Hubei partiti-like virus 11
Average proteome isoelectric point is 6.61
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KLP6|A0A1L3KLP6_9VIRU RdRp OS=Hubei partiti-like virus 11 OX=1923017 PE=4 SV=1
MM1 pKa = 7.86 EE2 pKa = 4.69 YY3 pKa = 10.33 LGKK6 pKa = 10.51 AEE8 pKa = 4.47 GFNMDD13 pKa = 4.36 LRR15 pKa = 11.84 PPRR18 pKa = 11.84 MDD20 pKa = 5.92 GITCGYY26 pKa = 8.48 MNDD29 pKa = 3.84 WFGDD33 pKa = 3.61 AYY35 pKa = 11.26 VDD37 pKa = 3.94 VVMQTYY43 pKa = 9.99 HH44 pKa = 7.24 RR45 pKa = 11.84 SQPSLEE51 pKa = 4.01 LLKK54 pKa = 10.65 EE55 pKa = 3.72 DD56 pKa = 4.28 FMRR59 pKa = 11.84 YY60 pKa = 8.31 DD61 pKa = 3.34 RR62 pKa = 11.84 KK63 pKa = 10.51 YY64 pKa = 8.62 VAGTVRR70 pKa = 11.84 EE71 pKa = 4.08 EE72 pKa = 3.48 QSYY75 pKa = 11.03 RR76 pKa = 11.84 MVLQSVRR83 pKa = 11.84 DD84 pKa = 3.67 QFVLGEE90 pKa = 4.04 KK91 pKa = 10.38 LIPLTLGAVFEE102 pKa = 4.92 SNSMTTDD109 pKa = 3.46 KK110 pKa = 11.42 SPGLPWIQRR119 pKa = 11.84 GYY121 pKa = 8.11 KK122 pKa = 9.04 TKK124 pKa = 10.95 GDD126 pKa = 3.35 VFACHH131 pKa = 6.0 EE132 pKa = 4.18 ARR134 pKa = 11.84 SEE136 pKa = 4.74 IFRR139 pKa = 11.84 TWDD142 pKa = 3.55 RR143 pKa = 11.84 IGNGRR148 pKa = 11.84 GATLPDD154 pKa = 3.02 TCAFMRR160 pKa = 11.84 VQLAKK165 pKa = 10.3 EE166 pKa = 4.22 DD167 pKa = 3.75 KK168 pKa = 10.22 QKK170 pKa = 10.44 IRR172 pKa = 11.84 AVWGTPTDD180 pKa = 4.05 VIAEE184 pKa = 4.22 EE185 pKa = 4.29 ARR187 pKa = 11.84 FFLPYY192 pKa = 8.82 MAKK195 pKa = 10.4 LKK197 pKa = 11.05 LSDD200 pKa = 3.67 APIAYY205 pKa = 9.0 RR206 pKa = 11.84 AEE208 pKa = 4.08 MATGGMSLINDD219 pKa = 4.21 MCQSHH224 pKa = 7.36 PGAKK228 pKa = 10.12 YY229 pKa = 10.97 LMTDD233 pKa = 3.74 LSQFDD238 pKa = 3.79 KK239 pKa = 11.16 SVPPWLIRR247 pKa = 11.84 DD248 pKa = 3.46 AFGIVMEE255 pKa = 4.8 NFDD258 pKa = 3.75 FTRR261 pKa = 11.84 VVGSDD266 pKa = 3.21 GKK268 pKa = 10.97 VWDD271 pKa = 4.15 VNPDD275 pKa = 2.98 RR276 pKa = 11.84 TKK278 pKa = 10.91 RR279 pKa = 11.84 RR280 pKa = 11.84 ISRR283 pKa = 11.84 MIDD286 pKa = 3.12 YY287 pKa = 9.08 FINTPVRR294 pKa = 11.84 LCNGEE299 pKa = 4.1 RR300 pKa = 11.84 YY301 pKa = 9.26 RR302 pKa = 11.84 KK303 pKa = 9.9 RR304 pKa = 11.84 GGVPSGSMWTNIVDD318 pKa = 4.49 TIVNAIISRR327 pKa = 11.84 YY328 pKa = 10.13 CIFNTSGRR336 pKa = 11.84 LPLADD341 pKa = 4.46 MYY343 pKa = 11.55 LGDD346 pKa = 4.22 DD347 pKa = 3.36 QFAVVDD353 pKa = 5.19 GIINLNDD360 pKa = 3.02 IAKK363 pKa = 10.4 LMNEE367 pKa = 4.05 SFGMVLHH374 pKa = 7.17 PDD376 pKa = 2.87 KK377 pKa = 11.07 CVYY380 pKa = 9.44 TEE382 pKa = 3.91 NASNVQFLGYY392 pKa = 9.69 FNRR395 pKa = 11.84 NGLPWKK401 pKa = 10.88 GNMFLVASMIFPEE414 pKa = 5.04 RR415 pKa = 11.84 PVNDD419 pKa = 2.61 NMTRR423 pKa = 11.84 VSRR426 pKa = 11.84 AIGQMWSTLHH436 pKa = 6.64 GGQAVRR442 pKa = 11.84 WWNIVQAMLRR452 pKa = 11.84 DD453 pKa = 4.0 FGFTSKK459 pKa = 10.64 EE460 pKa = 3.94 VIEE463 pKa = 5.1 NIQSRR468 pKa = 11.84 PGSFRR473 pKa = 11.84 YY474 pKa = 10.22 LRR476 pKa = 11.84 MLGIDD481 pKa = 3.18 ISKK484 pKa = 10.22 IGIPTRR490 pKa = 11.84 YY491 pKa = 9.75 GEE493 pKa = 4.63 YY494 pKa = 10.0 ILGIDD499 pKa = 4.66 PPWKK503 pKa = 9.62 SLKK506 pKa = 10.27 SYY508 pKa = 10.95 RR509 pKa = 11.84 PVKK512 pKa = 9.97 WDD514 pKa = 3.18 IDD516 pKa = 3.8 DD517 pKa = 4.28 LDD519 pKa = 4.45 RR520 pKa = 11.84 RR521 pKa = 11.84 SQSIDD526 pKa = 2.99 MEE528 pKa = 4.13 EE529 pKa = 3.46 WHH531 pKa = 6.98 NFIQCMDD538 pKa = 4.49 DD539 pKa = 4.37 SDD541 pKa = 5.99 DD542 pKa = 3.73 EE543 pKa = 4.4
Molecular weight: 62.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.963
IPC2_protein 6.033
IPC_protein 6.097
Toseland 6.262
ProMoST 6.249
Dawson 6.376
Bjellqvist 6.376
Wikipedia 6.376
Rodwell 6.364
Grimsley 6.338
Solomon 6.389
Lehninger 6.376
Nozaki 6.634
DTASelect 6.81
Thurlkill 6.795
EMBOSS 6.781
Sillero 6.737
Patrickios 4.304
IPC_peptide 6.402
IPC2_peptide 6.59
IPC2.peptide.svr19 6.667
Protein with the highest isoelectric point:
>tr|A0A1L3KLP6|A0A1L3KLP6_9VIRU RdRp OS=Hubei partiti-like virus 11 OX=1923017 PE=4 SV=1
MM1 pKa = 6.84 MSEE4 pKa = 4.29 RR5 pKa = 11.84 SHH7 pKa = 7.23 LNLKK11 pKa = 9.64 MNTDD15 pKa = 3.75 KK16 pKa = 10.4 KK17 pKa = 9.18 TKK19 pKa = 10.41 SKK21 pKa = 10.91 GPKK24 pKa = 8.39 KK25 pKa = 8.56 TSKK28 pKa = 10.33 PKK30 pKa = 9.97 EE31 pKa = 4.15 EE32 pKa = 4.15 KK33 pKa = 10.27 KK34 pKa = 10.83 EE35 pKa = 3.97 PVDD38 pKa = 3.73 APSVKK43 pKa = 10.41 VEE45 pKa = 3.95 VEE47 pKa = 3.95 KK48 pKa = 10.81 PKK50 pKa = 10.05 PAEE53 pKa = 3.87 VTKK56 pKa = 9.92 PAEE59 pKa = 4.18 PVKK62 pKa = 9.37 PTPAPQKK69 pKa = 10.21 SKK71 pKa = 10.88 PKK73 pKa = 9.97 AQSQVASKK81 pKa = 10.27 LQNDD85 pKa = 4.58 PLWEE89 pKa = 4.57 DD90 pKa = 3.89 FGSDD94 pKa = 3.91 DD95 pKa = 5.06 FDD97 pKa = 3.93 VLMPEE102 pKa = 4.19 RR103 pKa = 11.84 GDD105 pKa = 3.2 NRR107 pKa = 11.84 FAVTLEE113 pKa = 4.15 GYY115 pKa = 9.98 IPLIEE120 pKa = 4.1 QTYY123 pKa = 7.64 EE124 pKa = 4.1 TLSHH128 pKa = 6.56 EE129 pKa = 4.86 DD130 pKa = 3.16 KK131 pKa = 11.07 TFGKK135 pKa = 10.58 SMSLSMFSWYY145 pKa = 10.02 CCQHH149 pKa = 7.34 LYY151 pKa = 9.81 MRR153 pKa = 11.84 IITIRR158 pKa = 11.84 MSEE161 pKa = 4.09 GLATSDD167 pKa = 3.16 EE168 pKa = 4.07 KK169 pKa = 11.21 RR170 pKa = 11.84 YY171 pKa = 9.14 VAEE174 pKa = 3.98 VSSIPFVIPLTIEE187 pKa = 4.07 IYY189 pKa = 10.57 LKK191 pKa = 10.9 SIGNVMDD198 pKa = 4.91 AEE200 pKa = 4.37 LNKK203 pKa = 10.78 LKK205 pKa = 10.44 ISFPAFPNAEE215 pKa = 3.84 GHH217 pKa = 6.62 FGVVNNNSHH226 pKa = 5.89 WKK228 pKa = 9.17 YY229 pKa = 10.08 EE230 pKa = 4.44 SVPAPYY236 pKa = 8.71 VTSRR240 pKa = 11.84 RR241 pKa = 11.84 MIHH244 pKa = 7.12 DD245 pKa = 3.58 YY246 pKa = 10.97 AYY248 pKa = 7.37 TTGLEE253 pKa = 4.0 NDD255 pKa = 5.04 RR256 pKa = 11.84 IWTLPLNLLPPNRR269 pKa = 11.84 DD270 pKa = 3.0 ATRR273 pKa = 11.84 HH274 pKa = 6.06 RR275 pKa = 11.84 ICGNPTKK282 pKa = 10.81 NLLGWARR289 pKa = 11.84 SSMLTSEE296 pKa = 3.87 QTLILEE302 pKa = 4.39 SCGIDD307 pKa = 3.3 GANFPTEE314 pKa = 3.46 RR315 pKa = 11.84 PMFQFNRR322 pKa = 11.84 GLFVLLSSHH331 pKa = 6.87 LSAASRR337 pKa = 11.84 SMKK340 pKa = 9.88 MSGSAITSIEE350 pKa = 3.92 GSFGQTLFVEE360 pKa = 4.67 RR361 pKa = 11.84 DD362 pKa = 3.28 DD363 pKa = 3.94 RR364 pKa = 11.84 HH365 pKa = 6.58 PEE367 pKa = 3.7 EE368 pKa = 4.46 FDD370 pKa = 4.87 RR371 pKa = 11.84 NVQYY375 pKa = 11.36 CEE377 pKa = 3.88 MGNLCTVSAFQVDD390 pKa = 3.36 KK391 pKa = 11.06 RR392 pKa = 11.84 FSQGAAVCNFRR403 pKa = 11.84 SRR405 pKa = 11.84 KK406 pKa = 9.6 FEE408 pKa = 4.22 INNLRR413 pKa = 11.84 SYY415 pKa = 11.6 ACFDD419 pKa = 3.71 FGDD422 pKa = 4.3 YY423 pKa = 10.96 EE424 pKa = 4.28 HH425 pKa = 7.73 VPDD428 pKa = 4.06 GWNNTVNTIFDD439 pKa = 4.36 FGAQADD445 pKa = 3.77 WNRR448 pKa = 11.84 PKK450 pKa = 9.86 FTSAYY455 pKa = 7.93 GSKK458 pKa = 10.31 VALRR462 pKa = 11.84 QSWVKK467 pKa = 10.56 KK468 pKa = 10.24 SLVRR472 pKa = 11.84 DD473 pKa = 3.56 KK474 pKa = 10.99 TT475 pKa = 3.62
Molecular weight: 53.9 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.257
IPC2_protein 7.688
IPC_protein 7.556
Toseland 7.307
ProMoST 8.112
Dawson 8.302
Bjellqvist 8.58
Wikipedia 8.244
Rodwell 8.346
Grimsley 7.337
Solomon 8.39
Lehninger 8.404
Nozaki 8.799
DTASelect 8.361
Thurlkill 8.434
EMBOSS 8.521
Sillero 8.726
Patrickios 4.584
IPC_peptide 8.39
IPC2_peptide 7.41
IPC2.peptide.svr19 7.682
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1018
475
543
509.0
58.15
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.501 ± 0.256
1.572 ± 0.073
6.778 ± 0.986
5.599 ± 0.74
5.01 ± 0.165
6.385 ± 0.867
1.572 ± 0.347
5.501 ± 0.84
5.992 ± 1.032
6.974 ± 0.119
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
4.224 ± 0.694
5.01 ± 0.302
5.501 ± 0.53
3.143 ± 0.128
6.68 ± 0.785
7.564 ± 1.106
5.206 ± 0.448
6.189 ± 0.191
2.161 ± 0.31
3.438 ± 0.319
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here