Cervid alphaherpesvirus 2
Average proteome isoelectric point is 6.86
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 68 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A455JQ30|A0A455JQ30_9ALPH DNA primase OS=Cervid alphaherpesvirus 2 OX=365327 GN=UL52 PE=3 SV=1
MM1 pKa = 7.77 CAPPPPPPPPPSRR14 pKa = 11.84 WLPLLLLLWPLLWPPLGAGAEE35 pKa = 4.16 PGPGALARR43 pKa = 11.84 PVGVIVARR51 pKa = 11.84 AGAPVFLPGDD61 pKa = 3.58 AAVPRR66 pKa = 11.84 PGPAPGPDD74 pKa = 2.86 ARR76 pKa = 11.84 AVRR79 pKa = 11.84 GWSRR83 pKa = 11.84 LASACPPPGPATEE96 pKa = 4.1 VCIDD100 pKa = 3.44 GHH102 pKa = 5.29 QCFADD107 pKa = 3.76 VVVDD111 pKa = 4.31 AACLPTVSLEE121 pKa = 4.01 PLAVAEE127 pKa = 4.16 LSGGGGAGRR136 pKa = 11.84 LADD139 pKa = 3.73 LAEE142 pKa = 4.02 RR143 pKa = 11.84 RR144 pKa = 11.84 VFAQLDD150 pKa = 3.85 YY151 pKa = 11.59 NEE153 pKa = 4.26 TGVWIPNATAADD165 pKa = 3.46 AGVYY169 pKa = 9.57 FLYY172 pKa = 10.54 DD173 pKa = 3.22 RR174 pKa = 11.84 RR175 pKa = 11.84 LGEE178 pKa = 4.19 AGGEE182 pKa = 4.17 EE183 pKa = 4.33 THH185 pKa = 6.58 SALALRR191 pKa = 11.84 VEE193 pKa = 4.29 ADD195 pKa = 3.92 EE196 pKa = 5.03 IEE198 pKa = 4.32 GDD200 pKa = 3.76 EE201 pKa = 4.6 DD202 pKa = 3.58 EE203 pKa = 4.38 EE204 pKa = 4.25 AARR207 pKa = 11.84 GRR209 pKa = 11.84 STAAPRR215 pKa = 11.84 TPAPRR220 pKa = 11.84 TPAAPSPAPARR231 pKa = 11.84 HH232 pKa = 5.59 GARR235 pKa = 11.84 FRR237 pKa = 11.84 VLPYY241 pKa = 9.52 HH242 pKa = 5.24 SHH244 pKa = 6.29 VYY246 pKa = 9.57 TPGDD250 pKa = 3.7 SFQLAVRR257 pKa = 11.84 LQSEE261 pKa = 4.88 FFDD264 pKa = 3.73 EE265 pKa = 4.8 TPFAAAIDD273 pKa = 4.11 WYY275 pKa = 9.92 FLRR278 pKa = 11.84 PAADD282 pKa = 3.52 CALVRR287 pKa = 11.84 LYY289 pKa = 7.34 EE290 pKa = 4.0 TCIFHH295 pKa = 7.43 PEE297 pKa = 3.75 APACLHH303 pKa = 6.34 PVDD306 pKa = 4.85 ARR308 pKa = 11.84 CSFASPYY315 pKa = 10.14 RR316 pKa = 11.84 SEE318 pKa = 4.15 TVYY321 pKa = 10.85 GRR323 pKa = 11.84 LYY325 pKa = 9.87 PRR327 pKa = 11.84 CDD329 pKa = 3.19 PAAGARR335 pKa = 11.84 WPRR338 pKa = 11.84 EE339 pKa = 4.11 CEE341 pKa = 3.97 GASYY345 pKa = 7.92 TAPGAHH351 pKa = 6.72 ARR353 pKa = 11.84 PAEE356 pKa = 4.24 NGADD360 pKa = 3.84 LVFADD365 pKa = 5.18 APAAAAGLYY374 pKa = 10.31 VFVLQYY380 pKa = 10.55 NGHH383 pKa = 5.37 VEE385 pKa = 3.7 AWDD388 pKa = 3.77 YY389 pKa = 11.88 SLVATSDD396 pKa = 3.38 RR397 pKa = 11.84 LVRR400 pKa = 11.84 AVVDD404 pKa = 3.49 HH405 pKa = 6.23 TRR407 pKa = 11.84 PEE409 pKa = 4.04 AAEE412 pKa = 4.55 GPGPSAAPGGGEE424 pKa = 4.17 PASAPAPARR433 pKa = 11.84 RR434 pKa = 11.84 FAPWLVALGGVLALVALGGLAALGVWGCAQRR465 pKa = 11.84 AARR468 pKa = 11.84 RR469 pKa = 11.84 RR470 pKa = 11.84 TYY472 pKa = 10.78 DD473 pKa = 2.73 ILNPFGSAYY482 pKa = 9.76 TSLPTNDD489 pKa = 3.89 PFEE492 pKa = 5.04 SSGEE496 pKa = 4.11 EE497 pKa = 3.74 EE498 pKa = 4.51 GGEE501 pKa = 4.28 DD502 pKa = 4.65 FPPPPGDD509 pKa = 3.43 EE510 pKa = 3.88 TDD512 pKa = 4.65 SFDD515 pKa = 3.89 EE516 pKa = 5.38 DD517 pKa = 3.92 SDD519 pKa = 4.2 EE520 pKa = 4.58 EE521 pKa = 4.11 PSAPYY526 pKa = 10.18 EE527 pKa = 4.12 LADD530 pKa = 4.63 APYY533 pKa = 9.81 EE534 pKa = 4.05 LADD537 pKa = 4.23 AEE539 pKa = 4.43 PAGLARR545 pKa = 11.84 TPAGGPRR552 pKa = 11.84 ASRR555 pKa = 11.84 SGFKK559 pKa = 9.97 VWFRR563 pKa = 11.84 DD564 pKa = 3.65 PVEE567 pKa = 4.55 EE568 pKa = 4.29 EE569 pKa = 4.07 PGSPRR574 pKa = 11.84 LPAAPDD580 pKa = 3.45 YY581 pKa = 9.92 TAVAARR587 pKa = 11.84 LKK589 pKa = 10.85 SILRR593 pKa = 3.86
Molecular weight: 62.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.664
IPC2_protein 4.711
IPC_protein 4.66
Toseland 4.52
ProMoST 4.774
Dawson 4.609
Bjellqvist 4.762
Wikipedia 4.482
Rodwell 4.52
Grimsley 4.431
Solomon 4.609
Lehninger 4.571
Nozaki 4.724
DTASelect 4.876
Thurlkill 4.52
EMBOSS 4.495
Sillero 4.787
Patrickios 3.681
IPC_peptide 4.622
IPC2_peptide 4.787
IPC2.peptide.svr19 4.725
Protein with the highest isoelectric point:
>tr|A0A455JQ96|A0A455JQ96_9ALPH Envelope glycoprotein G OS=Cervid alphaherpesvirus 2 OX=365327 GN=US4 PE=4 SV=1
MM1 pKa = 7.6 ALARR5 pKa = 11.84 TQPPRR10 pKa = 11.84 AAGGVFGEE18 pKa = 4.22 ARR20 pKa = 11.84 VATVADD26 pKa = 4.15 YY27 pKa = 11.21 KK28 pKa = 11.02 RR29 pKa = 11.84 LLATNRR35 pKa = 11.84 AAAAKK40 pKa = 9.78 LRR42 pKa = 11.84 AAAGGGEE49 pKa = 4.1 AAPDD53 pKa = 3.36 EE54 pKa = 4.63 RR55 pKa = 11.84 GRR57 pKa = 11.84 AQTGAGRR64 pKa = 11.84 PRR66 pKa = 11.84 GGARR70 pKa = 11.84 GRR72 pKa = 11.84 AAEE75 pKa = 4.27 DD76 pKa = 3.41 APARR80 pKa = 11.84 GARR83 pKa = 11.84 RR84 pKa = 11.84 PSGRR88 pKa = 11.84 AARR91 pKa = 11.84 RR92 pKa = 11.84 PSGRR96 pKa = 11.84 ARR98 pKa = 11.84 PAPEE102 pKa = 4.85 DD103 pKa = 3.85 EE104 pKa = 4.76 YY105 pKa = 11.49 GDD107 pKa = 3.94 EE108 pKa = 4.65 YY109 pKa = 11.07 EE110 pKa = 5.89 DD111 pKa = 3.84 EE112 pKa = 4.45 YY113 pKa = 11.18 EE114 pKa = 4.34 YY115 pKa = 11.61 GDD117 pKa = 4.11 AEE119 pKa = 4.42 EE120 pKa = 5.56 GPTPAEE126 pKa = 3.69 VRR128 pKa = 11.84 AARR131 pKa = 11.84 LYY133 pKa = 10.75 AAAGPARR140 pKa = 11.84 ARR142 pKa = 11.84 RR143 pKa = 11.84 PAPAGEE149 pKa = 4.43 FEE151 pKa = 4.15 RR152 pKa = 11.84 QTRR155 pKa = 11.84 AGTRR159 pKa = 3.25
Molecular weight: 16.61 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.307
IPC2_protein 9.619
IPC_protein 10.789
Toseland 10.452
ProMoST 10.818
Dawson 10.643
Bjellqvist 10.511
Wikipedia 10.994
Rodwell 10.482
Grimsley 10.745
Solomon 10.847
Lehninger 10.774
Nozaki 10.438
DTASelect 10.511
Thurlkill 10.526
EMBOSS 10.906
Sillero 10.584
Patrickios 10.043
IPC_peptide 10.833
IPC2_peptide 9.692
IPC2.peptide.svr19 8.708
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
68
0
68
37385
88
3326
549.8
57.67
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
21.391 ± 0.791
1.752 ± 0.124
4.836 ± 0.129
6.144 ± 0.193
3.135 ± 0.183
8.527 ± 0.206
1.765 ± 0.101
1.418 ± 0.108
1.062 ± 0.124
10.304 ± 0.206
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.287 ± 0.114
1.412 ± 0.15
8.083 ± 0.449
2.124 ± 0.148
9.739 ± 0.248
4.307 ± 0.164
3.17 ± 0.162
6.275 ± 0.192
0.995 ± 0.063
2.274 ± 0.19
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here