Lactobacillus phage PM411

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 91 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2P0ZLF3|A0A2P0ZLF3_9CAUD ATP-dependent DNA helicase OS=Lactobacillus phage PM411 OX=2079298 PE=4 SV=1
MM1 pKa = 7.78LIKK4 pKa = 9.62IDD6 pKa = 3.63EE7 pKa = 4.38TLAKK11 pKa = 9.64QVCDD15 pKa = 3.67EE16 pKa = 4.16LHH18 pKa = 6.68IDD20 pKa = 3.65EE21 pKa = 5.05TDD23 pKa = 3.3EE24 pKa = 3.98EE25 pKa = 5.0LATVTALLNGSQAIIDD41 pKa = 4.22DD42 pKa = 4.55SIEE45 pKa = 3.78LAIYY49 pKa = 9.63PDD51 pKa = 3.61IVDD54 pKa = 3.49NPLYY58 pKa = 9.85TRR60 pKa = 11.84AIITLCQSLYY70 pKa = 10.35YY71 pKa = 10.37DD72 pKa = 3.9RR73 pKa = 11.84NLATGQPKK81 pKa = 10.2AVILMVDD88 pKa = 4.16HH89 pKa = 7.0LNSICLAKK97 pKa = 10.49EE98 pKa = 3.45ADD100 pKa = 3.72TDD102 pKa = 3.95GSKK105 pKa = 10.9

Molecular weight: 11.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2P0ZL72|A0A2P0ZL72_9CAUD Uncharacterized protein OS=Lactobacillus phage PM411 OX=2079298 PE=4 SV=1
MM1 pKa = 7.28ITNKK5 pKa = 9.5CRR7 pKa = 11.84YY8 pKa = 9.01GLVGLISIVLTFGLSASNRR27 pKa = 11.84LRR29 pKa = 11.84ASFDD33 pKa = 3.13AMPAITYY40 pKa = 6.7MSKK43 pKa = 10.86YY44 pKa = 10.43GIINHH49 pKa = 5.41RR50 pKa = 11.84TQWQAMLHH58 pKa = 5.38ATDD61 pKa = 4.16HH62 pKa = 5.78GRR64 pKa = 11.84KK65 pKa = 8.03ITSLTQPNQILRR77 pKa = 11.84AANLHH82 pKa = 5.85SFAVANLRR90 pKa = 11.84QVEE93 pKa = 4.43HH94 pKa = 6.4ATRR97 pKa = 11.84PTKK100 pKa = 10.38TMSAGLTIINVPAFYY115 pKa = 10.57SSQHH119 pKa = 5.13QLQKK123 pKa = 10.91QYY125 pKa = 7.86WTQLQHH131 pKa = 7.42LIKK134 pKa = 9.2QTKK137 pKa = 7.09PTTDD141 pKa = 4.59LILNFAANTGGSSAPMIAGLAAIIPDD167 pKa = 3.39GTLYY171 pKa = 10.58TGITNHH177 pKa = 5.75HH178 pKa = 6.56RR179 pKa = 11.84NRR181 pKa = 11.84PRR183 pKa = 11.84MMYY186 pKa = 9.89HH187 pKa = 5.69GRR189 pKa = 11.84ITGGLGQDD197 pKa = 4.5DD198 pKa = 5.28FNFSQPTAAHH208 pKa = 6.17FRR210 pKa = 11.84HH211 pKa = 6.73IYY213 pKa = 10.41AIIGNQTASAAEE225 pKa = 4.06VTIMALKK232 pKa = 10.36RR233 pKa = 11.84NPHH236 pKa = 4.61VTLVGRR242 pKa = 11.84SSAGYY247 pKa = 7.67TSLNGGVFLNTAVAVMTIGTLKK269 pKa = 10.11ATVKK273 pKa = 10.21IHH275 pKa = 5.12GQQYY279 pKa = 10.51FNNQPLKK286 pKa = 10.47PDD288 pKa = 3.5IPTLYY293 pKa = 10.41QPLAPLQLGQSPHH306 pKa = 7.24LDD308 pKa = 3.14ADD310 pKa = 3.98FLSEE314 pKa = 4.2LRR316 pKa = 11.84TIMKK320 pKa = 9.86HH321 pKa = 3.86YY322 pKa = 10.27HH323 pKa = 5.11

Molecular weight: 35.62 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

91

0

91

21326

39

1583

234.4

25.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.492 ± 0.427

0.441 ± 0.07

6.771 ± 0.31

5.022 ± 0.315

3.662 ± 0.198

7.034 ± 0.433

1.749 ± 0.126

6.176 ± 0.238

7.428 ± 0.445

7.85 ± 0.215

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.466 ± 0.159

5.688 ± 0.216

3.006 ± 0.218

4.628 ± 0.174

3.475 ± 0.242

7.066 ± 0.389

7.568 ± 0.346

6.476 ± 0.162

1.224 ± 0.144

3.779 ± 0.242
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski