Microbacterium maritypicum MF109

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Microbacterium; Microbacterium maritypicum

Average proteome isoelectric point is 5.94

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3856 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|T5KVZ8|T5KVZ8_9MICO DUF305 domain-containing protein OS=Microbacterium maritypicum MF109 OX=1333857 GN=L687_11630 PE=4 SV=1
MM1 pKa = 7.74SIPEE5 pKa = 4.1PTQTPDD11 pKa = 2.8GPAYY15 pKa = 10.01EE16 pKa = 4.71GRR18 pKa = 11.84LLDD21 pKa = 4.06RR22 pKa = 11.84VDD24 pKa = 4.28EE25 pKa = 4.31EE26 pKa = 5.3VVDD29 pKa = 3.91QGAAFDD35 pKa = 3.91IRR37 pKa = 11.84TLLSRR42 pKa = 11.84RR43 pKa = 11.84GVLGLAGIGLGAVVLAACAPAASGGSTSAATPSATAGTSSGDD85 pKa = 3.35VAGVGEE91 pKa = 4.87IPDD94 pKa = 3.99EE95 pKa = 4.32TAGPYY100 pKa = 10.2PGDD103 pKa = 3.77GSNGPDD109 pKa = 3.29VLEE112 pKa = 4.52DD113 pKa = 3.47AGIVRR118 pKa = 11.84QDD120 pKa = 2.86IRR122 pKa = 11.84SSIDD126 pKa = 2.81GGATADD132 pKa = 3.82GVPLTFEE139 pKa = 4.2LQVFDD144 pKa = 4.65LANGGVPFAGVAVYY158 pKa = 9.76AWHH161 pKa = 6.68CTAQGGYY168 pKa = 10.69SMYY171 pKa = 10.86SSGLEE176 pKa = 3.76DD177 pKa = 3.12VTYY180 pKa = 11.0LRR182 pKa = 11.84GVQVADD188 pKa = 3.32ADD190 pKa = 4.03GRR192 pKa = 11.84VSFTSVFPGCYY203 pKa = 8.96SGRR206 pKa = 11.84WPHH209 pKa = 6.02IHH211 pKa = 6.47FEE213 pKa = 4.29VYY215 pKa = 10.19PDD217 pKa = 3.38VGSITDD223 pKa = 3.48STAAIATSQLALPEE237 pKa = 4.19AACTDD242 pKa = 3.96VYY244 pKa = 11.66AEE246 pKa = 3.96AAYY249 pKa = 10.46DD250 pKa = 4.07GSASNLAQITLASDD264 pKa = 3.42NVFGDD269 pKa = 4.23DD270 pKa = 4.3SAALQLAAVTGSVRR284 pKa = 11.84DD285 pKa = 4.68GYY287 pKa = 11.48AGTLVVGVDD296 pKa = 3.48TATAPAMSGGQGGPGGGGQPPSNN319 pKa = 3.75

Molecular weight: 31.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|T5L6A2|T5L6A2_9MICO ABC transporter domain-containing protein OS=Microbacterium maritypicum MF109 OX=1333857 GN=L687_10335 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.56LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3856

0

3856

1203482

31

2029

312.1

33.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.457 ± 0.052

0.482 ± 0.01

6.291 ± 0.039

5.718 ± 0.035

3.174 ± 0.026

8.858 ± 0.032

1.931 ± 0.022

4.871 ± 0.033

1.982 ± 0.029

10.079 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.881 ± 0.017

1.938 ± 0.021

5.274 ± 0.029

2.78 ± 0.022

7.149 ± 0.043

5.642 ± 0.023

6.188 ± 0.039

8.862 ± 0.033

1.529 ± 0.02

1.913 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski