Shahe hepe-like virus 2

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KK59|A0A1L3KK59_9VIRU Alphavirus-like MT domain-containing protein OS=Shahe hepe-like virus 2 OX=1923416 PE=4 SV=1
MM1 pKa = 7.33NFDD4 pKa = 5.02LIEE7 pKa = 4.44DD8 pKa = 3.97VVGDD12 pKa = 3.43VMNIYY17 pKa = 10.1PEE19 pKa = 4.14QPIHH23 pKa = 6.07ARR25 pKa = 11.84AMIKK29 pKa = 10.05RR30 pKa = 11.84HH31 pKa = 4.77FTLEE35 pKa = 3.83KK36 pKa = 10.75LGDD39 pKa = 3.84HH40 pKa = 7.0AAGKK44 pKa = 9.85YY45 pKa = 7.18WLIPITFQEE54 pKa = 4.41PDD56 pKa = 2.87QHH58 pKa = 8.32AEE60 pKa = 3.57YY61 pKa = 10.48MMYY64 pKa = 10.44RR65 pKa = 11.84ISNYY69 pKa = 9.75KK70 pKa = 10.57LEE72 pKa = 4.41FNNTKK77 pKa = 10.05HH78 pKa = 5.46QMNVSTTASNNNDD91 pKa = 3.02GYY93 pKa = 11.36FRR95 pKa = 11.84IAWIRR100 pKa = 11.84DD101 pKa = 3.47PMIGTPKK108 pKa = 10.64DD109 pKa = 3.31YY110 pKa = 10.77MNFVTRR116 pKa = 11.84LPGSKK121 pKa = 9.92LVSWYY126 pKa = 10.46DD127 pKa = 3.35NLSFQVEE134 pKa = 4.6TNGWRR139 pKa = 11.84YY140 pKa = 9.26CKK142 pKa = 10.03KK143 pKa = 10.8GKK145 pKa = 9.96DD146 pKa = 3.86KK147 pKa = 11.17RR148 pKa = 11.84LTDD151 pKa = 3.57YY152 pKa = 11.65GDD154 pKa = 3.66IIIAKK159 pKa = 9.99FSYY162 pKa = 9.89TGLDD166 pKa = 3.95GVWTEE171 pKa = 4.47SFDD174 pKa = 3.64IEE176 pKa = 4.64YY177 pKa = 10.48SVPTLPSKK185 pKa = 7.86WTPITCYY192 pKa = 10.96ALDD195 pKa = 3.63TLAAAGAADD204 pKa = 4.12YY205 pKa = 11.29KK206 pKa = 11.28DD207 pKa = 4.31LLSFTASYY215 pKa = 10.99SGAQQLYY222 pKa = 10.0CNFKK226 pKa = 10.79LGLEE230 pKa = 4.16KK231 pKa = 10.77GAIPTQKK238 pKa = 10.1KK239 pKa = 10.29GRR241 pKa = 11.84ITLGKK246 pKa = 8.8PVVIALIFTATPVSGDD262 pKa = 3.78PYY264 pKa = 10.69DD265 pKa = 3.56VTYY268 pKa = 10.31EE269 pKa = 4.16FPFTSAEE276 pKa = 4.18CEE278 pKa = 4.34SIGTGLWGGNLDD290 pKa = 4.81ASLLDD295 pKa = 2.99IEE297 pKa = 5.92FYY299 pKa = 10.67INPYY303 pKa = 10.07GEE305 pKa = 4.67LGADD309 pKa = 3.55VLSLKK314 pKa = 10.33SVYY317 pKa = 9.33WDD319 pKa = 3.17QDD321 pKa = 3.6PNISADD327 pKa = 3.11EE328 pKa = 4.09RR329 pKa = 11.84MSLKK333 pKa = 10.6AQLIFAEE340 pKa = 4.67EE341 pKa = 4.42VPDD344 pKa = 5.01SINDD348 pKa = 4.17LIEE351 pKa = 4.04TLNIGAGSAYY361 pKa = 9.74EE362 pKa = 4.18DD363 pKa = 4.53LVNKK367 pKa = 9.4YY368 pKa = 9.85

Molecular weight: 41.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KK79|A0A1L3KK79_9VIRU Uncharacterized protein OS=Shahe hepe-like virus 2 OX=1923416 PE=4 SV=1
MM1 pKa = 7.55SKK3 pKa = 10.84GFTLDD8 pKa = 2.63SYY10 pKa = 11.7YY11 pKa = 11.26LFLPLSFLGAKK22 pKa = 9.76FCLVLIFQSRR32 pKa = 11.84FVSVISKK39 pKa = 9.4FISLSLFYY47 pKa = 11.34VNFSSCQSSTTGFSLICYY65 pKa = 9.87SEE67 pKa = 5.51AILDD71 pKa = 4.02LSWIISISTLRR82 pKa = 11.84LPTEE86 pKa = 4.0CWMVQHH92 pKa = 7.24APHH95 pKa = 5.96QQPKK99 pKa = 8.51CTSTNFGMTSTQTGYY114 pKa = 10.15PKK116 pKa = 11.03LLTCHH121 pKa = 6.22GRR123 pKa = 11.84WTMKK127 pKa = 10.58LL128 pKa = 3.32

Molecular weight: 14.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

3403

128

2173

567.2

64.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.464 ± 0.414

1.822 ± 0.355

5.877 ± 0.734

6.142 ± 0.533

5.73 ± 0.806

5.348 ± 0.357

2.557 ± 0.497

7.141 ± 0.257

7.229 ± 0.63

7.787 ± 0.656

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.056 ± 0.209

4.731 ± 0.282

4.731 ± 0.609

3.438 ± 0.403

3.879 ± 0.515

5.789 ± 1.093

6.759 ± 0.357

5.671 ± 0.29

1.058 ± 0.281

3.791 ± 0.55
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski