Corynebacterium tapiri
Average proteome isoelectric point is 5.94
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2045 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5C4U296|A0A5C4U296_9CORY Mak_N_cap domain-containing protein OS=Corynebacterium tapiri OX=1448266 GN=FHE74_10075 PE=4 SV=1
MM1 pKa = 7.31 KK2 pKa = 10.46 CEE4 pKa = 4.08 PTVTLAVCCALALSGCSTLDD24 pKa = 3.58 DD25 pKa = 4.04 VPVPAEE31 pKa = 4.88 DD32 pKa = 3.84 GTPTASASAPDD43 pKa = 3.89 SAPAPEE49 pKa = 3.99 QAPALGADD57 pKa = 4.09 LPVDD61 pKa = 3.76 TRR63 pKa = 11.84 PVGQRR68 pKa = 11.84 TNFNEE73 pKa = 4.14 CPYY76 pKa = 11.23 LDD78 pKa = 4.21 TQWVADD84 pKa = 3.89 TNGQRR89 pKa = 11.84 VTGVDD94 pKa = 2.72 VDD96 pKa = 3.99 TRR98 pKa = 11.84 FDD100 pKa = 3.74 PPACVYY106 pKa = 9.98 FSYY109 pKa = 10.98 PEE111 pKa = 4.17 APQLEE116 pKa = 4.95 VIVRR120 pKa = 11.84 HH121 pKa = 5.68 MPTEE125 pKa = 4.16 ADD127 pKa = 3.71 AIAVVDD133 pKa = 4.11 TAAPISTTEE142 pKa = 4.33 PADD145 pKa = 4.0 QPAGWMGGRR154 pKa = 11.84 AGEE157 pKa = 4.2 GMVPGVEE164 pKa = 3.92 GSVYY168 pKa = 10.55 AVQKK172 pKa = 10.6 GKK174 pKa = 8.22 TAVVVYY180 pKa = 10.14 SNQGQSFKK188 pKa = 10.56 PEE190 pKa = 3.71 QVAGQVINNLGLL202 pKa = 3.54
Molecular weight: 20.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.807
IPC2_protein 4.037
IPC_protein 3.999
Toseland 3.795
ProMoST 4.139
Dawson 3.973
Bjellqvist 4.126
Wikipedia 3.897
Rodwell 3.821
Grimsley 3.706
Solomon 3.961
Lehninger 3.923
Nozaki 4.088
DTASelect 4.304
Thurlkill 3.834
EMBOSS 3.91
Sillero 4.113
Patrickios 1.926
IPC_peptide 3.961
IPC2_peptide 4.088
IPC2.peptide.svr19 3.995
Protein with the highest isoelectric point:
>tr|A0A5C4U3X9|A0A5C4U3X9_9CORY Mannose-6-phosphate isomerase OS=Corynebacterium tapiri OX=1448266 GN=manA PE=3 SV=1
MM1 pKa = 7.69 AKK3 pKa = 10.06 GKK5 pKa = 8.69 RR6 pKa = 11.84 TFQPNNRR13 pKa = 11.84 RR14 pKa = 11.84 RR15 pKa = 11.84 ARR17 pKa = 11.84 KK18 pKa = 8.59 HH19 pKa = 4.58 GFRR22 pKa = 11.84 TRR24 pKa = 11.84 MRR26 pKa = 11.84 TRR28 pKa = 11.84 AGRR31 pKa = 11.84 AIVSARR37 pKa = 11.84 RR38 pKa = 11.84 KK39 pKa = 9.63 KK40 pKa = 10.53 GRR42 pKa = 11.84 ASLTAA47 pKa = 4.1
Molecular weight: 5.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.316
IPC_protein 12.925
Toseland 13.086
ProMoST 13.583
Dawson 13.086
Bjellqvist 13.086
Wikipedia 13.568
Rodwell 12.735
Grimsley 13.13
Solomon 13.583
Lehninger 13.481
Nozaki 13.086
DTASelect 13.086
Thurlkill 13.086
EMBOSS 13.583
Sillero 13.086
Patrickios 12.457
IPC_peptide 13.583
IPC2_peptide 12.574
IPC2.peptide.svr19 9.237
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2045
0
2045
685056
33
2986
335.0
36.21
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.905 ± 0.066
0.706 ± 0.016
5.908 ± 0.046
6.347 ± 0.054
3.237 ± 0.03
8.377 ± 0.045
2.268 ± 0.03
4.807 ± 0.037
2.982 ± 0.044
9.652 ± 0.058
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.134 ± 0.025
2.732 ± 0.033
5.106 ± 0.046
3.463 ± 0.027
6.357 ± 0.052
6.053 ± 0.038
5.988 ± 0.045
8.457 ± 0.048
1.338 ± 0.021
2.183 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here