Lysinibacillus composti
Average proteome isoelectric point is 6.13
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3831 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3N9UI56|A0A3N9UI56_9BACI Ferritin-like domain-containing protein OS=Lysinibacillus composti OX=720633 GN=EBB45_03745 PE=4 SV=1
MM1 pKa = 7.45 NDD3 pKa = 2.88 KK4 pKa = 9.74 TFLLNMYY11 pKa = 11.12 AMLNQMQSQLNQCKK25 pKa = 10.24 SYY27 pKa = 11.35 LEE29 pKa = 4.34 NEE31 pKa = 4.19 LDD33 pKa = 3.75 EE34 pKa = 5.31 KK35 pKa = 11.25 GYY37 pKa = 10.85 DD38 pKa = 3.62 YY39 pKa = 11.21 STMLEE44 pKa = 4.46 DD45 pKa = 3.58 YY46 pKa = 10.54 DD47 pKa = 4.71 VAGFDD52 pKa = 3.29 QDD54 pKa = 3.7 EE55 pKa = 4.69 VFNEE59 pKa = 4.33 RR60 pKa = 11.84 PSS62 pKa = 3.19
Molecular weight: 7.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.759
IPC2_protein 3.859
IPC_protein 3.757
Toseland 3.567
ProMoST 3.935
Dawson 3.745
Bjellqvist 3.91
Wikipedia 3.694
Rodwell 3.592
Grimsley 3.478
Solomon 3.719
Lehninger 3.681
Nozaki 3.884
DTASelect 4.062
Thurlkill 3.63
EMBOSS 3.706
Sillero 3.884
Patrickios 0.693
IPC_peptide 3.719
IPC2_peptide 3.846
IPC2.peptide.svr19 3.779
Protein with the highest isoelectric point:
>tr|A0A3N9UCE0|A0A3N9UCE0_9BACI Histidine kinase OS=Lysinibacillus composti OX=720633 GN=walK PE=4 SV=1
MM1 pKa = 7.36 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 9.54 QPKK8 pKa = 9.44 KK9 pKa = 7.96 RR10 pKa = 11.84 KK11 pKa = 8.69 HH12 pKa = 5.94 SKK14 pKa = 8.54 VHH16 pKa = 5.68 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MSTKK25 pKa = 10.19 NGRR28 pKa = 11.84 KK29 pKa = 8.91 VLAARR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 KK37 pKa = 8.81 GRR39 pKa = 11.84 KK40 pKa = 8.75 VLSAA44 pKa = 4.05
Molecular weight: 5.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.393
IPC2_protein 11.067
IPC_protein 12.398
Toseland 12.559
ProMoST 13.042
Dawson 12.574
Bjellqvist 12.559
Wikipedia 13.027
Rodwell 12.398
Grimsley 12.603
Solomon 13.056
Lehninger 12.954
Nozaki 12.559
DTASelect 12.559
Thurlkill 12.559
EMBOSS 13.056
Sillero 12.559
Patrickios 12.135
IPC_peptide 13.056
IPC2_peptide 12.032
IPC2.peptide.svr19 8.988
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3831
0
3831
1145423
8
1654
299.0
33.54
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.913 ± 0.042
0.69 ± 0.012
4.95 ± 0.031
7.65 ± 0.047
4.622 ± 0.037
6.72 ± 0.045
1.969 ± 0.018
8.31 ± 0.039
6.731 ± 0.036
9.723 ± 0.046
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.637 ± 0.016
4.746 ± 0.032
3.527 ± 0.021
3.741 ± 0.027
3.791 ± 0.028
6.054 ± 0.028
5.586 ± 0.03
7.188 ± 0.03
0.934 ± 0.014
3.518 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here