Gordonia phage Kenna
Average proteome isoelectric point is 6.37
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 98 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3S9UPU1|A0A3S9UPU1_9CAUD Minor tail protein OS=Gordonia phage Kenna OX=2499025 GN=34 PE=4 SV=1
MM1 pKa = 7.77 SEE3 pKa = 3.82 WLDD6 pKa = 4.28 DD7 pKa = 3.44 NGTRR11 pKa = 11.84 HH12 pKa = 5.16 WTDD15 pKa = 2.95 DD16 pKa = 3.7 DD17 pKa = 3.83 GRR19 pKa = 11.84 EE20 pKa = 4.18 HH21 pKa = 7.18 VDD23 pKa = 3.31 LDD25 pKa = 3.51 ASQEE29 pKa = 3.89 LRR31 pKa = 11.84 IDD33 pKa = 3.65 FQMSQTRR40 pKa = 11.84 STKK43 pKa = 9.31 EE44 pKa = 3.43 AAPEE48 pKa = 3.95 FGDD51 pKa = 3.76 TNNLDD56 pKa = 4.02 EE57 pKa = 4.7 EE58 pKa = 4.8 WVEE61 pKa = 3.79 EE62 pKa = 4.97 PYY64 pKa = 10.95 EE65 pKa = 4.22 HH66 pKa = 7.29 WCPGLPYY73 pKa = 10.27 CDD75 pKa = 3.08 TCGRR79 pKa = 11.84 HH80 pKa = 5.77 YY81 pKa = 10.81 MGDD84 pKa = 3.43 DD85 pKa = 3.0 WW86 pKa = 6.69
Molecular weight: 10.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.982
IPC2_protein 4.024
IPC_protein 3.973
Toseland 3.783
ProMoST 4.139
Dawson 3.961
Bjellqvist 4.113
Wikipedia 3.884
Rodwell 3.808
Grimsley 3.694
Solomon 3.948
Lehninger 3.91
Nozaki 4.075
DTASelect 4.291
Thurlkill 3.821
EMBOSS 3.897
Sillero 4.101
Patrickios 2.842
IPC_peptide 3.948
IPC2_peptide 4.075
IPC2.peptide.svr19 3.994
Protein with the highest isoelectric point:
>tr|A0A3S9UPU9|A0A3S9UPU9_9CAUD Helix-turn-helix DNA binding protein OS=Gordonia phage Kenna OX=2499025 GN=43 PE=4 SV=1
MM1 pKa = 7.0 NTIKK5 pKa = 10.78 DD6 pKa = 3.5 RR7 pKa = 11.84 GIQASVVTAGILAVVTAVAYY27 pKa = 9.69 SPPLLAVWAVWAGVTVWAIYY47 pKa = 7.23 EE48 pKa = 4.03 ARR50 pKa = 11.84 RR51 pKa = 11.84 KK52 pKa = 9.0 HH53 pKa = 6.15 ARR55 pKa = 11.84 AA56 pKa = 3.49
Molecular weight: 6.02 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.302
IPC2_protein 9.911
IPC_protein 10.687
Toseland 10.54
ProMoST 10.292
Dawson 10.701
Bjellqvist 10.423
Wikipedia 10.921
Rodwell 10.921
Grimsley 10.774
Solomon 10.789
Lehninger 10.76
Nozaki 10.496
DTASelect 10.423
Thurlkill 10.555
EMBOSS 10.935
Sillero 10.613
Patrickios 10.804
IPC_peptide 10.789
IPC2_peptide 9.297
IPC2.peptide.svr19 8.37
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
98
0
98
16776
41
1875
171.2
18.8
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.998 ± 0.484
1.085 ± 0.182
6.575 ± 0.261
6.003 ± 0.292
2.724 ± 0.199
8.178 ± 0.428
2.253 ± 0.18
4.524 ± 0.181
3.588 ± 0.312
7.57 ± 0.248
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.337 ± 0.189
2.867 ± 0.166
5.526 ± 0.231
3.594 ± 0.166
7.409 ± 0.411
5.77 ± 0.279
6.605 ± 0.392
7.648 ± 0.324
2.289 ± 0.127
2.456 ± 0.197
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here