Capybara microvirus Cap1_SP_145
Average proteome isoelectric point is 6.52
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W7X8|A0A4P8W7X8_9VIRU Uncharacterized protein OS=Capybara microvirus Cap1_SP_145 OX=2585390 PE=4 SV=1
MM1 pKa = 7.83 KK2 pKa = 10.49 YY3 pKa = 10.56 LEE5 pKa = 5.05 RR6 pKa = 11.84 DD7 pKa = 3.17 NKK9 pKa = 10.97 SIVTVTEE16 pKa = 3.85 YY17 pKa = 10.48 MSLAEE22 pKa = 4.46 CIAYY26 pKa = 9.02 YY27 pKa = 10.63 EE28 pKa = 4.47 RR29 pKa = 11.84 TNEE32 pKa = 4.08 LPPIGEE38 pKa = 4.47 TKK40 pKa = 10.81 GEE42 pKa = 4.08 YY43 pKa = 9.98 DD44 pKa = 3.68 DD45 pKa = 6.11 SITISEE51 pKa = 4.31 AEE53 pKa = 3.74 ARR55 pKa = 11.84 TINVAKK61 pKa = 10.38 LYY63 pKa = 10.81 KK64 pKa = 10.21 FDD66 pKa = 4.95 DD67 pKa = 4.24 DD68 pKa = 4.54 RR69 pKa = 11.84 VSDD72 pKa = 3.82 MDD74 pKa = 3.48 ISDD77 pKa = 3.81 MRR79 pKa = 11.84 NSSSEE84 pKa = 4.15 SYY86 pKa = 10.45 ILEE89 pKa = 4.09 ASAEE93 pKa = 4.03 KK94 pKa = 10.5 SAEE97 pKa = 3.8 KK98 pKa = 10.31 SAEE101 pKa = 3.63 ISVEE105 pKa = 4.11 NISVEE110 pKa = 4.2 QVSPQATAEE119 pKa = 4.24 KK120 pKa = 10.68
Molecular weight: 13.48 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.387
IPC2_protein 4.329
IPC_protein 4.228
Toseland 4.075
ProMoST 4.329
Dawson 4.164
Bjellqvist 4.329
Wikipedia 4.037
Rodwell 4.062
Grimsley 3.986
Solomon 4.164
Lehninger 4.113
Nozaki 4.279
DTASelect 4.406
Thurlkill 4.075
EMBOSS 4.05
Sillero 4.329
Patrickios 3.656
IPC_peptide 4.177
IPC2_peptide 4.329
IPC2.peptide.svr19 4.255
Protein with the highest isoelectric point:
>tr|A0A4P8W5G0|A0A4P8W5G0_9VIRU Uncharacterized protein OS=Capybara microvirus Cap1_SP_145 OX=2585390 PE=4 SV=1
MM1 pKa = 7.53 GSGLHH6 pKa = 6.05 RR7 pKa = 11.84 VNTVPCGKK15 pKa = 10.05 CVACRR20 pKa = 11.84 SNKK23 pKa = 9.11 RR24 pKa = 11.84 EE25 pKa = 3.69 EE26 pKa = 4.09 WASRR30 pKa = 11.84 LAMEE34 pKa = 5.34 YY35 pKa = 9.22 YY36 pKa = 7.87 TTFKK40 pKa = 10.78 KK41 pKa = 10.77 GFEE44 pKa = 4.32 TFFITLTFNDD54 pKa = 4.37 EE55 pKa = 4.08 NLQNPSLDD63 pKa = 3.44 KK64 pKa = 10.98 RR65 pKa = 11.84 DD66 pKa = 3.23 IQLFNKK72 pKa = 9.7 KK73 pKa = 8.87 FRR75 pKa = 11.84 HH76 pKa = 5.05 YY77 pKa = 10.94 LSYY80 pKa = 10.95 KK81 pKa = 9.75 YY82 pKa = 8.95 FFCGEE87 pKa = 4.11 YY88 pKa = 10.56 GDD90 pKa = 4.28 KK91 pKa = 10.39 FDD93 pKa = 4.43 RR94 pKa = 11.84 KK95 pKa = 9.63 HH96 pKa = 4.14 YY97 pKa = 10.02 HH98 pKa = 6.86 ALIFCNTVVTPEE110 pKa = 3.92 YY111 pKa = 9.27 FTRR114 pKa = 11.84 SLEE117 pKa = 4.14 KK118 pKa = 9.13 CWPYY122 pKa = 11.54 GFVKK126 pKa = 10.64 ADD128 pKa = 2.94 NFTYY132 pKa = 10.74 ARR134 pKa = 11.84 ARR136 pKa = 11.84 YY137 pKa = 7.08 VAKK140 pKa = 10.73 YY141 pKa = 10.24 ALKK144 pKa = 10.43 DD145 pKa = 4.13 DD146 pKa = 3.92 NWSDD150 pKa = 3.95 TNLSPPFALMSTKK163 pKa = 10.14 PAICYY168 pKa = 10.14 DD169 pKa = 3.87 YY170 pKa = 11.74 VKK172 pKa = 10.57 LFHH175 pKa = 6.12 NQIYY179 pKa = 10.37 DD180 pKa = 3.78 GQSLFYY186 pKa = 9.53 CDD188 pKa = 2.75 SVGYY192 pKa = 9.99 HH193 pKa = 6.36 RR194 pKa = 11.84 LPRR197 pKa = 11.84 YY198 pKa = 9.07 VKK200 pKa = 10.07 EE201 pKa = 4.48 KK202 pKa = 10.11 IFSKK206 pKa = 10.01 IWLRR210 pKa = 11.84 EE211 pKa = 3.67 QNDD214 pKa = 3.75 AYY216 pKa = 11.41 SRR218 pKa = 11.84 LEE220 pKa = 3.96 FNKK223 pKa = 10.08 EE224 pKa = 3.78 LYY226 pKa = 10.15 VLRR229 pKa = 11.84 NLQLLHH235 pKa = 6.7 GGNHH239 pKa = 5.91 NAVNSAYY246 pKa = 10.01 RR247 pKa = 11.84 AYY249 pKa = 9.83 MYY251 pKa = 10.37 QKK253 pKa = 10.41 YY254 pKa = 9.9 KK255 pKa = 10.78 SPEE258 pKa = 3.55 VNYY261 pKa = 10.02 FSNKK265 pKa = 9.25 YY266 pKa = 9.4 IYY268 pKa = 10.75 SRR270 pKa = 11.84 FKK272 pKa = 11.5 NNFF275 pKa = 2.99
Molecular weight: 33.0 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.721
IPC2_protein 8.799
IPC_protein 8.682
Toseland 9.019
ProMoST 9.033
Dawson 9.428
Bjellqvist 9.311
Wikipedia 9.648
Rodwell 9.531
Grimsley 9.516
Solomon 9.458
Lehninger 9.399
Nozaki 9.311
DTASelect 9.224
Thurlkill 9.268
EMBOSS 9.516
Sillero 9.443
Patrickios 3.745
IPC_peptide 9.443
IPC2_peptide 8.141
IPC2.peptide.svr19 7.825
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
1482
106
604
296.4
34.2
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.478 ± 0.56
1.282 ± 0.429
4.858 ± 0.775
6.005 ± 1.09
5.398 ± 0.916
4.858 ± 0.625
1.619 ± 0.554
5.87 ± 0.689
6.883 ± 0.554
9.109 ± 1.038
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.092 ± 0.173
7.152 ± 0.949
3.509 ± 0.411
4.926 ± 1.167
3.914 ± 0.529
8.367 ± 0.683
5.466 ± 0.365
3.914 ± 0.562
1.552 ± 0.224
6.748 ± 0.861
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here