Hubei permutotetra-like virus 6

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1L3KHW2|A0A1L3KHW2_9VIRU Putative capsid protein OS=Hubei permutotetra-like virus 6 OX=1923080 PE=4 SV=1
MM1 pKa = 7.59DD2 pKa = 4.07TSNPVVSSDD11 pKa = 2.99RR12 pKa = 11.84RR13 pKa = 11.84SLRR16 pKa = 11.84EE17 pKa = 3.85AKK19 pKa = 10.01EE20 pKa = 3.46LAVVKK25 pKa = 10.31KK26 pKa = 10.43RR27 pKa = 11.84QDD29 pKa = 2.95LEE31 pKa = 4.4YY32 pKa = 10.58IMNVAQTSTRR42 pKa = 11.84VILPVGEE49 pKa = 4.21RR50 pKa = 11.84ALPEE54 pKa = 4.34AEE56 pKa = 4.2VKK58 pKa = 10.74SIILNLKK65 pKa = 10.09DD66 pKa = 3.51KK67 pKa = 10.35EE68 pKa = 4.41PTVRR72 pKa = 11.84TDD74 pKa = 3.33SEE76 pKa = 4.49DD77 pKa = 3.52MLDD80 pKa = 3.33EE81 pKa = 4.44TVFQLVAPSMPVCGLLSQDD100 pKa = 4.22GVPLHH105 pKa = 6.73PPPIHH110 pKa = 5.75NAKK113 pKa = 10.25GRR115 pKa = 11.84VTTNRR120 pKa = 11.84RR121 pKa = 11.84FGGGAYY127 pKa = 9.14KK128 pKa = 10.51PLGIRR133 pKa = 11.84PMVEE137 pKa = 3.1ILGVRR142 pKa = 11.84TQEE145 pKa = 3.42ITKK148 pKa = 9.99VYY150 pKa = 8.91THH152 pKa = 6.38GTWQGFSNRR161 pKa = 11.84LAKK164 pKa = 10.46QMGRR168 pKa = 11.84KK169 pKa = 8.06CVSMGMTIRR178 pKa = 11.84NSMPGVQMDD187 pKa = 3.58KK188 pKa = 9.47NTPRR192 pKa = 11.84RR193 pKa = 11.84IFFDD197 pKa = 3.06ILNKK201 pKa = 10.16KK202 pKa = 8.63MPARR206 pKa = 11.84KK207 pKa = 8.83PSGWPSLSDD216 pKa = 3.79PLDD219 pKa = 4.13TILDD223 pKa = 4.16NIKK226 pKa = 8.76ITLDD230 pKa = 3.26ASAGPPYY237 pKa = 9.5WRR239 pKa = 11.84NKK241 pKa = 8.22VEE243 pKa = 5.39CMDD246 pKa = 4.08QILSEE251 pKa = 4.66GIPLLVKK258 pKa = 10.25ALKK261 pKa = 10.19EE262 pKa = 3.87NRR264 pKa = 11.84LEE266 pKa = 3.87QFMRR270 pKa = 11.84EE271 pKa = 3.8EE272 pKa = 4.59PEE274 pKa = 4.24FFLCEE279 pKa = 4.11VKK281 pKa = 10.95NKK283 pKa = 9.89LDD285 pKa = 3.5RR286 pKa = 11.84YY287 pKa = 10.37GVDD290 pKa = 3.51EE291 pKa = 5.75LDD293 pKa = 3.92VKK295 pKa = 10.58CRR297 pKa = 11.84PYY299 pKa = 9.94ICYY302 pKa = 8.37PAHH305 pKa = 6.61CALLFSVLCQRR316 pKa = 11.84FQEE319 pKa = 4.19KK320 pKa = 11.07LEE322 pKa = 4.1IFTQNFEE329 pKa = 4.01SSNAYY334 pKa = 9.93GFSSANGGLKK344 pKa = 10.37KK345 pKa = 10.35LVEE348 pKa = 4.33WMISTPRR355 pKa = 11.84GTSRR359 pKa = 11.84YY360 pKa = 8.91ICYY363 pKa = 10.56GDD365 pKa = 3.49DD366 pKa = 3.32TRR368 pKa = 11.84IVVADD373 pKa = 4.19RR374 pKa = 11.84NGDD377 pKa = 3.3RR378 pKa = 11.84FMVNPDD384 pKa = 3.72FQQMDD389 pKa = 3.96GSVDD393 pKa = 3.29AQTIAWTIDD402 pKa = 3.5FIIKK406 pKa = 10.27KK407 pKa = 10.32LEE409 pKa = 4.15EE410 pKa = 3.97EE411 pKa = 4.63DD412 pKa = 5.01GEE414 pKa = 4.69EE415 pKa = 3.92NCFWRR420 pKa = 11.84AVAKK424 pKa = 9.94LWKK427 pKa = 10.52LMATNPQFVVNGTRR441 pKa = 11.84VWKK444 pKa = 10.43KK445 pKa = 10.14KK446 pKa = 10.65SSDD449 pKa = 3.18GLMTGVPGTTLFDD462 pKa = 3.52TVKK465 pKa = 10.95AIVSWNFCLEE475 pKa = 3.53ASARR479 pKa = 11.84GEE481 pKa = 4.21VNLLNSQDD489 pKa = 3.31VTQWMRR495 pKa = 11.84DD496 pKa = 3.29QCGLVIKK503 pKa = 10.12PGTYY507 pKa = 10.18NPEE510 pKa = 4.12KK511 pKa = 10.79LPGEE515 pKa = 3.98IPNRR519 pKa = 11.84GEE521 pKa = 3.76LCGKK525 pKa = 10.33GKK527 pKa = 10.07FLGMQIMVEE536 pKa = 4.1EE537 pKa = 4.55FEE539 pKa = 4.63GEE541 pKa = 4.55TVFVPTLPEE550 pKa = 3.65EE551 pKa = 4.37EE552 pKa = 4.42MIEE555 pKa = 3.97MLVVQKK561 pKa = 10.79DD562 pKa = 3.51DD563 pKa = 4.3PYY565 pKa = 11.42QKK567 pKa = 9.3TRR569 pKa = 11.84SLTANQRR576 pKa = 11.84LLFDD580 pKa = 4.01RR581 pKa = 11.84MRR583 pKa = 11.84GLMITFGFTNPLVVSTIHH601 pKa = 6.56NIVNTIPPTAVLMEE615 pKa = 4.1TQIKK619 pKa = 9.84GGSSPEE625 pKa = 4.51HH626 pKa = 6.21ITLQDD631 pKa = 3.28FGYY634 pKa = 9.25PDD636 pKa = 3.68SDD638 pKa = 3.38GFPSVEE644 pKa = 3.95FCKK647 pKa = 10.45AVYY650 pKa = 9.98AGCLEE655 pKa = 4.3RR656 pKa = 11.84DD657 pKa = 4.12RR658 pKa = 11.84YY659 pKa = 6.72WTPLFPGLAEE669 pKa = 4.01VFKK672 pKa = 10.5EE673 pKa = 3.56LRR675 pKa = 11.84EE676 pKa = 4.09NGRR679 pKa = 11.84HH680 pKa = 4.53YY681 pKa = 10.79LRR683 pKa = 11.84SVRR686 pKa = 11.84LVTRR690 pKa = 11.84EE691 pKa = 3.81GVEE694 pKa = 3.77QTVVRR699 pKa = 11.84EE700 pKa = 4.05DD701 pKa = 4.14KK702 pKa = 11.07IMEE705 pKa = 4.41TNVLPVALEE714 pKa = 4.06EE715 pKa = 4.28PRR717 pKa = 11.84VPTNLPDD724 pKa = 3.84FNEE727 pKa = 4.11RR728 pKa = 11.84SHH730 pKa = 8.4IVDD733 pKa = 4.68LRR735 pKa = 11.84GATQKK740 pKa = 10.59FLPDD744 pKa = 3.93LGQSIRR750 pKa = 11.84RR751 pKa = 11.84LLDD754 pKa = 3.41EE755 pKa = 5.1YY756 pKa = 10.73GGSMYY761 pKa = 10.76VGEE764 pKa = 3.85ARR766 pKa = 11.84MRR768 pKa = 11.84FGCSSNALMRR778 pKa = 11.84AASKK782 pKa = 10.71YY783 pKa = 10.16GFWIDD788 pKa = 3.49SLYY791 pKa = 11.13DD792 pKa = 3.56DD793 pKa = 5.92GIISKK798 pKa = 9.9FPIQTPIPTIQDD810 pKa = 3.77HH811 pKa = 6.29IRR813 pKa = 11.84INMEE817 pKa = 3.79EE818 pKa = 4.17NKK820 pKa = 10.54SVVDD824 pKa = 3.57KK825 pKa = 10.51GTEE828 pKa = 3.77QRR830 pKa = 11.84LKK832 pKa = 10.72AIEE835 pKa = 3.92QLDD838 pKa = 3.58VSDD841 pKa = 3.94RR842 pKa = 11.84VVPAVAVLTNPEE854 pKa = 4.15EE855 pKa = 4.32MYY857 pKa = 11.13LDD859 pKa = 3.91AGFMAGLNRR868 pKa = 11.84AAISWRR874 pKa = 11.84GDD876 pKa = 3.26TDD878 pKa = 3.75PLQVLGQFLARR889 pKa = 11.84QPSHH893 pKa = 6.91FQVKK897 pKa = 8.45WSSKK901 pKa = 9.05VVTQTPDD908 pKa = 3.3RR909 pKa = 11.84IQAQLKK915 pKa = 10.09VISQTFDD922 pKa = 2.59GSRR925 pKa = 11.84LVGSVIGPNFRR936 pKa = 11.84MAKK939 pKa = 8.83VYY941 pKa = 10.5LVIHH945 pKa = 6.63IMNVFDD951 pKa = 5.24SMLVQKK957 pKa = 10.9GEE959 pKa = 4.17SEE961 pKa = 3.98IFRR964 pKa = 11.84DD965 pKa = 3.73YY966 pKa = 11.44LDD968 pKa = 3.26QRR970 pKa = 11.84KK971 pKa = 10.14SIFSPRR977 pKa = 11.84KK978 pKa = 8.63PLPPLNPGLEE988 pKa = 3.86PWNWVEE994 pKa = 4.57EE995 pKa = 4.4IEE997 pKa = 4.79NTIPPQWEE1005 pKa = 3.99VLQQTRR1011 pKa = 11.84EE1012 pKa = 4.06DD1013 pKa = 3.61PVLRR1017 pKa = 11.84EE1018 pKa = 3.53IVDD1021 pKa = 3.92FVNQRR1026 pKa = 11.84FAFATEE1032 pKa = 4.01SQKK1035 pKa = 10.45VFLYY1039 pKa = 10.89NRR1041 pKa = 11.84TNEE1044 pKa = 3.89LTLVGFTKK1052 pKa = 10.34KK1053 pKa = 10.35PLMIQMLVKK1062 pKa = 10.31EE1063 pKa = 4.88LYY1065 pKa = 10.74ALLKK1069 pKa = 10.23EE1070 pKa = 4.55FKK1072 pKa = 10.55KK1073 pKa = 10.61RR1074 pKa = 11.84DD1075 pKa = 3.52ATEE1078 pKa = 3.58LRR1080 pKa = 11.84RR1081 pKa = 11.84MYY1083 pKa = 10.08EE1084 pKa = 3.74QTGDD1088 pKa = 3.68SSVSDD1093 pKa = 3.31QTIYY1097 pKa = 10.58PPPAAFADD1105 pKa = 3.91AEE1107 pKa = 4.43EE1108 pKa = 4.51EE1109 pKa = 4.64VPVSQQQVSQVVEE1122 pKa = 4.44VYY1124 pKa = 10.55RR1125 pKa = 11.84DD1126 pKa = 3.55AQSAIGDD1133 pKa = 3.8KK1134 pKa = 10.58LRR1136 pKa = 11.84PEE1138 pKa = 4.14TLVVDD1143 pKa = 4.63RR1144 pKa = 11.84EE1145 pKa = 4.49TSSSAAASRR1154 pKa = 11.84AVGEE1158 pKa = 4.34DD1159 pKa = 3.48VNKK1162 pKa = 10.28KK1163 pKa = 6.75SQRR1166 pKa = 11.84NRR1168 pKa = 11.84RR1169 pKa = 11.84THH1171 pKa = 5.27EE1172 pKa = 3.69RR1173 pKa = 11.84EE1174 pKa = 3.5KK1175 pKa = 10.66RR1176 pKa = 11.84QLRR1179 pKa = 11.84EE1180 pKa = 3.72ARR1182 pKa = 11.84QLRR1185 pKa = 11.84SQRR1188 pKa = 11.84VRR1190 pKa = 11.84DD1191 pKa = 3.59TEE1193 pKa = 3.98QIVRR1197 pKa = 11.84NEE1199 pKa = 3.96PSTGRR1204 pKa = 11.84ANRR1207 pKa = 11.84SRR1209 pKa = 11.84KK1210 pKa = 9.98NMTQEE1215 pKa = 3.47MRR1217 pKa = 11.84TKK1219 pKa = 10.78LNRR1222 pKa = 11.84KK1223 pKa = 7.1VLEE1226 pKa = 3.72RR1227 pKa = 11.84RR1228 pKa = 11.84KK1229 pKa = 9.59RR1230 pKa = 11.84QRR1232 pKa = 11.84KK1233 pKa = 8.26EE1234 pKa = 3.52LTEE1237 pKa = 4.36SKK1239 pKa = 10.84SSS1241 pKa = 3.28

Molecular weight:
141.46 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1L3KHW2|A0A1L3KHW2_9VIRU Putative capsid protein OS=Hubei permutotetra-like virus 6 OX=1923080 PE=4 SV=1
MM1 pKa = 7.51NEE3 pKa = 3.18TMAGVNRR10 pKa = 11.84DD11 pKa = 3.46KK12 pKa = 11.41NSNSSSKK19 pKa = 11.19GNGVKK24 pKa = 10.41KK25 pKa = 10.11PLTPQQVANRR35 pKa = 11.84KK36 pKa = 8.86AKK38 pKa = 8.93NARR41 pKa = 11.84RR42 pKa = 11.84RR43 pKa = 11.84EE44 pKa = 3.78RR45 pKa = 11.84RR46 pKa = 11.84KK47 pKa = 10.51NKK49 pKa = 9.71LAEE52 pKa = 4.14SQVMGLTPMMFRR64 pKa = 11.84GGEE67 pKa = 3.96AGLNPADD74 pKa = 4.11PRR76 pKa = 11.84SEE78 pKa = 4.02TSVLQMGSTPSGRR91 pKa = 11.84TAALKK96 pKa = 10.11VLHH99 pKa = 7.01PNMEE103 pKa = 4.28ACQMVVKK110 pKa = 10.36FPDD113 pKa = 4.01GAVSTSVSMEE123 pKa = 3.57RR124 pKa = 11.84RR125 pKa = 11.84DD126 pKa = 3.7EE127 pKa = 4.32FEE129 pKa = 4.66IPPFPPTVSTDD140 pKa = 2.64KK141 pKa = 9.98WDD143 pKa = 4.74CIVMHH148 pKa = 6.79LPFLVGRR155 pKa = 11.84QGVIKK160 pKa = 10.06WNSTLTTPSEE170 pKa = 3.97ATLQRR175 pKa = 11.84VVQVLLEE182 pKa = 4.59NIGSPDD188 pKa = 3.02RR189 pKa = 11.84QYY191 pKa = 11.32PLWYY195 pKa = 9.8AYY197 pKa = 8.78TADD200 pKa = 5.47GIPFLISILSSSVLTSGVAYY220 pKa = 10.47SGVSGLSLYY229 pKa = 10.68AKK231 pKa = 9.99SIRR234 pKa = 11.84RR235 pKa = 11.84TCLGVTTDD243 pKa = 4.99LDD245 pKa = 4.8ASDD248 pKa = 5.07LYY250 pKa = 11.67NNGRR254 pKa = 11.84VVSGQWTPDD263 pKa = 2.99VTTARR268 pKa = 11.84HH269 pKa = 5.71DD270 pKa = 3.51TTDD273 pKa = 2.87IVTGFVMHH281 pKa = 6.93TFDD284 pKa = 4.14IYY286 pKa = 11.26QMQVPAVTTSSIVSSDD302 pKa = 3.11EE303 pKa = 4.0FSRR306 pKa = 11.84QAEE309 pKa = 4.39AKK311 pKa = 8.28TGSYY315 pKa = 9.31MPLRR319 pKa = 11.84TCSSTINMTPVQQWRR334 pKa = 11.84NLAIEE339 pKa = 4.43LPGVDD344 pKa = 4.39ADD346 pKa = 4.79TITSSNCNDD355 pKa = 4.07LFLQGWSIGVEE366 pKa = 4.18YY367 pKa = 10.08WSGLSNQANLRR378 pKa = 11.84MKK380 pKa = 10.32VRR382 pKa = 11.84EE383 pKa = 4.13DD384 pKa = 3.63LEE386 pKa = 4.54IVPSPDD392 pKa = 2.97SPYY395 pKa = 11.31SPFATPAYY403 pKa = 8.76PDD405 pKa = 3.68DD406 pKa = 3.53VRR408 pKa = 11.84ARR410 pKa = 11.84AVIQEE415 pKa = 4.22FSRR418 pKa = 11.84KK419 pKa = 8.52QPHH422 pKa = 7.34AYY424 pKa = 7.56PASYY428 pKa = 11.0NEE430 pKa = 3.91NNILLKK436 pKa = 10.81KK437 pKa = 10.14LISAVGEE444 pKa = 4.22VVEE447 pKa = 5.63DD448 pKa = 3.65LGLPILSPLVKK459 pKa = 10.18GVRR462 pKa = 11.84NFLDD466 pKa = 3.5SKK468 pKa = 10.43LWSHH472 pKa = 7.32ISNLFF477 pKa = 3.33

Molecular weight:
52.53 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1718

477

1241

859.0

97.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.471 ± 0.51

1.222 ± 0.086

5.471 ± 0.114

6.752 ± 1.166

3.783 ± 0.421

5.879 ± 0.1

1.048 ± 0.104

4.889 ± 0.242

5.413 ± 0.606

8.44 ± 0.027

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.26 ± 0.057

4.54 ± 0.348

6.112 ± 0.296

4.773 ± 0.392

7.276 ± 0.698

7.334 ± 1.563

6.17 ± 0.475

8.324 ± 0.135

1.455 ± 0.11

2.386 ± 0.168

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski