Cervid alphaherpesvirus 2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Peploviricota; Herviviricetes; Herpesvirales; Herpesviridae; Alphaherpesvirinae; Varicellovirus

Average proteome isoelectric point is 6.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 68 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A455JQ30|A0A455JQ30_9ALPH DNA primase OS=Cervid alphaherpesvirus 2 OX=365327 GN=UL52 PE=3 SV=1
MM1 pKa = 7.77CAPPPPPPPPPSRR14 pKa = 11.84WLPLLLLLWPLLWPPLGAGAEE35 pKa = 4.16PGPGALARR43 pKa = 11.84PVGVIVARR51 pKa = 11.84AGAPVFLPGDD61 pKa = 3.58AAVPRR66 pKa = 11.84PGPAPGPDD74 pKa = 2.86ARR76 pKa = 11.84AVRR79 pKa = 11.84GWSRR83 pKa = 11.84LASACPPPGPATEE96 pKa = 4.1VCIDD100 pKa = 3.44GHH102 pKa = 5.29QCFADD107 pKa = 3.76VVVDD111 pKa = 4.31AACLPTVSLEE121 pKa = 4.01PLAVAEE127 pKa = 4.16LSGGGGAGRR136 pKa = 11.84LADD139 pKa = 3.73LAEE142 pKa = 4.02RR143 pKa = 11.84RR144 pKa = 11.84VFAQLDD150 pKa = 3.85YY151 pKa = 11.59NEE153 pKa = 4.26TGVWIPNATAADD165 pKa = 3.46AGVYY169 pKa = 9.57FLYY172 pKa = 10.54DD173 pKa = 3.22RR174 pKa = 11.84RR175 pKa = 11.84LGEE178 pKa = 4.19AGGEE182 pKa = 4.17EE183 pKa = 4.33THH185 pKa = 6.58SALALRR191 pKa = 11.84VEE193 pKa = 4.29ADD195 pKa = 3.92EE196 pKa = 5.03IEE198 pKa = 4.32GDD200 pKa = 3.76EE201 pKa = 4.6DD202 pKa = 3.58EE203 pKa = 4.38EE204 pKa = 4.25AARR207 pKa = 11.84GRR209 pKa = 11.84STAAPRR215 pKa = 11.84TPAPRR220 pKa = 11.84TPAAPSPAPARR231 pKa = 11.84HH232 pKa = 5.59GARR235 pKa = 11.84FRR237 pKa = 11.84VLPYY241 pKa = 9.52HH242 pKa = 5.24SHH244 pKa = 6.29VYY246 pKa = 9.57TPGDD250 pKa = 3.7SFQLAVRR257 pKa = 11.84LQSEE261 pKa = 4.88FFDD264 pKa = 3.73EE265 pKa = 4.8TPFAAAIDD273 pKa = 4.11WYY275 pKa = 9.92FLRR278 pKa = 11.84PAADD282 pKa = 3.52CALVRR287 pKa = 11.84LYY289 pKa = 7.34EE290 pKa = 4.0TCIFHH295 pKa = 7.43PEE297 pKa = 3.75APACLHH303 pKa = 6.34PVDD306 pKa = 4.85ARR308 pKa = 11.84CSFASPYY315 pKa = 10.14RR316 pKa = 11.84SEE318 pKa = 4.15TVYY321 pKa = 10.85GRR323 pKa = 11.84LYY325 pKa = 9.87PRR327 pKa = 11.84CDD329 pKa = 3.19PAAGARR335 pKa = 11.84WPRR338 pKa = 11.84EE339 pKa = 4.11CEE341 pKa = 3.97GASYY345 pKa = 7.92TAPGAHH351 pKa = 6.72ARR353 pKa = 11.84PAEE356 pKa = 4.24NGADD360 pKa = 3.84LVFADD365 pKa = 5.18APAAAAGLYY374 pKa = 10.31VFVLQYY380 pKa = 10.55NGHH383 pKa = 5.37VEE385 pKa = 3.7AWDD388 pKa = 3.77YY389 pKa = 11.88SLVATSDD396 pKa = 3.38RR397 pKa = 11.84LVRR400 pKa = 11.84AVVDD404 pKa = 3.49HH405 pKa = 6.23TRR407 pKa = 11.84PEE409 pKa = 4.04AAEE412 pKa = 4.55GPGPSAAPGGGEE424 pKa = 4.17PASAPAPARR433 pKa = 11.84RR434 pKa = 11.84FAPWLVALGGVLALVALGGLAALGVWGCAQRR465 pKa = 11.84AARR468 pKa = 11.84RR469 pKa = 11.84RR470 pKa = 11.84TYY472 pKa = 10.78DD473 pKa = 2.73ILNPFGSAYY482 pKa = 9.76TSLPTNDD489 pKa = 3.89PFEE492 pKa = 5.04SSGEE496 pKa = 4.11EE497 pKa = 3.74EE498 pKa = 4.51GGEE501 pKa = 4.28DD502 pKa = 4.65FPPPPGDD509 pKa = 3.43EE510 pKa = 3.88TDD512 pKa = 4.65SFDD515 pKa = 3.89EE516 pKa = 5.38DD517 pKa = 3.92SDD519 pKa = 4.2EE520 pKa = 4.58EE521 pKa = 4.11PSAPYY526 pKa = 10.18EE527 pKa = 4.12LADD530 pKa = 4.63APYY533 pKa = 9.81EE534 pKa = 4.05LADD537 pKa = 4.23AEE539 pKa = 4.43PAGLARR545 pKa = 11.84TPAGGPRR552 pKa = 11.84ASRR555 pKa = 11.84SGFKK559 pKa = 9.97VWFRR563 pKa = 11.84DD564 pKa = 3.65PVEE567 pKa = 4.55EE568 pKa = 4.29EE569 pKa = 4.07PGSPRR574 pKa = 11.84LPAAPDD580 pKa = 3.45YY581 pKa = 9.92TAVAARR587 pKa = 11.84LKK589 pKa = 10.85SILRR593 pKa = 3.86

Molecular weight:
62.37 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A455JQ96|A0A455JQ96_9ALPH Envelope glycoprotein G OS=Cervid alphaherpesvirus 2 OX=365327 GN=US4 PE=4 SV=1
MM1 pKa = 7.6ALARR5 pKa = 11.84TQPPRR10 pKa = 11.84AAGGVFGEE18 pKa = 4.22ARR20 pKa = 11.84VATVADD26 pKa = 4.15YY27 pKa = 11.21KK28 pKa = 11.02RR29 pKa = 11.84LLATNRR35 pKa = 11.84AAAAKK40 pKa = 9.78LRR42 pKa = 11.84AAAGGGEE49 pKa = 4.1AAPDD53 pKa = 3.36EE54 pKa = 4.63RR55 pKa = 11.84GRR57 pKa = 11.84AQTGAGRR64 pKa = 11.84PRR66 pKa = 11.84GGARR70 pKa = 11.84GRR72 pKa = 11.84AAEE75 pKa = 4.27DD76 pKa = 3.41APARR80 pKa = 11.84GARR83 pKa = 11.84RR84 pKa = 11.84PSGRR88 pKa = 11.84AARR91 pKa = 11.84RR92 pKa = 11.84PSGRR96 pKa = 11.84ARR98 pKa = 11.84PAPEE102 pKa = 4.85DD103 pKa = 3.85EE104 pKa = 4.76YY105 pKa = 11.49GDD107 pKa = 3.94EE108 pKa = 4.65YY109 pKa = 11.07EE110 pKa = 5.89DD111 pKa = 3.84EE112 pKa = 4.45YY113 pKa = 11.18EE114 pKa = 4.34YY115 pKa = 11.61GDD117 pKa = 4.11AEE119 pKa = 4.42EE120 pKa = 5.56GPTPAEE126 pKa = 3.69VRR128 pKa = 11.84AARR131 pKa = 11.84LYY133 pKa = 10.75AAAGPARR140 pKa = 11.84ARR142 pKa = 11.84RR143 pKa = 11.84PAPAGEE149 pKa = 4.43FEE151 pKa = 4.15RR152 pKa = 11.84QTRR155 pKa = 11.84AGTRR159 pKa = 3.25

Molecular weight:
16.61 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

68

0

68

37385

88

3326

549.8

57.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

21.391 ± 0.791

1.752 ± 0.124

4.836 ± 0.129

6.144 ± 0.193

3.135 ± 0.183

8.527 ± 0.206

1.765 ± 0.101

1.418 ± 0.108

1.062 ± 0.124

10.304 ± 0.206

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.287 ± 0.114

1.412 ± 0.15

8.083 ± 0.449

2.124 ± 0.148

9.739 ± 0.248

4.307 ± 0.164

3.17 ± 0.162

6.275 ± 0.192

0.995 ± 0.063

2.274 ± 0.19

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski