Hubei permutotetra-like virus 6
Average proteome isoelectric point is 6.91
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KHW2|A0A1L3KHW2_9VIRU Putative capsid protein OS=Hubei permutotetra-like virus 6 OX=1923080 PE=4 SV=1
MM1 pKa = 7.59 DD2 pKa = 4.07 TSNPVVSSDD11 pKa = 2.99 RR12 pKa = 11.84 RR13 pKa = 11.84 SLRR16 pKa = 11.84 EE17 pKa = 3.85 AKK19 pKa = 10.01 EE20 pKa = 3.46 LAVVKK25 pKa = 10.31 KK26 pKa = 10.43 RR27 pKa = 11.84 QDD29 pKa = 2.95 LEE31 pKa = 4.4 YY32 pKa = 10.58 IMNVAQTSTRR42 pKa = 11.84 VILPVGEE49 pKa = 4.21 RR50 pKa = 11.84 ALPEE54 pKa = 4.34 AEE56 pKa = 4.2 VKK58 pKa = 10.74 SIILNLKK65 pKa = 10.09 DD66 pKa = 3.51 KK67 pKa = 10.35 EE68 pKa = 4.41 PTVRR72 pKa = 11.84 TDD74 pKa = 3.33 SEE76 pKa = 4.49 DD77 pKa = 3.52 MLDD80 pKa = 3.33 EE81 pKa = 4.44 TVFQLVAPSMPVCGLLSQDD100 pKa = 4.22 GVPLHH105 pKa = 6.73 PPPIHH110 pKa = 5.75 NAKK113 pKa = 10.25 GRR115 pKa = 11.84 VTTNRR120 pKa = 11.84 RR121 pKa = 11.84 FGGGAYY127 pKa = 9.14 KK128 pKa = 10.51 PLGIRR133 pKa = 11.84 PMVEE137 pKa = 3.1 ILGVRR142 pKa = 11.84 TQEE145 pKa = 3.42 ITKK148 pKa = 9.99 VYY150 pKa = 8.91 THH152 pKa = 6.38 GTWQGFSNRR161 pKa = 11.84 LAKK164 pKa = 10.46 QMGRR168 pKa = 11.84 KK169 pKa = 8.06 CVSMGMTIRR178 pKa = 11.84 NSMPGVQMDD187 pKa = 3.58 KK188 pKa = 9.47 NTPRR192 pKa = 11.84 RR193 pKa = 11.84 IFFDD197 pKa = 3.06 ILNKK201 pKa = 10.16 KK202 pKa = 8.63 MPARR206 pKa = 11.84 KK207 pKa = 8.83 PSGWPSLSDD216 pKa = 3.79 PLDD219 pKa = 4.13 TILDD223 pKa = 4.16 NIKK226 pKa = 8.76 ITLDD230 pKa = 3.26 ASAGPPYY237 pKa = 9.5 WRR239 pKa = 11.84 NKK241 pKa = 8.22 VEE243 pKa = 5.39 CMDD246 pKa = 4.08 QILSEE251 pKa = 4.66 GIPLLVKK258 pKa = 10.25 ALKK261 pKa = 10.19 EE262 pKa = 3.87 NRR264 pKa = 11.84 LEE266 pKa = 3.87 QFMRR270 pKa = 11.84 EE271 pKa = 3.8 EE272 pKa = 4.59 PEE274 pKa = 4.24 FFLCEE279 pKa = 4.11 VKK281 pKa = 10.95 NKK283 pKa = 9.89 LDD285 pKa = 3.5 RR286 pKa = 11.84 YY287 pKa = 10.37 GVDD290 pKa = 3.51 EE291 pKa = 5.75 LDD293 pKa = 3.92 VKK295 pKa = 10.58 CRR297 pKa = 11.84 PYY299 pKa = 9.94 ICYY302 pKa = 8.37 PAHH305 pKa = 6.61 CALLFSVLCQRR316 pKa = 11.84 FQEE319 pKa = 4.19 KK320 pKa = 11.07 LEE322 pKa = 4.1 IFTQNFEE329 pKa = 4.01 SSNAYY334 pKa = 9.93 GFSSANGGLKK344 pKa = 10.37 KK345 pKa = 10.35 LVEE348 pKa = 4.33 WMISTPRR355 pKa = 11.84 GTSRR359 pKa = 11.84 YY360 pKa = 8.91 ICYY363 pKa = 10.56 GDD365 pKa = 3.49 DD366 pKa = 3.32 TRR368 pKa = 11.84 IVVADD373 pKa = 4.19 RR374 pKa = 11.84 NGDD377 pKa = 3.3 RR378 pKa = 11.84 FMVNPDD384 pKa = 3.72 FQQMDD389 pKa = 3.96 GSVDD393 pKa = 3.29 AQTIAWTIDD402 pKa = 3.5 FIIKK406 pKa = 10.27 KK407 pKa = 10.32 LEE409 pKa = 4.15 EE410 pKa = 3.97 EE411 pKa = 4.63 DD412 pKa = 5.01 GEE414 pKa = 4.69 EE415 pKa = 3.92 NCFWRR420 pKa = 11.84 AVAKK424 pKa = 9.94 LWKK427 pKa = 10.52 LMATNPQFVVNGTRR441 pKa = 11.84 VWKK444 pKa = 10.43 KK445 pKa = 10.14 KK446 pKa = 10.65 SSDD449 pKa = 3.18 GLMTGVPGTTLFDD462 pKa = 3.52 TVKK465 pKa = 10.95 AIVSWNFCLEE475 pKa = 3.53 ASARR479 pKa = 11.84 GEE481 pKa = 4.21 VNLLNSQDD489 pKa = 3.31 VTQWMRR495 pKa = 11.84 DD496 pKa = 3.29 QCGLVIKK503 pKa = 10.12 PGTYY507 pKa = 10.18 NPEE510 pKa = 4.12 KK511 pKa = 10.79 LPGEE515 pKa = 3.98 IPNRR519 pKa = 11.84 GEE521 pKa = 3.76 LCGKK525 pKa = 10.33 GKK527 pKa = 10.07 FLGMQIMVEE536 pKa = 4.1 EE537 pKa = 4.55 FEE539 pKa = 4.63 GEE541 pKa = 4.55 TVFVPTLPEE550 pKa = 3.65 EE551 pKa = 4.37 EE552 pKa = 4.42 MIEE555 pKa = 3.97 MLVVQKK561 pKa = 10.79 DD562 pKa = 3.51 DD563 pKa = 4.3 PYY565 pKa = 11.42 QKK567 pKa = 9.3 TRR569 pKa = 11.84 SLTANQRR576 pKa = 11.84 LLFDD580 pKa = 4.01 RR581 pKa = 11.84 MRR583 pKa = 11.84 GLMITFGFTNPLVVSTIHH601 pKa = 6.56 NIVNTIPPTAVLMEE615 pKa = 4.1 TQIKK619 pKa = 9.84 GGSSPEE625 pKa = 4.51 HH626 pKa = 6.21 ITLQDD631 pKa = 3.28 FGYY634 pKa = 9.25 PDD636 pKa = 3.68 SDD638 pKa = 3.38 GFPSVEE644 pKa = 3.95 FCKK647 pKa = 10.45 AVYY650 pKa = 9.98 AGCLEE655 pKa = 4.3 RR656 pKa = 11.84 DD657 pKa = 4.12 RR658 pKa = 11.84 YY659 pKa = 6.72 WTPLFPGLAEE669 pKa = 4.01 VFKK672 pKa = 10.5 EE673 pKa = 3.56 LRR675 pKa = 11.84 EE676 pKa = 4.09 NGRR679 pKa = 11.84 HH680 pKa = 4.53 YY681 pKa = 10.79 LRR683 pKa = 11.84 SVRR686 pKa = 11.84 LVTRR690 pKa = 11.84 EE691 pKa = 3.81 GVEE694 pKa = 3.77 QTVVRR699 pKa = 11.84 EE700 pKa = 4.05 DD701 pKa = 4.14 KK702 pKa = 11.07 IMEE705 pKa = 4.41 TNVLPVALEE714 pKa = 4.06 EE715 pKa = 4.28 PRR717 pKa = 11.84 VPTNLPDD724 pKa = 3.84 FNEE727 pKa = 4.11 RR728 pKa = 11.84 SHH730 pKa = 8.4 IVDD733 pKa = 4.68 LRR735 pKa = 11.84 GATQKK740 pKa = 10.59 FLPDD744 pKa = 3.93 LGQSIRR750 pKa = 11.84 RR751 pKa = 11.84 LLDD754 pKa = 3.41 EE755 pKa = 5.1 YY756 pKa = 10.73 GGSMYY761 pKa = 10.76 VGEE764 pKa = 3.85 ARR766 pKa = 11.84 MRR768 pKa = 11.84 FGCSSNALMRR778 pKa = 11.84 AASKK782 pKa = 10.71 YY783 pKa = 10.16 GFWIDD788 pKa = 3.49 SLYY791 pKa = 11.13 DD792 pKa = 3.56 DD793 pKa = 5.92 GIISKK798 pKa = 9.9 FPIQTPIPTIQDD810 pKa = 3.77 HH811 pKa = 6.29 IRR813 pKa = 11.84 INMEE817 pKa = 3.79 EE818 pKa = 4.17 NKK820 pKa = 10.54 SVVDD824 pKa = 3.57 KK825 pKa = 10.51 GTEE828 pKa = 3.77 QRR830 pKa = 11.84 LKK832 pKa = 10.72 AIEE835 pKa = 3.92 QLDD838 pKa = 3.58 VSDD841 pKa = 3.94 RR842 pKa = 11.84 VVPAVAVLTNPEE854 pKa = 4.15 EE855 pKa = 4.32 MYY857 pKa = 11.13 LDD859 pKa = 3.91 AGFMAGLNRR868 pKa = 11.84 AAISWRR874 pKa = 11.84 GDD876 pKa = 3.26 TDD878 pKa = 3.75 PLQVLGQFLARR889 pKa = 11.84 QPSHH893 pKa = 6.91 FQVKK897 pKa = 8.45 WSSKK901 pKa = 9.05 VVTQTPDD908 pKa = 3.3 RR909 pKa = 11.84 IQAQLKK915 pKa = 10.09 VISQTFDD922 pKa = 2.59 GSRR925 pKa = 11.84 LVGSVIGPNFRR936 pKa = 11.84 MAKK939 pKa = 8.83 VYY941 pKa = 10.5 LVIHH945 pKa = 6.63 IMNVFDD951 pKa = 5.24 SMLVQKK957 pKa = 10.9 GEE959 pKa = 4.17 SEE961 pKa = 3.98 IFRR964 pKa = 11.84 DD965 pKa = 3.73 YY966 pKa = 11.44 LDD968 pKa = 3.26 QRR970 pKa = 11.84 KK971 pKa = 10.14 SIFSPRR977 pKa = 11.84 KK978 pKa = 8.63 PLPPLNPGLEE988 pKa = 3.86 PWNWVEE994 pKa = 4.57 EE995 pKa = 4.4 IEE997 pKa = 4.79 NTIPPQWEE1005 pKa = 3.99 VLQQTRR1011 pKa = 11.84 EE1012 pKa = 4.06 DD1013 pKa = 3.61 PVLRR1017 pKa = 11.84 EE1018 pKa = 3.53 IVDD1021 pKa = 3.92 FVNQRR1026 pKa = 11.84 FAFATEE1032 pKa = 4.01 SQKK1035 pKa = 10.45 VFLYY1039 pKa = 10.89 NRR1041 pKa = 11.84 TNEE1044 pKa = 3.89 LTLVGFTKK1052 pKa = 10.34 KK1053 pKa = 10.35 PLMIQMLVKK1062 pKa = 10.31 EE1063 pKa = 4.88 LYY1065 pKa = 10.74 ALLKK1069 pKa = 10.23 EE1070 pKa = 4.55 FKK1072 pKa = 10.55 KK1073 pKa = 10.61 RR1074 pKa = 11.84 DD1075 pKa = 3.52 ATEE1078 pKa = 3.58 LRR1080 pKa = 11.84 RR1081 pKa = 11.84 MYY1083 pKa = 10.08 EE1084 pKa = 3.74 QTGDD1088 pKa = 3.68 SSVSDD1093 pKa = 3.31 QTIYY1097 pKa = 10.58 PPPAAFADD1105 pKa = 3.91 AEE1107 pKa = 4.43 EE1108 pKa = 4.51 EE1109 pKa = 4.64 VPVSQQQVSQVVEE1122 pKa = 4.44 VYY1124 pKa = 10.55 RR1125 pKa = 11.84 DD1126 pKa = 3.55 AQSAIGDD1133 pKa = 3.8 KK1134 pKa = 10.58 LRR1136 pKa = 11.84 PEE1138 pKa = 4.14 TLVVDD1143 pKa = 4.63 RR1144 pKa = 11.84 EE1145 pKa = 4.49 TSSSAAASRR1154 pKa = 11.84 AVGEE1158 pKa = 4.34 DD1159 pKa = 3.48 VNKK1162 pKa = 10.28 KK1163 pKa = 6.75 SQRR1166 pKa = 11.84 NRR1168 pKa = 11.84 RR1169 pKa = 11.84 THH1171 pKa = 5.27 EE1172 pKa = 3.69 RR1173 pKa = 11.84 EE1174 pKa = 3.5 KK1175 pKa = 10.66 RR1176 pKa = 11.84 QLRR1179 pKa = 11.84 EE1180 pKa = 3.72 ARR1182 pKa = 11.84 QLRR1185 pKa = 11.84 SQRR1188 pKa = 11.84 VRR1190 pKa = 11.84 DD1191 pKa = 3.59 TEE1193 pKa = 3.98 QIVRR1197 pKa = 11.84 NEE1199 pKa = 3.96 PSTGRR1204 pKa = 11.84 ANRR1207 pKa = 11.84 SRR1209 pKa = 11.84 KK1210 pKa = 9.98 NMTQEE1215 pKa = 3.47 MRR1217 pKa = 11.84 TKK1219 pKa = 10.78 LNRR1222 pKa = 11.84 KK1223 pKa = 7.1 VLEE1226 pKa = 3.72 RR1227 pKa = 11.84 RR1228 pKa = 11.84 KK1229 pKa = 9.59 RR1230 pKa = 11.84 QRR1232 pKa = 11.84 KK1233 pKa = 8.26 EE1234 pKa = 3.52 LTEE1237 pKa = 4.36 SKK1239 pKa = 10.84 SSS1241 pKa = 3.28
Molecular weight: 141.46 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.908
IPC2_protein 7.424
IPC_protein 7.249
Toseland 6.927
ProMoST 7.849
Dawson 7.995
Bjellqvist 8.346
Wikipedia 7.907
Rodwell 8.024
Grimsley 6.971
Solomon 8.039
Lehninger 8.053
Nozaki 8.565
DTASelect 8.097
Thurlkill 8.17
EMBOSS 8.214
Sillero 8.478
Patrickios 4.724
IPC_peptide 8.024
IPC2_peptide 7.22
IPC2.peptide.svr19 7.484
Protein with the highest isoelectric point:
>tr|A0A1L3KHW2|A0A1L3KHW2_9VIRU Putative capsid protein OS=Hubei permutotetra-like virus 6 OX=1923080 PE=4 SV=1
MM1 pKa = 7.51 NEE3 pKa = 3.18 TMAGVNRR10 pKa = 11.84 DD11 pKa = 3.46 KK12 pKa = 11.41 NSNSSSKK19 pKa = 11.19 GNGVKK24 pKa = 10.41 KK25 pKa = 10.11 PLTPQQVANRR35 pKa = 11.84 KK36 pKa = 8.86 AKK38 pKa = 8.93 NARR41 pKa = 11.84 RR42 pKa = 11.84 RR43 pKa = 11.84 EE44 pKa = 3.78 RR45 pKa = 11.84 RR46 pKa = 11.84 KK47 pKa = 10.51 NKK49 pKa = 9.71 LAEE52 pKa = 4.14 SQVMGLTPMMFRR64 pKa = 11.84 GGEE67 pKa = 3.96 AGLNPADD74 pKa = 4.11 PRR76 pKa = 11.84 SEE78 pKa = 4.02 TSVLQMGSTPSGRR91 pKa = 11.84 TAALKK96 pKa = 10.11 VLHH99 pKa = 7.01 PNMEE103 pKa = 4.28 ACQMVVKK110 pKa = 10.36 FPDD113 pKa = 4.01 GAVSTSVSMEE123 pKa = 3.57 RR124 pKa = 11.84 RR125 pKa = 11.84 DD126 pKa = 3.7 EE127 pKa = 4.32 FEE129 pKa = 4.66 IPPFPPTVSTDD140 pKa = 2.64 KK141 pKa = 9.98 WDD143 pKa = 4.74 CIVMHH148 pKa = 6.79 LPFLVGRR155 pKa = 11.84 QGVIKK160 pKa = 10.06 WNSTLTTPSEE170 pKa = 3.97 ATLQRR175 pKa = 11.84 VVQVLLEE182 pKa = 4.59 NIGSPDD188 pKa = 3.02 RR189 pKa = 11.84 QYY191 pKa = 11.32 PLWYY195 pKa = 9.8 AYY197 pKa = 8.78 TADD200 pKa = 5.47 GIPFLISILSSSVLTSGVAYY220 pKa = 10.47 SGVSGLSLYY229 pKa = 10.68 AKK231 pKa = 9.99 SIRR234 pKa = 11.84 RR235 pKa = 11.84 TCLGVTTDD243 pKa = 4.99 LDD245 pKa = 4.8 ASDD248 pKa = 5.07 LYY250 pKa = 11.67 NNGRR254 pKa = 11.84 VVSGQWTPDD263 pKa = 2.99 VTTARR268 pKa = 11.84 HH269 pKa = 5.71 DD270 pKa = 3.51 TTDD273 pKa = 2.87 IVTGFVMHH281 pKa = 6.93 TFDD284 pKa = 4.14 IYY286 pKa = 11.26 QMQVPAVTTSSIVSSDD302 pKa = 3.11 EE303 pKa = 4.0 FSRR306 pKa = 11.84 QAEE309 pKa = 4.39 AKK311 pKa = 8.28 TGSYY315 pKa = 9.31 MPLRR319 pKa = 11.84 TCSSTINMTPVQQWRR334 pKa = 11.84 NLAIEE339 pKa = 4.43 LPGVDD344 pKa = 4.39 ADD346 pKa = 4.79 TITSSNCNDD355 pKa = 4.07 LFLQGWSIGVEE366 pKa = 4.18 YY367 pKa = 10.08 WSGLSNQANLRR378 pKa = 11.84 MKK380 pKa = 10.32 VRR382 pKa = 11.84 EE383 pKa = 4.13 DD384 pKa = 3.63 LEE386 pKa = 4.54 IVPSPDD392 pKa = 2.97 SPYY395 pKa = 11.31 SPFATPAYY403 pKa = 8.76 PDD405 pKa = 3.68 DD406 pKa = 3.53 VRR408 pKa = 11.84 ARR410 pKa = 11.84 AVIQEE415 pKa = 4.22 FSRR418 pKa = 11.84 KK419 pKa = 8.52 QPHH422 pKa = 7.34 AYY424 pKa = 7.56 PASYY428 pKa = 11.0 NEE430 pKa = 3.91 NNILLKK436 pKa = 10.81 KK437 pKa = 10.14 LISAVGEE444 pKa = 4.22 VVEE447 pKa = 5.63 DD448 pKa = 3.65 LGLPILSPLVKK459 pKa = 10.18 GVRR462 pKa = 11.84 NFLDD466 pKa = 3.5 SKK468 pKa = 10.43 LWSHH472 pKa = 7.32 ISNLFF477 pKa = 3.33
Molecular weight: 52.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.918
IPC2_protein 7.278
IPC_protein 7.307
Toseland 7.029
ProMoST 7.702
Dawson 7.951
Bjellqvist 8.156
Wikipedia 7.892
Rodwell 7.951
Grimsley 7.088
Solomon 8.068
Lehninger 8.083
Nozaki 8.346
DTASelect 8.039
Thurlkill 8.097
EMBOSS 8.185
Sillero 8.375
Patrickios 4.482
IPC_peptide 8.053
IPC2_peptide 7.293
IPC2.peptide.svr19 7.463
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1718
477
1241
859.0
97.0
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.471 ± 0.51
1.222 ± 0.086
5.471 ± 0.114
6.752 ± 1.166
3.783 ± 0.421
5.879 ± 0.1
1.048 ± 0.104
4.889 ± 0.242
5.413 ± 0.606
8.44 ± 0.027
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.26 ± 0.057
4.54 ± 0.348
6.112 ± 0.296
4.773 ± 0.392
7.276 ± 0.698
7.334 ± 1.563
6.17 ± 0.475
8.324 ± 0.135
1.455 ± 0.11
2.386 ± 0.168
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here