AGDD114 pKa = 3.58 FDD116 pKa = 5.9 SIEE119 pKa = 4.18 EE120 pKa = 3.97 NHH122 pKa = 6.81 PGVFLRR128 pKa = 11.84 YY129 pKa = 9.49 RR130 pKa = 11.84 GTLLSCFKK138 pKa = 10.89 YY139 pKa = 10.83 NVVEE143 pKa = 4.2 LLNSCGVWICGPPRR157 pKa = 11.84 SGKK160 pKa = 10.17 DD161 pKa = 3.15 YY162 pKa = 11.03 SVRR165 pKa = 11.84 KK166 pKa = 9.87 LSSLYY171 pKa = 10.13 IKK173 pKa = 10.02 PLNKK177 pKa = 9.22 WWDD180 pKa = 3.51 GYY182 pKa = 9.8 MNEE185 pKa = 4.42 DD186 pKa = 3.31 NVLISDD192 pKa = 3.8 VDD194 pKa = 3.91 VSHH197 pKa = 6.68 GNWLGYY203 pKa = 8.57 FLKK206 pKa = 10.23 IWMDD210 pKa = 3.74 RR211 pKa = 11.84 YY212 pKa = 11.21 AFIGEE217 pKa = 4.2 IKK219 pKa = 10.43 GGSIKK224 pKa = 10.03 IRR226 pKa = 11.84 PKK228 pKa = 10.56 KK229 pKa = 10.79 VFVTSNFLIEE239 pKa = 4.37 DD240 pKa = 3.72 VFVNEE245 pKa = 4.07 QVCAAVKK252 pKa = 10.12 ARR254 pKa = 11.84 CNIYY258 pKa = 10.7 NLFEE262 pKa = 4.26 HH263 pKa = 6.35 VVTRR267 pKa = 11.84 RR268 pKa = 11.84 VDD270 pKa = 3.35 INPSDD275 pKa = 4.5 DD276 pKa = 3.64 LFKK279 pKa = 11.14 LLEE282 pKa = 4.27 EE283 pKa = 4.7 NGDD286 pKa = 3.84 VVQTSTRR293 pKa = 11.84 APLSEE298 pKa = 4.06 VQAEE302 pKa = 4.58 VVAGPSGHH310 pKa = 6.25 VPPKK314 pKa = 9.99 KK315 pKa = 8.63 KK316 pKa = 10.54 CKK318 pKa = 8.51 VAEE321 pKa = 4.18 VFSDD325 pKa = 4.0 SEE327 pKa = 4.26 DD328 pKa = 3.81 FEE330 pKa = 4.65
Molecular weight: 37.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.634
IPC2_protein 6.795
IPC_protein 6.825
Toseland 6.678
ProMoST 7.146
Dawson 7.439
Bjellqvist 7.556
Wikipedia 7.366
Rodwell 7.439
Grimsley 6.751
Solomon 7.483
Lehninger 7.512
Nozaki 7.849
DTASelect 7.644
Thurlkill 7.673
EMBOSS 7.717
Sillero 7.922
Patrickios 4.101
IPC_peptide 7.483
IPC2_peptide 7.132
IPC2.peptide.svr19 7.107
Protein with the highest isoelectric point:
>tr|A0A346BPA7|A0A346BPA7_9VIRU Putative capsid protein OS=Longjawed orbweaver circular virus 1 OX=2293294 PE=4 SV=1
MM1 pKa = 7.71 LFRR4 pKa = 11.84 RR5 pKa = 11.84 RR6 pKa = 11.84 LGLRR10 pKa = 11.84 YY11 pKa = 9.13 RR12 pKa = 11.84 RR13 pKa = 11.84 YY14 pKa = 9.86 RR15 pKa = 11.84 RR16 pKa = 11.84 KK17 pKa = 9.32 SWPARR22 pKa = 11.84 RR23 pKa = 11.84 AMYY26 pKa = 9.96 RR27 pKa = 11.84 RR28 pKa = 11.84 RR29 pKa = 11.84 RR30 pKa = 11.84 SAKK33 pKa = 8.79 LRR35 pKa = 11.84 KK36 pKa = 8.88 SSRR39 pKa = 11.84 IRR41 pKa = 11.84 RR42 pKa = 11.84 ISNDD46 pKa = 3.03 YY47 pKa = 9.68 TKK49 pKa = 10.06 EE50 pKa = 4.13 TFSDD54 pKa = 3.12 VTMYY58 pKa = 11.27 VLLPDD63 pKa = 3.41 KK64 pKa = 10.66 VKK66 pKa = 10.66 PEE68 pKa = 4.3 RR69 pKa = 11.84 PSSTSFEE76 pKa = 4.38 MFTSAIGTDD85 pKa = 3.34 SMNSDD90 pKa = 3.28 RR91 pKa = 11.84 FGFLCTVYY99 pKa = 10.41 KK100 pKa = 10.14 YY101 pKa = 11.41 VKK103 pKa = 9.59 FNSFVWHH110 pKa = 5.27 VTCDD114 pKa = 3.99 PITAVSGAFAVEE126 pKa = 3.92 KK127 pKa = 9.63 TVVKK131 pKa = 9.63 GTVSGLNEE139 pKa = 3.81 FFKK142 pKa = 11.55 NEE144 pKa = 3.91 DD145 pKa = 4.33 FYY147 pKa = 12.15 VSWDD151 pKa = 3.39 LNFAYY156 pKa = 10.07 DD157 pKa = 3.44 ASKK160 pKa = 11.07 AITVKK165 pKa = 10.75 DD166 pKa = 3.34 LTGIHH171 pKa = 6.44 AKK173 pKa = 7.98 KK174 pKa = 8.86 TRR176 pKa = 11.84 IGGHH180 pKa = 5.92 KK181 pKa = 9.62 PAIFKK186 pKa = 10.12 WKK188 pKa = 9.88 VPQKK192 pKa = 9.82 LRR194 pKa = 11.84 EE195 pKa = 3.94 YY196 pKa = 9.18 VLCSEE201 pKa = 4.55 VPDD204 pKa = 3.73 ARR206 pKa = 11.84 SGNVPIGSFFNNLLEE221 pKa = 4.45 RR222 pKa = 11.84 KK223 pKa = 9.5 NINVPNYY230 pKa = 10.24 FIGCGTEE237 pKa = 3.78 IMRR240 pKa = 11.84 KK241 pKa = 9.4 LPNFNNMFDD250 pKa = 3.66 DD251 pKa = 3.48 VRR253 pKa = 11.84 FVLRR257 pKa = 11.84 VNCFVNCTFHH267 pKa = 6.91 GAVILKK273 pKa = 9.13 QQ274 pKa = 3.26
Molecular weight: 31.9 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.268
IPC2_protein 9.531
IPC_protein 9.736
Toseland 10.321
ProMoST 9.955
Dawson 10.467
Bjellqvist 10.131
Wikipedia 10.628
Rodwell 10.877
Grimsley 10.526
Solomon 10.496
Lehninger 10.467
Nozaki 10.321
DTASelect 10.116
Thurlkill 10.335
EMBOSS 10.701
Sillero 10.379
Patrickios 10.54
IPC_peptide 10.511
IPC2_peptide 8.975
IPC2.peptide.svr19 8.543
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
604
274
330
302.0
34.78
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.801 ± 0.19
2.483 ± 0.181
5.298 ± 0.341
5.464 ± 1.119
6.788 ± 0.766
6.457 ± 0.831
1.987 ± 0.325
4.967 ± 0.137
7.781 ± 0.153
6.291 ± 0.054
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.821 ± 0.452
5.298 ± 0.559
4.139 ± 0.077
1.987 ± 0.775
7.45 ± 1.483
7.616 ± 0.195
4.305 ± 0.947
8.94 ± 0.112
1.656 ± 0.121
4.47 ± 0.281
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here