Capybara microvirus Cap1_SP_178
Average proteome isoelectric point is 7.3
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W837|A0A4P8W837_9VIRU Minor capsid protein OS=Capybara microvirus Cap1_SP_178 OX=2585405 PE=4 SV=1
MM1 pKa = 7.71 LSTSPFSSTTQRR13 pKa = 11.84 SLSAMPSFFTRR24 pKa = 11.84 FSPPPNVGFSSSSPSLTLQSDD45 pKa = 4.08 LKK47 pKa = 11.14 DD48 pKa = 3.46 SLVEE52 pKa = 3.96 NVLARR57 pKa = 11.84 HH58 pKa = 5.04 VAGLEE63 pKa = 3.99 RR64 pKa = 11.84 PRR66 pKa = 11.84 TPIFGDD72 pKa = 4.68 FSHH75 pKa = 6.66 TPKK78 pKa = 10.83 DD79 pKa = 3.19 FAEE82 pKa = 4.62 AYY84 pKa = 9.54 EE85 pKa = 4.23 IFSDD89 pKa = 3.45 ASEE92 pKa = 4.61 RR93 pKa = 11.84 FSQLPSEE100 pKa = 4.43 VRR102 pKa = 11.84 DD103 pKa = 3.67 RR104 pKa = 11.84 FANDD108 pKa = 3.57 PLKK111 pKa = 11.04 LLTFLSDD118 pKa = 3.15 PRR120 pKa = 11.84 NRR122 pKa = 11.84 EE123 pKa = 3.27 EE124 pKa = 4.95 AIRR127 pKa = 11.84 LNLVTDD133 pKa = 4.4 RR134 pKa = 11.84 ASGPVEE140 pKa = 3.99 GSTVAVTPLDD150 pKa = 4.24 GIRR153 pKa = 11.84 PGDD156 pKa = 3.66 TNSQSKK162 pKa = 10.59 KK163 pKa = 10.47 DD164 pKa = 3.5
Molecular weight: 18.01 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.384
IPC2_protein 5.385
IPC_protein 5.258
Toseland 5.258
ProMoST 5.207
Dawson 5.283
Bjellqvist 5.423
Wikipedia 5.194
Rodwell 5.194
Grimsley 5.194
Solomon 5.283
Lehninger 5.245
Nozaki 5.423
DTASelect 5.601
Thurlkill 5.27
EMBOSS 5.258
Sillero 5.486
Patrickios 4.851
IPC_peptide 5.296
IPC2_peptide 5.486
IPC2.peptide.svr19 5.581
Protein with the highest isoelectric point:
>tr|A0A4P8W4V4|A0A4P8W4V4_9VIRU Major capsid protein OS=Capybara microvirus Cap1_SP_178 OX=2585405 PE=3 SV=1
MM1 pKa = 7.36 SVFKK5 pKa = 10.89 KK6 pKa = 10.22 VVKK9 pKa = 10.02 SVKK12 pKa = 9.97 KK13 pKa = 10.42 RR14 pKa = 11.84 IEE16 pKa = 3.85 HH17 pKa = 6.62 LKK19 pKa = 10.52 RR20 pKa = 11.84 NPLHH24 pKa = 4.88 YY25 pKa = 10.0 HH26 pKa = 5.89 KK27 pKa = 10.72 KK28 pKa = 10.11 LLRR31 pKa = 11.84 SLSNPHH37 pKa = 6.31 LKK39 pKa = 10.61 KK40 pKa = 10.64 NLGKK44 pKa = 10.16 ILTGQSRR51 pKa = 11.84 HH52 pKa = 5.82 ASPAFSASSVASSGPVYY69 pKa = 10.33 NGKK72 pKa = 10.25 SISEE76 pKa = 4.15 MMM78 pKa = 4.56
Molecular weight: 8.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.213
IPC2_protein 10.233
IPC_protein 10.774
Toseland 11.272
ProMoST 10.877
Dawson 11.316
Bjellqvist 10.979
Wikipedia 11.491
Rodwell 11.842
Grimsley 11.345
Solomon 11.447
Lehninger 11.418
Nozaki 11.228
DTASelect 10.979
Thurlkill 11.242
EMBOSS 11.667
Sillero 11.242
Patrickios 11.579
IPC_peptide 11.462
IPC2_peptide 9.56
IPC2.peptide.svr19 8.138
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
1525
78
552
254.2
28.57
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.148 ± 1.27
1.902 ± 0.854
5.902 ± 0.457
4.131 ± 0.435
5.967 ± 1.275
6.098 ± 0.923
2.492 ± 0.432
5.049 ± 0.491
4.787 ± 0.928
8.721 ± 0.717
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.967 ± 0.308
4.0 ± 0.93
6.557 ± 0.612
3.541 ± 0.637
6.885 ± 0.655
10.098 ± 1.188
4.328 ± 0.522
5.508 ± 0.421
1.311 ± 0.33
3.607 ± 0.804
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here