Ralstonia phage phiRSL1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Mieseafarmvirus; Ralstonia virus RSL1

Average proteome isoelectric point is 6.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 343 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B2ZYH7|B2ZYH7_9CAUD DNA-directed DNA polymerase OS=Ralstonia phage phiRSL1 OX=1980924 PE=3 SV=1
MM1 pKa = 7.5TMTDD5 pKa = 2.87TDD7 pKa = 3.99AVSEE11 pKa = 4.5RR12 pKa = 11.84YY13 pKa = 9.06AVNRR17 pKa = 11.84VGEE20 pKa = 4.2LEE22 pKa = 4.1YY23 pKa = 11.06VISDD27 pKa = 4.42LEE29 pKa = 3.85QHH31 pKa = 6.91PYY33 pKa = 8.3ATYY36 pKa = 10.92AVMARR41 pKa = 11.84QVDD44 pKa = 4.21TGWANYY50 pKa = 9.63QILIVEE56 pKa = 4.65LSPASSYY63 pKa = 10.92YY64 pKa = 9.54YY65 pKa = 10.2SIFVRR70 pKa = 11.84EE71 pKa = 4.06VWSNLLFTRR80 pKa = 11.84DD81 pKa = 3.08IEE83 pKa = 4.71RR84 pKa = 11.84ATNWIVSYY92 pKa = 9.41ITTLDD97 pKa = 3.18IADD100 pKa = 4.25PEE102 pKa = 4.07EE103 pKa = 4.31LALQFGGLINNDD115 pKa = 4.37PLPDD119 pKa = 3.82FQVRR123 pKa = 11.84ATTHH127 pKa = 5.29

Molecular weight: 14.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B2ZXM0|B2ZXM0_9CAUD Uncharacterized protein OS=Ralstonia phage phiRSL1 OX=1980924 PE=4 SV=1
MM1 pKa = 7.08PTAPPRR7 pKa = 11.84PLRR10 pKa = 11.84PRR12 pKa = 11.84RR13 pKa = 11.84ARR15 pKa = 11.84RR16 pKa = 11.84SRR18 pKa = 11.84LRR20 pKa = 11.84RR21 pKa = 11.84LLRR24 pKa = 11.84LPRR27 pKa = 11.84RR28 pKa = 11.84LRR30 pKa = 11.84RR31 pKa = 11.84ARR33 pKa = 11.84RR34 pKa = 11.84PLRR37 pKa = 11.84RR38 pKa = 11.84ARR40 pKa = 11.84VRR42 pKa = 11.84RR43 pKa = 11.84PSRR46 pKa = 11.84RR47 pKa = 11.84RR48 pKa = 11.84LVRR51 pKa = 11.84WLPLPRR57 pKa = 11.84RR58 pKa = 11.84APRR61 pKa = 11.84PRR63 pKa = 11.84LPSRR67 pKa = 11.84RR68 pKa = 11.84RR69 pKa = 11.84LRR71 pKa = 11.84RR72 pKa = 11.84AATTSS77 pKa = 2.99

Molecular weight: 9.61 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

343

0

343

74095

40

1586

216.0

23.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.3 ± 0.192

1.055 ± 0.057

5.674 ± 0.163

5.068 ± 0.215

3.626 ± 0.078

6.752 ± 0.21

2.309 ± 0.108

4.284 ± 0.095

5.049 ± 0.219

8.804 ± 0.141

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.499 ± 0.076

4.092 ± 0.119

5.183 ± 0.112

4.685 ± 0.106

5.911 ± 0.21

6.54 ± 0.19

6.89 ± 0.309

7.582 ± 0.148

1.517 ± 0.062

3.18 ± 0.085
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski